Multiple Excitation Wavelength Phytoplankton Multi-Channel … · The PAM2500 Portable Chloro phyll...
Transcript of Multiple Excitation Wavelength Phytoplankton Multi-Channel … · The PAM2500 Portable Chloro phyll...
PHYTO-PAM-IIMultiple Excitation Wavelength Phytoplankton & Photosynthesis Analyzer
Five measuring light wavelengths, six actinic light colors
Accurate analysis of individualalgal groups occurring in amixed algal sample of e.g.green algae, cyanobacteria,diatoms/dinoflagellates andphycoerythrincontaining algae
Highly sensitive fluorescencedetection by photomultiplier
Full range of saturation pulseanalysis
Fast Kinetic recordings anddetermination of the functionalantenna size of PS II
Automated recordings and script file operation
Available as COMPACT or MODULAR version
MONITORING-PAMMulti-Channel Chlorophyll Fluorometer
Specially developed for longterm monitoring of chlorophyllfluorescence
Connections for 7 measuring heads provided by the stand alone Data Acquisition System MONIDA. Upgradable to 16 measuring heads using special multichannel interfaces
Powered by battery and solar panels
Robust and waterproof components for unattended operation in the field. Underwater version for aquatic plants available
Optional data transfer via tele phone modem or satellite phone
DIVING-PAM-IIUnderwater Fluorometer with Miniature Spectrometer
Miniature spectrometer included to record spectra of PAR, reflectance and fluorescence
High power LED for actinic light and saturation pulses. Far red LED for PS I excitation
Wireless LAN for data download. Builtin PAR sensor. Lowpower consuming transflective B/W screen
High capacity battery for more than 2,000 PS II yield measurements. Flash memory for more than 27,000 saturation pulse analyses
Automatic calculation of all relevant parameters of saturation pulse analysis
Software and many accessories included
The COMPACT version of the PHYTOPAMII is easily portable due to integration of light sources, fluorescence detection and signal processing in the same housing. The device offers variability in actinic light colors and extended analysis options for detailed assessment of photosynthetic performance – even for the very dilute algal concentrations in natural water samples.
The MODULAR version provides PHYTOPAMII features known from the COMPACT version in a more flexible and extendable system.
Next to exchangeable detector filters and two additional optical ports this version provides options for software triggered stirring and temperature control.
The ONLINE configuration ofthe MONITORINGPAM simplyrequires an interface box fordata acquisition by a WindowsPC running the WinControl3software. In the absence ofline current, a dedicated DataAcquisition System allowsbattery-operated fluorescencemonitoring. Solar panels areprovided to recharge batteries.
Standard measuring headsmeasure photosyntheticallyactive radiation (PAR) by aninternal sensor. The newly developed ED measuring headsrecord PAR by an external cosine response sensor.
Elec tron transport rates are derived from PAM fluorescence and PAR to assess longterm performance of plants.
The predecessor of the DIVINGPAMII (the DIVINGPAM) has been proven as reliable and robust chlorophyll fluorometer for studying photosynthesis of water plants.
The DIVINGPAMII continues the proven and trusted design of its predecessor but advances data acquisition and instrument control by employing the latest progress in optics and electronics.
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Heinz Walz GmbHEichenring 6 · 91090 Effeltrich · GermanyTel. +49(0)9133/77650 · Fax +49(0)9133/[email protected] · www.walz.com
Data Acquisition System
Measuring HeadTemperature Control Unit
MINI-PAM-IIPhotosynthesis Yield Analyzer
Ultramobile, compact and reliable PAM fluorometer for field use
Easy operation via touchscreen interface
Versions with blue or red light available
Strong farred light source for photosystem I excitation
Leaf clip with improved sensor of photosynthetically active radiation (PAR)
Unique expandability of experimental options by addons like highpower RGBW lamp, compact spectrometer (for light, fluorescence and reflectance spectra), or oxygen package for suspensions
The MINI-PAM-II fluorometer combines the merits of its predecessor “MINIPAM” with most recent LED and computer technology. A trans flective touchscreen, energy efficient LED sources and easy replace able off-the-shelf batteries make the MINIPAMII the ideal PAM fluorometer for field research. All common fluor -es cence parameters are automa tically calculated.
Photosynthetic electron transport (ETR) under field conditionscan be derived from PAR measurements by the leaf clip and PAM fluorescence. When photosynthesis is driven bythe MINIPAMII internal lightsource, PAR data for ETR calculations are measured by an internal light sensor.The MINIPAMII can be operated by Windows computersusing WinControl3 software.
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DIVING-PAM-IIExternal LED Light Source
Leaf-Clip HolderCOMPACT version
PRODUCTHIGHLIGHTS
NEW:DIVING-PAM-II
Precision Analysis of Photosynthesis
The PAM2500 Portable Chlorophyll Fluorometer succeedsthe renowned PAM2000/2100 instruments which were introduced in the 1990s as the first portable PAM fluorometers.In the development of the PAM2500, particular care was taken to maintain all properties appreciated by PAM2000/2100 users and, at the same time, to integrate stateoftheart technology.
Compared to its predecessors,PAM2500 consistently employs LED technology for saturation pulses, actinic light, single and multiple turnover flashes.
Also, the PAM2500 exhibits amuch improved time resolutiondown to 10 μs and significantlyhigher sensitivity for high qualitymeasurements even with critical samples (e.g. low Chl content).
IMAG-3DChlorophyll Fluorescence System
Compact 3D system based on MAXI Version of the IMAGINGPAM
3D imaging through the highly innovative StripePatternAnalysis by the Fraunhofer Institute for Integrated Circuits IIS
Photosynthetic parameters projected on threedimensional image
Additional morphological parameters for plant phenotyping
Excellently suited for Arabidopsis plants
The IMAG3D system is based on the successful MAXI IMAGINGPAM to which a 3D scanner is added.
Using algorithms developed by the Fraunhofer Institute for Integrated Circuits IIS, the system creates a 3D mesh from images of structured light casted on the plant by a projector.
For 3D imaging, the camera is used in the high resolution mode (1280 x 960 pixels) to precisely digitize the sample surface.
A dedicated tabletop housing offers ideal darkening for 3D and PSII analysis.
Evaluation of leaf morphology
3D image of qN parameter
PAM-2500Portable Chlorophyll Fluorometer
For basic/applied researchand plant screening
Powerful illumination systemfeaturing red, blue, farredlight and single/multiple turnover flashes
Fully computer controlled;optional ultramobile PC fortouch screen operation
Userfriendly and versatilePamWin3 software forquenching analysis, slow/fastkinetic recordings, and lightcurves
New 2030B LeafClip Holderwith a still more accurate PAR sensor
Optional accessories for algae and cyanobacteria
MULTI-COLOR-PAMMultiple Excitation Wavelength Chlorophyll Fluorometer
Unprecedented range ofcolors of measuring andactinic light including whiteand farred
Highly sensitive analysisof dilute chloroplasts or cellsuspensions
Accessory for leaf studiesincluding evaluation ofepidermal UVA screening
Saturation Pulse analysis andfast kinetics recordings with10 μs time resolution
Highly accurate determinationof effective PS II quantum yieldeven under severe lightstress
Determination of functionalPS II absorption crosssectionand PS II turnover rates
Optical unit for leaf measurements
Optical unit for suspensions
The MULTICOLORPAM provides six colors of pulsemodulated measuring light (365 or 400, 440, 480, 540, 590 and625 nm) and five colors of actinic light (440, 480, 540, 590,625) plus white (420640 nm)and farred light (730 nm).
The various colors enableadaptation of excitation light tothe spectral properties of the
sample and to study wavelengthdependent aspects of photosynthesis.
Spherical and flat cosine-corrected sensors are availablefor accurate PARmeasurementsin suspension or at leaf surface, respectively. A special routine is provided for measuring PARlists of all colors.
The GFS3000 is a high precision gas exchange and chlorophyll fluorescence measuring system. The instrument fully controls CO2 and H2O concentrations, cuvette or leaf temperature, ventilation and illumination.
The new LEDArray 3041L provides homogeneous warm white illumination from 1 to ca. 3000 μmol m2 s1 PAR on up to 10 cm2 sample area.
Also, the new LEDArray/PAMFluorometer 3056FL, providinglight control and simultaneouschlorophyll fluorescencemeasurements, excels by itshomogeneity of illumination.
The GFS3000 can be combined with IMAGINGPAM or DUAL PAM100 for simultaneous measurements, analyzing photosynthesis at an elevated level of information.
GFS-3000Portable Gas Exchange & Fluorescence System
Integrated H2O control fordrying and humidifying (0 tonearly 100% rh)
Integrated CO2 control (0 to2000 ppm)
Powerful temperature control
Simultaneous chlorophyll fluorescence measurements
Flexible applications by various cuvette options
GasExchange Chamber 3010GWK1 and LEDPanel RGBWL084 for medium sized samples
Extensive analysis options by combination with other Walzsystems
New: 3041-L and 3056-FL
3010-GWK1 with LED-Panel
DUAL-PAM-100P700 & Chlorophyll Fluorescence System
Simultaneous measurementsof Chl fluorescence and P700 absorbance changes due to innovative dualchannel modulation technique
Fiber version available
Integrated red, blue, andfarred actinic illumination.Saturating single turnover andmultiple turnover flashes
Dedicated DualPAM softwarefeaturing numerous automatedmeasuring routines
Saturation pulse method forsimultaneous assessment ofPS I and PS II quantum yields
Optional emitterdetectorheads for measuring additionalphotosynthetic parameters(ΔpH, NADPH, P515 etc.)
The DUALPAM100 may serve for advanced analysis of photosynthesis parameters in intact leaves and suspensions of algae/cyanobacteria/iso lated chloroplasts. PS I is assessed via dualwavelength P700 measurements (830 vs. 870 nm absorbance) using essentially the same saturation pulse method as for assessment of PS II via Chl fluorescence.
All light sources are integratedin an extremely compactoptical system and controlled via the dedicated DualPAM windows software with 2.5 μs resolution.
Preprogrammed measuringroutines allow complex proto cols to be carried out repro ducibly even by nonexperts.
Measuring setup for suspensions
Leaf holder Laboratory setup
Leaf Clip