MOHANA KRISHNA REDDY MUDIAM RESUME Principal Scientist & Associate Professor (AcSIR) Unit Leader, Chromatography Unit, Analytical Chemistry & Mass Spectrometry Department CSIR-Indian Institute of Chemical Technology, Tarnaka, Uppal Road, Hyderabad- 500 007 (Mobile) No: 91-9454985887; E-mail: mmudiam@iict.res.in; mmudiam8@gmail.com

HIGHLIGHTS

17 years of experience in analytical and bioanalytical chemistry Experience in mass spectrometry based metabolomics Qualified NABL Technical Assessor in Chemical Sciences Experience in maintaining GLP and/or NABL compliant analytical laboratory in the area of

bioanalytical, food, pharmaceutical, environmental and forensic analysis BOYSCAST Fellow in Medicinal Chemistry Young Scientist Award for best oral presentation at ICEHT-2012 held at Tirupati Experience in analytical method development & validation of toxicants/analytes/ metabolites in

pharmaceutical formulations, biological and food matrices Experience in the multistep organic synthesis of bioactive compounds Extensive skills in analytical characterization and data interpretation Hands-on experience on GC/MS/MS (Quantum XLS, Thermo Scientific), GC/MS (TurboMass, Perkin

Elmer), GC (Auto System XL and Claurus 500, Perkin Elmer), HPLC (LC-10AT, Shimadzu), HPTLC (CAMAG), CE (Prince Technologies), SPE, SPME (Supelco), SPPS (Protein Technologies), LC-ESI-MS (Esquire 3000 Plus, Bruker) and LC-QqQ-MS (API 4000, AB SCIEX)

Provided training to students and scientists of various organizations on major equipments like CE, GC, HPLC and GC-MS

Academic Editor, PLoSONE Editorial Board Member, Austin Journal of Analytical and Pharmaceutical Chemistry Experience in the area of Nano-Molecularly Imprinted Polymers (Nano MIPs)

EDUCATIONAL & PROFESSIONAL QUALIFICATIONS 15

th Oct-15

th Dec, 2014: Visiting Scientist in CSIRO Land & Water, Waite Campus, Urrbrae,

Adelaide, Australia. Research work: Method development for the quantitative determination of emerging pharmaceuticals and pollutants in environmental and biological samples using GC-MS/MS.

2007 – 2008: Visiting Scientist in Medicinal Chemistry, SKCCC at Johns Hopkins University, Baltimore, USA. Research work: α, β-Unsaturated Carbonyl System of Chalcone-based Derivatives are Responsible for Broad Inhibition of Proteasomal Activity and preferential killing of HPV positive cervical cancer cells.

2001–2005: Ph.D. in Biochemistry in Central Forensic Science Laboratory, (Osmania University),

Hyderabad, India. Thesis: Biochemical analysis of opium (Papaver somniferum L) latex and its application in source identification

1997-1999: M.Sc. in Organic Chemistry, Regional Engineering College (Now upgraded to National

Institute of Technology), Warangal, India. Dissertation work: Synthesis of 3-methoxy-4-methyl-benzoic acid methyl ester, National Chemical Laboratory, Council of Scientific and Industrial Research (CSIR), PUNE – 411 008, India.

1994-1997: B.Sc. in Maths, Phy, Chem from Hindu College, Guntur, Andhra Pradesh

PERSONAL PROFILE: Date of Birth: 25

th July, 1977

Spouse: Dr. Brunda Ganneru, Senior Scientist, BBIL, Shamirpet, Hyderabad Native place: Guntur, Andhra Pradesh

Mohana Krishna Reddy Mudiam

PROJECTS (In progress)

1. Identification and management of sewage effluent discharges to keep our rivers healthy: Comparative investigations in India and South Australia. Selected for funding under Australia-India Strategic Research Fund (AISRF) (DST-DIISRTE Research Project). (Principal Investigator@IICT), 2015-2017.

PROJECTS Completed

1. Evaluation of nanoparticle toxicity using mass spectrometry based comparative metabolomics in model organisms. GAP-270 (funded by DST, New Delhi) (23 lakhs for 3 years), (2013-16) (Principal Investigator)

2. Integrated NextGen Approaches in Health, Disease and Environmental Toxicity (INDEPTH). CSIR-Network Programme (2012-17) (Work Package Leader)

3. Nanomaterials: Applications and impact on Safety, Health and Environment (NanoSHE, BSC0112). CSIR-Network Programme (2012-17) (Co-Nodal Scientist till November, 2015)

4. Monitoring Ganga Water Quality. Sponsored by NEERI Nagpur under NMCG Programme, Government of India. Duration: May 2015 to November 2016. Project cost: Rs. 20, 42,400/-. (Co-PI)

5. All India Network Project on Pesticide Residue. Grand-in-Aid by ICAR, New Delhi (GAP-168) during 2007-2015 (18 lakhs/year) (Handed over to Dr. D K Patel @CSIR-IITR due to my transfer to CSIR-IICT) (PI)

6. Safe water for the future through Indo-Oz Network (AusAID Programme) sponsored by CSIRO Land & Water, Adelaide, Australia. Project cost: 30,000 AUD. Duration: March 2013 – February 2015. (Co-PI)

7. Migration testing of Phthalates and Metals, Sponsored by Him Jagriti, Uttaranchal Welfare Society, Dehradun, SSP-273, Cost: 4 lakhs, Period: 06 months (June-December 2014) (Co-PI)

8. Can hair metabolomics be used as a biomarker tool for pesticide exposure? Funded under CSIR-EMPOWER SCHEME (2010-2012) (20 lakhs) (PI)

9. Monitoring of pollution parameters by third party recognized by UPPCB. The project cost is 27.53 lakhs. The duration is 20-05-2013 to 31-08-2015. A project sponsored by M/s NTPC Limited, Rihandnagar, Sonebhadra, UP. (Co-PI)

10. Analysis of Atrazine, Pendimethalin and Butachlor in tissues, soil and water. A project completed. Funded by National Bureau of Fish Genetic Resources (SSP-248). (5.4 lakhs) (Co-PI).

11. Health risk assessment and environmental monitoring among pesticide sprayer in mango plantation of UP. Grand-in-Aid project by UPCST (GAP-169) (14 lakhs) during 2007-2010 (Project report submitted). (Co-PI)

12. Estimation of phthalates & metals in polypropylene (PP)/high density polyethylene (HDPE) sacks viz-a-vis jute bags for storage of food grains (wheat & rice) (SSP-152) during 2006-2010 (report submitted) (14 lakhs). (Co-PI)

13. Identification and quantification of polyaromatic hydrocarbons (PAHs) in soil and Gomati river sediments in Lucknow city. Ministry of Environment & Forests (MoEF), New Delhi during the period of 2005-2007. (8 lakhs) (GAP-142). (Co-PI)

Ph.D. FELLOWS (PRESENT)

1. Mr. Raju D, Manager, GVKBio, Hyderabad (AcSIR PhD programme) 2. Mr. Deviprasad R, Scientist, GVKBio, Hyderabad (AcSIR PhD programme) 3. Mr GNV Satyanarayana, Technical Assistant, CSIR-IITR 4. Mr. Hussain Shaik, UGC-JRF 5. Mr. Konda Satyanand, CSIR-JRF

Mohana Krishna R Mudiam

6. Mr. Amit Kumar Singh, Associate Scientist, GVKBio, Hyderabad 7. Mr. Manoj Kumar Gupta, UGC-SRF 8. Ms. Tejasvi Bhatia, UGC-SRF 9. Mr. Satyajeet Rai, Project Assistant (GAP-168, MPRNL, DAC Project)

Ph.D./Project FELLOWS (PAST)

1. Dr. Rajeev Jain, UGC Fellow, 2009-2014 2. Dr. Ratnasekhar, CSIR Fellow, 2010-2014 3. Dr. Abhishek Chauhan, UGC Fellow, 2010-2014 4. Dr. Vivek Pandey, BBUA Fellow, 2012-2016 5. Mr. Amit Kumar Singh, Project Assistant (GAP-270, SERB Project) (August 2013-Oct 2015) 6. Ms Pratibha Singh, Project Assistant (BSC0111, INDEPTH network project) (April 2013-Sep 2015) 7. Ms. Pathya Pandey, Project Fellow (BSC0111 INDEPTH network project) (April 2013-Jan 2016) 8. Mr. Abhishek Kumar Singh, Project Assistant (GAP-168, MPRNL, DAC Project) (Jan 2013-Oct

2015) 9. Mr. Gaurav Pal, Project Fellow (BSC0112, NanoSHE network project) (Oct 2014-Oct 2015) 10. Ms. Swapna Yadav, Project Assistant (GAP-168, MPRNL, DAC Project) (Jan 2014-Dec 2015) 11. Mr. Anshuman Srivastava, Project Assistant (GAP-168, MPRNL, DAC Project) (August 2013-Dec

2015)

EMPLOYMENT Dec 2015-Present: Principal Scientist, Analytical Chemistry & Mass Spectrometry (ACMS) Divison,

CSIR-Indian Institute of Chemical Technology, Hyderabad, INDIA.

Analytical method development validation using various analytical instruments for xenobiotics in various matrices of chemical, pharmaceutical, environmental, food, biological and agricultural interest

Mentoring the technical staff on the analytical method development and services on the instruments like HPLC, UHPLC, IEC, GPC, ICP-OES, FT-IR, CHNS Analyzer, HPTLC, Capillary Electrophoresis etc.

Providing analytical services to the Chemical Industries

Mass spectrometry based metabolomics for disease and toxicology applications

Establishment of Quality Infrastructure in Environmental Analytics for Chemical Measurements of Contaminants

Quantum dot coupled to Molecular Imprinting Polymers (MIPs) based sensors for the detection of xenobiotics and their metabolites

Sample preparation methods for the measurement of analytes in various matrices

Feb 2013-Nov, 2015: Principal Scientist in Analytical Chemistry Laboratory, CSIR-Indian Institute of

Toxicology Research, Lucknow, INDIA. Mainly focused on research in analytical chemistry, specifically,

Mass spectrometry based metabolomics after pesticide or nanoparticle exposure in model organisms

Establishment of Quality Infrastructure in Environmental Analytics for Chemical Measurements of Pesticides at IITR (Designated Institute, DI)

Development of novel Molecular Imprinting Polymers (MIPs) to be used as sample cleanup cartridges for chemical pollutants.

Development of New Chemical derivatization assays for the GC-MS analysis of environmental contaminants containing polar functional groups.

2009 – 2013: Senior Scientist in Analytical Chemistry Laboratory, CSIR-Indian Institute of Toxicology

Research, Lucknow, INDIA. Mainly focused on research in analytical chemistry, specifically,

Mass spectrometry based metabolomics after pesticide or nanoparticle exposure in model organisms

Establishment of Quality Infrastructure in Environmental Analytics for Chemical Measurements of Pesticides at IITR (Designated Institute, DI)

Development of novel Molecular Imprinting Polymers (MIPs) to be used as sample cleanup cartridges for chemical pollutants.

Development of New Chemical derivatization assays for the GC-MS analysis of environmental contaminants containing polar functional groups.

2005- 2009: Scientist in Analytical Chemistry, CSIR-Indian Institute of Toxicology Research,

Lucknow, INDIA.

Operation as well as application training to scientists/students, especially on GC, GC/MS.

Development/Standardization of analytical methods for GC-MS in Toxicology.

Mohana Krishna Reddy Mudiam

Characterization of low molecular weight aromatic compounds from microbial degradation of chemical pollutants and biopolymers by GC-MS.

Preparation and analyses of environmental samples for Pesticides, PAH's, VOC etc.

Full analytical characterization support to all the projects of the institute using GC/MS, GC's.

Prepared research proposals and coordinated the projects.

2000-2004 Research Fellow in Analytical Chemistry at Central Forensic Science

Laboratory, Hyderabad.

Development of analytical methods for the evaluation of toxicants and drugs of forensic interest in food and plant matrices by CE, HPLC, HPTLC, GC-MS.

1999-2000: Trainee chemist in Dr Reddy’s Research Foundation, a R&D unit of Dr. Reddy’s Laboratories, Hyderabad, INDIA.

Design and synthesis of second-generation oxazolidinones with a primary goal of identifying compounds with increased potency against Gram-positive as well as fastidious Gram-negative pathogens compared to Linezolid

Preparation of laboratories monthly reports and reporting to the Scientist-in-charge.

AWARDS AND FELLOWSHIPS Young Scientist Award for Best Oral Presentation (above 30 years category) for the paper

entitled “Gas chromatography-mass spectrometry based metabolomic approach for optimization and evaluation of earthworm sub-lethal responses to carbofuran” at International Conference on Environmental Impact on Human Health and Therapeutic Challenges (ICEHT-2012) and 6

th Annual Convention of Association of Biotechnology and Pharmacy (ABAP) held at Department

of Virology, Sri Venkateswara University, Tirupati during 20-22 December, 2012. Selected for Government of Ireland’s prestigious fellowship, “Irish Research Council for Science,

Engineering & Technology (IRCSET) postdoctoral fellowship scheme – 2008” in Medicinal Chemistry at Dublin City University, Dublin, Ireland.

Awarded one of the India’s prestigious fellowships “Better Opportunities for Young Scientists in the Chosen Area of Science and Technology (BOYSCAST)” (2006-2007) to do advanced research on the design, discovery and delivery of anticancer molecules at Sidney Kimmel Comprehensive Cancer Centre at Johns Hopkins, Baltimore, Maryland, USA from March 2007 – March, 2008.

Selected for YOUNG SCIENTIST AWARD presentation for the paper entitled “Biochemical analysis of opium (Papaver somniferum L) latex and its application in source identification” at 93

rd Indian

Science Congress organized by ANGRAU, Hyderabad on 3-7th January 2005.

Awarded Senior Research Fellowship at national level by Council of Scientific and Industrial

Research, New Delhi, Government of India, 2004.

Awarded Senior and Junior Research Fellowship at national level by Directorate of Forensic Science, New Delhi, India, 2000.

SKILLS & ACCOMPLISHMENTS

(A) ANALYTICAL More than 15 years of diverse analytical experience in the analysis of plant metabolites, toxicants,

drugs and chemical pollutants in various biological, food and environmental matrices.

Sample preparation and trace level analyses of volatiles, semi volatiles & pesticides in biological samples.

Extensively involved in several studies with instruments such as Ten years on GC/MS (Make: Perkin Elmer) for (i) Characterization of microbial degradation products of

Kraft Lignin (biopolymer), Pentachlorophenol’s (PCPs), Pesticides (ii) photo degradation of PAHs and (iii) identification of fatty acids (FAMEs) in opium latex and rat brain, (iv) Organochlorine and Organophosphorous pesticides in whole blood, serum, and sheep rumen samples (v) Pheromones in

Mohana Krishna R Mudiam

Buffalo urine samples and (vi) characterization of bio transformed metabolites of organochlorine and organophosphorous pesticides.

Three Years on Capillary Electrophoresis (Make: Prince Technologies) for the quantitative evaluation of principal opium (narcotic commodity) alkaloids in opium latex and Sanguinarine in argemone oil.

Three years on HPLC (Make: Shimadzu) for the analysis of diazepam in cream biscuits and chromatographic fingerprinting of amino acids in opium.

Three years on GC (Perkin Elmer) for the determination of fatty acids in opium latex and Organochlorine and Organophosphorous pesticides and their metabolites in placenta, blood and urine samples.

One year on LC- ESI-MS (Make: Bruker) for impurity profiling of peptides and chalcones.

One year on HPTLC (Make: Camag) for quantitative evaluation of diazepam in cream biscuits and Sanguinarine and Dihydrosanguinarine in argemone adulterated mustard oil.

One year on NMR for structural characterization of organic compounds.

Major contributions have been made in the creation of analytical facility for the evaluation of chemical pollutants and their biotransformed products using different microextraction techniques which includes, solid-phase microextraction (SPME) and dispersive liquid-liquid microextraction (DLLME) coupled with derivatization techniques for GC/MS analysis.

Hands on experience on various sample extraction techniques such as Liquid-Liquid Extraction (LLE), Solid Phase Extraction (SPE), SPME and DLLME. Familiar with Liquid Phase Microextraction (LPME).

Analytical method development, validation and writing SOP's for GC, GC/MS and other techniques.

Trained students and various faculty members on GC, GC/MS, HPLC and other laboratory equipment.

Familiar with chemometric analysis of analytical data.

Familiar with NABL accreditation programme in chemical analysis

Got 5th Rank in Chemistry of ANUPGCET-1997 and 8

th Rank in Chemistry of AUPGCET-1997.

(B) ORGANIC SYNTHESIS

Extensive experience in multi-step organic synthesis and solid phase peptide synthesis and

purifications by preparative HPLC and characterization of complex molecules. Specific work includes: Design, synthesis and biological evaluation of candidate proteasome inhibitors that prevent E6

mediated p53 degradation and selectively kill HPV+ Cervical Cancer Cells.

Design and synthesis of second-generation oxazolidinones with a primary goal of identifying compounds with increased potency against Gram-positive as well as fastidious Gram-negative pathogens.

Synthesis of peptides using Solid Phase Peptide Synthesizer (SPPS) and their coupling to bioactive moieties.

Synthesis and scale up of 3-methoxy-4-methyl benzoic acid, a starting material for the synthesis of the drug, viz., Zafirlukast

(C) OTHER SKILLS

Acting referee to reputed international journals viz., (i) Journal of Chromatography A (ii) Journal of

Chromatography B, (iii) Water Environment Research (iv) Talanta, (v) Journal of AOAC international and (vi) Science of the Total Environment (vii) Analytical and Bioanalytical Chemistry

Adjudicated 20 Ph.D. theses in Chemistry

Writing research reports, manuscripts and final project reports

Exposure to Intellectual Property Rights and WTO Related Issues

Exposure to Laboratory Quality Management and Internal Audit as per ISO/IEC 17025:2005

Familiar with Measurement of Uncertainty in Chemical Analysis by Chromatography and Mass spectrometry

Mohana Krishna Reddy Mudiam

Capable of (i) Doing collaborative and independent research, (ii) Maintaining good interpersonal

relationships AND (iii) Identify experimental problems and resolve them independently.

Computer software: MS Word, PowerPoint, Excel, Chemdraw, Microcal Origin, Statgraphics and SciFinder

Exploration of new reagents for organic transformations and maintain up-to-date information on analytical advancements

Actively involved as organizing member for various international/national conferences and training programmes held in India

TRAINING/STUDY PROGRAMMES ATTENDED

Attended refresher course on “Processing and Analytical Methodologies of Oils & Fats” held at Centre for

Lipid Research, CSIR-Indian Institute of Chemical Technology, Hyderabad during 25-27, February, 2015.

Successfully completed a Basic operator training on LC-MS/MS system held at the AB SCIEX, Centre of

Excellence, DHR Holding India Pvt. Ltd., Gurgoan on 28-30th January 2013.

Attended one day symposium on Principles of Good Laboratory Practices at CSIR-IITR, Lucknow on 4th

December 2012 organized by STOX, India & CSIR-IITR.

Study tour on “Strengthening the quality infrastructure in environmental analytics” in Germany and Attended CCQM Organic Analysis Working Group meeting at Paris from April 2 – 13, 2011. Visited various institutes in Germany to understand their set up in “Metrology in Chemistry (MiC)”.

Training Programme on ISO/IEC 17025 NABL Assessor’s Training course organized at Indian Institute of Quality Management (IIQM), ETDC, Jaipur 21– 25

th February 2011. Qualified as NABL Technical Assessor in

Chemical Sciences.

Attended Indo-German Workshop on “Metrology in Chemistry (MiC) from 6-10th

December 2010 at Mysore.

Training Programme on Laboratory Management & Internal Audit as per ISO/IEC 17025:2005 organized at

CETE, Bangalore during March 2-5, 2009.

TRAINING PROGRAMMES ORGANIZED/INVOLVED Actively involved in organizing various training programmes as listed below

Training Programme on Analysis of Pesticides and other organic chemicals in environmental samples

sponsored by Central Pollution Control Board (CPCB), Delhi during October 20-24, 2008 at Indian Institute of Toxicology Research, Lucknow.

Training Programme on Analysis of toxic metals in environmental samples sponsored by Central Pollution

Control Board (CPCB), Delhi during September 11-13, 2008 at Indian Institute of Toxicology Research, Lucknow.

Training Programme on Mercury Management in FL lamps sponsored by Central Pollution Control Board

(CPCB), Delhi during September 6-8, 2008 at Indian Institute of Toxicology Research, Lucknow.

Training Programme on Concept and Application of Advanced Instrumentation Monitoring: GC-MS, GLC, HPLC, AAS, ICP, TCLP and Air Monitoring Instruments) sponsored by Central Pollution Control

Board (CPCB), Delhi during July 21-25, 2008 at Indian Institute of Toxicology Research, Lucknow.

Training Programme on “Analysis of pesticides and other organics – SELA 2” sponsored by Central

Pollution Control Board (CPCB), Delhi during July 21-25, 2006 at Indian Institute of Toxicology Research, Lucknow.

Demonstrated GC-MS and delivered a talk on Usage of Solid Phase Micro extraction (SPME) as a

novel sample cleanup technique in three of the above training programmes. MEMBER IN COMMITTEES/INVITED FACULTY

Scientific Panel Member on Contaminants in Food Chain at FSSAI (2017-2020)

Co-coordinator, AcSIR Chemical Sciences at CSIR-IITR, Lucknow (2011-2015)

Area Co-coordinator, Nanotherapeutics and Nanotoxicology at CSIR-IITR, Lucknow (2014-15)

Mohana Krishna R Mudiam

Member, Core Academic Committee on AcSIR at CSIR-IITR, Lucknow (2011-2015)

Member, JRF Screening and selection committee for AcSIR course in Chemical Sciences (2011-2015)

Member, Formulation Analysis Unit, Vishaktata Pareekshan – GLP Anuroop Suvidha at CSIR-IITR, Lucknow. (2013-15)

Member, Project Evaluation Committee at CSIR-IITR for 2012-13.

Member of Expert Group for Evaluation of Schemes at LNJN National Institute of Criminology and Forensic Science, Ministry of Home Affairs, GOI, Outer Ring Road, Sector-3, Rohini, Delhi for the year 2010-2011.

Invited Faculty for M.Sc. Forensic Science with specialization in Forensic Chemistry and Toxicology in Institute of Forensic Science, Gujarat Forensic Sciences University, Gandhinagar, Gujarat.

Member in Hi Power Purchase Committee, Biotech Park, Lucknow, UP for 2006-07

PROFESSIONAL AFFILIATIONS Indian Society for Mass Spectrometry (ISMAS), India The International Association of Forensic Toxicologists Indian Society for Analytical Scientists-Delhi Chapter Society for Toxicology (STOX), India

PUBLICATIONS 1. Satyajeet Rai, Madhuri Devi Gullapalli, Anshuman Srivastava, Hussain Shaik, Mohammed Haris Siddiqui,

Mohana Krishna Reddy Mudiam*. A quick and rapid method for the quantitative determination of 34 pesticides in non-alcoholic carbonated beverages using liquid-liquid extraction coupled to dispersive solid-phase cleanup followed by gas chromatography-tandem mass spectrometry. Journal of AOAC International (In press), 2017. (IF: 0.918)

2. Sachin Tripathi, Rajesh Kushwaha, Juhi Mishra, Manoj Kumar Gupta, Harish Kumar, Somali Sanyal, Dhirendra Singh, Sabyasachi Sanyal, Amogh Anant Sahasrabuddhe, Mohan Kamthan, Mohana Krishna Reddy Mudiam, Sanghamitra Bandyopadhyay. Docosahexaenoic acid up-regulates both PI3K/AKT-

dependent FABP7-PPAR interaction and MKP3 that enhance GFAP in developing rat brain astrocytes. Journal of Neurochemistry 2017, 140(1), 96-113. (IF: 3.842).

3. Sanjay Mishra, Girish Rai, Amit Kumar Srivastava, Amit Kumar Singh, Asrar Ahmad Shankar Suman, Madhulika Singh, Mohana Krishna Reddy Mudiam, Brij Nath Tiwari, Prachi Gupta, Vivek Kumar Gaur, Vikas Srivastava, Vijay Kumar, Yogeshwer Shukla. miR-34a overexpression induces senescence associated secretory phenotypes in doxorubicin induced pseudo-senescent triple negative breast cancer cells via SOCS4-IL6-STAT3 axis. American Journal of Cancer Research, 2016, 6(10), XXX (In press, corrected proof). (IF: 3.425).

4. Smita Kumari, Raj Kumar Regar, Abhay Bajaj, Ratnasekhar Ch, Gubbala Naga Venkata Satyanarayana, Mohana Krishna Reddy Mudiam, Natesan Manickam. Simultaneous biodegradation of polyaromatic hydrocarbons by a Stenotrophomonas sp: Characterization of nid genes and effect of surfactants on degradation. Indian Journal of Microbiology, 2017, 57(1), 60-67. (IF: 1.143).

5. Madhuree Kumari, Aradhana Mishra, Shipra Pandey, Satyendra Pratap Singh, Vasvi Chaudhry, Mohana

Krishna Reddy Mudiam, Shatrunajay Shukla, Poonam Kakkar, Chandra Shekhar Nautiyal. Physico-chemical condition optimization during biosynthesis leads to development of improved and catalytically efficient gold nano particles. Scientific Reports, 2016, 6:27575, DOI: 10.1038/srep27575. (IF: 5.228).

6. Anshuman Singh, Anubha Mudawal, Pratibha, Rajeev Jain, Saumya Naira, Rajendra K. Shukla, Sanjay Yadav, Dhirendra Singh, Vinay Kumar Khanna, Rajnish Kumar Chaturvedi, Mohana K.R. Mudiam, Rao Sethumadhavan, Mohammad Imran Siddiqi and Devendra Parmar. Prenatal exposure of cypermethrin induces similar alterations in xenobiotic metabolizing cytochrome P450s and rate limiting enzymes of neurotransmitter synthesis in brain regions of rat offspring’s during postnatal development. Molecular Neurobiology, 2016, 53(6), 3670-3689. (IF: 5.397)

7. Tejasvi Bhatia, Manoj Kumar Gupta, Pratibha Singh, Abhishek Chauhan, Prem Narain Saxena, Mohana Krishna Reddy Mudiam*. Sol-gel based synthetic approach for extracting highly profound and versatile drug aspirin and its metabolites in urine followed by GC-MS/MS. Bioanalysis, 2016, 8(8), 795-805. (IF: 2.813)

Mohana Krishna Reddy Mudiam

8. Satyajeet Rai, Abhishek Kumar Singh, Anshuman Srivastava, Sapna Yadav, Mohd. Haris Siddiqui,

Mohana Krishna Reddy Mudiam*. Comparative evaluation of QuEChERS method coupled to DLLME extraction for the analysis of multiresidue pesticides in vegetables and fruits by gas chromatography-mass spectrometry. Food Analytical Methods, 2016, 9(9), 2656-2669. (IF: 2.167)

9. Amarnath Singh, Ritul Kamal, Mohana Krishna Reddy Mudiam, MK Gupta, GNV Satyanarayana, Vipin

Bihari, Nishi Shukla, Altaf Hussain Khan, Chandrasekhran Nair Kesavachandran. Heat and PAHs emissions in Indoor kitchen air and its impact on kidney dysfunctions among kitchen workers in Lucknow, North India. PLOS ONE, 2016, 11(2), e0148641. (IF: 3.057)

10. Arvind Kumar Shukla, Ch Ratnasekhar, Prakash Pragya, Hitesh Singh Chaouhan, Devendra Kumar Patel, Debapratim Kar Chowdhuri, Mohana Krishna Reddy Mudiam*. Metabolomic analysis provides insights on paraquat induced Parkinson-like symptoms in Drosophila Melanogaster. Molecular Neurobiology, 2016, 53(1), 254-269. (IF: 5.397)

11. Shagufta Khan, Tejasvi Bhatia, Purushottam Trivedi, GNV Satyanarayana, Kapil Mandrah, Prem Narayan

Saxena, Mohana Krishna Reddy Mudiam, Somendu Kumar Roy. Selective solid-phase extraction using molecularly imprinted polymer as a sorbent for the analysis of fenarimol in food samples. Food Chemistry, 2016, 199, 870-875 (IF: 4.052)

12. Amarnath Singh, Chandrasekharan Nair Kesavachandran, Ritul Kamal, Vipin Bihari, Manoj Kumar Gupta,

Mohana Krishna Reddy Mudiam, Gubbala Naga Venkata Satyanarayana, Abhay Raj, Izharul Haq, Nishi Kumar Shukla, Altaf Husain Khan, Anup Kumar Srivastava. Assessing hazardous risks of indoor airborne polycyclic aromatic hydrocarbons in the kitchen and its association with lung functions and urinary PAH metabolites in kitchen workers. Clinica Chimica Acta, 2016, 452, 204-213. (IF: 2.799)

13. Ratnasekhar Ch, Amit Kumar Singh, Pathya Pandey, Prem Narain Saxena, Mohana Krishna Reddy

Mudiam*. Identifying the metabolic perturbations in earthworm induced by cypermethrin using gas chromatography-mass spectrometry based metabolomics. Scientific Reports, 2015, 5:15674. (IF: 5.228)

14. Ratnasekhar Ch, Madavi Sonane, Aruna Satish, Mohana Krishna Reddy Mudiam*. Metabolomics

reveals the perturbations in the metabolome of Caenorhabditis elegans exposed to titanium dioxide nanoparticles. Nanotoxicology, 2015, 9(8), 994-1004. (IF: 7.913)

15. Rajeev Jain, Manoj Kumar Gupta, Abhishek Chauhan, Vivek Pandey, Mohana Krishna Reddy Mudiam*.

Ultrasound assisted DLLME followed by GC-MS/MS analysis for the determination of valproic acid in urine samples. Bioanalysis, 2015, 7(19), 2451-2459. (IF: 2.813)

16. Abhishek Chauhan, Tejasvi Bhatia, Manoj Kumar Gupta, Pathya Pandey, Vivek Pandey, Prem Narain Saxena, Mohana Krishna Reddy Mudiam*. Imprinted nanospheres based on precipitation polymerization for the simultaneous extraction of six urinary benzene metabolites from urine followed by injector port silylation and gas chromatography-tandem mass spectrometric analysis. Journal of Chromatography B, 2015, 1001, 66-74. (IF: 2.729)

17. Vivek Pandey, Abhishek Chauhan, Gajanan Pandey, Mohana Krishna Reddy Mudiam*. Optical sensing

of 3-phenoxybenzoic acid as a pyrethroid pesticides exposure marker by surface imprinting polymer capped on manganese-doped zinc sulfide quantum dots. Analytical Chemistry Research, 2015, 5, 21-27. (IF: NA).

18. Vivek Pandey, Gajanan Pandey, Vinay Kumar Tripathi, Sapna Yadav, Mohana Krishna Reddy

Mudiam*. Nucleation temperature controlled synthesis and in-vitro toxicity evaluation of L-Cysteine capped Mn: ZnS quantum dots for intracellular imaging. Luminescence: The Journal of Biological and Chemical Luminescence, (In press), 2015. (IF: 1.518)

19. Gati Krushna Panigrahi, Ratnasekhar Ch, Mohana K R Mudiam, Vipin M Vashishtha, S Raisuddin, Mukul

Das. Activity guided chemo toxic profiling of Cassia Occidentalis (CO) seeds: Detection of toxic compounds in body fluids of CO exposed patients and experimental rats. Chemical Research in Toxicology, 2015, 28(6), 1120-1132. (IF: 3.529)

20. Meenu Varshney, Abhijit Chandra, Rajeev Jain, Riaz Ahmad, Vipin Bihari, C Keshavachandran, Mohana

KR Mudiam, Satyakam Patnaik, S K Goel. Occupational health hazrds of trichloroethylene among

Mohana Krishna R Mudiam

workers in relation to altered mRNA expression of cell cycle regulating genes (p53, p21, bax and bcl-2) and PPARA. Toxicology Reports, 2015, 2, 748-757. (IF: NA)

21. Manoj Kumar Gupta, Rajeev Jain, Ratnasekhar Ch, Mohana Krishna Reddy Mudiam*. Determination of

polyaromatic hydrocarbon metabolites using dispersive liquid-liquid microextraction hyphenated to injector port silylation and gas chromatography-tandem mass spectrometry. Journal of Analytical Toxicology, 2015, 39(5), 365-373. (IF: 2.858).

22. Abhishek Chauhan, Tejasvi Bhatia, Amarnath Singh, Prem Narain Saxena, C Kesavchandran, Mohana

Krishna Reddy Mudiam*. Application of nanosized multi-template imprinted polymer for simultaneous extraction of polycyclic aromatic hydrocarbon metabolites in urine samples followed by ultra high performance liquid chromatography analysis coupled with fluorescent detector. Journal of Chromatography B, 2015, 985, 110-118. (IF: 2.729)

23. Syed Faiz Mujtabla, Ashish Dwivedi, Neera Yadav, Ratnasekhar Ch, Hari Narayan Kushwaha, Mohana

KR Mudiam, Gajendra Singh, Ratan S Ray. Superoxide mediated photomodification and DNA damage induced apoptosis by Benz(a)anthracene via mitochondrial mediated pathway. Journal of Photochemistry and Photobiology B, 2015, 142, 92-102. (IF: 2.960)

24. Snigdha Misra, Ajay Kumar, Ch Ratnasekhar, Vandana Sharma, Mohana Krishna Reddy Mudiam,

Kristipati Ravi Ram. Exposure to endosulfan influences sperm competition in Drosophila Melanogaster. Scientific Reports, 2014, 4, 7433; DOI: 10.1038/srep07433. (IF: 5.228)

25. Priyanka Bhatnagar, Soma Patnaik, Amit K Srivastava, Mohana KR Mudiam, Yogeshwer Shukla, Amulya

K Panda, Aditya B Pant, Pradeep Kumar, Kailash C Gupta. Anti-Cancer Activity of Bromelain Nanoparticles by Oral Administration. Journal of Biomedical Nanotechnology, 2014, 10, 3558-3575. (IF: 5.338)

26. Snigdha Mishra, Vandana Sharma, Anshuman Singh, Ratnasekhar Ch, Mohana Krishna Reddy Mudiam, Ravi Ram Kristipati. Identification of Drosophila based endpoints for the assessment and understanding of xenobiotic mediated male reproductive adversities. Toxicological Sciences 2014, 141(1), 278-291. (IF: 3.880)

27. Abhay Bajaj, Shanmugam Mayilraj, Mohana Krishna Reddy Mudiam, Devendra Kumar Patel, Natesan Manickam. Isolation and functional analysis of a glycolipid producing Rhodococcus sp strain IITR03 with potential for degradation of 1, 1, 1-trichloro-2,2-bis(4-chlorophenyl)ethane (DDT). Bioresource Technology, 2014, 167, 398-406. (IF: 4.917)

28. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Anshuman Singh, Haider A. Khan, Devendra Parmar.

Development of ultrasound assisted-dispersive liquid-liquid microextraction-large volume injection-gas chromatography-tandem mass spectrometric method for the determination of pyrethroid metabolites in the brain of cypermethrin treated rats. Forensic Toxicology, 2014, 32(1), 19-29. (IF: 5.756)

29. Ashutosh K Srivastava, Satyajeet Rai, M K Srivastava, M Lohani, M.K.R. Mudiam, LP Srivastava.

Determination of 17 organophosphate pesticide residues in mango by modified QuEChERS extraction method using GC-NPD/GC-MS and hazard index estimation in Lucknow, India. PLoSONE, 2014, 9(5): e96493. doi:10.1371/journal.pone.0096493. (IF: 3.057)

30. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Ritu Singh. Application of ultrasound-assisted dispersive

liquid-liquid microextraction and automated in-port silylation for the simultaneous determination of phenolic endocrine disruptor chemicals in water samples by gas chromatography–triple quadrupole mass spectrometry. Analytical Methods, 2014, 6, 1802-1810. (IF: 1.915)

31. Mohana Krishna Reddy Mudiam*, Abhishek Chauhan, Rajeev Jain, Yogesh Kumar Dhuriya, Prem Narain Saxena, Vinay Kumar Khanna. Molecularly imprinted polymer coupled with dispersive liquid-liquid microextraction and injector port silylation: A novel approach for the determination of 3-phenoxy benzoic acid in complex biological samples using gas chromatography-tandem mass spectrometry. Journal of Chromatography B, 2014, 945-946, 23-30. (IF: 2.729)

32. Mohana Krishna Reddy Mudiam*, Ratnasekhar Ch, Prem Narain Saxena. Gas chromatography-mass spectrometry based metabolomic approach for optimization and toxicity evaluation of earthworm sub-lethal responses to carbofuran. PLoS ONE, 2013, 8(12), e81077. (IF: 3.534), doi:10.1371/journal.pone.0081077. (IF: 3.057)

Mohana Krishna Reddy Mudiam

33. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Ratnasekhar Ch, Abhishek Chauhan, Haider A Khan, R C Murthy. Ultrasound assisted dispersive liquid-liquid microextraction followed by injector port silylation: A novel method for rapid determination of quinine in urine by gas chromatography-mass spectrometry. Bioanalysis, 2013, 5(18), 2277-2286. (IF: 2.813)

34. Deepak Gurbani, Santosh Kumar Bharti, Ashutosh Kumar, Alok K Pandey, Godson R.E.E. Ana, Ambrish

Verma, Devendra K. Patel, M.K.R. Mudiam, Swatantra K. Jain, Raja Roy, Alok Dhawan. Polycyclic aromatic hydrocarbons and their quinines modulate the metabolic profile and induce DNA damage in human alveolar and bronchiolar cells. International Journal of Hygiene and Environmental Health, 2013, 216(5), 553-565. (IF: 3.829)

35. Ritu Singh, Natesan Manickam, Mohana Krishna Reddy Mudiam, Ramesh Chandra Murthy, Virendra

Misra. An Integrated (Nano-Bio) Technique for Degradation of γ-HCH Contaminated Soil. Journal of Hazardous Materials, 2013, 258-259, 35-41. (IF: (4.836)

36. Mohana Krishna Reddy Mudiam*, Ratnasekhar Ch. Ultra sound assisted one step rapid derivatization

and dispersive liquid-liquid microextraction followed by gas chromatography-mass spectrometric determination of amino acids in complex matrices. Journal of Chromatography A, 2013, 1291, 10-18. (IF: 3.926)

37. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Abhishek Chauhan, Ratnasekhar Ch, R C Murthy, Haider A Khan. Simultaneous derivatization and preconcentration of parabens in food and other matrices by isobutyl chloroformate and dispersive liquid-liquid microextraction followed by gas chromatographic analysis. Food Chemistry, 2013, 141(1), 436-443. (IF: 4.052)

38. Mohana Krishna Reddy Mudiam*, Abhishek Chauhan, Amit Kumar Singh, V P Sharma, Prem Narain Saxena. Molecularly imprinted solid-phase extraction combined with dispersive liquid-liquid microextraction for selective preconcentration and liquid chromatographic determination of telmisartan in rat plasma, urine and pharmaceutical formulations. Bioanalysis, 2013, 5(7) 847-858. (IF: 2.813)

39. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Meenu Varshney, Ratnasekhar Ch, Abhishek Chauhan, Sudhir Kumar Goyal, Haider A Khan, R.C. Murthy. In matrix derivatization of trichloroethylene metabolites in human plasma with methyl chloroformate and their determination by solid-phase microextraction-gas chromatography-electron capture detector. Journal of Chromatography B, 2013, 925, 63-69. (IF: 2.729)

40. Mohana Krishna Reddy Mudiam*, Abhishek Chauhan, Krishna P Singh, Shailendra K Gupta, Rajeev

Jain, Ratnasekhar Ch, R C Murthy. Determination of t,t-muconic acid in urine samples using molecular imprinted polymer combined with simultaneous ethyl chloroformate derivatization and preconcentration by dispersive liquid-liquid microextraction. Analytical and Bioanalytical Chemistry, 2013, 405(1), 341-349. (IF: 3.125)

41. Mohana Krishna Reddy Mudiam*, Ratnasekhar Ch, Abhishek Chauhan, Natesan Manickam, Rajeev

Jain, R C Murthy. Optimization of ultransound assisted dispersive liquid-liquid microextraction by experimental design methodologies for the simultaneous determination of endosulfan and its metabolites in soil and urine samples by gas chromatography-mass spectrometry. Analytical Methods, 2012, 4, 3855-3863 (IF: 1.915)

42. Srivastava Ashutosh K, Srivastava M.K., Patel D.K., Mudiam M.K.R and Srivastava L.P. Gas-chromatographic determination of imidacloprid in water. Journal of Environmental Research and Development, 2012, 7(2), 643-651. (IF: NA)

43. Mohana Krishna Reddy Mudiam*, Ratnasekhar Ch, Rajeev Jain, Prem Narain Saxena, Abhishek

Chauhan, R C Murthy. Rapid and simultaneous determination of twenty amino acids in complex biological and food samples by solid-phase microextraction and gas chromatography-mass spectrometry with the aid of experimental design after ethyl chloroformate derivatization. Journal of Chromatography B, 2012, 907, 56-64 (IF: 2.729)

44. Ritu Singh, Virendra Misra*, Mohana Krishna Reddy Mudiam*, Lalit Kumar, Singh Chauhan, Rana

Pratap Singh. Degradation of γ-HCH spiked soil using stabilized Pd/Fe0 bimetallic nanoparticles:

Pathways, kinetics and effect of reaction conditions. Journal of Hazardous Materials, 2012, 237-238, 355-364. (IF: 4.836) (*Co-corresponding author)

45. Mohana Krishna Reddy Mudiam

*, Abhishek Chauhan, Rajeev Jain, Ratnasekhar Ch, Ekta Malhotra, R

C Murthy. Development, validation and comparison of two microextraction techniques for the rapid and

Mohana Krishna R Mudiam

sensitive determination of pregabalin in urine and pharmaceutical formulations after ethyl chloroformate derivatization followed by gas chromatography-mass spectrometric analysis. Journal of Pharmaceutical and Biomedical Analysis, 2012, 70, 310-319 (IF: 2.979)

46. Mohana Krishna Reddy Mudiam*, Rajeev Jain, Shailendra Kumar Maurya, Haider A Khan, Sanghamitra

Bandyopadhyay, R C Murthy. Low density solvent based dispersive liquid-liquid microextraction with gas chromatography-electron capture detection for the determination of cypermethrin in tissues and blood of cypermethrin treated rats. Journal of Chromatography B, 2012, 895-896:65-70. (IF: 2.729)

47. Mohana Krishna R Mudiam, S P Pathak, K Gopal, R C Murthy. Studies on urban drinking water quality in a tropical zone. Environmental Monitoring and Assessment, 2012, 184, 461-469. (IF: 1.679)

48. Shailendra Kumar Maurya, Asit Rai, Nagendra Kumar Rai, Shreekant Deshpande, Rajeev Jain, Mohana

Krishna Reddy Mudiam, Yena mandra, S Prabhakar, Sanghamitra Bandyopadhyay. Cypermethrin induces astrocyte apoptosis by the disruption of the autocrine/paracrine mode of epidermal growth factor receptor signaling. Toxicological Sciences, 2012, 125(2), 473-487. (IF: 3.854)

49. Mohana Krishna Reddy Mudiam

*, Rajeev Jain, Virendra K Dua, Amit Kumar Singh, V P Sharma, R C

Murthy. Application of Ethyl chloroformate derivatization for SPME/GC-MS determination of Bisphenol A in water and milk samples. Analytical and Bioanalytical Chemistry, 2011, 401(5), 1699-1701. (IF: 3.125)

50. Martina Bazzaro, Mohana Krishna R Mudiam

+, Ravi K Anchoori

+, Olga Issaenko, Srinivas Kumar,

Balasubramanyam Karanam, Zhenhua Lin, Rachel Isaksson Vogel, Riccardo Gavioli, Federica Destro, Valeria Ferretti, Richard BS Roden and Saeed R Khan. α,β-Unsaturated Carbonyl System of Chalcone-based Derivatives is Responsible for Broad Inhibition of Proteasomal Activity and Preferential Killing of HPV Positive Cervical Cancer Cells. Journal of Medicinal Chemistry 2011, 54(2), 449-456. (IF: 5.447)

51. S F Mujtaba, A Dwivedi, M K R Mudiam, D Ali, N Yadav, R S Ray. Production of ROS by Photozensitized

Anthracene Under Sunlight and UV-R at Ambient Environmental Intensities. Photo Chemistry and Photo Biology, 2011, 87, 1067-1076. (IF: 2.684)

52. Mahendra P Singh, M Mishra, A Sharma, AK Shukla, M K R Mudiam, DK Patel, K Ravi Ram, D Kar Chowdhuri. Genotoxicity and apoptosis in Drosophila melanogaster exposed to benzene, toluene and xylene: Attenuation by quercetin and curcumin. Toxicology and Applied Pharmacology, 2011, 253(1), 14-30. (IF: 3.705)

53. Manoj Kumar Pathak, Mohammad Fareed, Vipin Bihari, Mudiam Mohana Krishna Reddy, Devendra

Kumar Patel, Neeraj Mathur, Mohammad Kuddus, Chandrasekharan Nair Kesavachandran. Neurotoxic manifestation of occupational exposure to mixed pesticidews among sprayers in a mango plantation in India. Toxicological and Environmental Chemistry, 2011, 93(1), 188-196. (IF: 0.825)

54. Mohammad Fareed, Manoj Kumar Pathak, Vipin Bhari, Mohana Krishna Reddy Mudiam, Devendra

Kumar Patel, Neeraj Mathur, Mohammed Kuddus, Chandrasekharan Nair Kesavachandran. Hematological and Biochemical alternations in sprayers occupationally exposed to mixture of pesticides in a mango plantation at Lucknow, North India. Toxicological and Environmental Chemistry, 2010, 92(10), 1919-1928. (IF: 0.825)

55. M K R Mudiam

+, C Kesavachandran

+. Adverse sub-clinical health effects in pesticide exposed population:

Challenges and need for developing non-invasive clinical biomarkers. Current Science, 2010, 99(12), 1643. (IF: 0.926)

56. A Bajaj, A Pathak, M.R. Mudiam, S Mayilraj and N Manickam. Isolation and characterization of a

Pseudomonas sp strain IITR01 capable of degrading α-endosulfan and Endosulfan sulphate. Journal of Applied Microbiology, 2010, 109(6), 2135-43. (IF: 2.479)

57. Jitendra Nath Tiwari, Mohana Krishna Reddy Mudiam, Devendra Kumar Patel, Sudhir Kumar Jain,

Ramesh Chandra Murthy and Natesan Manickam. Isolation of Pyrene degrading Achromobacter xylooxidans and Characterization of Metabolic Products. World Journal of Microbiology and Biotechnology, 2010, 26, 1727-1733. (IF: 1.779)

58. Mohana Krishna Reddy Mudiam

*, Mahendra Pratap Singh, D.Kar Chowdhuri, R C Murthy. Quantitative

evaluation of Benzene, Toluene and Xylenes in the Larvae of Drosophila melanogaster by Solid Phase

Mohana Krishna Reddy Mudiam

Microextraction/Gas Chromatography-Mass Spectrometry for potential used in Toxicological Studies. Journal of AOAC International, 2010, 93(5), 1595-1599. (IF: 0.918)

59. Ranu Tripathi, Rakesh Kumar, Mohana Krishna Reddy Mudiam, D K Patel and Jai Raj Behari. Distribution, Sources and Characterization of Polycyclic Aromatic Hydrocarbons in the Sediment of the River Gomti, Lucknow, India. Bulletin of Environmental Contamination and Toxicology 2009, 83(3), 449-454. (IF: 1.255)

60. Rohit A Sinha, Priyanka Khare, Asit Rai, Shailendra Kumar Maurya, Amrita Pathak, Vishwa Mohan, Geeta

Kumar Nagar, Mohana Krishna Reddy Mudiam, Madan M Godbole and Sanghamitra Bandyopadhyay. Anti-apoptotic role of omega 3 fatty acids in developing brain: perinatal hypothyroid rat cerebellum as apoptotic model. International Journal of Developmental Neuroscience 2009, 27(4), 377-383. (IF: 2.580)

61. Mahendra Pratap Singh, M.M. Krishna Reddy, N. Mathur, D.K. Saxena and D. Kar Chowdhuri. Induction

of hsp70, hsp60, hsp83 and hsp26 and oxidative stress markers in benzene, toluene and xylene exposed Drosophila melanogaster: Role of ROS generation. Toxicology and Applied Pharmacology, 2009, 235(2), 226-243. (IF: 3.705)

62. Ram Chandra, Shail Singh, M M Krishna Reddy, D K Patel, Hemant J. Purohit and Atya Kapley. Isolation

and characterization of bacterial strains Paenibacillus sp. and Bacillus sp. for kraft lignin decolorization from pulp paper mill waste. Journal of General and Applied Microbiology, 2008, 54, 399-407. (IF: 0.943)

63. Vipul Kumar Singh, Jyoti, Madhu Anand, Prabhat Kumar, Devendra Kumar Patel, Mudiam Mohana

Krishna Reddy, Mohammed Kaleem Javed Siddiqui. Comparision of polycyclic aromatic hydrocarbon levels in placental tissues of Indian women with full and preterm deliveries. International Journal of Hygiene and Environmental Health, 2008, 211(5-6), 631-647. (IF: 3.829)

64. Shail Singh, R Chandra, D K Patel, M M K Reddy, Vibhuti Rai. Investigation of the biotransformation of

pentachlorophenol and pulp paper mill effluent decolorisation by the bacterial strains in a mixed culture. Bioresource Technology, 2008, 99(13), 5703-5709. (IF: 4.917)

65. N Manickam, M K Reddy, H S Saini and R Shanker. Isolation of hexachlorocyclohexane-degrading Sphingomonas sp. By dehalogenase assay and characterization of genes involved in γ-HCH degradation. Journal of Applied Microbiology, 2008, 104, 952-960. (IF: 2.479)

66. Abhay Raj, M M Krishna Reddy, Ram Chandra, Hemant J Purohit, Atya Kapley. Biodegradation of kraft-lignin by Bacillus sp. Isolated from paper mill sludge. Biodegradation, 2007, 18, 783 – 792. (IF: 2.336)

67. Abhay Raj, Ram Chandra, M M Krishna Reddy, Hemant J Purohit, Atya Kapley. Biodegradation of kraft lignin by newly isolated bacterial strain. Aneurinibacillus aneurinilyticus from sludge of pulp paper mill. World Journal of Microbiology and Biotechnology, 2007, 23, 793-799.(IF: 1.779)

68. Abhay Raj, M M Krishna Reddy, Ram Chandra. Identification of low molecular weight aromatic

compounds by gas chromatography – mass spectrometry (GC-MS) from kraft lignin degradation by three Bacillus sp. International Biodeterioration & Biodegradation, 2007, 59, 292-296. (IF: 2.131)

69. Abhay Raj, M M Krishna Reddy, Ram Chandra. Decolourisation and treatment of pulp and paper mill

effluent by lignin degrading Bacillus sp. Journal of Chemical Technology & Biotechnology, 2007, 82, 399-406 (IF: 2.349)

70. Vipul K. Singh, Jyoti, M M Krishna Reddy, C. Keshavchandran, S.K. Rastogi, M.K.J. Siddiqui.

Biomonitoring of organochlorines, glutathione, lipid peroxidation status and cholinesterase activity among pesticide sprayers in mango orchards. Clinica Chimica Acta, 2007, 377(1-2), 268-272. (IF: 2.799)

71. C. Kesavachandran, Vipul K.Singh, N. Mathur, S.K. Rastogi, M.K.J. Siddiqui, M.M.K. Reddy, R.S. Bharti,

Asif M. Khan. Possible mechanism of pesticide toxicity-related oxidative stress leading to airway narrowing. Redox Report, 2006, 11(4), 159-162. (IF: 1.522)

72. M M Krishna Reddy, Sanjukta A. Kumar, M. Sudhershanan, Priyankar Ghosh, R K Sarin and R B

Sashidhar. Quantitative evaluation of twenty-eight mineral elements as possible chemical markers for the source identification of Indian opium. Journal of AOAC International, 2005, 88(5), 1469 - 1484. (IF: 0.918)

Mohana Krishna R Mudiam

73. M M Krishna Reddy, G. Jayshanker, K. M Varshney, R K Sarin and R B Sashidhar. Evaluation of fatty

acids as possible biochemical markers for source identification of Indian opium. LC-GC Europe, 2005, 18, 541-551. (The article was featured on the cover page of the issue). (IF: 0.620)

74. Mudiam Mohanakrishna Reddy, Gurumurthy Jayshanker, Velayudhan Suresh, Rajendra Kumar Sarin and R B Sashidhar, “Principal opium alkaloids as possible biochemical markers for the source identification of Indian opium”. Journal of Separation Science, 2005, 28, 1558 – 1565. (IF: 2.737)

75. M M Krishna Reddy, Priyankar Ghosh, S N Rasool, R K Sarin, R B Sashidhar, “Source identification of Indian opium by chromatographic fingerprinting of amino acids. Journal of Chromatography A, 2005, 1088, 158 – 168. (IF: 3.926)

76. Priyankar Ghosh, M M Krishna Reddy, R B Sashidhar, “Quantitative evaluation of sanguinarine as an index of argemone oil adulteration in edible mustard oil by High performance thin layer chromatography” Food Chemistry, 2005, 91(4), 757 - 764. (IF: 4.052)

77. Priyankar Ghosh, M M Krishna Reddy, V B Ramteke, B Sashidhar Rao, “Analysis and Quantitation of diazepam in cream biscuits by high-performance thin-layer chromatography and its confirmation by mass spectrometry”, Analytica Chimica Acta, 2004, 508, 31-35. (IF: 4.712)

78. Priyankar Ghosh, Mudiam Mohanakrishna Reddy, B Sashidhar Rao, Rajendra Kumar Sarin, “Determination of diazepam in cream biscuits by Liquid Chromatography”, Journal of AOAC International, 2004, 87(3), 569-572. (0.918)

79. Neeli Ramakoteswara Rao, Mudiam Mohanakrishna Reddy, K Maya, Beedu Sashidhar Rao, Priyankar Ghosh, Rajendra Kumar Sarin, “Microscopic classification of Indian opium and its application in source identification”, The Microscope, 2003, 51(4), 179-184. (IF: NA)

80. Mudiam Mohanakrishna Reddy; Velayudhan Suresh; Gurumurthy Jayashanker; Beedu Sashidhar Rao; Rajendra Kumar Sarin, “Application of Capillary Zone Electrophoresis in the separation and determination of principal gum opium alkaloids”, Electrophoresis, 2003, 24, 1437-1441. (IF: 3.028) *Corresponding author;

+Equal contribution

PRESENTATIONS, ABSTRACTS IN CONFERENCES 1. Mohana Krishna Reddy Mudiam. Understanding the pesticide induced toxicity in model organisms

through GC-MS based metabolomics approach. International Conference on Biochemistry, Nutrition and Pharmacy in Human Welfare: Recent trends and future challenges” held at Osmania University, Hyderabad during 3-5, September, 2015. (Plenary Talk)

2. Mohana Krishna Reddy Mudiam. Mass spectrometry based metabolomics – A valuable tool to

understand the toxicity of xenobiotics in different model organisms. ISMAS Summer School on Role of Mass Spectrometry in Proteomics and Metabolomics organized by Indian Society for Mass Spectrometry at IICT/CCMB during 15-20, June, 2015. (Invited Talk)

3. Mohana Krishna Reddy Mudiam. Metabolomics by GC-MS. Invited Talk at Workshop on Metabolomics-Applications and Challenges held at Institute of Bioinformatics, Bangalore organized by IOB, Bangalore and Agilent Technologies, Bangalore on 15

th March 2014. (Invited Talk)

4. Mohana Krishna Reddy Mudiam. New sample preparation methods for the Gas Chromatography-Mass

spectrometric Analysis of Chemical Analytes. Invited Talk at 12th

ISMAS Triennial International Conference on Mass Spectrometry held at Cidade-de-Goa, Dona Paula, Goa 403 004 during March 3-8, 2013. (Invited Talk)

5. Mohana Krishna Reddy Mudiam, Ratnasekhar Ch, Prem Narain Saxena. Gas chromatography-mass spectrometry based metabolomic approach for optimization and evaluation of earthworm sub-lethal responses to carbofuran. Young Scientist Award presentation (above 30 years age category) at International Conference on Environmental Impact on Human Health and Therapeutic Challenges (ICEHT-2012) and 6

th Annual Convention of Association of Biotechnology and Pharmacy (ABAP)

held at Department of Virology, Sri Venkateswara University, Tirupati during 20-22 December, 2012.

Mohana Krishna Reddy Mudiam

6. Mohana Krishna Reddy Mudiam, Abhishek Chauhan, Krishna P Singh, Shailendra K Gupta, R C Murthy. Determination of t,t-muconic acid in urine using molecular imprinted polymer coupled with dispersive liquid-liquid microextraction. Invited Talk at International Symposium on Environmental Impacts, Health Implications and Therapeutic Approaches & A Special Symposium on Water Quality and Aquatic Biotechnology (ISEHT) held at Vikram Simhapuri University, Nellore during July 15-17, 2012. (Invited Talk)

7. Mohana Krishna Reddy Mudiam, Ratnasekhar Ch, C Kesavchandran, Md. Fareed, R.C Murthy, Vipin

Bihari. Can hair amino acids be an indicator for pesticide exposure – A targeted metabolomic approach using gas chromatography-mass spectrometry? Presented at Andhra Pradesh Science Congress-2012 (APSC-2012) held at Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh during 14-16 November, 2012. (Oral Presentation)

8. Mohana Krishna Reddy Mudiam, Rajeev Jain, Amit Kumar Singh, V P Sharma, R C Murthy. Quantitative Evaluation of Bisphenol A in Water and Food samples using Ethyl Chloroformate derivatization followed by SPME/GC-MS Analysis. Poster presented at “National Seminar on Recent Advances in Analytical Sciences – Indian perspective” held at Indian Institute of Chemical Technology, Hyderabad in association with ISAS-Hyderabad Chapter on 20-21

st January 2011.

9. Abhay Bajaj, M K Reddy, Ashutosh Pathak, Saurabh Shukla and Natesan Manickam. Isolation and

characterization of Pseudomonas sp. that degrades alpha-endosulfan and its metabolites. Awarded Best Poster at 3rd International Congress on Bioprocesses in Food Industries, 2008 & 5

th Convention of

the Biotech Research Society of India held at Osmania University during November 6-8, 2008.

10. Mohana Krishna R Mudiam, Zhenhua Lin, Srinivas K Kumar, Richard Roden, Saeed R Khan. Candidate Proteasome Inhibitors that prevent E6 mediated p53 degradation and selectively kill HPV+ Cervical Cancer cells. Poster presented at AACR-NCI-EORTC International Conference on “Molecular Targets and Cancer Therapeutics – Discovery, Biology and Clinical applications” held at San Francisco during October 22-26, 2007.

11. M M Krishna Reddy, Jai Raj Behari. “Organochlorine pesticide residues in drinking water of Lucknow

city”. Poster presented at Indian Science Congress held on 3 – 7 January 2006 at Hyderabad.

12. M M Krishna Reddy, R K Sarin, Y K S Rathore, Priyankar Ghosh, R B Sashidhar. Analytical and chemometric approach in the source identification of Indian opium (Papaver somniferum L). Oral presentation at 16

th Annual Forensic Science Conference held on November 2006 at Raipur.

13. M M Krishna Reddy, Priyankar Ghosh, R B Sashidhar. “Quantitative evaluation of sanguinarine in

argemone oil by Capillary Zone Electrophoresis”. Abstract published in 18th International Symposium on MicroScale Bioseparations (MSB), California Separation Science Society, USA. February 14 – 17

th, 2005.

14. M M Krishna Reddy, Priyankar Ghosh, S N Rasool, R K Sarin, R B Sashidhar. “Source identification of

Indian opium based on chromatographic fingerprinting of amino acids”, 25th International Symposium on

Chromatography held at Paris, France on October 2004 (abstract published).

Mohana Krishna R Mudiam

RESEARCH SUMMARY

1. Senior Scientist at CSIR-Indian Institute of Toxicology Research (2009 – 2013): My research mainly focuses on the understanding the toxicity pattern of xenobiotics in model organisms (Drosophila melanogaster, C elegans, earth worms, rat/mice etc.) through metabolomics approach. We want to identify the specific markers for a specific xenobiotic exposure and to identify the pathway affected due to exposure. Further, I am also engaged in the synthesis and application of molecularly imprinted polymers as a effective sample cleanup tool in analytical method develop and also exploring the possibility of alkyl chloroformates as novel derivatizing agents for the quantitative evaluation of chemical compounds having polar functional groups using GC-MS/MS. Apart from my research, I am extensively supporting the institutional R&D activities for the mass spectral characterization of biotransformed products of Xenobiotics and engaged in NABL and GLP activities of this institute.

2. Visiting Scientist at Sidney Kimmel Comprehensive Cancer Centre at Johns Hopkins, Baltimore, USA (2007-2008): Proteasome inhibitors have potential for the treatment of cervical cancer. Our focus is to develop potent second generation proteasome inhibitors which can prevent E6-mediated degradation of more of its target cellular proteins, based on 1,3-diphenylproten-1-one based derivatives lacking the boronic acid moieties bearing a variety of amino acid substitutions. Through a series of modifications of this family, including attachment of various peptides to target different proteolytic activities of the proteasome, we have explored the chemical space surrounding this compound to develop comprehensive structure-activity relationships. The new lead compound RA-1 inhibits proteasomal activity and has improved dose-dependent antiproliferative and proapoptotic properties in cervical cancer cells containing human papillomavirus. Further, it induces synergistic killing of cervical cancer cell lines when tested in combination with an FDA approved proteasome inhibitor. Exploration of the potential mechanism of proteasomal inhibition by our lead compound using in silico docking studies suggests that the carbonyl group of its oxopiperidine moiety is susceptible to nucleophilic attack by the γ-hydroxythreonine side chain within the catalytic sites of the proteasome.

3. Scientist at CSIR-Indian Institute of Toxicology Research (2005–2009): I was involved in the studies for the evaluation of genotoxicity and induction of stress markers of benzene, toluene and xylene in Drosophila melanogaster and attenuation effect of phytochemicals. I also worked on the biodegradation of kraft lignin (a waste polymer by-product from kraft pulping process in the pulp and paper industry) using bacteria’s like Paenibacillus sp., Aneurinibacillus aneurinilyticus and Bacillus sp. and subsequent characterization of the low molecular weight aromatic compounds by using Gas Chromatography – Mass Spectrometry (GC-MS).I worked for the creation of the analytical facility for the development of novel analytical methods in the accurate and precise determination of chemical pollutants by novel sample cleanup technique, Solid Phase Microextraction (SPME) interfaced with HPLC and GC/MS. I was actively involved in the area of Environmental Biotechnology, especially in the bioremediation of organochlorine pesticides, dechlorination with Sphingomonas sp NM-05 strain and subsequent characterization of biotransformed products by GC-MS.

4. Doctoral work at Central Forensic Science Laboratory, Hyderabad: My doctoral work involved the

application of metabolomics for the evaluation of possible markers to pinpoint the source of Indian opium. Initially I initiated the development of novel analytical methods/procedures for the accurate and precise determination of secondary metabolites (principal opium alkaloids) by Capillary Electrophoresis (CE), amino acids by Liquid Chromatography with post column o-pthalaldehyde derivatization, fatty acids by Gas Chromatography –Mass Spectrometry (GC-MS), trace metals including rare earths by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) in Indian opium (Plant material) and analyzed the opium samples collected from different licit opium growing divisions of India by these methods. The analytical data of opium samples were subjected to chemometric analysis (multivariate analysis) in order to identify true discriminators for source identification of Indian opium. The inferences drawn from the chemometric analysis suggested that amino acids (D, E, G, A, F and K) and all the principal alkaloids were essential for predicting the source of Indian opium. The methodology developed coupled with chemometric analysis was further validated by analyzing blind opium samples of known origin and seized opium samples. The parameters such as fatty acid profile and trace elements including rare earths were found to be inferior markers for source identification. However, the analytical data of fatty acid profile and trace elements including rare earths were of immense value in differentiating Indian opium from opium grown areas in other parts of world. The analytical data provided detailed information that contributed to the National database on Indian opium. Apart from my doctoral work, I was initiated and developed novel analytical methods for the determination of diazepam in cream biscuits and evaluation of sanguinarine as an index of argemone oil adulteration in edible mustard oil by Reversed Phase High Performance Liquid Chromatography (HPLC) and High Performance Thin Layer Chromatography (HPTLC) respectively. These methods resulted in

Mohana Krishna Reddy Mudiam

accurate and precise analysis of analytes with the advantage like reliability, high selectivity and sensitivity, reducing cost and time of analysis.

5. Trainee Chemist at Dr. Reddy’s Research Foundation, Hyderabad: I contributed to a research

program in the design and synthesis of second-generation oxazolidinones with a primary goal of identifying compounds with increased potency against Gram-positive as well as fastidious Gram-negative pathogens compared to Linezolid and in the synthesis and scale up of 3-methoxy-4-methyl benzoic acid, a starting material for the synthesis of the drug, viz., Zafirlukast at National Chemical Laboratory, Pune.

Ph.D. students working/completed

S.No. Name of the student Thesis Title Registration details Status Presently working at

1 Mr. Rajeev Jain (UGC-SRF)

Development of new chemical derivatization assays for quantitative evaluation of chemical analytes with polar functional groups

Jamia Hamdard University, New Delhi

Awarded Junior Scientific Officer (Toxicology) Central Forensic Science Laboratory, Directorate of Forensic Science Services, Ministry of Home Affairs, Lachit Borphukan Path, Guwahati, Assam, India.

2 Mr. Ch Ratnasekhar (CSIR-SRF)

Metabolic investigations of the effects of xenobiotics on human and model organisms

AcSIR Chemical Sciences, CSIR-IITR, Lucknow

Awarded Post-doctoral Research Associate University of Cambridge Metabolic Research Laboratories (Level 4),Wellcome Trust-MRC Institute of Metabolic Science,Box 289 Addenbrooke’s Hospital Hills Road, Cambridge, CB2 0QQ E-mail: rc668@medschl.cam.ac.uk

3 Mr. Abhishek Chauhan (UGC-SRF)

Synthesis and application of molecularly imprinted polymers for the selective determination of biotransformed and/or bioactive substances in complex samples

AcSIR Chemical Sciences, CSIR-IITR, Lucknow

Awarded Management Trainee Employee Id: 62448 R & D, Polymer Division North Site, Atul Limited

4 Mr. Vivek Pandey (BBAU Fellow)

Synthesis, Characterization and Modification of Quantum Dots for Analytical and Biomedical Applications

Department of Applied Chemistry, School of Physical Sciences, Babasaheb Bhimrao Ambedkar University, Lucknow

Awarded Research Associate Analytical Chemistry Laboratory, Regulatory Toxicology Group, CSIR-Indian Institute of Toxicology Research MG Marg, Lucknow – 226 001

5 Mr. Amit K Singh (PA-II, GAP-270)

Understanding the nanoparticles toxicity in different model organims using mass spectrometry based metabolomics

Faculty of Science, Jadavpur University, Kolkata

Ongoing Associate Scientist, GVKBio, Hyderabad

6 Ms. Tejasvi Bhatia (UGC-SRF)

Development of sol-gel based molecularly imprinted polymers for the selective determination of chemical entities of biological interest (ChEBI)

AcSIR Chemical Sciences, CSIR-IITR, Lucknow

Ongoing

7 Mr. Manoj K Gupta (UGC-SRF)

New analytical approaches for the quantitative determination of chemical analytes in complex food and biological matrices

AcSIR Chemical Sciences, CSIR-IITR, Lucknow

Ongoing

8 Mr. Satyajeet Rai, Project Fellow at CSIR-IITR, Lucknow

Studies on pesticide residues in human diet, their hazards and evaluation of residual toxicity

Department of Chemistry, Integral University, Lucknow

Ongoing

9 Mr. Raju D Manager, GVKBIO, Hyderabad

NMR Metabolomics (Topic yet to decide) AcSIR Chemical Sciences, CSIR-IITR, Lucknow

Joined on Jan, 2015

10 Mr. Deviprasad R, Scientist, GVKBIO, Hyderabad

LC-MS/MS (Orbitrap/QqQ) based metabolomics (topic yet to decide)

AcSIR Chemical Sciences at CSIR-IITR, Lucknow

Joined on August, 2015

11 Mr. Hussain Sheik UGC-Junior Research Fellow

Metabolomics AcSIR Chemical Sciences at CSIR-IICT, Hyderabad

Joined on Dec, 2015