Morphological Dilation and Erosion for Particle Analysis ...€¦ · that "optimal" general...

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Morphological Dilation and Erosion for Particle Analysis: A Review Prabhdeep Singh and Vandana Chauhan Electronics & Communication Department, Kurukshetra University, Kurukshetra Email: [email protected], [email protected] AbstractIllumination is a standout amongst the most critical variables influencing the presence of a picture. It frequently prompts decreased structures and inhomogeneous forces of the picture because of various surface of the protest surface and shadows cast from various light source bearings. This impact creates from non uniform background enlightenment and its belongings are unfriendly in the event of biological images. Methods, for example, segmentation, edge detection and general picture handling calculations in view of 'region of interest' couldn't separate between a portion of the particles and their background or neighbouring pixels. This paper is expected to evacuate these issues in minute picture handling by right off the bat expelling the issue of non-uniform background brightening from the picture utilizing Morphological Opening, Adaptive Histogram Equalization and Image Enhancement to change the information picture to its listed frame with greatest exactness including thresholding and difference modification strategies lastly utilizing the associated segment methodology to discover number of particles present in the picture, making pseudo shaded list picture to figure the qualities of each molecule plainly and registering the attributes of each molecule lastly plotting zone based measurements and histogram of the last picture. Index Termsmorphology, histogram equalization, thresholding, dialation I. INTRODUCTION Image processing is utilized to adjust pictures to enhance them (improvement, reclamation), extricate data (investigation, acknowledgment), and change their structure (arrangement, picture altering). Pictures can be prepared by optical, photographic, and electronic means, however picture handling utilizing advanced PCs is the most widely recognized technique on the grounds that computerized strategies are quick, adaptable, and precise. Image handling innovation is utilized via planetary researchers to upgrade pictures of Mars, Venus, or different planets. Specialists utilize this innovation to control CT sweeps and MRI pictures. Picture Enhancement enhances the quality (clearness) of pictures for human survey. Evacuating obscuring and commotion, expanding difference, and uncovering points of interest are cases of improvement operations. Image Processing fundamentally incorporates examination, controls, Manuscript received March 1, 2017; revised July 11, 2017. stockpiling and show of graphical pictures from sources, for example, photos, drawings et cetera. Picture handling traverses a grouping of 3 stages, which are the picture obtain, preparing and show stage. The picture secures stage changes over the distinctions in shading and shading in the photo into twofold values that a PC can handle. The enhancement phase can include image enhancement and data compression. The last phase consists of display or printing of the processed image. The term morphology means form and structure of an object. Sometimes it refers to the arrangements and inter- relationships between the parts of an object. Morphology is identified with the shapes and advanced morphology is an approach to depict and examine the state of a computerized question. In science, morphology relates all the more specifically to state of a creature, for example, microorganisms. Morphological opening is a name particular innovation that makes a yield picture to such an extent that estimation of every pixel in the yield picture depends on a correlation of the comparing pixel in the information picture with its neighbors. By picking the size and state of the area, one can build a morphological operation that is delicate to particular shapes in the information picture. Morphological capacities could be utilized to perform regular picture preparing undertakings, for example, contrast enhancement, noise removal, thinning, skeletonization, filling and segmentation. II. RELATED WORK, ISSUES AND POSSIBLE SOLUTIONS There are many research papers that propose Histogram modelling systems to alter a picture so that its histogram has a coveted shape. This is helpful in extending the low-differentiate levels of a picture with a narrow histogram, accordingly accomplishing contrast improvement. This is driven by the absence of evacuation of non-uniform illumination, thus of which nearness of additional light pixels at a few positions in the picture and additional dull pixels around different particles in the picture is variable, so this difference improvement at the beginning of the picture handling does not make the exact limits of the items to be identified. [8] Komal Vij, et al. has proposed picture upgrade strategy to build picture perceivability and subtle elements. They covers every one of the components like enhancement efficiency, computational necessities, noise amplification, user ©2017 Int. J. Elec. & Elecn. Eng. & Telcomm. International Journal of Electrical and Electronic Engineering & Telecommunications Vol. 6, No. 3, July 2017 doi: 10.18178/ijeetc.6.3.11-14 11

Transcript of Morphological Dilation and Erosion for Particle Analysis ...€¦ · that "optimal" general...

Page 1: Morphological Dilation and Erosion for Particle Analysis ...€¦ · that "optimal" general differentiation is seen. IV. C. ONCLUSIONS AND . F. UTURE . W. ORK It has been inferred

Morphological Dilation and Erosion for Particle

Analysis: A Review

Prabhdeep Singh and Vandana Chauhan Electronics & Communication Department, Kurukshetra University, Kurukshetra

Email: [email protected], [email protected]

Abstract—Illumination is a standout amongst the most

critical variables influencing the presence of a picture. It

frequently prompts decreased structures and

inhomogeneous forces of the picture because of various

surface of the protest surface and shadows cast from

various light source bearings. This impact creates from non

uniform background enlightenment and its belongings are

unfriendly in the event of biological images. Methods, for

example, segmentation, edge detection and general picture

handling calculations in view of 'region of interest' couldn't

separate between a portion of the particles and their

background or neighbouring pixels. This paper is expected

to evacuate these issues in minute picture handling by right

off the bat expelling the issue of non-uniform background

brightening from the picture utilizing Morphological

Opening, Adaptive Histogram Equalization and Image

Enhancement to change the information picture to its listed

frame with greatest exactness including thresholding and

difference modification strategies lastly utilizing the

associated segment methodology to discover number of

particles present in the picture, making pseudo shaded list

picture to figure the qualities of each molecule plainly and

registering the attributes of each molecule lastly plotting

zone based measurements and histogram of the last picture.

Index Terms—morphology, histogram equalization,

thresholding, dialation

I. INTRODUCTION

Image processing is utilized to adjust pictures to

enhance them (improvement, reclamation), extricate data

(investigation, acknowledgment), and change their

structure (arrangement, picture altering). Pictures can be

prepared by optical, photographic, and electronic means,

however picture handling utilizing advanced PCs is the

most widely recognized technique on the grounds that

computerized strategies are quick, adaptable, and precise.

Image handling innovation is utilized via planetary

researchers to upgrade pictures of Mars, Venus, or

different planets. Specialists utilize this innovation to

control CT sweeps and MRI pictures. Picture

Enhancement enhances the quality (clearness) of pictures

for human survey. Evacuating obscuring and commotion,

expanding difference, and uncovering points of interest

are cases of improvement operations. Image Processing

fundamentally incorporates examination, controls,

Manuscript received March 1, 2017; revised July 11, 2017.

stockpiling and show of graphical pictures from sources,

for example, photos, drawings et cetera. Picture handling

traverses a grouping of 3 stages, which are the picture

obtain, preparing and show stage. The picture secures

stage changes over the distinctions in shading and

shading in the photo into twofold values that a PC can

handle. The enhancement phase can include image

enhancement and data compression. The last phase

consists of display or printing of the processed image.

The term morphology means form and structure of an

object. Sometimes it refers to the arrangements and inter-

relationships between the parts of an object. Morphology

is identified with the shapes and advanced morphology is

an approach to depict and examine the state of a

computerized question. In science, morphology relates all

the more specifically to state of a creature, for example,

microorganisms. Morphological opening is a name

particular innovation that makes a yield picture to such

an extent that estimation of every pixel in the yield

picture depends on a correlation of the comparing pixel

in the information picture with its neighbors. By picking

the size and state of the area, one can build a

morphological operation that is delicate to particular

shapes in the information picture. Morphological

capacities could be utilized to perform regular picture

preparing undertakings, for example, contrast

enhancement, noise removal, thinning, skeletonization,

filling and segmentation.

II. RELATED WORK, ISSUES AND POSSIBLE

SOLUTIONS

There are many research papers that propose

Histogram modelling systems to alter a picture so that its

histogram has a coveted shape. This is helpful in

extending the low-differentiate levels of a picture with a

narrow histogram, accordingly accomplishing contrast

improvement. This is driven by the absence of evacuation

of non-uniform illumination, thus of which nearness of

additional light pixels at a few positions in the picture

and additional dull pixels around different particles in the

picture is variable, so this difference improvement at the

beginning of the picture handling does not make the

exact limits of the items to be identified. [8] Komal Vij,

et al. has proposed picture upgrade strategy to build

picture perceivability and subtle elements. They covers

every one of the components like enhancement efficiency,

computational necessities, noise amplification, user

©2017 Int. J. Elec. & Elecn. Eng. & Telcomm.

International Journal of Electrical and Electronic Engineering & Telecommunications Vol. 6, No. 3, July 2017

doi: 10.18178/ijeetc.6.3.11-1411

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intervention, and application suitability. [10] Yan Wan,

et al. has proposed a double edge ascertaining technique

to acquire precise and nonstop fiber edge, and to control

the picture noise. [11] M. Kowalczyk, et al. has proposed

origination of viably working gatherings of morphology

capacities specifically picture cases. [12] David Menotti

has proposed two strategies for quick picture differentiate

improvement based on Histogram Equalization (HE), one

for gray-level images, and other for color images. For

gray-level images, technique called Multi-HE has been

proposed. [13] Ley, et al. has proposed a basic

background illumination adjustment based approach for

enhancing tangling issues with uneven or poor lit blue-

/green screens. [14] Joanna Sekulska, et al. has proposed

general techniques for organic pictures handling. These

procedures are arranged to better picture interpretation. [7]

has proposed a programmed strategy for assessing the

enlightenment field utilizing just picture power

gradients.[15] has proposed a novel model-based redress

technique is proposed, in view of the suspicion that a

picture ruined by force inhomogeneity contains more

data than the relating uncorrupted image. [9] has

proposed another way to deal with the revision of power

inhomogeneities in attractive reverberation imaging

(MRI) that fundamentally enhances force based tissue

segmentation. [3] Yadong Wu, et al. has proposed a

picture light revision calculation in view of tone mapping.

The proposed calculation consolidated shading space

decay and tone mapping based picture shine conformity,

which can enhance the picture differentiate while keeping

up the better shade of the first picture, and can't expand

noise. [4] M Rama Bai has Introduced a novel algorithm

based on multi-scale morphological method for the

purpose of border detection. Standard morphological

border detection methods use single and symmetrical

structure elements. [2] YanFeng Sun, et al. has proposed

a Multi-scale Fusion TV-based Illumination Normalized

(MFTVIN) display. In this Illumination impacts in the

expansive scale part are expelled by district based

histogram evening out and homomorphic filtering. [13]

Emerson Carlos Pedrino, et al. has proposed a unique

reconfigurable design utilizing sensible, number juggling,

and morphological directions created consequently by a

hereditary programming approach. They likewise

exhibited Binary, dark, and shading picture pragmatic

applications utilizing the created design.

III. TECHNOLOGY OVERVIEW

Mathematical Morphology (MM) is a hypothesis and

strategy for the examination and preparing of geometrical

structures, in light of set hypothesis, cross section

hypothesis, topology, and irregular capacities. MM is

most regularly connected to advanced pictures, yet it can

be utilized too on diagrams, surface cross sections, solids

and numerous other spatial structures, Topological and

Geometrical space ideas, for example, estimate, shape,

convexity,and geodesic separation, can be described by

MM on both persistent and discrete spaces. MM is

likewise establishment of morphological picture handling,

which comprises of an arrangement of administrators that

change pictures as indicated by the above

portrayals.Denoting an image by f(x) and the structuring

function by b(x), the grayscale dilation of f by b is given

by:

where "sup" denotes the supremum. Similarly, the

erosion of f by b is given by

MM was originally developed for binary images, and

was later extended to grayscale functions and images.

The subsequent generalization to complete lattices is

widely accepted today as MM's theoretical foundation. In

binary morphology, an image is viewed as a subset of

a Euclidean space Rd or the integer grid Zd, for some

dimension d.

Figure 1. (a) Original Image (b) Result of Dialation (c) Result of Erosion

A portion of the essential components of binary

morphology incorporates Structuring Element, Basic

Operators: Euclidean Distance and Shift Invariant,

Erosion and Dilation and Opening and Closing of double

Images. The essential thought in binary morphology is to

test a picture with a straightforward, pre-characterized

shape, reaching determinations on how this shape fits or

misses the shapes in the picture. This basic "test" is

called organizing component, and is itself a parallel

picture. Widening adds pixels to the limits of items in a

picture, while disintegration evacuates pixels on question

limits. In greyscale morphology, pictures are capacities

mapping an Euclidean space or network E into R U {∞, -

∞}, where R is set of reals, ∞ is a component bigger than

any genuine number, - ∞ is a component littler than any

genuine number. Grayscale organizing components are

additionally elements of a similar organization, called

"structuring functions".

a) b) c)

Figure 2. (a) The mug image; (b) Its histogram; (c) Thresholded using global segmentation at T=16. [16]

Various techniques and common approaches to solve

the problem of particle identification are Image Filtering,

©2017 Int. J. Elec. & Elecn. Eng. & Telcomm.

International Journal of Electrical and Electronic Engineering & Telecommunications Vol. 6, No. 3, July 2017

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Boundary detection, Edge Detection, Linear Filtering,

Segmentation, Morphological operations: Dilation and

Erosion etc. However, a large portion of these systems

alone neglect to precisely decide the items genuine limits

because of the issue of non-uniform brightening in the

background of the picture because of which the vast

majority of the particles give off an impression of being

either dim or light in a picture and utilizing methods, for

example, division, edge discovery and general picture

handling calculations in view of 'region of interest'

couldn't separate between a portion of the particles and

their background or neighbouring pixels.

Notwithstanding when the particles are extricated, there

are changes to their shape and size which prompts broken

readings in the calculations of range of such particles. In

this way, propelled picture handling and picture upgrade

devices must be utilized for most extreme precision of

the outcomes and to distinguish the particles precisely

from the picture without missing a solitary protest.

Figrue 3. Boundary extraction usng 3*3 square element. [4]

The Following is the flow-diagram of algorithms

followed in previous approaches to solve this desired

problem:

Figure 4. Image processing algorithm previously used.

Considering the above calculation, the minuscule

pictures are first experienced through histogram

equalization, a strategy that speaks to the relative

recurrence of event of dark levels inside a picture.

Histogram displaying systems change a picture so that its

histogram has a coveted shape. This is valuable in

extending the low-differentiate levels of a picture with a

tight histogram, subsequently accomplishing contrast

improvement. In histogram balance (HE), the objective is

to acquire a uniform histogram for the yield picture, so

that "optimal" general differentiation is seen.

IV. CONCLUSIONS AND FUTURE WORK

It has been inferred that because of non-uniform

background illumination, the vast majority of the

particles give off an impression of being either dull or

light in a picture and utilizing strategies, for example,

division, edge location and general picture preparing

calculations in light of 'region of interest' couldn't

separate between a portion of the particles and their

experience or neighbouring pixels. In future, it is plan to

play out the reenactments and investigation of a picture

and its upgrade to amend for non uniform enlightenment,

then utilize the improved picture to distinguish discrete

items/particles exhibit in the picture. The work to be

done is based upon pictures taken for natural reviews, for

example, pictures comprising of bunch of cells,

microorganisms, or different particles where it is critical

to discover the convergence of the particles. In this way,

a specific characterized zone of a photographic plate is

taken and uncovered by the particles the attributes of

which are to be registered. Thus, the strategy utilized is

make a calculation to at long last analyze each molecule

of the picture, to see obviously every question in the

picture, and evacuate any of the issues, for example, non-

uniform brightening, less brilliance and so forth that

make it hard to separate between the particles. At long

last, when all the perception and breaking down issues

are expelled, the attributes of every molecule, its region

must be processed and results would be appeared in

range based measurements and histogram equalization.

REFERENCES

[1] P. Lehana, et al., “Effect of enhancement technique uniform

ultrasound images,” Journal of Computational Engineering, 2015.

[2] Y. F. Sun, et al., “A multi-scale TVQ-based illumination normalization model,” Lecture Notes in Engineering and

Computer Science, vol. 1, pp. 1-6, 2011.

[3] Y. Wu, et al., “An image illumination correction algorithm based on tone mapping,” in Proc. 3rd International Congress on Image

and Signal Processing, 2010, pp. 245-248.

[4] M. R. Bai, “A new approach for border extraction using morphological methods,” International Journal of Engineering

Science and Technology, pp. 3832-3837, 2010.

[5] K. Loquin, et al., “Convolution filtering and mathematical

morphology on an image: A unified view,” pp. 1-4, 2010.

[6] P. Kupidur, “Semi-automatic method for a built-up area intensity

survey using morphological granulometry,” Problems of Landscape Ecology, pp. 271-277, 2010.

[7] A. Acharya, et al., “FPGA based non uniform illumination

correction in image processing applications,” Journal of Computer Technology and Applications, vol. 2, pp. 349-358, 2009.

[8] K. Vij, et al., “Enhancement of images using histogram processing techniques,” International Journal of Computer

Technology and Applications, vol. 2, pp. 309-313, 2009.

[9] J. M. Morel, et al., “Fast implementation of color constancy algorithms,” Proceedings of SPIE - The International Society for

Optical Engineering, vol. 19, pp. 2825-2837, 2009.

©2017 Int. J. Elec. & Elecn. Eng. & Telcomm.

International Journal of Electrical and Electronic Engineering & Telecommunications Vol. 6, No. 3, July 2017

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[10] Y. Wan, et al., “A dual threshold calculating method for fiber’s

edge extraction,” in Proc. International Conference on Intelligent

Computing and Intelligent Systems, 2009, pp. 247-254.

[11] T. Seemann, “Digital image processing using local segmentation,” thesis B. Sc (Hons), 2002, p. 61.

[12] D. Menotti, “Contrast enhancement in digital imaging using

histogram equalization,” Paris Est, pp. 1-85, 2008. [13] E. C. Pedrino, et al., “A genetic programming approach to

reconfigure a morphological image processing architecture,”

International Journal of Reconfigurable Computing, pp. 1-10, 2011.

[14] J. Sekulska, et al., “Digital image processing methods in

biological structure recognition a short review,” pp. 24-27, 2006.

[15] M. Ranzato, et al., “Automatic recognition of biological particles

in microscopic images,” pp. 1-19, 2006. [16] M. Kowalczyk, et al., “Application of mathematical morphology

operations for simplification and improvement of correlation of

images in close-range photogrammetry,” pp. 153-158, 2008. [17] N. Ley, et al., “GPU-Based background illumination correction

for blue screen matting,” in Proc. International Symposium on

Consumer Electronics, 2007, pp. 1-102.

©2017 Int. J. Elec. & Elecn. Eng. & Telcomm.

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