Molecular response to toxic diatom-derived aldehydes in the sea … · 2017-02-02 · Molecular...
Transcript of Molecular response to toxic diatom-derived aldehydes in the sea … · 2017-02-02 · Molecular...
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Molecular response to toxic diatom-derived aldehydes in the sea urchin Paracentrotus lividus
Stefano Varrella
e-mail: [email protected] Stazione Zoologica Anton Dohrn, Naples, Italy
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• Relevant on structure of benthic marine community
• Mediterranean sea and Atlantic ocean
• Extraction and manteinance of gametes are easy
• Trasparent embryos
• Embryos grow rapidly (pluteus stage at 48hpf)
• Long reproductive period
physical and chemical xenobiotics
low pH
X-rays
UVs
CLASSIFICATION
Domain: Eukaryota
Kingdom: Animalia
Phylum: Echinodermata
Class: Echinoidea
Order: Echinoida
Family: Echinidae
Species: Paracentrotus lividus
antifoulings/pesticides
heavy metals
endocrine disrupters compounds
oxylipins derived from diatoms
• Good model for ecotoxicological studies on response
of marine invertebrate to environmental pollutants:
Model organism: Paracentrotus lividus
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There are more than 200 genera of living diatoms, and
approximately 100,000 species.
Diatoms live in the oceans and in freshwater.
Most live in open water, although some live as surface films at
the water-sediment interface (benthic), or even under damp
atmospheric conditions.
They are very important in oceans, where they are estimated to
contribute up to 45% of the total oceanic primary production.
Diatoms
http://deepbluehome.blogspot.it/search?q=diatom
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Beneficial role in supporting planctonic food web.
Diatoms are good food for the primary consumers of plankton.
Marine food web
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• Oocyte viability
• Sperm motility inhibition
• Fertilization success
• Cleavage inhibition
• Hatching reduction
• Larval toxicity
(Ianora and Miralto 2010)
Polychaetes
Echinoderms
Crustaceans
(Miralto et al. 1999)
Diatoms species produce secondary metabolities with cytotoxic activity
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Hydroperoxide lyase
(Ianora and Miralto 2010) OXYLIPINS
Oxidative metabolism of fatty acids in diatoms
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The effects of decadienal on sea urchin embryos
(Romano et al., 2010)
(Romano et al., 2011) (Marrone et al., 2012)
(Romano et al., 2003)
Decadienal
Control 1.32 µM 2.63 µM 3.95 µM 5.26 µM
Increasing concentrations of decadienal
Control 1.32 µM 2.63 µM 3.95 µM
Decadienal induces teratogenesis and apoptosis
First molecular studies
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….. The other PUAs?
Octadienal Heptadienal
Abundant compounds among PUAs
No molecular evidences
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Stress responses of sea urchin after exposure to
heptadienal and octadienal
Do PUAs affect the
embryogenesis?
When do PUAs act on
embryo development?
Morphologic Molecular
Do PUAs have molecular
targets?
Could these targets be
considered as possible
biomarkers for environmental
stress response?
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Sea urchin eggs
Incubation for 10 min with aldehydes
Fertilization
Morphological observations
at 48 hpf
Experimental procedures
Decadienal
Octadienal
Heptadienal
Control embryos
at 48 hpf
http://www.alnmag.com/news/2012/02/sperm-pathways-sea-urchins
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Do PUAs affect the embryogenesis?
Control (embryos in sea water without aldehydes)
PUAs treatments induced
TERATOGENESIS
(Varrella et al., 2014)
Dose-dependent increase of abnormal plutei
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Sea urchin eggs
Incubation for 10 min with aldehydes
Fertilization
Morphological observations at
72, 96 hpf until one-week
Experimental procedures
Decadienal
Octadienal
Heptadienal
Control embryos
at one week hpf
http://www.alnmag.com/news/2012/02/sperm-pathways-sea-urchins
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Do PUAs affect the embryogenesis?
Developmental times (hpf)
Ab
no
rma
l em
bry
os
(%) Decadienal Heptadienal Octadienal
Em
bry
os
nu
mb
er (
%)
72, 96 hpf
One week
Normal
Controllo
40’ df
Delayed
Control
Decadienal
Abnormal
Heptadienal
Octadienal
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Sea urchin eggs
Incubation for 10 min with aldehydes
Fertilization
Morphological observations
at 48 hpf
Experimental procedures
10 min pf 40 min pf
2 hpf
3 hpf 5 hpf 8 hpf PUAs additions 2 blastomeres 8 blastomeres 32 blastomeres Early blastula Late blastula
Control embryos
at 48 hpf
http://www.alnmag.com/news/2012/02/sperm-pathways-sea-urchins
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When do PUAs act on embryo development?
Concentrations (µM)
Ab
norm
al
Plu
tei
(%)
Heptadienal
Decadienal
Octadienal
10 min bf
10 min pf
40 min pf
2 hpf
3 hpf
5 hpf
8 hpf
(Varrella et al., 2014)
Aldehydes additions at different developmental times
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10 minutes before fertilization 10 minutes after
PUAs
PUAs action time
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Sea urchin eggs
Incubation for 10 min with aldehydes
Fertilization
Determination of teratogenic and dose-dependent
concentrations of aldehydes
Analysis of 31 by Real Time q-PCR
Experimental procedures
Decadienal
Octadienal
Heptadienal
http://www.alnmag.com/news/2012/02/sperm-pathways-sea-urchins
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Do PUAs have molecular targets?
Hsp70
Hsp60
Hsp56
MT
MTase
GS
cytb
p38 MAPK
14-3-3ε
hat
sox9
BP10
Blimp
Alix
Wnt5
Wnt6
Wnt8
SM30
BMP5-7
SM50
Nec
uni
p16
p19
MT
MT4
MT5
MT6
MT7
MT8
MDR1
CAT
Stress Skeletogenesis Development and
differentiation Detoxification
Genes analyzed by Real-Time qPCR
(Marrone et al., 2012; Varrella et al., 2014)
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The key stages of P. lividus embryogenesis
9 hpf
5 hpf
24 hpf
48 hpf
Early blastula Swimming blastula
Prism Pluteus
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Effects of decadienal on gene expressions at different
developmental stages
SM50
MT4
Hsp70
Hsp60
hat
MT6
SM30
uni
MT6
SM50
Wnt6
SM30
SM50 MT4
MT8
Stress
Skeletogenesis
Development/Differentiation
Detoxification
Up-regulated genes
Down-regulated genes (Modified from Varrella et al., 2014)
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Effects of heptadienal on gene expressions at different
developmental stages
Stress
Skeletogenesis
Development/Differentiation
Detoxification
Up-regulated genes
Down-regulated genes
(Modified from Varrella et al., 2014)
Wnt6
SM50
MT4
Hsp70
hat
p38 MAPK MTase
sox9
SM50
Hsp56
p38 MAPK
Alix hat Wnt5 Wnt6 Wnt8
p19 p16 uni SM50
SM30
SM50 MT4
MT8
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Effects of octadienal on gene expressions at different
developmental stages
Stress
Skeletogenesis
Development/Differentiation
Detoxification
Up-regulated genes
Down-regulated genes
(Modified from Varrella et al., 2014)
Wnt6
SM50 SM30 BMP5-7 p19 p16
MT4
Hsp70
MTase
hat
SM30
SM50 SM30
SM50
MTase SM50
SM30
SM50
MT6
MT7
CAT
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Stress
Skeletogenesis
Development/Differentiation
Detoxification
Up-regulated genes
Down-regulated genes
(Modified from Varrella et al., 2014)
Decadienal Heptadienal Octadienal
Hsp70 Hsp60
SM50 SM30 uni
hat
Wnt6
MT4 MT6 MT8
Hsp70 MTase hsp56 p38 MAPK
SM50 uni p19
Wnt5 Wnt6 Wnt8 hat sox9 Alix
MT4 MT6 MT8
Hsp70 MTase
SM50 SM30 BMP5-7 p16 p19
hat Wnt6
CAT MT4 MT6 MT8
Summary
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Do PUAs affect the sea urchin embryogenesis?
When do PUAs act on embryo development?
The aldehydes induced teratogenesis on sea urchin embryos in a dose-dependent
manner.
The aldehydes could compromise the normal embryonic development affecting
embryos before and/or soon after the fertilization
The teratogenic effect after a week of PUAs exposure appeared to be stronger for
decadienal and heptadienal.
Morphological conclusions
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Do PUAs have molecular targets? And could these targets be considered as possible
biomarkers for environmental stress response?
The aldehydes have different molecular targets, affecting the expression
levels of different genes at different times of embryonic development.
Stress response Biomarkers
Molecular conclusions
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....more in general
Effects of many other Oxylipins (HEPEs)
Functional networks of the PUAs target genes
Oxyditave stress effects induced by Oxylipins
Ecological relevance considering the importance of diatoms blooms in nutrient-rich
acquatic environments…..
.....molecular evidence for the toxic effects of the diatom-derived PUAs
.....novel tools for understing the cellular mechanisms of the response to aldehydes
exposure to the benthic organisms.
Future perspectives