JERAD LIBHART5803 Woodcraft

San Antonio, TX 78218Home: (210)-368-9505 Cell: (970)-587-5683

jeradl@hotmail.comwww.linkedin.com/in/jeradl/

QUALIFICATIONS AND HIGHLIGHTS: Over 11 years experience in biotech, pharma, and oil & gas industries spanning product development,

manufacturing, and research granting me a unique view of processes and implementation BSL2 lab techniques, safety training, and Air Force security training Protein and antibody purification experience with transfer and validation of processes to manufacturing Bacterial fermentation/bioreactors and purification of proteins experience Design and execution of experiments; data collection, analysis, interpretation, reference, and reporting Strong technical documentation experience; I’ve written Test Procedures, Test Summary Reports,

SOPs, Progress Reports, and Batch Records as well as researching scientific journals for new methods Flow cytometers, Gen•S, MAX•M, STKS, AcT, MDII, and S Plus IV operation and maintenance Familiar with Western Blotting, ELISA, SDS-PAG electrophoresis, bioassays, water testing,

DNA/RNA methods, Real Time qPCR, Real Time qRT-PCR, and Karl Fischer titration Gas chromatography, Ion chromatography, Gas chromatography/Mass spectrometry, HPLC, FPLC,

liquid chromatography, and column chromatography Performing automated nucleic acid extractions with Biomerieux NucliSENS EasyMAG and manual

extractions with Qiagen RNeasy and DNeasy kits PCR and RT-PCR on Applied Biosystems 7500 Fast Real-Time System, Applied Biosystems

GeneAmp PCR System 9700, Bio-Rad CFX96, and Bio-Rad Molecular Imager Gel Doc XR+ System TaqMan probes and Invitrogen SuperScript III Platinum One-Step Quantitative Kit use UV/Vis/NIR Spectroscopy

RELEVANT EMPLOYMENT HISTORY:

Engineering Technician III 2014 – 2015KCI (through E-Soultions), San Antonio, TX (temp position)

Competitive testing of KCI medical devices vs competitor products R&D test development and design Writing test reports and protocols Data analysis Usage of DAQs, flow meters, and other common lab measurement equipment

Lab Manager 2013 - 2014Calumet Specialty Products San Antonio, TX

5 direct reports, employee scheduling, and personnel issues Knowledge of ASTM methods and Federal/State regulations Writing of SOPs, QC policies, and Technical communications Management of budget, lab supplies, in house testing, and outsource testing Coordinated installation and managed a new LIMS system now running company wide Responsible for design and implementation of a new QC system with all appropriate

documentation policies GC, D86, wet bench tests, Flash Point, Copper/Silver Corrosion, ChemStation, ChromPerfect,

DHA, Freeze, Cloud, Pour Point, and various ASTM tests for fuels and solvents Implemented new test methods and equipment per ASTM to bring gasoline production online DOT Hazardous Materials certified and Transportation of Dangerous Goods IATA certified

QC Scientist II 2011 - 2013DPT Laboratories, San Antonio, TX (left to work at Calumet)

New chemical component formulation, qualification, and validations Knowledge of FDA, USP, EP(PhEur), BP, and JP requirements/monographs, ASTM/AOCS Writing of change controls, deviations, SOPs, SMAs, test standards, summary reports,

memos, protocols Analysis and design of methods, recommendations where needed to customers Participation in meetings with pharmaceutical clients HPLC, GC, Empower, titrations, wet bench tests, TLC, IR, LOD, ROI, etc. per USP Data review of all tests and chemicals for release ensuring compliance for entire lab

Senior Research/Chemical Analyst 2011 - 2011Southwest Research Institute (SWRI) (through Aerotek), San Antonio, TX (temp position)

Gas chromatography on Varian CP-3800 GC and Varian CP-4900 micro-GC Ion chromatography on Dionex ICS-2000 (HPLC) Chromeleon and Galaxie software Gas chromatography/Mass spectrometer on Agilent Technologies 5975B inert XL MSD

GC/MS with ChemStation software Titrations and wet bench work Monitored a 24/7 bromination reaction for catalytic cracking of fuels

Molecular Medical Laboratory Technician 2009 - 2010DOD Global Pandemic Influenza Surveillance Program (lay off after slow down)US Air Force School of Aerospace Medicine/Public Health and EpidemiologyHenry M Jackson Foundation for the Advancement of Military Medicine Brooks City Base, TX

Automated total DNA/RNA extraction and purification with Biomerieux NucliSENS EasyMAG and manual extractions with Qiagen RNeasy and DNeasy kits

Real-time RT-PCR of viral isolates following CDC procedure for influenza samples TaqMan probes and Invitrogen SuperScript III Platinum One-Step Quantitative Kit use Mammalian cell cultures, storage, archival, and scraping Detection of swine flu (H1N1), influenza A, B, H1, H3, Norovirus by qRT-PCR Position of Trust security clearance; CLIA certified lab Adenovirus 14 RT-PCR, SDS-PAGE, and fluorescent detection with Bio-Rad Molecular

Imager Gel Doc XR+ System Growing, isolating, and killing viruses; routine cell cultures PCR and RT-PCR on Applied Biosystems 7500 Fast Real-Time System, Applied

Biosystems GeneAmp PCR System 9700, Bio-Rad CFX96, and Bio-Rad Molecular Imager Gel Doc XR+ System, High Resolution Melt Software v2.0

BSL2 lab techniques, safety training, and Air Force security training Data collection, analysis, interpretation, reference, and reporting

Chemist – Lab Tech Associate 2008 - 2009Halliburton, Vernal, UT (lay off)

ASTM testing of oil well cements and additives; viscosity and hardness testing Fluid loss, free water, and settling analysis by ASTM methods Set up remote access of computerized instruments Lean Six Sigma, participant in a Kaizen event, 5S Water chemical tests for alkalinity, total hardness, Ca, Chlorides, K, Na, Sulfates, pH,

resistivity, conductivity, and specific gravity of fluids by ASTM methods Determination of unknown substances Raw material, in process, and finished product quality testing Fann HPHT (high pressure high temperature) Consistometers, Rheometers, and

Viscometers, UCAs, atmospheric consistometer, crush strengths Redesigning water testing methods to get rid of hazardous materials Certified for OSHA, H2S Awareness, and CPR/First Aid Systems analysis and troubleshooting for both instrument hardware and software Data collection, analysis, interpretation, reference, and reporting

Quality Control Specialist (Frac Lab) 2007 - 2008Calfrac Well Services, Platteville, CO (lay off)

Quality control testing of current fracturing fluids (biopolymers); rheology and viscosity Development of new fracturing fluids system for Mexico

QC testing of bulk chemicals and determination of unknown substances Karl Fischer moisture testing on an Aquastar AQC34, including method development and

reagent changes for diesel fuel Water chemical tests for alkalinity, total hardness, Ca, Chlorides, K, Na, Sulfates, and specific gravity of fluids by ASTM methods UV/Vis/NIR spectrophotometers, pH meters, resistivity, and conductivity Carbohydrate titrations; Acid/base titrations Brookfield PVS rheometers, Fann viscometer, sand sieves by ASTM methods Systems analysis and troubleshooting for both instrument hardware and software Design and execution of experiments; data collection, analysis, interpretation, reference,

and reporting Raw material, in process, and finished product quality testing Rheid vapor pressure testing

Grants Research Associate (Biology Dept.) 2004 - 2005Colorado State University – Pueblo, Pueblo, CO (contract completed)

Lab Manager for a NIH funded project studying the effect of xanthotoxin on the reproductivity of rats and mice.

Western Blotting Real Time qPCR, Real Time RT-qPCR with Bio-Rad CFX96

DNA/RNA manual extractions with Qiagen kits, quality, and quantification methods Mammalian cell cultures Animal care (mice, rats, hamsters), handling, surgery, organ removal, and injections Bioassay plates using Bio-Rad plate readers X-ray equipment usage and maintenance Method and experimental design and development; GLPs Data collection, analysis, interpretation, reference and reporting Wrote Test Procedures, Test Summary Reports, SOPs, and Progress Reports Evaluation of new instrument capabilities Scientific literature research and review Systems analysis and troubleshooting for both instrument hardware and software

Worked various positions outside my main fields while looking for work 1999-2004

Special Projects Coordinator (Education and Training Program) 1999-1999North Carolina Biotechnology Center, Research Triangle Park, NC (temp position)(through Tech Specialists)

Participated in course design and content for a Process Operator course to train peoplefor the pharmaceutical, biotechnology, and chemical industries; GLPs, GMPs

Responsible for submitting industry safety guidelines, procedures, and methodology Responsible for review and material submission of Keller’s OSHA Safety Handbook Generated and utilized contacts within local biotech/pharmaceutical companies and

universities to gain their organizations’ cooperation as resources in NCBC projects Coordinated large group meetings involving representatives from multiple local

biotech/pharmaceutical companies Scientific literature research and review Preparation of group briefings and training materials

Research Associate (Cellular Analysis Division - Product Development) 1996-1999Beckman-Coulter, Miami, FL (lay off after buy out)

Wrote Test Procedures, Test Summary Reports, and SOPs Wrote Batch Records for transfer and validation of processes to manufacturing

Participated in the design and optimization of processes for the manufacture, filling, and lyophilization of two reagents consisting of CD4/CD8 antibodies bound to a latex particle. This included column chromatography purification of the antibody and crosslinker attachment.

Co-supervisory over manufacturing employees during manufacture, filling, lyophilization, and capping of several reagent lots for transfer and validation

Designed testing of reagent compatibility with filling equipment Worked with HIV infected Human blood RBC fixation methods for goat, shark, alligator, and goose RBCs to force fixed size and

biochemical properties for cell inclusion in AcT Tron Cell Control Karl Fischer moisture analysis method development and calculations for determination

on the Mettler Toledo DL-50 Titrator for a lyophilized reagent containing ketone groups Histogram overlay analysis for study of a lytic reagent Proposed new reagent packaging idea that more than doubled shelf life. Operation, maintenance, and calibration of particle counters and hematology diagnostic

analyzers AcT, MDII, S Plus IV, and ZBI Particle Counter Flow cytometers, Gen•S, MAX•M, and STKS used daily Performed high through-put reagent assays; MS Excel 97 spreadsheet data analysis Continually performed the following reagent testing, data collection, and analysis:

- Stability - Homogeneity - Time Matrix

- Linearity - Reproducibility - Within Run Precision- Interlaboratory Precision - Vacuum Fill and Accuracy

HPLC data analysis experience Yearly ISO 9001, GMP/GLP, HAZMAT, Bloodborne Pathogens refresher training BSL2 lab techniques and safety training

Design and execution of experiments; data collection, analysis, interpretation, reference, and reporting

Evaluation of new instrument capabilities Scientific literature research and review Systems analysis and troubleshooting for both instrument hardware and software

Identified three measured parameters for a raw material that point to why some lots had failed QC monodispersity testing. These priorly unused parameters explained the failure. Defined limits on these parameters will prevent the material manufacturer from sending unusable lots saving time and money.

Protein Purification Scientist I (Hemostasis Dept.) 1996-1996Dade International (merged into Dade Boehringer then into Siemens), Miami, FL (temp position)(through Lab Support Temporary Services)

Responsible for the purification of human tissue factor protein from a genetically altered strain of E. coli., including Batch Records and Deviation Reports

Active in the process of applying, revising, and validating a new R&D procedure utilizing affinity column chromatography.

Fermentation of genetically altered bacteria in bioreactors Knowledge of FDA regulations, GMPs, and GLPs as they apply to the position.

Although a temporary and only in my position a few months my manager gave me solo responsibility for Fragment F2 Concentrate procedure. Human Prothrombin was digested by Bovine Factor XA then the fragment F2 was purified from the digest by liquid column chromatography. I caught several errors that had been introduced into the documented procedure due to a Change Request two years earlier. These errors had resulted in lowered purity and yield of the F2 fragment. Upon implementation of the corrections with managerial approval, I achieved the highest purity and yield results they had seen in three years for the F2 fragment.

Equipment and methods used in my work are as follows: Pharmacia FPLC (fast protein liquid chromatography), Pharmacia BioPilot (liquid chromatography), Pharmacia LKB Phastsystem (electrophoresis), conductivity meter, pH meter, centrifuges, MLA Electra 800 Automatic Coagulation timer, APV Gaulin Homogenizer, Beckman DU 7500 Spectrophotometer, and a 30L Micros Fermentor/Bioreactor. Types of columns and chromatography used are as follows:

Phenyl Sepharose column – hydrophobic interaction chromatographySP Sepharose column – cation exchange chromatographyMono S column – cation exchange chromatographyQ Sepharose column – cation exchange chromatographyAntibody bound column – affinity chromatography

Utilized ELISAs, SDS-PAG electrophoresis, and UV/Vis/NIR spectroscopy BSL2 lab techniques and safety training

Data collection, analysis, interpretation, reference, and reporting Wrote Test Procedures, Test Summary Reports, SOPs, and Batch Records

Raw material, in-process, finished product and stability testing experience Evaluation of new instrument capabilities Validation, scale up, optimization, process development, and production work Systems analysis and troubleshooting for both instrument hardware and software

QC Polymer Chemist 1995-1995Chem Polymer (through Snelling Temporary Services), Fort Myers, FL (temp position)

Performed QC testing of the physical and chemical properties of nylon based plastics

Independent Study 1994-1995Youngstown State University Youngstown, OH

Completed a research project at YSU analyzing DNA by agarose gel electrophoresis, ethidium bromide staining, use of plasmid vectors, maintaining transformed JM101 E.

coli cultures, identifying transformed JM101 E. coli by ampicillin resistance, and determining sample DNA length after isolation.

EDUCATION: B.S. in Biology (specializing in Molecular Biology) with a minor in ChemistryYoungstown State UniversityRelevant Coursework: Organic Chemistry Microbiology Genetics Molecular Biology of the Cell Gene Manipulation Molecular Biology of the Gene Histology Immunology OSHA Certified GISH, Uintah Basin Applied Technology College, 2008Hydrogen Sulfide (H2S) Awareness, UBATC, 2008CPR/First Aid Certified, UBATC, 2008DOT Hazardous Materials Shipping certified, ERCweb, 2013Transportation of Dangerous Goods IATA certified, ERCweb, 2013

ADDITIONAL SKILLS:COMPUTER SKILLS: Win 98/XP/Vista/Windows 7, MS Office (Word, Excel, Power Point), and computer hardware/software (I build and fix computers)