Monitor Signaling Pathway Activity in Any Cell - Lucerna-Chem · CignalTM Lenti Reporters Monitor...
Transcript of Monitor Signaling Pathway Activity in Any Cell - Lucerna-Chem · CignalTM Lenti Reporters Monitor...
CignalTM Lenti ReportersMonitor Signaling Pathway Activity in Any CellCignalTM Lenti Reporters
Cignal Lenti Reporters
The Cignal Lenti Reporters are ready-to-transduce lentiviral particles for assessing cell signaling activities in virtually any mammalian cell type. This system utilizes a unique combination of robust transcription factor reporter technology and superb lentiviral delivery power. Applications Study Signaling Pathway Activities in Impossible to Transfect Cell Types Immune Cells Stem Cells Neuronal Cells Differentiated Cells Primary Cells Other Difficult-to-Transfect Cells
Transient Transduction Studies to Study the Biological Impact of: Drug candidates or chemical compounds Gene knockdown (siRNA, shRNA) or gene overexpression events Peptides, proteins, or ligands
Generate Pathway Sensor Cell Lines Valuable cell-based assay platform for drug or gene function studies Why Cignal Lenti Reporters? Ready-to-transduce lentiviral particles
Reporter technology exhibiting exceptional sensitivity, specificity, and signal-to-noise ratio
Available with luciferase or GFP as reporter gene
How Cignal Lenti Reporters Work
tel: 301.682.9200 fax: 301.682.7300
Cignal LentiReporter
live lentivirusparticles
TransientTransduction Studies
Transduction w/ Cignal Lenti
AssayReadout
Treatment
Plate Cells
AntibioticSelection
Establish PathwaySensor Cell Lines
Clonal CellLine Isolation
Transductionw/ Cignal Lenti
Plate Cells
High Efficiency Lentiviral Delivery Made These Studies Possible.
PRIMARY CELL STUDIES: NFAT Lenti Reporter (luciferase) Measures PKC/Ca++ Pathway Signaling Activity in Primary Arteriole Smooth Muscle Cells9
Relative Luciferase Units
3
10
Cignal Lenti NFAT Reporter(luciferase) + DMSO
654
Cignal Lenti Reporter (luciferase)+ 10 ng/mL PMA + 0.5 mM Ionomycin
2
78
DIFFICULT TO TRANSFECT CELL LINES: RBP-Jk Lenti Reporter (luciferase) Measures Notch Signaling
Pathway Activity in Neuronal Cells (C6 Rat Glioma Cells)
90
Relative Luciferase Units
30
100
Cignal Lenti RBP-Jk Reporter [10 MOI]+ Cignal Lenti Renilla Control (luciferase) [1 MOI]
+ 100 MOI Ad-GFP
605040
Cignal Lenti RBP-Jk Reporter [10 MOI]+ Cignal Lenti Renilla Control (luciferase) [1 MOI]
+ 100 MOI Ad-NIC
20
7080
100
Cignal Lenti Reporters 16 Cignal Lenti Reporters are Available!
* Each tube contains 250 �L of lentiviral particles (~ 2.5 x 106 transducing units). The Cignal Lenti Reporters are safe replication imcompetent, HIV-based, VSV-G pseudotyped lentiviral particles ©2008 SABiosciences Corporation All Rights Reserved. Cignal™ & Cignal Finder™ are trademarks of SABiosciences Corporation.
Cignal Lenti Reporter System:Luc Cat #
C/EBP C/EBPcAMP/PKA CREBCell Cycle E2F/DP1p53/DNA Damage p53Hypoxia Hypoxia-inducible Factor-1 (HIF-1)Type I Interferon STAT1/STAT2Interferon Gamma STAT1/STAT1MAPK/ERK Elk-1/SRFMAPK/JNK AP-1c-myc Myc/MaxNF�B NF�BNotch RBP-JkPKC/Ca++ NFATRetinoic Acid Receptor Retinoic Acid Receptor (RAR)TGF� SMAD2/SMAD3/SMAD4Wnt TCF/LEF
CLS-001LCLS-002L CLS-003LCLS-004LCLS-007LCLS-008LCLS-009LCLS-010LCLS-011LCLS-012LCLS-013LCLS-014LCLS-015LCLS-016LCLS-017LCLS-018L
CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844CHF 844
CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874
Pathway Transcription Factor
6951 Executive Way, Frederick, MD 21703 USAwww.SABiosciences.com [email protected]
CLS-002G
CLS-010GCLS-011G
CLS-013GCLS-014G
CLS-018G
Cigna
l Len
ti Path
way R
eport
ers
Pack Size* PriceProduct Catalog #
Cigna
l Find
erTM
Lenti
Array
s Cignal Finder Lenti Cancer 10-Pathway Reporter ArrayCignal Finder Lenti Immune Response 10-Pathway Reporter ArrayCignal Finder Lenti Development 10-Pathway Reporter ArrayCignal Finder Lenti Toxicity 10-Pathway Reporter Array
CLA-001LCLA-002LCLA-003LCLA-004L
10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls10 Tubes + Pos/Neg Controls
CHF 2999CHF 2999CHF 2999CHF 2999
1-Tube Pack* 8-Tube PackProduct Catalog #
Cigna
l Len
ti Re
porte
r Con
trols
Cignal Lenti Positive Control (GFP)Cignal Lenti Negative Control (GFP)Cignal Lenti Positive Control (luciferase)Cignal Lenti Negative Control (luciferase)Cignal Lenti Renilla Control (luciferase)
CLS-PCGCLS-NCGCLS-PCLCLS-NCLCLS-RCL
CHF 844CHF 844CHF 844CHF 844CHF 844
CHF 1874CHF 1874CHF 1874CHF 1874CHF 1874
GFP Cat # 1-Tube Pack*8-Tube Pack
GFP REPORTER FORMAT: SRE Lenti Reporter (GFP) Measures MAPK/ERK
Pathway Activation in CV1 Fibroblasts
Fold Induction
1.5
0.50
Cignal Lenti SRE Reporter [50 MOI](Untreated)
32.52.0
Cignal Lenti SRE Reporter [50 MOI]+10% FBS + 10 ng/mL PMA
1
3.54
STABLE PATHWAY SENSOR CELL LINES: NF B Lenti Reporter (luciferase) Establishes aStable HEK293-NF B Pathway Sensor Cell Line
Relative Luciferase Units
60
20
0
120
100
80
40
140
160
180
P1w/o
TNF-�10 ng/mL
TNF-�
P5w/o
TNF-�10 ng/mL
TNF-�
P10w/o
TNF-�10 ng/mL
TNF-�
P15w/o
TNF-�10 ng/mL
TNF-�
Untreated Treated