Mosquito Sampling and Techniques
description
Transcript of Mosquito Sampling and Techniques
![Page 1: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/1.jpg)
Mosquito Sampling Mosquito Sampling and Techniquesand Techniques
Dr. Russell Wright, Prof. Dr. Russell Wright, Prof. EmeritusEmeritus
![Page 2: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/2.jpg)
Classification Based on Classification Based on Larval Habitat Larval Habitat
Flood Water MosquitoesFlood Water Mosquitoes
Permanent Water MosquitoesPermanent Water Mosquitoes Permanent Pool GroupPermanent Pool Group Transient Water GroupTransient Water Group
Container MosquitoesContainer Mosquitoes
![Page 3: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/3.jpg)
Larval HabitatLarval Habitat Important to know which Important to know which
mosquitoes cause problems and mosquitoes cause problems and when.when.
Identify larval habitats based on Identify larval habitats based on adults present.adults present.
Most effective to find and map Most effective to find and map larval habitats and eliminate or larval habitats and eliminate or treat in this stage.treat in this stage.
![Page 4: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/4.jpg)
Sampling EquipmentSampling Equipment Vehicle to get around.Vehicle to get around. Good topographical map or at least a plat Good topographical map or at least a plat
or city map.or city map. Data sheets.Data sheets. Boots.Boots. Dipper, collecting jars, small straining Dipper, collecting jars, small straining
type net or large bulb syringe. type net or large bulb syringe. Water proof marking pens, tape etc. Water proof marking pens, tape etc. Carrying box or container, ice chest etc.Carrying box or container, ice chest etc.
![Page 5: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/5.jpg)
Sampling EquipmentSampling Equipment
DipperDipper PipetPipet AspiratorAspirator Flashlight Flashlight
AspiratorAspirator Larval ContainerLarval Container Bag for extra Bag for extra
vials, pens, vials, pens, labeling tape, labeling tape, etc.etc.
![Page 6: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/6.jpg)
How do you Survey How do you Survey for Larval Mosquito for Larval Mosquito
Habitats?Habitats?
![Page 7: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/7.jpg)
Floodwater SpeciesFloodwater Species Look for areas of standing water Look for areas of standing water
immediately after heavy rains or flooding.immediately after heavy rains or flooding. Most municipalities should have an idea Most municipalities should have an idea
of location of such problem areas.of location of such problem areas. Roadside ditches, low lying pools in Roadside ditches, low lying pools in
woodlands or field area.woodlands or field area. Must exist long enough for mosquito Must exist long enough for mosquito
larvae to develop. In spring 10-14 days. larvae to develop. In spring 10-14 days. In summer 6-10 days.In summer 6-10 days.
![Page 8: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/8.jpg)
Floodwater Mosquitoes – Floodwater Mosquitoes – Aedes Aedes vexansvexans
![Page 9: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/9.jpg)
Floodwater Mosquitoes – Floodwater Mosquitoes – Aedes Aedes vexansvexans
![Page 10: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/10.jpg)
Permanent Water Permanent Water MosquitoesMosquitoes
Permanent Pool Group Permanent Pool Group Usually fresh water, shallow grassy edges Usually fresh water, shallow grassy edges
of ponds, even lakes edges of slow moving of ponds, even lakes edges of slow moving rivers or streams. rivers or streams.
Generally these species are not serious Generally these species are not serious pests in Oklahoma and certainly probably pests in Oklahoma and certainly probably do not contribute much to the do not contribute much to the transmission of West Nile virus.transmission of West Nile virus.
Although these area may seem like the Although these area may seem like the logical areas to look often are least likely logical areas to look often are least likely to have pest mosquitoes species.to have pest mosquitoes species.
![Page 11: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/11.jpg)
Permanent Water Permanent Water MosquitoesMosquitoes
Transient Pool Group.Transient Pool Group. Most of the important species of Most of the important species of CulexCulex
that feed primarily on birds and likely are that feed primarily on birds and likely are the chief vector of WNV between birds the chief vector of WNV between birds and mosquitoes.and mosquitoes.
Any area of standing water that lasts for Any area of standing water that lasts for days to weeks can range from fairly fresh days to weeks can range from fairly fresh water around dripping faucets, plumbing, water around dripping faucets, plumbing, or irrigation systems to very polluted or irrigation systems to very polluted water in sewage lagoons.water in sewage lagoons.
![Page 12: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/12.jpg)
Sources of TransientSources of TransientPermanent WaterPermanent Water
Floodwater pools that are in low lying area Floodwater pools that are in low lying area that may persist for three or four weeks, that may persist for three or four weeks, especially if refilled by rain or overflow water. especially if refilled by rain or overflow water. Usually somewhat stagnant.Usually somewhat stagnant.
Low lying areas of storm sewers systems, Low lying areas of storm sewers systems, catch basins.catch basins.
Many cities and towns of Oklahoma do not Many cities and towns of Oklahoma do not have storm sewer system. Overflow rainwater have storm sewer system. Overflow rainwater runs down roadside ditches to catch basins, runs down roadside ditches to catch basins, creeks, etc. Often after water has quit flowing creeks, etc. Often after water has quit flowing and water stands in low areas. One key area and water stands in low areas. One key area often missed are drive way culverts of homes. often missed are drive way culverts of homes.
![Page 13: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/13.jpg)
Sources of Transient Sources of Transient Permanent Water cont.Permanent Water cont.
Water accumulations around leaking Water accumulations around leaking plumbing, irrigation systems, etc.plumbing, irrigation systems, etc.
Marshy low lying swampy areas. Marshy low lying swampy areas. Water that has accumulated in area such Water that has accumulated in area such
as abandoned cellars, sink holes, etc.as abandoned cellars, sink holes, etc. Water that has accumulated in tires or Water that has accumulated in tires or
other large containers.other large containers. Very stagnant foul water associated with Very stagnant foul water associated with
animal wastes, sewage treatment animal wastes, sewage treatment overflow.overflow.
![Page 14: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/14.jpg)
Transient Water Mosquito Transient Water Mosquito HabitatHabitat
![Page 15: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/15.jpg)
Container Breeding Container Breeding MosquitoesMosquitoes
Aedes albopictusAedes albopictus and some and some Culex.Culex.
Old tires or tire dumps, containers of Old tires or tire dumps, containers of any type in dumps, industrial areas, any type in dumps, industrial areas, backyards.backyards.
Birdbaths, flower pot containers, Birdbaths, flower pot containers, abandoned swimming pools or kiddy abandoned swimming pools or kiddy pools, pet water dishes, water gardens pools, pet water dishes, water gardens without good water flow. without good water flow.
![Page 16: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/16.jpg)
Aedes albopictusAedes albopictus Eggs in Eggs in ContainerContainer
Efficient container Efficient container breeder utilizing breeder utilizing available sources.available sources.
Eggs laid on Eggs laid on surface of water, surface of water, on sides of on sides of container, and on container, and on stick.stick.
Immediate egg Immediate egg hatch of some hatch of some eggs, delayed eggs, delayed hatch for others.hatch for others.
![Page 17: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/17.jpg)
Aedes albopictusAedes albopictus Eggs Eggs
![Page 18: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/18.jpg)
How Do You Survey How Do You Survey For Adult For Adult
Mosquitoes?Mosquitoes?
![Page 19: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/19.jpg)
CDC Miniature Light CDC Miniature Light TrapTrap
Developed by Developed by CDC for portable CDC for portable collection of collection of mosquitoes and mosquitoes and sand flies.sand flies.
Standard survey Standard survey tool for mosquito tool for mosquito collections.collections.
Operates on 6 Operates on 6 volts.volts.
![Page 20: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/20.jpg)
AttractantsAttractants COCO22 is the standard is the standard
attractant for attractant for mosquitoes. mosquitoes.
Increases trap Increases trap collections.collections.
200ml/min is 200ml/min is average release average release rate.rate.
COCO22 plus Octenol plus Octenol increases trap catch increases trap catch for some species.for some species.
![Page 21: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/21.jpg)
CDC Gravid TrapCDC Gravid Trap Designed to catch Designed to catch
gravid gravid CulexCulex females.females.
Females are Females are attracted to the attracted to the hay infusion as an hay infusion as an oviposition site.oviposition site.
May be used for May be used for virus detection.virus detection.
Operates on 6 Operates on 6 volts.volts.
![Page 22: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/22.jpg)
New Standard Miniature New Standard Miniature Light TrapLight Trap
Incandescent Trap.Incandescent Trap. New design New design
reflects light down reflects light down like a street light. like a street light.
Emits 75% more Emits 75% more light than other light than other incandescent traps incandescent traps with maximal air with maximal air flow.flow.
Operates on 6 Operates on 6 volts.volts.
![Page 23: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/23.jpg)
New Standard Miniature New Standard Miniature Light TrapLight Trap
Blacklight Trap.Blacklight Trap. Internal super Internal super
efficient transistor efficient transistor ballast drives 4 ballast drives 4 watt blue watt blue blacklight tube.blacklight tube.
Operates on 6 Operates on 6 volts.volts.
UV light may UV light may increase trap increase trap catches of some catches of some species.species.
![Page 24: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/24.jpg)
New Standard New Standard Miniature Light TrapMiniature Light Trap
BlacklightIncandescent Light
![Page 25: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/25.jpg)
New Standard Mini Light New Standard Mini Light Trap,Trap,
Options:Options: Photo-cell Photo-cell
released CO2 released CO2 option.option.
Allows release Allows release of CO2 when of CO2 when photo switch photo switch turns on light.turns on light.
![Page 26: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/26.jpg)
Omni-Directional Fay-Omni-Directional Fay-Prince TrapPrince Trap
Daytime trap, no Daytime trap, no light source.light source.
CollectCollect Aedes Aedes albopictus, albopictus, Aedes aegypti.Aedes aegypti.
Utilizes Utilizes contrasting contrasting black/white black/white colors with COcolors with CO22 to attract to attract mosquitoes.mosquitoes.
![Page 27: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/27.jpg)
Updraft Blacklight Updraft Blacklight TrapTrap
Proven effective Proven effective for collection of for collection of AnophelesAnopheles mosquitoes in mosquitoes in central central America.America.
Requires 12 Requires 12 Volts DC Volts DC operation.operation.
![Page 28: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/28.jpg)
New Jersey Light TrapNew Jersey Light Trap Typically Typically
mounted in a mounted in a permanent permanent position for position for mosquito mosquito collection.collection.
25 watt bulb.25 watt bulb. Requires 110 V Requires 110 V
AC operation.AC operation.
![Page 29: Mosquito Sampling and Techniques](https://reader033.fdocuments.net/reader033/viewer/2022061412/56815e0d550346895dcc68d8/html5/thumbnails/29.jpg)
For Further InformationFor Further Information Presentations may be accessed Presentations may be accessed
online at online at entoplp.okstate.eduentoplp.okstate.edu Click on Mosquitoes/WNVClick on Mosquitoes/WNV E-mail for Dr. Wright at E-mail for Dr. Wright at
[email protected]@okstate.edu