(MRC)2MetabolomicsWorkshopMay9-12,2016

WelcometotheMichiganRegionalComprehensiveMetabolomicsResourceCore(MRC)22016MetabolomicsWorkshop.

Location(Day1-3):KelloggEyeCenter

OliphantMarshallAuditoriumUniversityofMichigan1000WallStreet

AnnArbor,MI48105

Location(Day4):TaubmanHealthSciencesLibraryRooms5215/5219(ComputerLabs)

UniversityofMichigan1135CatherineSt,AnnArbor,MI48109

Workshopgoal:Tobuildasolidfoundationforparticipantstoexpandtheirresearchusingmetabolomics.Aim1: Tounderstandtheprinciplesoftheanalyticalmethodologies anddataanalysistechniquesusedinmetabolomics.Aim2: Tousethisknowledgetoincorporatemetabolomicsintoyour research.Aim3: Tounderstandthereasoningbehinddataprocessingand statisticalanalysis.Aim4: Togainaworkingfamiliaritywithbioinformaticstoolsfor

exploratoryanalysis.ThisworkshopissupportedbygrantU24DK097153ofNIHCommonFundsProjecttotheUniversityofMichigan.

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~AGENDA~Monday,May9–Day18:00a.m. Registration,coffeeandlightbreakfast KelloggEyeCenterlobbyMorningSessionsMetabolomicsoverview.Whattoconsiderwhenyouaredesigningyourexperiment?9:00a.m. Welcome AllaKarnovsky9:15a.m. IntroductiontoMetabolomicsinResearch CharlesBurant9:35a.m. OverviewandExamplesofMetabolomics

Methods:Targeted,UntargetedandFluxomics CharlesEvans10:00a.m. ExperimentalDesign: SpecialConsiderationsforMetabolomics AnnaMathew10:30a.m. Break 10:45a.m. SampleCollection:GuidelinesandCautions KelliSas11:15a.m. CasesfromtheCore:EmphasisonStudyDesign andSampleCollection CharlesBurant11:45a.m. Lunch KelloggEyeCenterlobby

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AfternoonSessionsMetabolomicsinstrumentation,NMRmetabolomics.1:00p.m. MetabolomicsInstrumentation:Mass

SpectrometryandOtherPlatforms CharlesEvans1:45p.m. ChromatographicSeparationsinMetabolomics:

Why,What,How? StefanieWernisch 2:15p.m. InteractingwiththeCore MaureenKachman2:45p.m. Break 3:00p.m. CaseStudy:MethodofAnesthesiaAffects MetabolomicsofMammalianTissues CharlesEvans 3:30p.m. NuclearMagneticResonance(NMR)

Metabolomics KathleenStringer 4:00p.m. TranslationalResearchinDiabeticKidney

Disease(DKD) SubramaniamPennathur

4:30p.m. Q&Awithspeakersfromtheday4:45p.m. MetabolomicsinBiomedicalResearch:ALast

ResortortheNextFrontier? GaryPatti,WashingtonUniversity5:45p.m. Reception KelloggEyeCenterlobby

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Tuesday,May10–Day28:00a.m. Coffeeserviceandlightbreakfast KelloggEyeCenterlobbyMorningSessionsTargetedAnalysis;Introductiontostatisticalanalysisformetabolomics9:00a.m. DataAnalysisforTargetedMetabolomicsStudies MaureenKachman9:20a.m. PUFACaseStudyonTargetedMetabolomics CharlesBurant9:50a.m. Break 10:00a.m. IntroductiontoStatistics ShervinAssari11:00a.m. AnalysisoftheTargetedDataSet CharlesBurant11:45a.m. Lunch KelloggEyeCenterlobbyAfternoonSessionsFluxomics;Lipidomics1:00p.m. ApplicationofStableIsotopeLabelingin MetabolomicsandFluxomics MahmoudElAzzouny1:45p.m. DataAnalysiswithVistaFlux GarrisonBirch,AgilentTechnologies

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2:30p.m. Break2:45p.m. IntroductiontoLipidBiology CharlesBurant3:00p.m. UntargetedRP-UPLC-QTOF-MS/MSBased

Lipidomics:AGlobalLipidomicProfilingofComplexBiologicalSamples

Thekkelnaycke"TM"Rajendiran 3:30p.m. LipidomicsDataAnalysisandReport TanuSoni4:00p.m. CaseSeries:LipidProfiling FarsadAfshinnia 4:30p.m. Q&Awithspeakersfromtheday4:45p.m. MetabolomicsCoreLabTour(Q&A) GatherintheKelloggEyeCenterlobby Wednesday,May11–Day38:00a.m. Coffeeandlightbreakfast KelloggEyeCenterlobbyMorningSessionsUntargetedmetabolomics;Advancedstatisticalanalysis. 9:00a.m. UntargetedDataAnalysisWorkflowand

RecursiveAnalysisforUntargetedMetabolomics MaureenKachman9:45a.m. MetabolomicsRevealDysregulationofLinoleic

AcidMetabolisminBipolarDisorderSimonEvans

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10:15a.m. AdvancedStatisticalMethodsI GeorgeMichailidis11:15a.m. Break11:30a.m. AdvancedStatisticalMethodsII GeorgeMichailidis12:15p.m. Lunch KelloggEyeCenterlobbyAfternoonSessionsBioinformaticsanalysis:pathwaysandbeyond;IntegrationwithotherOmics.1:15p.m. ToolsforBioinformaticsAnalysis,Visualization,

andDataIntegration AllaKarnovsky1:30p.m. MetaboliteSetEnrichmentAnalysisand

MetabolicPathwayAnalysis Jianguo“Jeff”Xia,McGillUniversity2:00p.m. MummichogandApplications ShuzhaoLi,EmoryUniversity2:30p.m. Data-drivenApproachesandMulti-omics

Integration GeorgeMichailidis3:00p.m. Break 3:15p.m. MetabolomicsDataProcessingand StatisticalAnalysis Jianguo“Jeff”Xia,McGillUniversity

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3:45p.m. Hands-onPreview:CoolMap CharlesBurant4:00p.m. Hands-onPreviewandExercise:Statistics TanuSoni4:30p.m. Hands-onPreview:MetScape AllaKarnovsky5:00p.m. Q&Awithspeakersfromtheday Thursday,May12–Day48:00a.m. Coffeeserviceandlightbreakfast KelloggEyeCenterlobbyMorningSessionsHands-onSessionsatTaubmanHealthSciencesLibrary9:00a.m. StatisticalWorkflows TanuSoni Room5215/19THSL12:00p.m. Lunch(Room5000)AfternoonSessions 1:00p.m. ConcurrentSessionI (1a)MetScape(PathwayAnalysis) MarciBrandenburg,BillDurenandAllaKarnovsky Room5215 (1b)CoolMap FanMeng Room5219

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3:00p.m. ConcurrentsessionII: (2a)CoolMap FanMeng Room5219 (2b)MetScape(PathwayAnalysis) MarciBrandenburg,BillDurenandAllaKarnovsky Room52155:00p.m. WorkshopConcludes Thankyouforjoiningus!

DirectoryofPresentersName Email Institution

Assari,Shervin assari@umich.edu UniversityofMichiganAfshinnia,Farsad fafshin@med.umich.edu UniversityofMichiganBirch,Garrison garrison.birch@agilent.com AgilentTechnologies

Brandenburg,Marci mbradenb@umich.edu UniversityofMichiganBurant,Charles burantc@umich.edu UniversityofMichiganDuren,Bill wld@umich.edu UniversityofMichiganEvans,Charles chevans@umich.edu UniversityofMichiganEvans,Simon evanssi@umich.edu UniversityofMichiganElAzzouny,Mahmoud

mazzouny@umich.edu UniversityofMichigan

Kachman,Maureen mkachman@umich.edu UniversityofMichiganKarnovsky,Alla akarnovs@umich.edu UniversityofMichiganLi,Shuzhao sli49@emory.edu EmoryUniversityMathew,Anna amat@umich.edu UniversityofMichiganMeng,Fan mengf@umich.edu UniversityofMichiganMichailidis,George gmichail@umich.edu UniversityofFloridaPatti,Gary gjpattij@wustl.edu WashingtonUniversityin

St.LouisPennathur,Subramaniam"Sub"

spennath@umich.edu UniversityofMichigan

Rajendiran,Thekkelnaycke"TM"

tmraj@umich.edu UniversityofMichigan

Sas,Kelli sask@umich.edu UniversityofMichiganSoni,Tanu tanusoni@umich.edu UniversityofMichiganStringer,Kathleen stringek@umich.edu UniversityofMichiganWernisch,Stefanie wernisch@umich.edu UniversityofMichiganXia,Jianguo“Jeff” jeff.xia@mcgill.ca McGillUniversity

MRC2(MetabolomicsCore)Team

Fromlefttoright:Frontrow:TerriRidenour,CharlesEvans,SubPennathur,TanuSoni,ChuckBurant,KariBondsBackrow:TMRajendiran,ChunhaiRuan,BillDuren,JanWigginton,RobertChomic,JaemanByun,LiZhang,ArunDas,SashaRaskind,JaneCao,MaureenKachman,DongyuWang,HeidiBaumNotpictured:GraceWu,AllaKarnovsky