PHYTO-PAM-IIMultiple Excitation Wavelength Phytoplankton & Photosynthesis Analyzer

Five measuring light wave­lengths, six actinic light colors

Accurate analysis of individualalgal groups occurring in amixed algal sample of e.g.green algae, cyanobacteria,diatoms/dinoflagellates andphycoerythrin­containing algae

Highly sensitive fluorescencedetection by photomultiplier

Full range of saturation pulseanalysis

Fast Kinetic recordings anddetermination of the functionalantenna size of PS II

Automated recordings and script file operation

Available as COMPACT or MODULAR version

MONITORING-PAMMulti-Channel Chlorophyll Fluorometer

Specially developed for long­term monitoring of chlorophyllfluorescence

Connections for 7 measuring heads provided by the stand­ alone Data Acquisition System MONI­DA. Upgradable to 16 measuring heads using special multichannel interfaces

Powered by battery and solar panels

Robust and waterproof components for unattended operation in the field. Underwater version for aquatic plants available

Optional data transfer via tele­ phone modem or satellite phone

DIVING-PAM-IIUnderwater Fluorometer with Miniature Spectrometer

Miniature spectrometer included to record spectra of PAR, reflectance and fluorescence

High power LED for actinic light and saturation pulses. Far red LED for PS I excitation

Wireless LAN for data download. Built­in PAR sensor. Low­power consuming transflective B/W screen

High capacity battery for more than 2,000 PS II yield measure­ments. Flash memory for more than 27,000 saturation pulse analyses

Automatic calculation of all relevant parameters of saturation pulse analysis

Software and many accessories included

The COMPACT version of the PHYTO­PAM­II is easily portable due to integration of light sources, fluorescence detection and signal processing in the same housing. The device offers variability in actinic light colors and extended analysis options for detailed assessment of photosynthetic performance – even for the very dilute algal concentrations in natural water samples.

The MODULAR version provides PHYTO­PAM­II features known from the COMPACT version in a more flexible and extendable system.

Next to exchangeable detector filters and two additional optical ports this version provides options for software triggered stirring and temperature control.

The ONLINE configuration ofthe MONITORING­PAM simplyrequires an interface box fordata acquisition by a WindowsPC running the WinControl­3software. In the absence ofline current, a dedicated DataAcquisition System allowsbattery-operated fluorescencemonitoring. Solar panels areprovided to recharge batteries.

Standard measuring headsmeasure photosyntheticallyactive radiation (PAR) by aninternal sensor. The newly de­veloped ED measuring headsrecord PAR by an external cosine response sensor.

Elec tron transport rates are derived from PAM fluorescence and PAR to assess long­term performance of plants.

The predecessor of the DIVING­PAM­II (the DIVING­PAM) has been proven as reliable and robust chlorophyll fluorometer for studying photosynthesis of water plants.

The DIVING­PAM­II continues the proven and trusted design of its predecessor but advances data acquisition and instrument control by employing the latest progress in optics and electronics.

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Heinz Walz GmbHEichenring 6 · 91090 Effeltrich · GermanyTel. +49(0)9133/7765­0 · Fax +49(0)9133/5395info@walz.com · www.walz.com

Data Acquisition System

Measuring HeadTemperature Control Unit

MINI-PAM-IIPhotosynthesis Yield Analyzer

Ultra­mobile, compact and reliable PAM fluorometer for field use

Easy operation via touch­screen interface

Versions with blue or red light available

Strong far­red light source for photosystem I excitation

Leaf clip with improved sensor of photosynthetically active radiation (PAR)

Unique expandability of experimental options by add­ons like high­power RGBW lamp, compact spectrometer (for light, fluorescence and reflectance spectra), or oxygen package for suspensions

The MINI-PAM-II fluorometer combines the merits of its predecessor “MINI­PAM” with most recent LED and computer technology. A trans flective touchscreen, energy efficient LED sources and easy replace ­ able off-the-shelf batteries make the MINI­PAM­II the ideal PAM fluorometer for field research. All common fluor -es cence parameters are auto­ma tically calculated.

Photosynthetic electron trans­port (ETR) under field conditionscan be derived from PAR measurements by the leaf clip and PAM fluorescence. When photosynthesis is driven bythe MINI­PAM­II internal lightsource, PAR data for ETR calculations are measured by an internal light sensor.The MINI­PAM­II can be oper­ated by Windows computersusing WinControl­3 software.

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DIVING-PAM-IIExternal LED Light Source

Leaf-Clip HolderCOMPACT version

PRODUCTHIGHLIGHTS

NEW:DIVING-PAM-II

Precision Analysis of Photosynthesis

The PAM­2500 Portable Chloro­phyll Fluorometer succeedsthe renowned PAM­2000/2100 instruments which were intro­duced in the 1990s as the first portable PAM fluorometers.In the development of the PAM­2500, particular care was taken to maintain all properties appreciated by PAM­2000/2100 users and, at the same time, to integrate state­of­the­art technology.

Compared to its predecessors,PAM­2500 consistently employs LED technology for saturation pulses, actinic light, single and multiple turnover flashes.

Also, the PAM­2500 exhibits amuch improved time resolutiondown to 10 μs and significantlyhigher sensitivity for high qualitymeasurements even with critical samples (e.g. low Chl content).

IMAG-3DChlorophyll Fluorescence System

Compact 3D system based on MAXI Version of the IMAGING­PAM

3D imaging through the highly innovative Stripe­Pattern­Analysis by the Fraunhofer Institute for Integrated Circuits IIS

Photosynthetic parameters projected on three­dimensional image

Additional morphological para­meters for plant phenotyping

Excellently suited for Arabi­dopsis plants

The IMAG­3D system is based on the successful MAXI IMAGING­PAM to which a 3D scanner is added.

Using algorithms developed by the Fraunhofer Institute for Integrated Circuits IIS, the system creates a 3D mesh from images of structured light casted on the plant by a projector.

For 3D imaging, the camera is used in the high resolution mode (1280 x 960 pixels) to precisely digitize the sample surface.

A dedicated tabletop housing offers ideal darkening for 3D and PSII analysis.

Evaluation of leaf morphology

3D image of qN parameter

PAM-2500Portable Chlorophyll Fluorometer

For basic/applied researchand plant screening

Powerful illumination systemfeaturing red, blue, far­redlight and single/multiple turn­over flashes

Fully computer controlled;optional ultra­mobile PC fortouch screen operation

User­friendly and versatilePamWin­3 software forquenching analysis, slow/fastkinetic recordings, and lightcurves

New 2030­B Leaf­Clip Holderwith a still more accurate PAR sensor

Optional accessories for algae and cyanobacteria

MULTI-COLOR-PAMMultiple Excitation Wavelength Chlorophyll Fluorometer

Unprecedented range ofcolors of measuring andactinic light including whiteand far­red

Highly sensitive analysisof dilute chloroplasts or cellsuspensions

Accessory for leaf studiesincluding evaluation ofepidermal UV­A screening

Saturation Pulse analysis andfast kinetics recordings with10 μs time resolution

Highly accurate determinationof effective PS II quantum yieldeven under severe light­stress

Determination of functionalPS II absorption cross­sectionand PS II turnover rates

Optical unit for leaf measurements

Optical unit for suspensions

The MULTI­COLOR­PAM provides six colors of pulse­modulated measuring light (365 or 400, 440, 480, 540, 590 and625 nm) and five colors of actinic light (440, 480, 540, 590,625) plus white (420­640 nm)and far­red light (730 nm).

The various colors enableadaptation of excitation light tothe spectral properties of the

sample and to study wave­length­dependent aspects of photosynthesis.

Spherical and flat cosine-corrected sensors are availablefor accurate PAR­measurementsin suspension or at leaf surface, respectively. A special routine is provided for measuring PAR­lists of all colors.

The GFS­3000 is a high precision gas exchange and chlorophyll fluorescence measuring system. The instrument fully controls CO2 and H2O concentrations, cuvette or leaf temperature, ventilation and illumination.

The new LED­Array 3041­L provides homogeneous warm white illumination from 1 to ca. 3000 μmol m­2 s­1 PAR on up to 10 cm2 sample area.

Also, the new LED­Array/PAM­Fluorometer 3056­FL, providinglight control and simultaneouschlorophyll fluorescencemeasurements, excels by itshomogeneity of illumination.

The GFS­3000 can be combined with IMAGING­PAM or DUAL­ PAM­100 for simultaneous measurements, analyzing photo­synthesis at an elevated level of information.

GFS-3000Portable Gas Exchange & Fluorescence System

Integrated H2O control fordrying and humidifying (0 tonearly 100% rh)

Integrated CO2 control (0 to2000 ppm)

Powerful temperature control

Simultaneous chlorophyll fluorescence measurements

Flexible applications by various cuvette options

Gas­Exchange Chamber 3010­GWK1 and LED­Panel RGBW­L084 for medium sized samples

Extensive analysis options by combination with other Walzsystems

New: 3041-L and 3056-FL

3010-GWK1 with LED-Panel

DUAL-PAM-100P700 & Chlorophyll Fluorescence System

Simultaneous measurementsof Chl fluorescence and P700 absorbance changes due to innovative dual­channel modulation technique

Fiber version available

Integrated red, blue, andfar­red actinic illumination.Saturating single turnover andmultiple turnover flashes

Dedicated DualPAM softwarefeaturing numerous automatedmeasuring routines

Saturation pulse method forsimultaneous assessment ofPS I and PS II quantum yields

Optional emitter­detectorheads for measuring additionalphotosynthetic parameters(ΔpH, NADPH, P515 etc.)

The DUAL­PAM­100 may serve for advanced analysis of photosynthesis parameters in intact leaves and suspensions of algae/cyanobacteria/iso lated chloroplasts. PS I is assessed via dual­wavelength P700 measurements (830 vs. 870 nm absorbance) using essentially the same saturation pulse method as for assessment of PS II via Chl fluorescence.

All light sources are integratedin an extremely compactoptical system and controlled via the dedicated DualPAM windows software with 2.5 μs resolution.

Preprogrammed measuringroutines allow complex proto cols to be carried out repro ducibly even by non­experts.

Measuring setup for suspensions

Leaf holder Laboratory setup

Leaf Clip