Module 2; Insect senses; How to hear with your legs and ...ecoevodevo.com/pdffiles/OutreachE.pdf ·...
Transcript of Module 2; Insect senses; How to hear with your legs and ...ecoevodevo.com/pdffiles/OutreachE.pdf ·...
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Insect senses:how to hear with your legs and taste with your feet
(yum!)
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INSECT SENSES
PART I: The obvious:
Smell
VisionHearing
PART II: The less obvious: A sense of time and direction
PART III: Smell revisited
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INSECT VISION
INSECT VISION
Ocelli•simple lense eyes, •good for contrasts•not image forming•many insects have lost them
Compound eyes (= sum
of 100s-1000s of omm
atidia)
Insects see with their eyes!!!
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APOSITION eye
pigment cells- prevent the same light wave from being seen by multiple ommatidia
ommatidium
- with rhodopsin- absorbs photons and
generates APnerve cells
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SUPERPOSITIONeye
pigment cells!!
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SUPERPOSITIONeye
pigment cells!!
APOSITION eye
pigment cells
CONSEQUENCES???
• high resolution
What about crepuscular insects?
• low sensitivity, i.e. needs much light• only good for day-active insects
• low resolution• high sensitivity• only good for night-active insects
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INSECT SENSES
PART I: The obvious:
Smell
VisionHearing
PART II: The less obvious: A sense of time and direction
PART III: Smell revisited
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Insects see with their eyes, but do they hear with their ears?
What, really,is an insect ear?
To understand how insects hear, ironically we first need to understand how they breathe...(no lungs, no haemoglobin, instead a bunch of inter-connected tubes called trachea!)
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Insects differ in the region of their hearing organ
But regardless of placement it is always part of the tracheal system!!
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Insects differ in the reagion of their hearing organ But regardless of placement it is always part of
the tracheal system!!
So why is it good to have two ears, and not just one?
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INSECT SENSES
PART I: The obvious:
Smell
VisionHearing
PART II: The less obvious: A sense of time and direction
PART III: Smell revisited
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Insects use smell to detect many different things:
Food
Nest
Partners
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PHEROMONES
1a) General Definition
• Chemical substances released by one individual and receivedby another individual of thesame species
• Once received pheromonestrigger the expression of aparticular behavior or developmental program
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PHEROMONES3) Reception and Recognition – sensory apparatus
• insects use chemoreceptors (olfactory sensilli)to receive and recognize pheromones→ consist of:
a) sensory neuron that projects dendrites into cuticularoutgrowth with pores
b) trichogen cell that provides the shaft for the neuronc) a tormogen cell that provides an anchor or socket
1) molecules enter sensillum through pores
2) bind to olfactory binding proteins (OBP) and gettransported through the pore tubules to dendrites
3) OBP-molecule complex recognized by particulardendrite-surface proteins
4) causes a local change in membrane potential
5) if enough of the same molecules affect the dendrites this generates action potential which is then relayed to the brain
Steps in odor recognition
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PHEROMONES
1) molecules enter sensillum through pores
2) bind to olfactory binding proteins (OBP) and gettransported through the pore tubules to dendrites
3) OBP-molecule complex recognized by particulardendrite-surface proteins
4) causes a local change in membrane potential
5) if enough of the same molecules affect the dendrites this generates action potential which is then relayed to the brain
Steps in odor recognition
ensure specificity
What about sensitivity?
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PHEROMONES4b) Sensitivity - continued
Example: Bombyx mori (Saturniidae)
• 2 large, feathery antennae, 17 000 total3000 pores/ sensillum; = 45 Mill. pores/moth
?
• main purpose: detection of female sex pheromone Bombycol
• discovered by Adolph Butenandt 1957
• purified Bombycol using 1 000 000 cocoons
• after many extractions these yielded 6.4 mg Bombycol
• males were able to recognize concentrations as low as 1 x 10-12 microgram / ml.
• tested attractiveness of pure Bombycol using flutterresponse assay
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INSECT SENSES
PART I: The obvious:
PART II: The less obvious:
Smell
VisionHearing
A sense of time and direction
PART III: Smell revisited
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Example: Honey bees
• Conclusion: somehow information isexchanged in the hive that allows nest mates to locate new forage site
• when initial forager is prevented from returning to food source, its nest mates still appear quickly at the new forage site
• individual forager bees identify forage sites, then return to nest andreturn with additional nest mates
Background:• hones bees rely exclusively on pollen and nectar as food resources
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Background:• we now know that bees use the
waggle dance to communicate:
(a)quality of forage site
(a)distance away from nest site
(a)direction relative to current position of the sun
Dance language of honey bees
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(d) angle and direction of straight part relative to gravity indicate angle and direction of forage site relative to the sun when viewed from the hive
(b) intensity of dance (rounds/time) and intensity of buzzing on straight part indicate quality of forage site
Components of the waggle dance:
(c) length of straight part indicatesdistance away from hive
(a) dance consits of figure-8 type dance with; bees buzz their wings and waggle abdomen during straight part
Dance language of honey bees
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Communicating direction of foragesite relative to the sun
Dance language of honey bees
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Communicating direction of foragesite relative to the sun
Dance language of honey bees
This also works on a cloudyday when the sun is not visible! How??
Bees (as well as many other organisms) can see polarizedlight and orient accordingly
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How precise is it?
Dance language of honey bees
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Dance language of honey bees
But then: a simple experiment with an unusual outcome(Lindauer 1960)
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Dance language of honey bees
predictedobserved
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INSECT SENSES
PART I: The obvious:
PART II: The less obvious:
Smell
VisionHearing
A sense of time and direction
PART III: Smell revisited
A simple and highly reliable experiment with termites…
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What you need
• A cheap ball pen, blue
• A piece of paper, clean
• A local termite, alive
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What you should do
• Using the blue pen draw a circle on the paper
• Carefully release the termite in the center of the circle
• Carefully watch the termites behavior
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The idea behind this exercise…• The termite will soon do something that will make
you think
• It does the same to students, school kids, anyone who sees this for the first time; you can’t help it
• If provides a wonderful opportunity to introduce the scientific method, generate hypotheses, and go test them…
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TRAIL-PHEROMONES• widespread in social insects
• use trail pheromones to mark trail to foodsources or nest entrances
• released by specialized glands and dipped onto substrate
• most commonly studied in ants and termites
• also in the most primitive bees (Melliponinae) (sweat bees; have to land and deposit theirpheromone on plants along their flight path).
Honeybees no longer use trail pheromones.How do honey bees tell their colony mates where to go?
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SEX-PHEROMONES
A) Attraction pheromones (long range)Example: Bombycol, Bombyx mori
B) Courtship pheromones (short range)Example: Danaidone, Danaus gilippus
Queen butterfly
• released by males via a pair of glands located at the tip of their abdomen
• resembles structurally a class of chemical compounds found in certain flowering plants and known as pyrrolizidine alcaloids
• males cannot produce Danaidone themselves and instead ingest alcaloids from larval host plant and chemically modify them
• females prefer males with high alkaloid concentrations and females have more offspring survive if mated to a male with high alkaloid concentrations in pheromone!?
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SEX-PHEROMONES – cont.
B) Courtship pheromones (short range)Example: Danaidone, Danaus gilippus
• turns out that males convert only a small portion of the alkaloids they ingest to danaidone
• ~80% of alkaloid load stored in small sacs in their reproductive accessory glands.
• during copulation male transfers toxic alkaloids from glands to the female
• female in turn incorporates alkaloids into egg case
• egg predatory insects avoid eggs that contain high alkaloid concentrations