MOUSAM RANJAN DASH ON TERMINATOR TECHNOLOGY

8/9/2019 MOUSAM RANJAN DASH ON TERMINATOR TECHNOLOGY

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Under theUnder theMR. NILADMR. NILADSCHOOL OF BIOTECHNSCHOOL OF BIOTECHN

By MousamBy MousamBSc.BSc.

Roll .8Roll .8

u

idance ofuidance ofRI BHUSANRI BHUSAN

LOGY,KIIT UNIVERSITYLOGY,KIIT UNIVERSITY

Ranjan DashRanjan DashLLBLLB8400684006


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ermin or echnolo i n emerging ield oermin or echnology i n emerging ield oeneticengineering u ed b ically bytheMNCeneticengineering u ed basically bytheMNCsommercial purpose.ommercial purpose.

his isa verycontroversial technology. t is beinghis isa verycontroversial technology. t is beingsed byMNCs producinggeneticallymodi iedsed byMNCs producinggeneticallymodi iedeeds, likeMON ANTO.eeds, likeMON ANTO.

t producessterileseedsand makesthe poort producessterileseedsand makesthe poorarmers dependentastheseseedsareto be bougarmers dependentastheseseedsareto be bougnnuallyafter harvesting.nnuallyafter harvesting.

erminatortechnology is

an emergingfield oferminatortechnology isan emergingfield ofeneticengineering used basically bytheMNCseneticengineering used basically bytheMNCsommercial purpose.ommercial purpose.

his isa verycontroversial technology.It is beinghis isa verycontroversial technology.It is beingsed byMNCs producinggeneticallymodifiedsed byMNCs producinggeneticallymodifiedeeds, likeMON ANTO.eeds, likeMON ANTO.

t producessterileseedsand makesthe poort producessterileseedsand makesthe poorarmers dependentastheseseedsareto be bougarmers dependentastheseseedsareto be bougnnuallyafter harvesting.nnuallyafter harvesting.


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his is in contrastto traditional way offarminghis is in contrastto traditional way offarmingherethefarmers used to storesomeseedsfroherethefarmers used to storesomeseedsfro

heir harvestso thattheycould use it in theheir harvestso thattheycould use it in theollowingyear.ollowingyear.

ut bythe introduction ofterminatortechnologyut bythe introduction ofterminatortechnologys now possiblefortheMNCsto producesuchs now possiblefortheMNCsto producesucheedswhich aresterileand astheyare biologicalleedswhich aresterileand astheyare biologicallnd geneticallymodified.nd geneticallymodified.

his is in contrastto tradi

tional way offarminghis is in contrastto traditional way offarmingherethefarmers used to storesomeseedsfroherethefarmers used to storesomeseedsfro

heir harvestso thattheycould use it in theheir harvestso thattheycould use it in theollowingyear.ollowingyear.

ut bythe introduction ofterminatortechnologyut bythe introduction ofterminatortechnologys now possiblefortheMNCsto producesuchs now possiblefortheMNCsto producesucheedswhich aresterileand astheyare biologicalleedswhich aresterileand astheyare biologicallnd geneticallymodified.nd geneticallymodified.


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o wecan now saythatthistechnology is designo wecan now saythatthistechnology is designo asto make biotechnologyo asto make biotechnology--derived plantsderived plantsroducing nonroducing non--viableseeds.This preventstheviableseeds.This preventsthearmersfrom savingseedsand replantingthem.armersfrom savingseedsand replantingthem.

he positiveaspectaboutthistechnology isthat ihe positiveaspectaboutthistechnology isthat ireventsescape ofreventsescape ofTransgenesTransgenes into theinto thenvironment.nvironment.

o wecan now saythatt

histechnology is designo wecan now saythatthistechnology is designo asto make biotechnologyo asto make biotechnology--derived plantsderived plantsroducing nonroducing non--viableseeds.This preventstheviableseeds.This preventsthearmersfrom savingseedsand replantingthem.armersfrom savingseedsand replantingthem.

he positiveaspectaboutthistechnology isthat ihe positiveaspectaboutthistechnology isthat ireventsescape ofreventsescape ofTransgenesTransgenes into theinto thenvironment.nvironment.


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ow lets know how thetechnologyworksactuallow lets know how thetechnologyworksactuallroducing nonroducing non--viableseeds.viableseeds.

orthis, I havechoseto giveaschematicandorthis, I havechoseto giveaschematicandiagrammatic way ofpresentation.iagrammatic way ofpresentation.

irst, we will go through the normal way, how airst, we will go through the normal way, how a

erminator works?takingthecase ofcotton planterminator works?takingthecase ofcotton plantnd then followed by introducinga inducerandnd then followed by introducinga inducerandhatarethechanges dueto this inducer?hatarethechanges dueto this inducer?

ow lets know how thete

chnologyworksactuallow lets know how thetechnologyworksactuallroducing nonroducing non--viableseeds.viableseeds.

orthis, I havechoseto giveaschematicandorthis, I havechoseto giveaschematicandiagrammatic way ofpresentation.iagrammatic way ofpresentation.

irst, we will go through the normal way, how airst, we will go through the normal way, how a

erminator works?takingthecase ofcotton planterminator works?takingthecase ofcotton plantnd then followed by introducinga inducerandnd then followed by introducinga inducerandhatarethechanges dueto this inducer?hatarethechanges dueto this inducer?


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I isarepressorgenethatproducesarepressorproteinthat interacts itha indiI isarepressorgenethatproducesarepressorproteinthat interacts itha indi

eneII.eneII.

II isarecombinasegenethat iscontrolledbyapromoter. etweenthegeneandII isarecombinasegenethat iscontrolledbyapromoter. etweenthegeneand

oter isabindingsitefortherepressorfrom eneI.Therecombinasegeneproduoter isabindingsitefortherepressorfrom eneI.Therecombinasegeneprodubinaseproteinthat isanenzymeandsnipsoutpiecesof .binaseproteinthat isanenzymeandsnipsoutpiecesof .

IIIproducesatoxinthat is lethal toembryos.Thegene iscontrolledbya lateproIIIproducesatoxinthat is lethal toembryos.Thegene iscontrolledbya latepro

isactiveonlyduringthe latestageofseeddevelopmentwhentheembryo isdeisactiveonlyduringthe latestageofseeddevelopmentwhentheembryo isde

eenthe latepromoterandthetoxingene isapieceof calledablocker, whiceenthe latepromoterandthetoxingene isapieceof calledablocker, whicereswiththeabilityofthepromotertoturnonthetoxingene.ereswiththeabilityofthepromotertoturnonthetoxingene.

nducer isachemical appliedtotheseedbytheseedcompanythatwill initiatethnducer isachemical appliedtotheseedbytheseedcompanythatwill initiateth

inatorgene interactions.inatorgene interactions.

I isarepressorgenethatproducesarep

ressorproteinthat interactswithabindiI isarepressorgenethatproducesarepressorproteinthat interactswithabindi

eneII.eneII.

II isarecombinasegenethat iscontrolledbyapromoter. etweenthegeneandII isarecombinasegenethat iscontrolledbyapromoter. etweenthegeneand

oter isabindingsitefortherepressorfrom eneI.Therecombinasegeneproduoter isabindingsitefortherepressorfrom eneI.Therecombinasegeneprodubinaseproteinthat isanenzymeandsnipsoutpiecesof .binaseproteinthat isanenzymeandsnipsoutpiecesof .

IIIproducesatoxinthat is lethal toembryos.Thegene iscontrolledbya lateproIIIproducesatoxinthat is lethal toembryos.Thegene iscontrolledbya latepro

isactiveonlyduringthe latestageofseeddevelopmentwhentheembryo isdeisactiveonlyduringthe latestageofseeddevelopmentwhentheembryo isde

eenthe latepromoterandthetoxingene isapieceof calledablocker, whiceenthe latepromoterandthetoxingene isapieceof calledablocker, whicereswiththeabilityofthepromotertoturnonthetoxingene.ereswiththeabilityofthepromotertoturnonthetoxingene.

nducer isachemical appliedtotheseedbytheseedcompanythatwill initiatethnducer isachemical appliedtotheseedbytheseedcompanythatwill initiateth

inatorgene interactions.inatorgene interactions.


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Patent for Terminator GenePatent for Terminator GeneControl of plant gene expressionControl of plant gene expression

Patent #5,723,765 Oliver ,Patent #5,723,765 Oliver ,et al./Delta Pine Land Co. US Patent Office 3rdmarch1998.et al./Delta Pine Land Co. US Patent Office 3rdmarch1998.

patentunder ontrol ofgeneexpressionhaspatentunder ontrol ofgeneexpressionhaseengranted.eengranted.

heysuccessfullygetpatentedbytheirstrongheysuccessfullygetpatentedbytheirstrong

laimswhichwereabout 55 innumbersandthelaimswhichwereabout 55 innumbersandthe55thth states, 55.Amethodofproducingnonstates, 55.Amethodofproducingnon--viablviableedeed

Patent for Terminator GenePatent for Terminator GeneControl of plant gene expressionControl of plant gene expression

Patent #5,723,765 Oliver ,Patent #5,723,765 Oliver ,et al./Delta Pine Land Co. US Patent Office 3rdmarch1998.et al./Delta Pine Land Co. US Patent Office 3rdmarch1998.

patentunder ontrol ofgeneexpressionhaspatentunder ontrol ofgeneexpressionhaseengranted.eengranted.

heysuccessfullygetpatentedbytheirstrongheysuccessfullygetpatentedbytheirstrong

laimswhichwereabout 55 innumbersandthelaimswhichwereabout 55 innumbersandthe55thth states, 55.Amethodofproducingnonstates, 55.Amethodofproducingnon--viablviableedeed


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Geneticallymodifiedfoodaremadesoasto lookGeneticallymodifiedfoodaremadesoasto lookafterthescarcityoffoodandresources, itsafterthescarcityoffoodandresources, its

objectivewasnevermeanttocreateanythingthobjectivewasnevermeanttocreateanythingthwouldbefatal toanyraceoranybioticorabioticwouldbefatal toanyraceoranybioticorabioticcomponents.components.

ItaimstofacilitatefarmersandconsumersandItaimstofacilitatefarmersandconsumersandhence itwasneverthoughttobepoisonratherhence itwasneverthoughttobepoisonratherthanpanacea.thanpanacea.

Thank ouforpatientlyhearingme.Thank ouforpatientlyhearingme.

Geneticallymodifiedfoodaremadesoasto lookGeneticallymodifiedfoodaremadesoasto lookafterthescarcityoffoodandresources, itsafterthescarcityoffoodandresources, its

objectivewasnevermeanttocreateanythingthobjectivewasnevermeanttocreateanythingthwouldbefatal toanyraceoranybioticorabioticwouldbefatal toanyraceoranybioticorabioticcomponents.components.

ItaimstofacilitatefarmersandconsumersandItaimstofacilitatefarmersandconsumersandhence itwasneverthoughttobepoisonratherhence itwasneverthoughttobepoisonratherthanpanacea.thanpanacea.

Thank ouforpatientlyhearingme.Thank ouforpatientlyhearingme.

MOUSAM RANJAN DASH ON TERMINATOR TECHNOLOGY

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