Molbiol 2011-04-metabolism
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Transcript of Molbiol 2011-04-metabolism
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Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings
ЛЕКЦИЯ 3-2
Метаболизм
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Metabolism: The sum of the chemical reactions
in an organism
Catabolism: The energy-releasing processes
Anabolism: The energy-using processes
Microbial Metabolism
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Microbial Metabolism
Catabolism provides the building blocks and energy for
anabolism.
Figure 5.1
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A metabolic pathway is a sequence of enzymatically
catalyzed chemical reactions in a cell.
Metabolic pathways are determined by enzymes.
Enzymes are encoded by genes.
PLAY Animation: Metabolic Pathways (Overview)
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The collision theory states that chemical reactions can
occur when atoms, ions, and molecules collide.
Activation energy is needed to disrupt electronic
configurations.
Reaction rate is the frequency of collisions with enough
energy to bring about a reaction.
Reaction rate can be increased by enzymes or by
increasing temperature or pressure.
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Enzymes
Figure 5.2
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Enzymes
Biological catalysts
Specific for a chemical reaction; not used up in that
reaction
Apoenzyme: Protein
Cofactor: Nonprotein component
Coenzyme: Organic cofactor
Holoenzyme: Apoenzyme plus cofactor
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Enzymes
Figure 5.3
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Important Coenzymes
NAD+
NADP+
FAD
Coenzyme A
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Enzymes
The turnover number is generally 1-10,000 molecules
per second.
PLAY Animation: Enzyme–Substrate Interactions
Figure 5.4
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Enzyme Classification
Oxidoreductase: Oxidation-reduction reactions
Transferase: Transfer functional groups
Hydrolase: Hydrolysis
Lyase: Removal of atoms without hydrolysis
Isomerase: Rearrangement of atoms
Ligase: Joining of molecules, uses ATP
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Factors Influencing Enzyme Activity
Enzymes can be denatured by temperature and pH
Figure 5.6
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Factors Influencing Enzyme Activity
Temperature
Figure 5.5a
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Factors Influencing Enzyme Activity
pH
Figure 5.5b
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Factors Influencing Enzyme Activity
Substrate concentration
Figure 5.5c
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Factors Influencing Enzyme Activity
Competitive inhibition
Figure 5.7a–b
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Factors Influencing Enzyme Activity
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Factors Influencing Enzyme Activity
Noncompetitive inhibition
Figure 5.7a, c
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Factors Influencing Enzyme Activity
Feedback inhibition
Figure 5.8
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Ribozymes
RNA that cuts and splices RNA
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Oxidation-Reduction
Oxidation is the removal of electrons.
Reduction is the gain of electrons.
Redox reaction is an oxidation reaction paired with a
reduction reaction.
Figure 5.9
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Oxidation-Reduction
In biological systems, the electrons are often
associated with hydrogen atoms. Biological oxidations
are often dehydrogenations.
Figure 5.10
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The Generation of ATP
ATP is generated by the phosphorylation of ADP.
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The Generation of ATP
Substrate-level phosphorylation is the transfer of a
high-energy PO4– to ADP.
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The Generation of ATP
Energy released from the transfer of electrons
(oxidation) of one compound to another (reduction) is
used to generate ATP by chemiosmosis.
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The Generation of ATP
Light causes chlorophyll to give up electrons. Energy
released from the transfer of electrons (oxidation) of
chlorophyll through a system of carrier molecules is
used to generate ATP.
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Metabolic Pathways
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Carbohydrate Catabolism
The breakdown of carbohydrates to release energy
Glycolysis
Krebs cycle
Electron transport chain
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Glycolysis
The oxidation of glucose to pyruvic acid produces ATP
and NADH.
Figure 5.11, step 1
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1
3
4
5
Preparatory Stage
Two ATPs are
used
Glucose is split to
form two Glucose-
3-phosphate
Figure 5.12, step 1
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9
Energy-Conserving Stage
Two Glucose-3-
phosphate oxidized to
two Pyruvic acid
Four ATP produced
Two NADH produced
Figure 5.12, step 2
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Glycolysis
Glucose + 2 ATP + 2 ADP + 2 PO4– + 2 NAD+
2 pyruvic acid + 4 ATP + 2 NADH + 2H+
PLAY Animation: Glycolysis
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Alternatives to Glycolysis
Pentose phosphate pathway
Uses pentoses and NADPH
Operates with glycolysis
Entner-Doudoroff pathway
Produces NADPH and ATP
Does not involve glycolysis
Pseudomonas, Rhizobium, Agrobacterium
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Cellular Respiration
Oxidation of molecules liberates electrons for an
electron transport chain.
ATP is generated by oxidative phosphorylation.
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Intermediate Step
Pyruvic acid (from
glycolysis) is oxidized
and decarboyxlated.
Figure 5.13 (1 of 2)
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Krebs Cycle
Oxidation of acetyl CoA produces NADH and FADH2.
PLAY Animation: Krebs Cycle
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Krebs Cycle
Figure 5.13 (2 of 2)
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The Electron Transport Chain
A series of carrier molecules that are, in turn, oxidized
and reduced as electrons are passed down the chain.
Energy released can be used to produce ATP by
chemiosmosis.
PLAY Animation: Electron Transport Chains and Chemiosmosis
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Copyright © 2006 Pearson Education, Inc., publishing as Benjamin Cummings Figure 5.11
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Chemiosmosis
Figure 5.16
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Chemiosmosis
Figure 5.15
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Respiration
Aerobic respiration: The final electron acceptor in the
electron transport chain is molecular oxygen (O2).
Anaerobic respiration: The final electron acceptor in the
electron transport chain is not O2. Yields less energy
than aerobic respiration because only part of the Krebs
cycles operations under anaerobic conditions.
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Electron acceptor Products
NO3– NO2
–, N2 + H2O
SO4– H2S + H2O
CO32 – CH4 + H2O
Anaerobic Respiration
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Pathway Eukaryote Prokaryote
Glycolysis Cytoplasm Cytoplasm
Intermediate step Cytoplasm Cytoplasm
Krebs cycle Mitochondrial matrix Cytoplasm
ETC Mitochondrial inner membrane
Plasma membrane
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Pathway ATP produced NADH produced
FADH2 produced
Glycolysis 2 2 0
Intermediate step 0 2
Krebs cycle 2 6 2
Total 4 10 2
Energy produced from complete oxidation of one
glucose using aerobic respiration.
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PathwayBy substrate-
level phosphorylation
By oxidative phosphorylation
From NADH
From FADH
Glycolysis 2 6 0
Intermediate step 0 6
Krebs cycle 2 18 4
Total 4 30 4
ATP produced from complete oxidation of one glucose
using aerobic respiration.
36 ATPs are produced in eukaryotes.