Ms Jennifer HuffmanOctober 21, 1997 Ms Jennifer Huffman Wise. Dept. Natural Resources 1125 N....

October 21, 1997

Ms Jennifer HuffmanWise. Dept. Natural Resources1125 N. Military Ave.Green Bay, WI 54307

Re: WET Test (9-16 October, 1997) Results - Lemberger Landfill Site

Dear Jennifer:

Enclosed find the report, and copies of laboratory bench sheets, for the second of the tworequired chronic toxicity retest batteries conducted on treated effluent (groundwater) from theLemberger Landfill Site. Reporting format is the same as that used previously.

The fathead minnow test results were officially inconclusive due to poor survival in the BranchRiver control, but toxicity negative when compared to the secondary control (laboratory water).In contrast to the original and first retest batteries, results for this battery were chronic toxicitynegative for Ceriodaphnia.

Please call if you have any questions or comments.

Sincerely,

Peter J. GlassenProject Manager

enclosurescc Mr. Douglas B. Clark, Foley & Lardner

Mr. Pablo N. Valentin, USEPA Region VMr. David Sanders, RMT

•lairRMT, INC. - Fox VALLEY

RENAISSANCE PLAZA - SUITE 2104351 WEST COLLEGE AVENUE

APPLETON,W! - 54914-3928414/830-0209 - 414/830-1996 FAX

WHOLE EFFLUENT TOXICITY (WET) TEST REPORT FORMGENERAL INFORMATION

FACILITY NAME: LEMBERGER LANDFILL

DISCHARGE DESCRIPTION: TREATED GROUNDWATER

WPDES PERMIT NO: NA

OUTFALL NO.: 001

RECEIVING WATER (RW): BRANCH RIVER

LABORATORY NAME: RMT APPLIED BIOLOGY - 5191

LAB WATER (LW): MHSW

B ABITZ ROAD - APPLETON, WISCONSIN 54915

#2 #3

. - - - . - • • . . - . . • . - . • . • • . • • • • . - • ' . - • . ' • • • . . • • : . . • . ' • • • : • ' • • • • : - . . - : - : • • . • • • :

TEST INFORMATION

DATE TEST INITIATED: 10/9/97

TESTS ARE FOR:

ZID / IWC INFORMATION:

DILUTION WATER:

DILUTION SERIES:

ACUTE - NA

__ WPDES Compliance (required by per_ #1 Retest of failure on / / __

#2 Retest of failure on / /QA Restart from / /Other:

Zone of Initial Dilution Ratio: NA

C. dubia FHM Other:D RW D RW D RWD LW D LW D LW

6.25., 12.5. 25. 50. 100%__ OTHER:

CHRONIC

nit) __ WPDES Compliance (required by permit)#1 Retest of failure on / /

X #2 Retest of failure on 9/4/97QA Restart from / /Other:

Instream Waste Concentration. 100%

C. dubia FHMEl RW El RWD LW D LW

1,3,10,30,100%12.5,25,50,75,100%

X OTHER: 6.25. 12.5. 25. 50. 100%

QA/QC CONDITIONS

ACUTE - NA

TEMPERATURES MAINTAINED DURING TEST? (25+1 "C - CHRONIC)

DISSOLVED OXYGEN >40% AND <100% SATURATION THROUGHOUT TEST?

Effluent pH MAINTAINED WITHIN 6.0 - 9.0 s.u. THROUGHOUT TEST?

REFERENCE TOXICANT TESTS WITHIN ACCEPTABLE LIMITS?

WERE TESTS CONDUCTED IN A 2.5% COj ATMOSPHERE?

COMMENTS:

D YES

D YES

D YES

D YES

D YES

D NO Lx]

D NO E3

D NO B

D NO tE]

D NO D

CHRONIC

YES D NO

YES D NO

YES D NO

YES D NO

YES [x] NO

WATER CHEMISTRY [ALL VALUES REPORTED IN MG/L, EXCEPT pH (s.u.)]

WATER TYPE

RECEIVING

WATER

EFFLUENT

LAB WATER

SAMPLE tt

1

2

3

1

2

3

#1

#2

#3

HARDNESS ALKALINITY TO'

IND

390

390

370

390

80

MEAN IND

390 286

383 308

304

304

MEAN IND

286 <1.4

305 <1.4

<1.4

<1.4

56

FAL NH3-N

MEAN

<L4

<1.4

pH (after warming)

IND MEAN

NA NA

NA NA

T.R. CHLORINE

IND MEAN

<0.01 <0.01

<0.01

<0.01

1. a:\9731RPT.DOC

ACUTE TOXICITY TEST RESULTS i-NA

ZID INFORMATION

SPECIES

FATHEAD MINNOW

AGE OF ORGANISM:

Ceriodaphnia dubla

AGE OF ORGANISM:

<24-h

D. magna

orOTHER:

(Please specify)

AGE OF ORGANISM:

ZID CONCENTRATION: % EFFLUENT

EFFLUENTTREATMENT

LWCONTROL

RWCONTROL

6.25%

12.5%

25%

50%

100%

LWCONTROL

RWCONTROL

6.25%

12.5 %

25%

50%

100%

LWCONTROL

RWCONTROL

6.25%

12.5%

25%

50%

100%

3.3 x ZID CONCENTRATION: % EFFLUENT

PERCENT SURVIVAL BY REPLICATE

1 2

•>

1

3 4

MEANPERCENT SURVIVAL

LW SURVIVAL >90%?

RW SURVIVAL>90%?

LC50:

LW SURVIVAL >90%?

RW SURVIVAL> 90%?

LC50:

LW SURVIVAL >90%?

RW SURVIVAL> 90%?

LC5(I:

D YESD NO

D YESD NO

D YESD NO

D YESD NO

D YESD NO

D YESD NO

COMMENTS:

2. a:\9731RPT.DOC

CHRONIC TOXICITYTEST RESULTS

CONTROLS RECEIVING WATER LABORATORY WATER

FATHEAD MINNOW CERIODAPHNIA DUBIA FATHEAD MINNOW CERIODAPHNIA DUBIA

SURV. >80%? YES NO X YES NO X YES NO X YES NO

MINIMUMPERFORMANCE

>0 25 MG/LARVA?

X YES NO

>15 NEONATES/SURVIVING FEMALE?

X YES ____ NO

>0.25 MG/LARVA?

X YES

>15 NEONATES/SURVIVING FEMALE?

X YES ____ NO

COMMENTS:

LARVAL FATHEAD MINNOW SURVIVAL & GROWTH TEST

EFFLUENTTREATMENT

PROPORTION OF SURVIVAL BYREPLICATE

MEAN PERCENTSURVIVAL

MEAN LARVAL DRY WEIGHT PERREPLICATE

MEAN BIOMASS(MG)

ICJ5

LW CONTROL 10/10 9/10 10/10 10/10 97.5 0.45 0.41 0.39 0.41 0.42 Inconclusive

RW CONTROL 1/10 7/10 10/10 9/10 67.5 0.06 0..24 0.38 0.38 0.27

6.25' 7/10 4/10 6/10 6/10 57.5 0.37 0.19 0.26 0.27 0.27

12.5% 5/10 6/10 4/10 7/10 55 0.28 0.30 0.15 0.37 0.27

25% 6/10 6/10 4/10 10/10 65 0.35 0.33 0.21 0.38 0.32

50% 10/10 7/10 6/10 6/10 72.5 0.39 0.32 0.32 0.34 0.34

100% 10/10 10/10 6/10 10/10 90 0.50 0.44 0.39 0.45 0.45

COMMENTS: " Primary control did not meet minimum survival criterion; IC25 vs secondary control > 100%.

CERIODAPHNIA DUBIA SURVIVAL & REPRODUCTION TEST

EFFLUENTTREATMENT

NEONATE PRODUCTION BY REPLICATE

10

TOTALNEONATESPRODUCED

%SURVIVING

MEANNEONATESPER INITIAL

FEMALE

IC2S

LW CONTROL 41 41 36 42 43 40 40 42 44 41 410 100 41.0 > 100%

RW CONTROL 45 44 42 40 42 38 37 38 40 37 403 100 40.3

6.25 % 45 43 48 38 44 15 42 39 43 42 399 90 39.9

12.5% 47 45 38 38 41 36 36 39 42 44 406 100 40.6

25% 41 42 40 45 38 38 39 40 36 39 398 100 39.8

50% 39 41 40 40 44 42 42 36 43 41 408 100 40.8

100% 37 29 37 36 35 32 37 34 26 31 334 100 33.4

COMMENTS:

3. a.V9731RPTDOC

SAMPLE INFORMATION :

Sample 1

Sample 2

Sample 3

Sample 4

Sample 5

Sample 6

SAMPLETYPE

EFF-24C

EFF-24C

EFF-24C

RIVER-G

SAMPLE COLLECTION

Begin Dale

10/8/97

10/9/97

10/12/97

10/9/97

Begin Time

0630

0700

0630

0730

End Date

10/9/97

10/10/97

10/13/97

NA

End Time

0630

0700

0630

NA

EFFLUENT & RWTEMPERATURE (°C)

Collection

9.0

8.0

9.0

NA

At Lab

3.5

0.4

2.0

7.2

PHat

Lab

8.2

8.5

8.1

7.9

Handdeliver?(If yes, #

hrs.)

Y, S3hr

Y, s2hr

Y, <3hr

Y.i2hr

HoldingTime

<36-h?

YES

YES

YES

YES

YES NO

YES NO

SampleAcceptable?

YES

YES

YES

YES

YES NO

YES NO

DATA SHEET COMPLETED BY:

LABORATORY CONTACT (PLEASE PRINT):

David F. Sanders - Laboratory Director, RMT Applied Biology

PHONE: (920) 830-0209 LAB CERT. NO.: 445142500

PERMITTEE CONTACT (PLEASE PRINT): ,.

Peter J. Glassen, RMT, Inc.

PHONE: (920) 830-0209

SIGNATURE:

DATE: 21 October, 1997

SIGNATURE:

DATE: 21 October, 1997

Send a copy of this form to the following address, according to the timelines specified in your WPDES permit: BiomonitoringCoordinator, Bureau of Watershed Management, Department of Natural Resources, 101 South Webster St., P.O. Box 7921,Madison, Wl 53707-7921

TO BE COMPLETED BY THE DEPARTMENT:

DATE REPORT RECEIVED: RECEIVED WITHIN 45 DAYS?D YES D NO D NA

DID TESTS PASS? ACUTE: C. dubia DYES D NO D Inconclusive/Unacceptable

fathead minnow D YES D NO D Inconclusive/Unacceptable

CHRONIC: C. dubia D YES D NO D Inconclusive/Unacceptable

fathead minnow D YES

RETEST REQUIRED?

RETESTS: ___ ACUTE/CHRONIC

D NO

RESULTS ENTERED INTO DATABASE?

REVIEWED BY:

D Inconclusive/Unacceptable

D YES D NO D NA

DUE TO: D FAILURE D QA/QC PROBLEM

D YES D NO

DATE:

COMMENTS:

CC: AREA ENGINEER/SPECIALIST

PERMIT COORDINATOR

PERMIT FILE

4. a:\9731RPTDOC

Client:

Sampled by:

CHAIN-OF-CUSTODY RECORD

(signature)

INC.

Return Samples to: RMT Applied Biology5191 BAbitz RoadAppleton.Wl54915

SampleIdentification

0 £(

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Carrier Identification (UgSr-Eed Ex, Airborne, ate.) &fOflf<5

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Return Samples to:

INC.

RMT Applied Biology5191BAbitzRoadAppleton.Wl54915


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Carrier Identification (UPS. Fed-Ex, Airborne, etc.)

rWVPFVOM-AQVCHAIN OOC

Project No:

Client:

Organism:

Seven Day Bioassay Chemical Data - Initial

Start Date: I 0 I / 1 "7 "

Ceriodaphnia dubia -Pimephales promelas

Composite #1 Date:Composite #2 Date:Composite #3 Date:

/o /S-/7?7

TestSolution

QC+ 10%(Y/N)

SecondaryControl

(C)ra&Su)-

Param.D.O.PH

Cond.D.O.PH

Cond.Alk.

Hard.SE/B12 (initials)

PrimaryControl

(1)6<*fJcV R,-

AerationD.O.PH

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Hard.Effluent Composite #

c.asvEffluent

(2)ia.r*A>

Effluent(3)

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(4)50 V

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AerationD.O.pH

Cond.

D.O.pH

Cond.D.O.pH

Cond.

D.O.PH

Cond.

D.O.PH

Cond.Alk.

Hard.Determined By:

Exposure Date1

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1\WPFVO\LAB\7DAY1NI.DOC 01/16/97

Seven Day Bioassay Chemical Data - Final

Project No:

Client:

Organism:

TL3 Start Date:

Ceriodaphnia dubia -

TestSolution

QC+ 10%(Y/N)

Secondary<Yi \\SVO -Control

(C)Primary

Oc?(^cK fl-Control

(DOs %'Effluent

(2)

K.$ %'Effluent

(3)

as- %'Effluent

(4)

S6 %'Effluent

(5)

/CO %'Effluent

(6)

Param.D.O.PH

Cond.

D.O.PH

Cond.

D.O.pH

Cond.

D.O.PH

Cond.

D.O.pH

Cond.

D.O.pH

Cond.

D.O.PH

Cond.

D.O.PH

Cond.

Determined By:

Exposure Date1

YV

?,0T1

ff.o?. 4,

^iD

?.6

5.ar.c,

?Q§.4

3,2?'5"

<?>^f.5-

/^

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Y

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t\WPFVOM>B\7DAYCER.DOC 01/18/97

Seven Day Bioassay Chemical Data - Final

Project No:

Client:

Organism:

^73 Start Date: 10

Pimephales promelas

TestSolution

QC+ 10%(Y/N)

SecondaryControl

rnHSiO(C)

Primary6i«(Jcy\ n -

Control(1)

£.3^%-Effluent

(2)

Q.S"°&'Effluent

(3)

2s %'Effluent

(4)SO % 'Effluent

(5)

/tf> °/0 "Effluent

(6)

Param.

D.O.pH

Cond.

D.O.PH

Cond.D.O.

PHCond.

D.O.pH

Cond.

D.O.PH

Cond.

D.O.pH

Cond.D.O.PH

Cond.D.O.pH

Cond.Determined By:

Exposure Date1y

V

7/o1.5-

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(T-^fi^c.-y^•5"

^

1*

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KTC

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YY

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I:\WPFVO\LAB\7DAYPIM.DOC 01/18/97

Ceriodaphnia dubia Survival & Reproduction DataProject No:

Start Date:

Start Time:

Set by:Age/Source:

Feeding By:

tn3\ -10 /<! If-? x

IbSto -ATC /" <3o<tfJ -*•

^Kr -A/ 1-3^-17• j *JO fr~u—^ JOd1 (£v_ d2 (CJ*— "3

Client:Finish Date:

Finish Time:

% 3rd Brood:Test Endpoint:

>f d4 X

Le.«Y\b£.rqe.r "10/15^1 ^ -I05o " "Day 6 /oo0/^3^7

(Day ff} ^ • Day 7

d5 flTL, d6 )r d7

Day 8Day 8

d8

Test

Solution

Secondary

Control

(C)

jv\HSU3 ^

Rep.

1

2

3

4

5

6

7

8

9

10

Determined By:

Neonates/Exposure Day

1

el<

<

a "6

2

6/e

>f

3

S1t>7s7s ••tbZ\t

4

|3^\t|e^1^g£

££^

5

I

13£

/S(H615il\k>13*f

6

20

IIIt.

zoZl

^1202122.2.0

JP

7 8

Comments:

Test

Solution

&c<*tocV\ ^

Primary

Control

d)

Rep.

12

3

4

5

6

7

8

9

10

Determined By:

Neonates/Exposure Day1

okt

1

(t£

2

fr/£'

>?

3

sT1-]T-j(9

(o<0

>P

4

|L|

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5

E(5"E£IM

1110

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11>f

6

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7 8

Comments:

Endpoint: Day^/B

Total

Neonates

Ml -"Ml --3k --H3 --S3 -10 --^o -^H^ --H^i --Hi -'

Total Neonates:

Total

Broods

3 .

3 ,

3 -J ,3 '3 •3 ,3 x3 ,3 -

%3fd Brood:

)oo -^

Mortality

K Survival:

|cjO .

Initial Adults: JO ' -•Neonates/lnitial Adult: Hl-0 * -^Surviving Adults: JO '_ -Neonales/Surviving Adult: Hl-° " ^

Endpoint: Da>C0/7/8

Total

Neonates

4S~ '.V1/ v

* ot "^L( /^^\ —

*U '-3Sr -3-7 -.3^ -^fo v37 v

Total Neonates:

Total

Broods

3 -3 -3 >3 .3 ,3 -3 .3 -3 '3 -

%3rd Brood:

(00 -'

Mortality

K Survival:

(DO' **

Initial Adults: |0 ' -Neonates/lnitial Adult: 4^-3Surviving Adults: (0 - •" ^ ____Neonates/Surviving Adult: nOi^ --

G = Gravid G/E = Gravid/Eyed E=Eyed NA- No Animal AD = Adult DeadYD = Young Dead ABY = Aborted Young M= Male K = Killed in Transfer Date Check: >P

PS,

I:\WPFVOVLAB\CERIO.DOC

Ceriodaphnia dubia Survival & Reproduction Data

N o : " C l i e n t :

Day 7

Start Date: \0 1 ^ |

Test Endpoint: Day 6 Day 8

TestSolution

c,ar%'Effluent

(2)

Rep.

123

4

5

6

7

8

9

10

Determined By:


olxi

.

<ftc

2

fr/t

^r

3

$

1fc>

57

••*>b't>t>i>p

4

iHÎ*»a^pf£*^/i

5

£/Z££14

9/*DliIIIM13>f

6

i32M

ZH2.123

232 1^322.

df

7 8

Comments:

Endpoint: Day(^7/8Total

Neonates

fr -

V^> --vr -38T -4V —/sr -"va -3^ v-^^ -'H^ -

Total

Broods

33 -3 ,3 -3 -a -? -3 -3 -3 -

Mortality

•S it*

TOTAL % SURVIVAL:NEONATES: ^-y\\/ ô-INfTIAL ADULTS: NEONATES/ADULT:

ID' 3<t-V-

TestSolution

\3 .S%

Effluent

(3)

Rep.

12

3

4

5

6

7

8

9

10

Determined By:


<k\

.->

to

2

fr/E

»f

3

SL>

k><i>

(o5

<o(011

JP

4

l*f

1"?0IIJ5^L^/^â^

5

B£12.e13E/ IJ3.1115^P

6

-iHll10

2. \IL20

^20Z\2Z

>r

7 8

Comments:

Endpoint: Day@j7/8Total

Neonates

M7 --Vr -s

1>% -~3t •*HI -~34 ".u --31 •-^ -.^ -

TotalBroods

7-3 .3 -3 ,3 -3 -3 -3 ~3 -3.

Mortality

TOTAL % SURVIVAL:NEONATES: ^

Holo"^ '°°'INmAL ADULTS: NEONATES/ADULT:

fC? - t(6,t '

G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male

AD = Adult DeadK = Killed in Transfer Date Check:

I:\WPFVCALAB\CERIO2.DOC


Project No: ^"73 | - ______ Client: q

Start Date: )C / ^ j *?*7 •"

Test Endpoint: Day 6 ^/' Day 7 Day8

TestSolution

as-%'Effluent

(4)

Rep.1

2

3

45

6

7

8

9

10

Determined By:


r>k

,

«?•

2

It**

>9

3

&

b\o3kk> .7fc>feV

>P

4

13£

12IS^11^(310|3flr^

5

£|(PEeKE12.£££^f

6

,2120

22.

^321

2120

i\ib10

>P

7 8

Comments:

Endpoint: Da/^7/8

TotalNeonates

HI -Ha --«/0 '-vr --JS^ -,3S -3<1 --Ho ^34 -3^ „

Total

Broods

? .3 -3 -^ -3 -3 -3 '3 -> -3 -

Mortality

TOTAL % SURVIVALNEONATES:

3?8-^ /CO:INmAL ADULTS: NEONATES/ADULT-

/O'- J?.?'

TestSolution

So %'Effluent

(5)

Rep.1

23

4

5

6

7

8

9

10

Determined By:


rite

.IW-

2

6/6

>p

3

5(pkb111t>•7-7it

A

(3^iHrt£iH£I]gftfac^

5

6

Wt

£14£13E«*e»r

6

^1

-il20

Z J23il22

'^^322.

*f

7 8

Comments:

Endpoint: Day®7/B

Total

Neonates

31 xLl( -x

Mt> -VQ-^ -HJ -^^ -.Jt-M3 ^M( ^

Total

Broods

3 -3 -3 -3 -3 -33 -3 '3 -3 -

Mortality

TOTAL % SURVIVAL:NEONATES: / /oCX _- X40* ^- )0oINmAL ADULTS: NEONATES/ADULT:

/o ip.fr ^G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male

AD = Adult DeadK - Killed in Transfer Date Check:

I:\WPFVO\LAB\CERIO2.DOC

project NO:


C|ient:

Start Date: |Q / ^ /*?"? "

Test Endpoint: Day 6 Day? Day 8

Test

Solution

100%"Effluent

(6)

Rep.1

2

3

4

5

6

7

8

9

10

Determined By:


<S\<

•

(fi*-

2

frfc

1

^P

3

87bbfcIff •1t>55>?

4

Mr?tttt£tE£to

5

£Sli'3(111MII512.><>

6

1$

HIS'1(S»51°)ni<*IH>r

7 8

Comments:

Endpoint: Day@7/8

Total

Neonates

37 vai •-37 o?4 -35^ '-^J3--37 --3V -,34 '.31 x.

TotalBroods

3*-3 .1 -3 -3 -3 -3 -3 ,3 '3 -

Mortality

TOTAL % SURVIVAL:NEONATES: -j 5 (_/

3J( ' /(5t> '-INITIAL ADULTS: NEONATES/ADULT:

/O S 33-M -^

Test

Solution

Effluent

( )

Rep.1

2

3

4

5

6

7

8

g10

Determined By:

Neonates/Exposure Day1 2 3 4 5 6 7 8

Comments:

Endpoint: Day 6/7/8

Total

NeonatesTotal

Broods Mortality

TOTAL * SURVIVAL'NEONATES:

INITIAL ADULTS: NEONATES/ADULT

G = Gravid G/E = Gravid/Eyed E=Eyed NA = No AnimalYD = Young Dead ABY = Aborted Young M= Male

AD = Adult DeadK = Killed in Transfer Date Check:

I:\WPFVO\LAB\CERI02.DOC

Pimephales promelas Survival & Growth Data

Project No:

Start Date:

Start Time:l<=l /

Client:Finish Date:

Finish Time:

Set by: «7 C Age: < 24 hour

Test

Solution Rep.

Fatalities / Survivors Per Day

6

Total

Fatal. Surv. Surv.Mean

Surv.

SecondaryControl

(C)

.* o IOOVi

(0D [0

PrimaryControl

d)

0//CJ */!!•

"/l o '/I 0/7 3Via O 10

fill* /c

(o70

%'Effluent

(2)

olio Vii* 70/c| Y

D

70 -57,5

cllo Vs o/S" 5Effluent

(3)

OllO •/*Olio

D olio 0/7 3 7

5*^-s

7C?2 5 %Effluent

(4)

OHO 3/1V(0

olioVlO "/it?

HoIOO

So %Effluent

(5)

Oho o•M* */? 3

D

(oo -70

loo °/0 -Effluent

(6)

Olio •/I. "/To Vtto 0 10Olio 10

oht> Mo V-7 0/7 Moho 10

(oO -

/£*>Determined By:

Comments:

Data Check:

I:\WPFVO\LAB\P1MEPHAL.DOC

Sfu't'

Larval Fathead Minnow Weight

ar ft^VReflg o.«*<i<i<i<i / Kooooa

^ Ref 5g 5*^000 i ' 5i 00003•.«•

/Test

Solutionc.

SecondaryControlMHSW •-

1PrimaryControl

3rantk ft • "

<,•«*<» -Effluent

13)

N.S% -Eflluent

^3)

25*. -Effluent

W /

So*?* -Effluent

C^^)00?0 '

Effluent

(£)Effluent

Rep12341234123412341234123412341234

PanID

ClClt3£MtA/ a1C

101A2.6XC2-0M36ic304A46«4tHO945(b5CSOfc/|fafttc/00

TARE +*^ Sample fe) */•aiwt -y ^/. ^00^1/•:?oato ^ ^/.ayoo^(.30033 '[,m\W1 . 375^ (/.36613L/. s^car/.il1/^/.3?CS"7/. ?65£ 3/.annvH/.as'y^D/. A1830/..lOM?/. 50 /yj/. 3=10%/. 31V5"!f.SS'VO^/.afiT35t/. afcoJ^l/ .a7?(// . 306/31- ~*>0%13.h a.-^ooir/• 97 //a1-30230

!»/<$• /o// t

? TARE (g)1- 2-7M151. ilfrTH1, 2°l^l II- 2.lS<nI.'ZI^TSI. 2<plQM

1. 271 le«>1. 30290

1. 2<USt,i, z<uiq1. 28H2Ti. 5oia<*i. a'JfciSi. i8 SSS

1. 2TfcTl1- 2^751i, ?.<ri<n. 287W

). 2*?l3q1.280331. 27^2.i. 2571^. 2T*<* 2.

( • 3o lTJi .2qnq/». USTo1. 2(»12)1- 2<n$'S

# Fish ^Biomass

/o

n

s

# Fish __Mn wtft>7

A>/o

/7

/O7

I \WPFVCHLAB\FSHWTOAT DOC

Project

Test start

9731

10/15,16/97

Balance Calibration:

TestSolution

SecondaryControl

C

PrimaryControl

1Branch R.

6.25%2

12.50%3

25%4

50%5

100%6

RepABCDABCDABCDABCDABCDABCDABCD

PanIDC1C2C3C41A1B1C1D2A2B2C2D3A3B3C3D4A4B4C4D5A5B5C5D6A6B6C6D

Client

Initials

Yes

Tare +Sample (g)

1.27866 -1.30081 -1.30260 -1.28009 -1.30033 -1.27148 -1.27541 -1.30672-1.29625 -1.29466 -1.28687 -1.30563 -1.29914 -1.28860 -1.29820 *1.30118 <-1.30143 .1.29046 -1.29451 >•1.28408 -1.28351 -1.26039 -1.27811 ^1.30613 -1.30292 .1.30015 -1.27112 x1.30230 ^

Larval Fathead Minnow Weight Data

Lemberger Landfill

TP, RJC

X

Tare (g)1.27413-1.29674 -1.29871 '1.27597-1.29975 -1.26904 -1.27160 -1.30290 -1.29256 .1.29279-1.28427 '1.30289 -1.29635 .1.28558-1.29671 ,1.29751 -1.29797 -1.28714 -1.29239 s1.28033 -1.27962 -1.25716-1.27492 -1.30271 -1.29791 -1.29570 x1.26721 '1. 29783 /-

No

Sample (mg)4.534.073.894.120.582.443.813.823.691.872.6

2.742.793.021.493.673.463.322.123.753.893.233.193.425.014.453.914.47

Biomass# of Fish @ Start

10101010101010101010101010101010101010101010101010101010

Weight (mg)0.450.410.390.410.06 "*0.240.380.380.370.190.260.270.280.300.150.370.350.330.210.38 f0.390.320.320.340.500.4410.390.45

Reference Wgts:

Nominal1.000005.00000

Actual0.99999/1 .000025.00001/5.00003

Mean Wgt.# of Fish @ Term.

109101017109

Weight (mg)0.450.45 -0.390.410.58 .0.35 .0.380.42 "

#DIV/0!#DIV/0!#DIV/OI#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!#DIV/0!

MeanBiomass

0.42

0.27

0.27

0.27

0.32

0.34

0.45

MeanWeight

0.43

0.43

#DIV/0!

#DIV/0!

#DIV/0!

#DIV/0!

#DIV/0!

•^ "" /

a:\9731fwc.XLS

Cone. ID

Cone. Tested

ResponseResponseResponseResponseResponseResponseResponseResponseResponseResponse

12345678910

1

0

45-44-42-40-42-38-37-38-40-37,

' 2

6.25

45 -43'48'38'44-15'42'39-43-42-

3

12.5

47-45'38'38'41'36'36'39-42-44-

4

25

41'42-40'45-38'38'39-40.36-39-

5

50

39-41"40'40'44'42-42'36-43-41-

' 6

100

37293736353237342631

*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Landfill "—Test Start Date: 10/9/97 ' Test Ending Date: 10/15/97-_Test Species: Ceriodaphnia dubia 'Test Duration: 6-days 1DATA FILE:

Cone.ID

123456

NumberReplicates

101010101010

Concentration% Effluent

0.6.12.25.50.

100.

000250500000000000

ResponseMeans

40.39.40.39.40.33.

300 *•900"600^800"800-400-

Std. PooledDev. Response Means

293223

.869

.195

.836

.486

.251

.806

40.40.40.40.40.33.

300275275275275400

*** No Linear Interpolation Estimate can be calculated from the *-input data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean. *

,/,./•<

1 ;> I •"';>

Cone. ID

Cone. Tested

ResponseResponseResponseResponse

1234

0

0.06-0.24-0.38-0.38-

2 '

6.25

0.37-0.19-0.26-0.27-

3

12.5

0.28 '0.30'0.15-0.37-

4 ^

25

0.35-0.33'0.21-0.38-

5 ^

50

0.39-0.32-0.32'0.34-

6

100

0.500.440.390.45

*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Effluent "Test Start Date: 10/9/97 ' Test Ending Date: 10/16/97 -Test Species: Pimephales promelas 'Test Duration: 7-days "DATA FILE:

Cone . NumberID Replicates

123456

444444


0.0006.25012.5'bO25.00050.000100.000

ResponseMeans

0.265-0.273-0.275-0.318'0.343'0.445-


0.1520.0740.0920.0750.0330.045

0.3200.3200.3200.3200.3200.320

*** No Linear Interpolation Estimate can be calculated from theinput data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean.

P,

Cone. ID

Cone. Tested

ResponseResponseResponseResponse

1234

1

0

0.45-0.41-0.39-0.41-

2 ^

6.25

0.37-0.19-0.26.0.27.

3 "

12.5

0.28-0.30-0.15-0.37-

4

25

0.35-0.33-0.21'0.38-

5 s

50

0.39-0.32-0.32-0.34'

6

100

0.500.440.390.45

*** Inhibition Concentration Percentage Estimate ***Toxicant/Effluent: Lemberger Effluent'Test Start Date: 10/9/97 - Test Ending Date: 10/16/97-Test Species: Pimephales promelas'Test Duration: 7-days 'DATA FILE:

Cone.ID

123456

NumberReplicates

444444


0.0006.25012.50025.00050.000100.000

ResponseMeans

0.4150.2730.2750.3180.3430.445


0.0250.0740.0920.0750.0330.045

0.4150.3310.3310.3310.3310.331

*** No Linear Interpolation Estimate can be calculated from theinput data since none of the (possibly pooled) group response meanswere less than 75% of the control response mean.

±L- I ff° rr>ei'e"f "i-A _ /—•———~

Sanders, Dave F

From:To:Subject:Date:

Palafox, Dennis (Sidney)Sanders, Dave FSediment Quality CriteriaMonday, October 20, 1997 6: 17PM

I need some help with determining appropriate SQCs for an ecological risk assessment. As you know,SQCs are calculated according to the following equation:

SQC = foc*Koc*FCV

where: foe = mass fraction of organic carbon for sedimentKoc = organic carbon partition coefficientFCV = final chronic value, from chronic ambient water quality criteria

Sediment values are normalized to 1 % organic carbon. My question is -- can I recalculate a new SQC if myTOC values are greater than 1 %? If so, can I substitute the TOC percentage directly into the equation forfoe or do I have to is-4here some type of manipulation before I can use it. What do you think?

-^ •>Thanks,

Dennis

.01

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