monsanto 08-23-05b

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  • 1. Corn Breeding OverviewAnkeny Investor Field Day August 22, 2005 Mark Messmer, Corn Commercial Breeding LeadMonsanto Company

2. MON Corn Breeding Keys To Success Structured to supportcommercial strategy Multiple channelsLine Review Worksheet Automating the Breeding 08_01DKD2*2/7640:@>0003.1001.Show selected lines only ExportAdd Filter? Lines Nursery notes YLD % of Check KeepPlot Lineage Mean SelectPedigree Trait 1 Trait 2 Trait 3 Comments x08_01DKD2*2/7640:@>0003.1001.110 14011 :@>0003.1001.4001.7 522 niceProcess08_01DKD2*2/7640:@>0102.1001. 95 X14012 :@>0003.1001.4002. 6 624 better08_01DKD2*2/7640:@>0126.1001. 85 Breeder Comments Advance this line into PCM1 on 6 testers x08_01DKD2*2/7640:@>0160.1002.12008_01DKD2*2/7640:@>0171.1003.101 x08_01DKD2*2/7640:@>0175.1002.10508_01DKD2*2/7640:@>0179.1003. 87 Data GCASCAChecksCheck mean Select Below and update above? Line Data Double click entry to show Set data sheet Driving experimental product Select SetEntryTester Gen # LocSELIN YLDMSTTWTYMRTLPSTLPPHT EHT CZINT FSP DRP ERM GCAAll 39120120 105 97120220105 100 75 75 100114 XC7201519HGZ1F413140250 18.5 56.3 1415115 53 199 113C8202 4 80IDM2F413103229 19.1 56.7 122 116 4434100114 XC8207 12 LH283F41311322619 57121 118 4254100115C8101 26:@>0003+F37 13224118 56136 112 51 299 113 numbers gameRank Data Ranks DataSetEntry Tester OverallLoc 1 Loc 2 Loc 3Loc 4Loc 5 Loc 6Loc 7Loc 8 Loc 9Loc 10 Loc 11 Loc 12 Loc 13 Loc 14 Loc 15 Sort by Yield Levels Mean Yield140 150160165 170175180 185190200210215300C7201 5 19HGZ1 14555663320 544 32 212 Green - Hybrid above chk mC8202 4 80IDM2185066332130 40 56 78 213 Red - Hybrid below chk meaC8207 12 LH2831660122030 8 854 10 12284 Germplasm DiversityC7201 5 19HGZ1 21 582547 36221 15 438C8202 4 80IDM2 42 5 224456 12115 822 1416 55C8207 12 LH2831614221110 1 1727 26 54 987 Broad and deep Marker Assisted LineDevelopment An SOP Enhancing the probability of breeding success Pipeline Depth More improved products2 3. Corn Breeding Structure is Designed to Support Multiple Commercial Channels10.9Linkages andCommercial Development0.80.7 Exchange of information0.6Line Development0.5 Are key to Success.0.4 Breeding Technology0.30.20.10LINENewBREEDING COMMERCIALCHANNELDEVELOPMENT Technology TECHNOLOGY BREEDING DEPLOYMENTBreeding Marker Labs F1 to national Product MON BrandsMANAGEMENT StatisticsCharacterization testing PRODUCT National Partners Disease and Insect Quality grain Channel Tech Regional PartnersSupport programs Winter Nurseries Local Partners Testing DiscoveryBreeding QA/QC Channel Strategy Channel Needs Comm Development 3 4. Regional Breeding Teams Tie Breeding Functions TogetherNorthW. CentralE. CentralSouth Regional Breeding Team Objectives: 1.Focus on most elite germplasm 2.Maintain important germplasm types 3.Enhance Product diversity 4.Enhance breeder germplasm knowledge and info exchange 5.Breeder synergy to identify and support customer needs 4 5. Expansive breeding and testing geography enables effective product identification and placement 5 6. State of the Art Automated Experimental Seed Processing Sets the Stage for Outstanding Field Data QualityShellingGrading Waste DiscardColor Sorting Finished Product Seed TreatmentCulls and Keeps Ready to Packet6 7. Investment in State of the Art Field Plot Equipment Enables Direct Mimicking of Farmer Practices and Timing~50% Increase in Plot Testing Capacity Over the Past Two Years7 8. Plant Breeding: What do we actually do? Plant Breeding is the science, art, and business of improving plants for human benefit. Easy Description Cross good germplasm by good germplasm Generate a series of genetically different exptl. parents Pick the right tester Select the best experimental line via extensive field testing More complicated Description delta(G)=1/C(0.25*k1*V(a)1.2/sqrt(V(P)1.2) + 0.25*k2*V(a)2.1/sqrt(V(P))2.1)8 9. Vast germplasm resources enable deployment of differentiated product portfolios 12 countries and 3 companies/country = 36 major germplasm acquisitionsAsgrowDEKALBIntlIntlAsgrow USA DEKALB USAInter-company Crosses Cargill Intl Holdens AgroceresSensako50% of breeding popn are inter-company crosses 9 10. The Dilemma:Germplasm Sources X Genes1 2 3 4 56 7 89 10 Corn has many thousands of genes, hundreds of which probably affect yieldOver 3 billion different genotypes result from just 2 forms of each of 20 genesYield from Parent 1 Yield from Parent 2 10 11. Marker-Assisted Recurrent Selection (MARS) rapidly increases the frequency of favorable genes Breeder1.0 F requency of F avorable G ene Selection and Tissue 0.9Recombination Sampling 0.8 0.7 0.6 0.5 0.4 Data C0C1C2 C3MultipleDNA extractionAnalysis cycles Multiple cycles of MARSincreases the frequencyof favorable markeralleles associated withGenerate Marker Fingerprintagronomic traitsGenotyping Lab 12. Marker Assisted Breeding: Sorting the Germplasm and GenesA Vastly Enhanced Numbers Game Mapping QTL position, size, and favorable parentYield from Parent 1Yield from Parent 2Fr eq u en cy o f :Cycle o f A Favo r ab leTh e BestSelect io n A llele Gen o t yp e 0 0 .5 1 2 p er t r illio nStart 1 0 .6 25 p er b illio n 2 0 .8 11 p er 5 0 0 0 3 0 .9 6 1 in 5End*Assumes 20 independent regions and 5% selection intensity 12 13. Breeders Build an Optimized Genetic Model for Every Breeding Population and use MARS to Toward the ModelSelect Traits Quality metrics Predicted Model Response Parental Contribution DataDataDecisions Identify and Select QTLsRUBICON: Integrate and Analyze Field and Marker data 13 14. Marker Characterization Early in the Breeding Enhances Probability of Success Late in the Process Genotypic and phenotypicXYear 1selection of parents Recombinegenes 6- to 8-year process 6- to 8-year processMarker Characterization 120,000+ Select best based ontedphenotype sis ctionAs er- t Sele rkMa urren Yield testcReYears 2-6Select bestYield test 10s Year 7Commercial product success rate