Molecular biology folder.

IMPORTANT GENE-REGULATION PROTEINS

PINPOINTED BY NEW METHOD

ANDPOLAR GROWTH AT THE

BACTERIAL SCALE REVEALS POTENTIAL NEW TARGETS FOR ANTIBIOTIC

THERAPY.

Laura Maria Másmela gilUniversity medicine student lll semesterJanuary 23, 2012

FOLDING

INTRODUCTION

A novel technique has been developed and demonstrated at Penn State to map the proteins that read and regulate chromosomes

An international team of microbiologists led by Indiana University researchers has identified a new bacterial growth process , one that occurs at a single end or pole of the cell instead of uniform

ScienceDaily (Jan. 18, 2012)

PART A

IMPORTANT GENE-

REGULATION PROTEINS

PINPOINTED BY NEW METHOD


PART B

IMPORTANT GENE-REGULATION PROTEINS PINPOINTED BY NEW METHOD

•The new technique precisely pinpoints their location, and has the potential to take high resolution snapshots of proteins as they regulate or miss regulate an entire genome.

PART C


The research process began by their using a molecular tool called an exonuclease to remove DNA that is not bound by one of the gene-regulating proteins. They then determined the nucleotide sequence for each of the remaining protein-bound DNA bundles.

This technique is known as CHIP-EXO


PART D

Technique CHIP-EXO

Reduce any binding site single nucleotide THROUGH million nucleotides of the

genomes.

provides an unprecedented view of how genes are regulated.

is a low-noise technique that reveals two to five times more binding sites,

which provides a much more complete picture of the genes that are regulated by

a particular protein, and a greater understanding of

their structural organization across genomes .




PERSONAL COMMENT

Its important know about the gene-regulation proteins, because this allows to determine the different type of cells, and a better picture of how the gene pathways work in the normal development of the human body and when a pathology exist.


POLAR GROWTH AT

THE BACTERIAL

SCALE REVEALS

POTENTIAL NEW

TARGETS FOR

ANTIBIOTIC THERAPY.ScienceDaily (Jan. 17,

2012)

PART A

PART B


Based on past detailed studies of rod-shaped bacteria such as Escherichia coli and Bacillus subtilis, it has been assumed that most bacteria grow by binary fission, a dispersed mode of growth involving insertion of new cell wall material uniformly along the long axis of the cell. Growth requires breaking the cell wall at numerous places along the cylinder to allow insertion of new cell wall material, enabling uniform elongation of the cell, with the process culminated by cleavage at the mid-point of the cell to create two symmetric new cells.

PART C

Outer membrane proteins of an Agrobacterium tumefaciens cell were labeled in red, with images taken every 50 minutes as the cell grew. In panels three and four it is clear that the cell on the left (red) has kept all the labeled proteins, whereas the other cell has all new surface proteins.

Credit: Image courtesy of Indiana University


PART D

using time-lapse microscopy and transmission electron microscopy was observed using four species of bacteria

Polar growth of four bacterial species , the plant symbiont Sinorhizobium meliloti, the plant pathogen Agrobacterium tumefaciens and the human pathogens Brucella abortus

and Ochrobactrum anthropi

these bacteria are members of a class of bacteria called the Alphaproteobacteria.

the results show that polar growth is present in different types of bacteria including types that are outside the classroomalphaproteobacteria.


POLAR GROWTH AT THE BACTERIAL SCALE REVEALS POTENTIAL NEW TARGETS FOR

ANTIBIOTIC THERAPY.

PERSONAL COMMENT

This process of bacterial growth is important because from the molecular biology scientist can do some procedures, the objective is improve the clinical practice , from study cellular method and antibacterial strategies to therapies with organs, systems, and whole human body, preventing diseases.


MEDICAL

UTILITY

the study of molecular

structures facilitate better

understanding of medicine in their practice.

MEDICAL UTILITY

MEDICAL UTILITY

investigations allow this type of progress in medical

treatment, as both take preventive measures

on the development of some diseases caused

by specific characteristics of the organisms that had

never been explored in detail. these studies promo

te better health in the human population.

MEDICAL UTILITY

gene regulatory proteins

allows a more complete vision of the normal and abnormal

human development in the disease

polar growth of bacteria

antibiotic therapy allowed.

PROMOTION AND DISEASE PREVENTION

Important Gene-Regulation Proteins Pinpointed by New Method.sciencedaily.2012,january 18, from http://www.sciencedaily.com/releases/2012/01/120118132330.htm

Polar Growth at the Bacterial Scale Reveals Potential New Targets for Antibiotic Therapy.sciencedaily.2012,january 17, from http://www.sciencedaily.com/releases/2012/01/120117191421.htm

MARTINEZ S. LINA MARIA, Biología molecular. 6 ed. Medellin; UPB.fac. de medicina 2011, p 10-14;42-55.

BIBLIOGRAFY

http://www.sciencedaily.com/releases/2012/01/120118132330.htm




THANKS

Molecular biology folder.

Technology

Transcript of Molecular biology folder.