MINING COMPLEX SARCOMA GENOMES: LESSONS FROM OSTEOSARCOMA

MINING COMPLEX SARCOMA GENOMES:LESSONS FROM OSTEOSARCOMA

TWO WAYS OF THINKING ABOUT SARCOMA

TEICHER 2012DEVELOPMENTAL BIOLOGY VIEW OF SARCOMA

GENOMIC VIEW OF SARCOMA

GENOMIC VIEW OF SARCOMAA

CLEARLY

DEFINED ONCOGENIC

PATHWAY

GENOMIC VIEW OF SARCOMANO

SINGLE

CLEARLY

DEFINED ONCOGENIC

PATHWAY

APPROACHES TO COMPLEXITY LARGE INTEGRATED GENOMICS STUDIES

INTEGRATION WITH GWAS STUDIES

COMPARATIVE ONCOLOGY

COMMONALITIES AMONG COMPLEX SARCOMAS

GOAL: A PATH TO CLINICAL RELEVANCE

GLOBAL INCIDENCEInternational Journal of Cancer Volume 125, Issue 1, pages 229-234, 3 FEB 2009 DOI: 10.1002/ijc.24320 http://onlinelibrary.wiley.com/doi/10.1002/ijc.24320/full#fig1

EPIDEMIOLOGY CONNECTS BONE GROWTH TO TUMOR RISKMirabello et al.Cancer Causes Control. 2011 Jun;22(6):899-908.

MINIMAL SURVIVAL IMPROVEMENT IN RECENT DECADESWhelan Ann. Oncol 2011

BONE DIFFERENTIATION GENESA CONCEPTUAL FRAMEWORK FOR OSTEOSARCOMA

BONE DIFFERENTIATION GENESMORPHOGENETIC,GROWTHANDDIFFERENTIATIONREGULATING SIGNALS

A CONCEPTUAL FRAMEWORK FOR OSTEOSARCOMAENDOCRINE

AUTOCRINE

PARACRINE

DERANGED GROWTH INVASIVE ABERRANT DIFFERENTIATION MASSIVE GENOME INSTABILITY GROSSLY PERTURBED GENE EXPRESSION

MASSIVE GENOME INSTABILITY DERANGED GROWTH INVASIVE ABERRANT DIFFERENTIATIONA SHATTERED GENOME

MASSIVE GENOME INSTABILITY DERANGED GROWTH INVASIVE ABERRANT DIFFERENTIATION

EVIDENCE BASED CATEGORIES OF GENES ALTERED IN OS OR WHICH CONFER PREDISPOSITION TO OS THAT CAN CONTRIBUTE TO THE TUMOR PHENOTYPE:

CELL CYCLE, DNA REPLICATION AND REPAIR

SIGNAL TRANSDUCTION

BONE DIFFERENTIATION

CELL CYCLE, DNA REPLICATION AND REPAIR

GENE EVIDENCE

TP53 GERMLINE MUTATION, TUMOR MUTATION, MOUSE MODELS

RB1 GERMLINE MUTATION, TUMOR MUTATION, MOUSE MODELS

CDKN2A DELETIONCCNE1 AMPLIFICATIONCDK4 AMPLIFICATIONMDM2 AMPLIFICATIONCDC5L AMPLIFICATIONCOPS3 AMPLIFICATIONREQL4 GERMLINE MUTATION

SIGNAL TRANSDUCTION

GENE EVIDENCE

PTEN DELETION

PIK3CA MUTATION

RAS MUTATION

IGF1R AMPLIFICATION

TRANSCRIPTION FACTORS IN BONE DIFFERENTIATIONPPARGCEBPsSOX 5,6,9RUNX2SP7 (OSX)RUNX2OTHER TFSSATB2TWIST DLXMSXAP1EGR2ATF4

BONE DIFFERENTIATION

GENE EVIDENCE

RUNX2 AMPLIFICATION

SATB2 LOSS

RB1 MODELS

TP53 MODELS

UNCERTAIN FUNCTION

GENE EVIDENCE

LSAMP DELETION(REGION?)

TARGET OSTEOSARCOMA PROJECT

TARGET OSTEOSARCOMA PROJECT COMPREHENSIVE GENOMIC ANALYSIS

ARRAYS: mRNA,miRNA, DNA Methylation, SNPS

SEQUENCING: EXOME, TRANSCRIPTOME, WHOLE GENOME ON SELECT SAMPLES

OSNBLTHE TURBULENT GENOME OF OSTEOSARCOMA

WHOLE GENOME SEQUENCINGSOMATIC STRUCTURAL VARIATIONS

WHOLE GENOME SEQUENCINGSTRUCTURAL VARIATIONS: COPY NUMBER LOSS

WHOLE GENOME SEQUENCINGCOPY NUMBER|||||||||||||||||





MAPPING A PATH TO CLINICAL RELEVANCE

PROFILING ADULT PLEOMORPHIC SARCOMAS HIGH DENSITY SNP ARRAYS

TARGETED SEQUENCING OF 1300 GENESMANY COMMONALITIES WITH OSTEOSARCOMA

FEW TARGETABLE POINT MUTATIONS

STRUCTURAL COMPLEXITY

SHARED REGIONS OF CNA

SHARED SNVS (TP53, RB1)

TELOMERE MAINTENANCE MECHANISMS

COMPLEX GENOME OF UPS

RB1 DELETION IN UPS

CHROMOSOME 12 AMPLIFICATION IN A LEIOMYOSARCOMA

TWO MODES OF TELOMERE MAINTENANCE

TELOMERE MAINTENANCE IN SARCOMAHeaphy et al,AJP 179:1609 (2011)Durant, J. Cancer3:67 2012

Heaphy et al,AJP 179:1609 (2011)%

Embryonic Kidney w/AdenovirusPancreatic Cell LineNormal DNAOsteosarcoma

Chart1

430.43569661272.180351320572.1803513205

591.482656339533.656590606333.6565906063

626.907587114741.641917896541.6419178965

2647.625877121883.671345743983.6713457439

2917.5608678189258.339763908258.339763908

3058.3631863245193.2370378882193.2370378882

3930.8974797217142.8629553429142.8629553429

2965.9060629489128.8614791915128.8614791915

1044.0398759024105.3062468698105.3062468698

1743.200544063827.860146534527.8601465345

1105.4939158871236.5044224405236.5044224405

1666.6900469216120.102683104120.102683104

Cell Line Name

Relative Telomere Length = 2^(Ct)

Relative Telomere Repeat Content

Telomere Cts

Telomere

8.17.12 Nano String Samples (Biological Replicates)

No.NameT CtAvg CtStdevCV

1A16.0615.990.070.44%

2A15.98

3A15.91

4B19.319.140.170.89%

5B18.97

6B19.16

7C15.9615.760.181.14%

8C15.59

9C15.73

10D16.7216.70.030.18%

11D16.67

12D16.69

13E17.07170.070.41%

14E16.93

15E17.01

16F18.818.780.030.16%

17F18.74

18F18.8

19NTC28.9328.650.391.36%

20NTC28.38

8.20.12 Nano String Samples (Technical Replicates)


1A17.9117.870.080.45%

2A17.92

3A17.77

4B19.4919.410.110.57%

5B19.47

6B19.28

7C16.516.520.040.24%

8C16.5

9C16.57

10D17.2917.230.060.35%

11D17.24

12D17.17

13E17.8217.710.120.68%

14E17.73

15E17.58

16F19.0219.140.10.52%

17F19.19

18F19.22

19NTC29.3328.581.023.57%

20NTC28.98

21NTC27.42

8.21.12 Cell Lines MMQPCR


1MIA-Paca-2_119.3319.340.090.47%

2MIA-Paca-2_119.44

3MIA-Paca-2_119.27

4Capan-121.4221.440.030.14%

5Capan-121.42

6Capan-121.48

7HT-2917.5817.590.030.17%

8HT-2917.63

9HT-2917.56

10A37518.3118.060.221.22%

11A37517.89

12A37517.97

13SW-141718.818.880.140.74%

14SW-141718.8

15SW-141719.04

16MDA-MB-45319.1119.250.130.68%

17MDA-MB-45319.37

18MDA-MB-45319.28

19UML2118.1218.130.020.11%

20UML2118.11

21UML2118.15

22MCF718.9118.930.020.11%

23MCF718.93

24MCF718.95

25BT-2019.4219.620.271.38%

26BT-2019.93

27BT-2019.52

28NTC30.1329.860.381.27%

29NTC30.03

30NTC29.43



1MIA-Paca-2_119.4319.430.050.26%

2MIA-Paca-2_119.48

3MIA-Paca-2_119.39

4Capan-121.4121.370.040.19%

5Capan-121.33

6Capan-121.39

7HT-2917.6617.680.090.51%

8HT-2917.78

9HT-2917.59

10A37518.1218.150.040.22%

11A37518.12

12A37518.19

13SW-141718.8218.760.060.32%

14SW-141718.7

15SW-141718.76

16MDA-MB-45319.319.370.070.36%

17MDA-MB-45319.45

18MDA-MB-45319.35

19UML2117.9317.990.060.33%

20UML2117.99

21UML2118.06

22MCF719.319.330.060.31%

23MCF719.39

24MCF719.29

25BT-2019.719.660.080.41%

26BT-2019.72

27BT-2019.57

28NTC29.1629.390.331.12%

29NTC29.76

30NTC29.24



1HT-2917.1317.160.020.12%

2HT-2917.16

3HT-2917.18

4A37517.3217.260.060.35%

5A37517.24

6A37517.21

7SW141718.418.370.020.11%

8SW141718.38

9SW141718.35

10MDA-MB-45318.6318.630.020.11%

11MDA-MB-45318.65

12MDA-MB-45318.62

13UML2117.2817.220.080.46%

14UML2117.26

15UML2117.13

16MCF718.6118.760.221.17%

17MCF719.02

18MCF718.65

19BT-2018.918.870.060.32%

20BT-2018.8

21BT-2018.92

22Promega17.2417.180.050.29%

23Promega17.13

24Promega17.16

25NTC28.1227.870.51.79%

26NTC28.19

27NTC27.3



1HT-2917.8917.810.070.39%

2HT-2917.76

3HT-2917.78

4A37517.917.840.070.39%

5A37517.85

6A37517.77

7SW141719.0719.040.060.32%

8SW141719.09

9SW141718.97

10MDA-MB-45319.5119.420.080.41%

11MDA-MB-45319.35

12MDA-MB-45319.42

13UML2119.2919.360.090.46%

14UML2119.46

15UML2119.33

16MCF718.1218.210.180.99%

17MCF718.42

18MCF718.1

19BT-2019.7519.810.070.35%

20BT-2019.88

21BT-2019.81

22Promega18.2118.040.150.83%

23Promega17.93

24Promega17.97

25NTC29.9329.890.190.64%

26NTC30.06

27NTC29.68



1HT-2917.2617.30.040.23%

2HT-2917.31

3HT-2917.34

4A37517.3817.410.020.11%

5A37517.41

6A37517.43

7SW141718.8718.820.070.37%

8SW141718.85

9SW141718.74

10MDA-MB-45319.219.190.040.21%

11MDA-MB-45319.16

12MDA-MB-45319.23

13UML2117.7517.720.050.28%

14UML2117.74

15UML2117.67

16MCF718.5218.490.040.22%

17MCF718.49

18MCF718.45

19BT-2019.0519.010.030.16%

20BT-2019

21BT-2018.99

22Promega17.5517.620.070.40%

23Promega17.68

24Promega17.61

25NTC29.5629.550.220.74%

26NTC29.76

27NTC29.32

9.26.12 Promega MMQPCR

No.NameT CtAvg CtStDev

1PROMEGA17.7617.830.120.67%

2PROMEGA17.85

3PROMEGA17.8

4PROMEGA17.7

5PROMEGA17.76

6PROMEGA17.74

7PROMEGA17.77

8PROMEGA17.73

9PROMEGA17.66

10PROMEGA17.68

11PROMEGA17.74

12PROMEGA17.63

13PROMEGA17.81

14PROMEGA17.71

15PROMEGA17.76

16PROMEGA17.72

17PROMEGA17.69

18PROMEGA17.78

19PROMEGA17.81

20PROMEGA17.81

21PROMEGA17.93

22PROMEGA17.88

23PROMEGA17.96

24PROMEGA18.05

25PROMEGA18

26PROMEGA18.12

27PROMEGA17.89

28PROMEGA18

29PROMEGA17.94

30PROMEGA17.92

31PROMEGA17.89

32PROMEGA17.92

33PROMEGA17.8

34PROMEGA17.9

35PROMEGA17.92

36PROMEGA17.92

9.28.12 DP Promega MMQPCR


1PROMEGA G152A18.1318.060.140.78%

2PROMEGA G152A18.04

3PROMEGA G152A18.07

4PROMEGA G152A17.93

5PROMEGA G152A17.92

6PROMEGA G152A17.95

7PROMEGA G152A17.94

8PROMEGA G152A17.93

9PROMEGA G152A17.9

10PROMEGA G152A17.86






















32PROMEGA G152A18.2


34PROMEGA G152A18.1




No.NameT CtT Ct AvgT StdevCV

17.680.231.30%

2PROMEGA G152A17.53

3PROMEGA G152A17.66

4PROMEGA G152A17.56

5PROMEGA G152A17.28

6PROMEGA G152A17.42

7PROMEGA G152A17.34

8PROMEGA G152A17.31

9PROMEGA G152A17.38




14PROMEGA G152A17.5








22PROMEGA G152A17.8










33PROMEGA G152A17.8




NTC Ct AvgNTC Stdev

29.930.72

1NTC29.55

13NTC29.48

25NTC30.76

SCG Cts

SCG

8.17.12 Nano String Samples (Biological Replicates)

No.NameS CtAvg CtStdevCV

1A26.5526.430.110.42%

2A26.36

3A26.37

4B28.428.270.160.57%

5B28.1

6B28.31

7C27.9527.580.331.20%

8C27.33

9C27.46

10D27.5627.590.090.33%

11D27.52

12D27.69

13E27.1127.080.070.26%

14E27.01

15E27.13

16F27.9728.040.060.21%

17F28.07

18F28.09

19NTCunknown

20NTCunknown



1A28.7428.760.030.10%

2A28.74

3A28.79

4B29.0229.070.180.62%

5B28.92

6B29.26

7C28.4628.430.080.28%

8C28.34

9C28.49

10D28.4228.410.030.11%

11D28.37

12D28.43

13E28.0928.180.160.57%

14E28.08

15E28.36

16F28.728.710.040.14%

17F28.68

18F28.76

19NTC

20NTC

21NTC31.58?



1MIA-Paca-2_129.1429.060.070.24%

2MIA-Paca-2_129

3MIA-Paca-2_129.04

4Capan-130.0830.090.030.10%

5Capan-130.08

6Capan-130.12

7HT-2928.8828.840.040.14%

8HT-2928.79

9HT-2928.85

10A37529.2328.950.250.86%

11A37528.77

12A37528.84

13SW-141729.3529.510.170.58%

14SW-141729.5

15SW-141729.68

16MDA-MB-45329.9730.030.060.20%

17MDA-MB-45330.07

18MDA-MB-45330.06

19UML2129.0129.020.010.03%

20UML2129.02

21UML2129.03

22MCF728.8628.890.090.31%

23MCF728.81

24MCF728.99

25BT-2029.6629.670.190.64%

26BT-2029.86

27BT-2029.48

28NTC

29NTC

30NTC



1MIA-Paca-2_129.1929.190.040.14%

2MIA-Paca-2_129.16

3MIA-Paca-2_129.23

4Capan-130.2630.250.180.60%

5Capan-130.06

6Capan-130.43

7HT-2929.229.250.080.27%

8HT-2929.22

9HT-2929.34

10A37529.229.220.140.48%

11A37529.09

12A37529.36

13SW-141729.3329.450.110.37%

14SW-141729.56

15SW-141729.45

16MDA-MB-45330.0830.030.070.23%

17MDA-MB-45329.94

18MDA-MB-45330.05

19UML2129.1729.130.040.14%

20UML2129.13

21UML2129.08

22MCF729.5329.590.070.24%

23MCF729.56

24MCF729.67

25BT-2029.8529.920.060.20%

26BT-2029.96

27BT-2029.94

28NTC

29NTC

30NTC


No.NameS CtAvg CtStDevCV

1HT-2928.7828.740.090.31%

2HT-2928.8

3HT-2928.64

4A37528.4628.60.140.49%

5A37528.74

6A37528.61

7SW141729.4429.490.050.17%

8SW141729.51

9SW141729.53

10MDA-MB-45329.8829.910.080.27%

11MDA-MB-45329.85

12MDA-MB-45329.99

13UML2128.95290.10.34%

14UML2128.92

15UML2129.11

16MCF729.2329.30.160.55%

17MCF729.48

18MCF729.18

19BT-2029.4829.450.070.24%

20BT-2029.5

21BT-2029.37

22Promega28.5828.660.060.21%

23Promega28.71

24Promega28.68

25NTC

26NTC

27NTC30.98



1HT-2928.3128.250.050.18%

2HT-2928.23

3HT-2928.21

4A37528.2228.260.050.18%

5A37528.24

6A37528.32

7SW141729.0328.910.140.48%

8SW141728.76

9SW141728.94

10MDA-MB-45329.5129.490.020.07%

11MDA-MB-45329.5

12MDA-MB-45329.47

13UML2128.89290.150.52%

14UML2129.17

15UML2128.95

16MCF728.4528.50.160.56%

17MCF728.68

18MCF728.36

19BT-2028.8329.10.260.89%

20BT-2029.34

21BT-2029.12

22Promega28.4728.360.10.35%

23Promega28.27

24Promega28.33

25NTC

26NTC

27NTC



1HT-2927.8527.780.10.36%

2HT-2927.66

3HT-2927.83

4A37527.6627.640.060.22%

5A37527.56

6A37527.68

7SW141728.8228.820.080.28%

8SW141728.9

9SW141728.73

10MDA-MB-45329.1329.190.090.31%

11MDA-MB-45329.15

12MDA-MB-45329.29

13UML2128.128.040.050.18%

14UML2128.01

15UML2128.02

16MCF728.0928.030.10.36%

17MCF727.91

18MCF728.07

19BT-2028.4828.420.050.18%

20BT-2028.39

21BT-2028.4

22Promega28.1728.10.090.32%

23Promega28.14

24Promega28

25NTC

26NTC

27NTC



1PROMEGA28.2428.230.080.28%

2PROMEGA28.17

3PROMEGA28.26

4PROMEGA28.42

5PROMEGA28.32

6PROMEGA28.25

7PROMEGA28.25

8PROMEGA28.32

9PROMEGA28.24

10PROMEGA28.19

11PROMEGA28.31

12PROMEGA28.25

13PROMEGA28.29

14PROMEGA28.27

15PROMEGA28.22

16PROMEGA28.26

17PROMEGA28.2

18PROMEGA28.23

19PROMEGA28.18

20PROMEGA28.19

21PROMEGA28.27

22PROMEGA28.17

23PROMEGA28.16

24PROMEGA28.16

25PROMEGA28.27

26PROMEGA28.3

27PROMEGA28.09

28PROMEGA28.13

29PROMEGA28.22

30PROMEGA28.18

31PROMEGA28.1

32PROMEGA28.26

33PROMEGA28.06

34PROMEGA28.21

35PROMEGA28.3

36PROMEGA28.38



1PROMEGA G152A28.7128.510.090.32%

2PROMEGA G152A28.45

3PROMEGA G152A28.52

4PROMEGA G152A28.62

5PROMEGA G152A28.47

6PROMEGA G152A28.45

7PROMEGA G152A28.45

8PROMEGA G152A28.37

9PROMEGA G152A28.46




















29PROMEGA G152A28.6









No.NameS CtAvg S CtS StdevCV

28.320.20.71%

2PROMEGA G152A28.25

3PROMEGA G152A28.21

4PROMEGA G152A27.67

5PROMEGA G152A28.25

6PROMEGA G152A28.13

7PROMEGA G152A28.12

8PROMEGA G152A28.07

9PROMEGA G152A28.06


11PROMEGA G152A28.1




















33PROMEGA G152A28.4




1NTC

13NTC

25NTC

TS Ratios

SCG

8.17.12 Nano String Samples (Biological Replicates)Gabi's 7.10.12

No.NameT CtS CtT/SAverage T/SStdevT/S RatiosNameT CtS CtT/SAvg T/SStdevT/S

1A16.0626.551438.15155275571393.0954.46A1393.1A18.3729.352019.80457880241882.8176932866122.72864789591882.8176932866

2A15.9826.361332.5740599725A18.2929.141845.760947427

3A15.9126.371408.5548217751A18.2629.061782.8875536305

4B19.328.4548.7480128186559.049.73B559.0B20.130.131045.5164167242746.1538273767259.5520513891746.1538273767

5B18.9728.1560.2783750455B19.8129.06608.8740428814

6B19.1628.31568.0995696987B20.0629.25584.0710225246

7C15.9627.954067.70686131013628.37380.66C3628.4C17.629.473743.05362013963736.9446557962168.19829108063736.9446557962

8C15.5927.333420.5201179786C17.4929.293565.7751072609

9C15.7327.463396.892859656C17.5229.453902.005239988

10D16.7227.561833.01134526051908.92120.61D1908.9D18.3629.492241.1135001822088.9880877125133.39636082612088.9880877125

11D16.6727.521845.760947427D18.1529.142033.853430655

12D16.6927.692048D18.5829.541991.9973323004

13E17.0727.111052.78855849581082.6630.01E1082.7E19.0629.141082.38647353481036.50254098143.44447124791036.502540981

14E16.9327.011082.3864735348E19.0429995.9986661502

15E17.0127.131112.8164992267E19.3429.351031.1224832581

16F18.827.97576.0299441607615.2035.05F615.2F20.629.99670.9214227755606.609958712559.8424415321606.6099587125

17F18.7428.07643.5908477552F20.9130.13596.3435962718

18F18.828.09625.9918221783F20.6529.76552.5648570903

19NTC28.93NTC30.96

20NTC28.38NTC29.21

NTC29.59


No.NameT CtS CtT/SAvg T/SStdevT/S Ratios

1A17.9128.741820.34981102911901.57151.6999315231A1901.6

2A17.9228.741807.7757364033

3A17.7728.792076.58901461

4B19.4929.02739.2917481075816.20168.9300894395B816.2

5B19.4728.92699.4126114583

6B19.2829.261009.9022894012

7C16.528.463983.99466460073841.69161.5899939716C3841.7

8C16.528.343666.022690521

9C16.5728.493875.052120988

10D17.2928.422241.1135001822311.55122.0073256247D2311.6

11D17.2428.372241.113500182

12D17.1728.432452.4363870596

13E17.8228.091234.74721555531432.75284.1628063751E1432.7

14E17.7328.081305.1500823749

15E17.5828.361758.3420138245

16F19.0228.7820.2955546518761.2752.6679309101F761.3

17F19.1928.68719.0757763779

18F19.2228.76744.433928868

19NTC29.33

20NTC28.98

21NTC27.4231.58?



1MIA-Paca-2_119.3329.14897.6442586276841.856075683776.3631105817MIA-Paca-2_1841.9

2MIA-Paca-2_119.4429754.825839253

3MIA-Paca-2_119.2729.04873.0981291704

4Capan-121.4230.08404.501151674402.64485604483.2151983438Capan-1402.6

5Capan-121.4230.08404.501151674

6Capan-121.4830.12398.9322647862

7HT-2917.5828.882521.38375853042437.8517020052129.8909011409HT-292437.9

8HT-2917.6328.792288.2040587719

9HT-2917.5628.852503.9672887134

10A37518.3129.231937.5260604941897.865726347934.9581234429A3751897.9

11A37517.8928.771884.5443084797

12A37517.9728.841871.5268100698

13SW-141718.829.351499.22375264831586.148573176982.5525778639SW-14171586.1

14SW-141718.829.51663.4929077376

15SW-141719.0429.681595.7290591447

16MDA-MB-45319.1129.971858.59923008951760.144717217297.5656525631MDA-MB-4531760.1

17MDA-MB-45319.3730.071663.4929077376

18MDA-MB-45319.2830.061758.3420138245

19UML2118.1229.011897.65235075151902.113083207220.1724957082UML211902.1

20UML2118.1129.021924.1425903903

21UML2118.1529.031884.5443084798

22MCF718.9128.86989.118800819994.726504518255.4711966519MCF7994.7

23MCF718.9328.81942.2721542399

24MCF718.9528.991052.7885584958

25BT-2019.4229.661209.33648530381060.2788204837129.4939225738BT-201060.3

26BT-2019.9329.86975.501309997

27BT-2019.5229.48995.9986661502

28NTC30.13

29NTC30.03

30NTC29.43



1MIA-Paca-2_119.4329.19867.0671998592867.9648.1112186965MIA-Paca-2_1868.0

2MIA-Paca-2_119.4829.16820.2955546518

3MIA-Paca-2_119.3929.23916.5056726302

4Capan-121.4130.26461.4402368567470.8251.5349199464Capan-1470.8

5Capan-121.3330.06424.611607457

6Capan-121.3930.43526.3942792479

7HT-2917.6629.22977.7357154723066.79341.8076844142HT-293066.8

8HT-2917.7829.222778.3256626128

9HT-2917.5929.343444.3117168792

10A37518.1229.22164.77294706972158.25149.2405046332A3752158.2

11A37518.1229.092005.852769458

12A37518.1929.362304.1197766427

13SW-141718.8229.331458.22740029141656.28200.2201308512SW-14171656.3

14SW-141718.729.561858.5992300895

15SW-141718.7629.451652.0023228869

16MDA-MB-45319.330.081758.34201382451620.00164.4673273278MDA-MB-4531620.0

17MDA-MB-45319.4529.941438.1515527557

18MDA-MB-45319.3530.051663.4929077376

19UML2117.9329.172418.67297060772250.65171.1221318575UML212250.7

20UML2117.9929.132256.7016773153

21UML2118.0629.082076.58901461

22MCF719.329.531200.98298801341228.5493.3586686475MCF71228.5

23MCF719.3929.561152.0598883213

24MCF719.2929.671332.5740599725

25BT-2019.729.851136.19913939751222.9794.3267568561BT-201223.0

26BT-2019.7229.961209.3364853038

27BT-2019.5729.941323.3692985728

28NTC29.16

29NTC29.76

30NTC29.24


No.NameT CtS CtT/SAvg T/SStDevT/S Ratios

1HT-2917.1328.783213.65646498513074.07222.814918982HT-293074.1

2HT-2917.1628.83191.4581182894

3HT-2917.1828.642817.1096435502

4A37517.3228.462256.70167731532618.45327.9537017401A3752618.5

5A37517.2428.742896.3093757401

6A37517.2128.612702.3522012629

7SW141718.429.442105.57711699162222.28108.5174091108SW14172222.3

8SW141718.3829.512241.113500182

9SW141718.3529.532320.1461970858

10MDA-MB-45318.6329.882435.49617152562478.26151.6917131285MDA-MB-4532478.3

11MDA-MB-45318.6529.852352.5342310339

12MDA-MB-45318.6229.992646.7385971456

13UML2117.2828.953258.51743746013511.38457.5994911433UML213511.4

14UML2117.2628.923236.0092133924

15UML2117.1329.114039.6091576048

16MCF718.6129.231573.76018563891486.9782.921076636MCF71487.0

17MCF719.0229.481408.5548217751

18MCF718.6529.181478.583496215

19BT-2018.929.481530.72563060221531.01132.334079016BT-201531.0

20BT-2018.829.51663.4929077376

21BT-2018.9229.371398.8252229165

22Promega17.2428.582592.26950445112863.49243.0173158335Promega2863.5

23Promega17.1328.713061.4512612044

24Promega17.1628.682936.7403479364

25NTC28.12

26NTC28.19

27NTC27.330.98?


No.NameT CtS CtT/SAvg T/SStDevAvg T/S

1HT-2917.8928.311370.0378161011389.319031385725.590844245HT-291389.3

2HT-2917.7628.231418.3520953456

3HT-2917.7828.211379.5671827105

4A37517.928.221278.29048207521373.1190267582113.7388550352A3751373.1

5A37517.8528.241341.842845551

6A37517.7728.321499.2237526483

7SW141719.0729.03995.9986661502937.8514700814106.7696808473SW1417937.9

8SW141719.0928.76814.629359365

9SW141718.9728.941002.926384729

10MDA-MB-45319.5129.5110241073.43680713757.2742432097MDA-MB-4531073.4

11MDA-MB-45319.3529.51136.1991393975

12MDA-MB-45319.4229.471060.1112820136

13UML2119.2928.89776.0468820533800.152894278732.8210511444UML21800.2

14UML2119.4629.17837.5317079632

15UML2119.3328.95786.8800928195

16MCF718.1228.451287.18169551041246.539360856635.1972942792MCF71246.5

17MCF718.4228.681226.2181935298

18MCF718.128.361226.2181935298

19BT-2019.7528.83541.1932367674626.733663341281.8356408829BT-20626.7

20BT-2019.8829.34704.2774108875

21BT-2019.8129.12634.7303423688

22Promega18.2128.471226.21819352981278.860354953246.478385845Promega1278.9

23Promega17.9328.271296.1347522256

24Promega17.9728.331314.2281191044

25NTC29.93

26NTC30.06

27NTC29.68


No.NameT CtS CtT/SAvg T/SStdevAvg T/S

1HT-2917.2627.851541.371428.22118.42HT-291428.2

2HT-2917.3127.661305.15

3HT-2917.3427.831438.15

4A37517.3827.661243.341199.0955.95A3751199.1

5A37517.4127.561136.20

6A37517.4327.681217.75

7SW141718.8728.82989.121022.0535.77SW14171022.1

8SW141718.8528.91060.11

9SW141718.7428.731016.93

10MDA-MB-45319.229.13975.501019.9746.07MDA-MB-4531020.0

11MDA-MB-45319.1629.151016.93

12MDA-MB-45319.2329.291067.48

13UML2117.7528.11305.151281.6840.65UML211281.7

14UML2117.7428.011234.75

15UML2117.6728.021305.15

16MCF718.5228.09760.08743.9952.80MCF7744.0

17MCF718.4927.91685.02

18MCF718.4528.07786.88

19BT-2019.0528.48689.78680.339.43BT-20680.3

20BT-201928.39670.92

21BT-2018.9928.4680.29

22Promega17.5528.171573.761441.39119.39Promega1441.4

23Promega17.6828.141408.55

24Promega17.61281341.84

25NTC29.56

26NTC29.76

27NTC29.32


No.NameT CtS CtT/ST/S AvgStDev

1PROMEGA17.7628.241428.21751434281357.7362240198143.0348426716

2PROMEGA17.8528.171278.2904820752

3PROMEGA17.828.261408.5548217751

4PROMEGA17.728.421686.714403364

5PROMEGA17.7628.321509.6516785061

6PROMEGA17.7428.251458.2274002914

7PROMEGA17.7728.251428.2175143428

8PROMEGA17.7328.321541.3726693489

9PROMEGA17.6628.241530.7256306022

10PROMEGA17.6828.191458.2274002914

11PROMEGA17.7428.311520.1521363242

12PROMEGA17.6328.251573.7601856389

13PROMEGA17.8128.291428.2175143428

14PROMEGA17.7128.271509.6516785061

15PROMEGA17.7628.221408.5548217751

16PROMEGA17.7228.261488.867857736

17PROMEGA17.6928.21458.2274002914

18PROMEGA17.7828.231398.8252229165

19PROMEGA17.8128.181323.3692985728

20PROMEGA17.8128.191332.5740599725

21PROMEGA17.9328.271296.1347522256

22PROMEGA17.8828.171251.9836443567

23PROMEGA17.9628.161176.267115517

24PROMEGA18.0528.161105.1297141805

25PROMEGA1828.271234.7472155553

26PROMEGA18.1228.31160.0730985429

27PROMEGA17.8928.091176.267115517

28PROMEGA1828.131120.556750091

29PROMEGA17.9428.221243.3355616205

30PROMEGA17.9228.181226.2181935298

31PROMEGA17.8928.11184.448700319

32PROMEGA17.9228.261296.1347522256

33PROMEGA17.828.061226.2181935298

34PROMEGA17.928.211269.4606847375

35PROMEGA17.9228.31332.5740599725

36PROMEGA17.9228.381408.5548217751


No.NameT CtS CtT/ST/S AverageStdev

1PROMEGA G152A18.1328.711530.72563060221400.349928647195.3422010069

2PROMEGA G152A18.0428.451360.5742736348

3PROMEGA G152A18.0728.521398.8252229165

4PROMEGA G152A17.9328.621652.0023228869

5PROMEGA G152A17.9228.471499.2237526483

6PROMEGA G152A17.9528.451448.15468787

7PROMEGA G152A17.9428.451458.2274002914

8PROMEGA G152A17.9328.371389.1628313064

9PROMEGA G152A17.928.461509.6516785061

10PROMEGA G152A17.8628.431520.1521363242

11PROMEGA G152A17.9528.541541.3726693489

12PROMEGA G152A17.9128.461499.2237526483

13PROMEGA G152A17.9228.451478.583496215

14PROMEGA G152A17.9628.461448.15468787

15PROMEGA G152A17.8628.381468.3701739682

16PROMEGA G152A17.9428.411418.3520953456

17PROMEGA G152A17.8828.391458.2274002914

18PROMEGA G152A17.9428.391398.8252229165

19PROMEGA G152A17.9828.371341.842845551

20PROMEGA G152A18.0128.421360.5742736348

21PROMEGA G152A18.2628.641332.5740599725

22PROMEGA G152A18.1528.451260.6918792652

23PROMEGA G152A18.2628.571269.4606847375

24PROMEGA G152A18.2728.621305.1500823749

25PROMEGA G152A18.2228.581314.2281191044

26PROMEGA G152A18.2828.491184.448700319

27PROMEGA G152A18.1828.521296.1347522256

28PROMEGA G152A18.2728.681360.5742736348

29PROMEGA G152A18.2228.61332.5740599725

30PROMEGA G152A18.1828.571341.842845551

31PROMEGA G152A18.2428.631341.842845551

32PROMEGA G152A18.228.581332.5740599725

33PROMEGA G152A18.2328.581305.1500823749

34PROMEGA G152A18.128.561408.5548217751

35PROMEGA G152A18.0828.561428.2175143428

36PROMEGA G152A18.1628.631418.3520953456


No.NameT CtS CtT/ST/S AverageStdev

1603.153617744148.099844065

2PROMEGA G152A17.5328.251686.714403364

3PROMEGA G152A17.6628.211499.2237526483

4PROMEGA G152A17.5627.671105.1297141805

5PROMEGA G152A17.2828.252005.852769458

6PROMEGA G152A17.4228.131675.0634159263

7PROMEGA G152A17.3428.121758.3420138245

8PROMEGA G152A17.3128.071734.1343997185

9PROMEGA G152A17.3828.061640.5911093036

10PROMEGA G152A17.4728.171663.4929077376

11PROMEGA G152A17.3828.11686.714403364

12PROMEGA G152A17.3728.131734.1343997184

14PROMEGA G152A17.528.291770.5722497185

15PROMEGA G152A17.5728.321722.1558584396

16PROMEGA G152A17.6328.281606.8282324926

17PROMEGA G152A17.6828.351629.2587187301

18PROMEGA G152A17.6428.361686.714403364

19PROMEGA G152A17.7628.411606.8282324925

20PROMEGA G152A17.6528.361675.0634159263

21PROMEGA G152A17.8328.431552.0937641066

22PROMEGA G152A17.828.421573.7601856389

23PROMEGA G152A17.9328.411428.2175143428

24PROMEGA G152A18.0828.691562.8894299756

26PROMEGA G152A17.9828.531499.2237526483

27PROMEGA G152A17.9528.581584.7065533874

28PROMEGA G152A17.9628.581573.7601856389

29PROMEGA G152A18.0228.551478.583496215

30PROMEGA G152A17.8428.531652.0023228869

31PROMEGA G152A17.9428.341351.1761006314

32PROMEGA G152A17.8928.511573.7601856389

33PROMEGA G152A17.828.41552.0937641066

34PROMEGA G152A17.7828.371541.3726693489

35PROMEGA G152A17.7128.321562.8894299756

36PROMEGA G152A17.7828.361530.7256306022

1NTC29.55

13NTC29.48

25NTC30.76

TS Ratios

54.460459098354.4604590983122.7286478959122.7286478959

9.73484371199.7348437119259.5520513891259.5520513891

380.6574034691380.6574034691168.1982910806168.1982910806

120.6118487876120.6118487876133.3963608261133.3963608261

30.014931580430.014931580443.444471247943.4444712479

35.048515543535.048515543559.842441532159.8424415321

Allison 8.17.12

Gabi 7.10.12

T/S Ratio

Gabi's Summer

151.6999315231151.6999315231122.7286478959122.7286478959

168.9300894395168.9300894395259.5520513891259.5520513891

161.5899939716161.5899939716168.1982910806168.1982910806

122.0073256247122.0073256247133.3963608261133.3963608261

284.1628063751284.162806375143.444471247943.4444712479

52.667930910152.667930910159.842441532159.8424415321

Allison 8.20.12

Gabi 7.10.12

T/S Ratios

Cell Line Tracking

76.363110581776.3631105817

3.21519834383.2151983438

129.8909011409129.8909011409

34.958123442934.9581234429

82.552577863982.5525778639

97.565652563197.5656525631

20.172495708220.1724957082

55.471196651955.4711966519

129.4939225738129.4939225738

T/S Ratios

Summary

48.111218696548.1112186965

51.534919946451.5349199464

341.8076844142341.8076844142

149.2405046332149.2405046332

200.2201308512200.2201308512

164.4673273278164.4673273278

171.1221318575171.1221318575

93.358668647593.3586686475

94.326756856194.3267568561

T/S Ratios

Nano String Summary

8.21.12

8.22.12

T/S Ratios

DNA Concentrations

222.814918982222.814918982

327.9537017401327.9537017401

108.5174091108108.5174091108

151.6917131285151.6917131285

457.5994911433457.5994911433

82.92107663682.921076636

132.334079016132.334079016

243.0173158335243.0173158335

Cell Line

T/S Ratios

222.814918982222.814918982341.8076844142341.8076844142129.8909011409129.8909011409

327.9537017401327.9537017401149.2405046332149.240504633234.958123442934.9581234429

108.5174091108108.5174091108200.2201308512200.220130851282.552577863982.5525778639

151.6917131285151.6917131285164.4673273278164.467327327897.565652563197.5656525631

457.5994911433457.5994911433171.1221318575171.122131857520.172495708220.1724957082

82.92107663682.92107663693.358668647593.358668647555.471196651955.4711966519

132.334079016132.33407901694.326756856194.3267568561129.4939225738129.4939225738

243.0173158335243.0173158335

9.21.12

8.22.12

8.21.12

Cell Line

T/S Ratios

25.59084424525.590844245

113.7388550352113.7388550352

106.7696808473106.7696808473

57.274243209757.2742432097

32.821051144432.8210511444

35.197294279235.1972942792

81.835640882981.8356408829

46.47838584546.478385845

Cell Line

T/S Ratios

341.8076844142341.8076844142129.8909011409129.890901140925.59084424525.590844245222.814918982222.814918982

149.2405046332149.240504633234.958123442934.9581234429113.7388550352113.7388550352327.9537017401327.9537017401

200.2201308512200.220130851282.552577863982.5525778639106.7696808473106.7696808473108.5174091108108.5174091108

164.4673273278164.467327327897.565652563197.565652563157.274243209757.2742432097151.6917131285151.6917131285

171.1221318575171.122131857520.172495708220.172495708232.821051144432.8210511444457.5994911433457.5994911433

93.358668647593.358668647555.471196651955.471196651935.197294279235.197294279282.92107663682.921076636

94.326756856194.3267568561129.4939225738129.493922573881.835640882981.8356408829132.334079016132.334079016

46.47838584546.478385845243.0173158335243.0173158335

8.22.12

8.21.12

9.24.12

9.21.12

Cell Line

T/S Ratios

118.4237452477118.423745247725.59084424525.590844245

55.95111339555.951113395113.7388550352113.7388550352

35.77270676435.772706764106.7696808473106.7696808473

46.067317086346.067317086357.274243209757.2742432097

40.6471141140.6471141132.821051144432.8210511444

52.801095292352.801095292335.197294279235.1972942792

9.4311598079.43115980781.835640882981.8356408829

119.3935737066119.393573706646.47838584546.478385845

9.25.12

9.24.12

Cell Line

T/S Ratios

118.4237452477118.4237452477129.8909011409129.8909011409341.8076844142341.8076844142

55.95111339555.95111339534.958123442934.9581234429149.2405046332149.2405046332

35.77270676435.77270676482.552577863982.5525778639200.2201308512200.2201308512

46.067317086346.067317086397.565652563197.5656525631164.4673273278164.4673273278

40.6471141140.6471141120.172495708220.1724957082171.1221318575171.1221318575

52.801095292352.801095292355.471196651955.471196651993.358668647593.3586686475

9.4311598079.431159807129.4939225738129.493922573894.326756856194.3267568561

119.3935737066119.3935737066

9.25.12

8.21.12

8.22.12

Cell Line

T/S Ratios

Promega Sample

T/S Ratios

9.26.12

10.2.12

Promega Sample

T/S Ratios

9.26.12

10.2.12

Promega Sample

T/S Ratios

Osteosarcoma 8.8.12

NameT CtS CtT/SAverage T/SStdevCVNormalized T/S

Saos-217.4929.474039.6091576048Saos-23930.90142.86295534293.63%2753.89

Saos-217.6129.493769.0886169595

Saos-217.429.363983.9946646007

CAL7217.0230.6412590.0814851114CAL7211088.851339.47533936812.08%9911.84

CAL7216.9830.3610660.5924797803

CAL7216.9930.2810015.8691548534

G29218.0529.62998.4475052966G2922917.56258.3397639088.85%1740.55

G29218.4329.792628.4562382088

G29218.0129.623125.7788599512

HOS19.6529.04670.9214227755HOS626.9141.64191789656.64%-550.10

HOS19.829588.1335577585

HOS19.7529.03621.6677808103

Hu03-N117.6629.343281.1822186071Hu03-N13058.36193.23703788826.32%1881.36

Hu03-N117.929.422936.7403479364

Hu03-N117.7929.322957.1669924301

NY17.4628.782556.5809641505NY2647.6383.67134574393.16%1470.62

NY16.8928.32721.1485472696

NY17.4928.872665.1481199451

OSACL20.7430.04630.3459396326OSACL591.4833.65659060635.69%-585.52

OSACL20.5129.67572.051014693

OSACL20.8730.03572.051014693

U2OS16.3630.4217079.759025621U2OS14033.672673.357539101319.05%12856.66

U2OS16.530.1612944.0368535696

U2OS16.8230.3812077.2134280487

143B20.6329.29404.501151674143B430.4472.180351320516.77%-746.57

143B20.4829.48512

143B20.5929.14374.8059381621

HUO918.4630.033040.3042726483HUO92965.91128.86147919154.34%1788.90

HUO918.6230.082817.1096435502

HUO918.3529.923040.3042726483

promega18.5928.861234.7472155553promega1177.0151.4099746994.37%0.00

promega18.4728.621136.1991393975

promega18.4228.61160.0730985429

NTC27.63

NTC28.57

NTC28.65

cell line test1_7.17.12

NameCtCtT/S RatioAverage T/SStDevCV

MCF10A_520.729.29385.3431673372MCF10A_5368.50825660924.70734227390.0670469164

MCF10A_520.5929340.1435684087

MCF10A_520.5929.16380.038034081

PANC-1_319.5929.13744.433928868PANC-1_3729.195683060313.4315680760.0184197032

PANC-1_319.6629.15719.0757763779

PANC-1_319.7629.26724.077343935

MIA PaCa-2_319.6828.87584.0710225246MIA PaCa-2_3574.923145791119.46909996260.0338638305

MIA PaCa-2_319.7528.86552.5648570903

MIA PaCa-2_319.7928.99588.1335577585

Hs766T_321.0829.02245.5716145473Hs766T_3240.08988241749.49463856250.0395461836

Hs766T_321.1929.03229.1264181576

Hs766T_321.0328.97245.5716145473

293T_318.7928.971160.0730985429293T_31152.07833875017.98555056450.0069314302

293T_318.8128.971144.102029386

293T_318.7428.911152.0598883213

LNCAP_320.0528.46340.1435684087LNCAP_3352.245278394410.55369572340.0299612127

LNCAP_320.0128.5359.5378881889

LNCAP_320.0728.55357.0543785857

NTC29.2

NTC29.34

NTC29.34



PANC-1_420.0329.17564.1754193288PANC-1_4553.087306257870.48750665720.1274437252

PANC-1_420.0828.98477.7128916668

PANC-1_419.9929.26617.3736077777

MCF10A_620.2128.44300.2457470034MCF10A_6288.144906458510.66048541490.0369969594

MCF10A_620.328.45284.0497848494

MCF10A_620.2728.4280.1391875227

MIAPaCa-2_419.7128.41415.8732269344MIAPaCa-2_4407.356055191134.25515854140.0840914431

MIAPaCa-2_419.7828.31369.6458740538

MIAPaCa-2_419.728.47436.5490645852

Hs766T_220.8728.44190.0190170405Hs766T_2177.512233027910.89604692730.0613819495

Hs766T_221.0428.47172.4458978388

Hs766T_221.0728.48170.0717842044

293T_218.1728.261089.9150668322293T_2941.697011141131.83255937560.1399946669

293T_218.4228.13837.5317079632

293T_218.428.21897.6442586276

LNCAP_219.9127.99270.5966183837LNCAP_2250.605723282418.55284716740.074032017

LNCAP_219.9227.87247.2797002048

LNCAP_220.0927.96233.9408512587

NTC29.38

NTC29.68

NTC30.21



PANC-1_120.2529.45588.1335577585PANC-1_1564.500737951623.47185139350.0415798418

PANC-1_120.2729.35541.1932367674

PANC-1_120.1329.27564.1754193288

MCF10A_520.5528.86317.3651711844MCF10A_5314.577660188911.11510063990.0353334074

MCF10A_520.628.94324.0336880564

MCF10A_520.6128.85302.334121326

MIA PaCa-2_120.0928.79415.8732269344MIA PaCa-2_1436.282604220661.07723784290.1399946669

MIA PaCa-2_120.0828.68388.0234410267

MIA PaCa-2_120.9129.89504.9511447006

Hs766T_121.1828.62173.6453539133Hs766T_1201.408563311524.2281505120.1202935472

Hs766T_12128.77218.2745322926

Hs766T_121.0628.79212.3058037285

293T_118.5528.551024293T_11038.344277816112.42250898770.011963767

293T_118.5128.541045.5164167242

293T_118.4828.511045.5164167242

LNCAP_120.0428.11268.7274710075LNCAP_1285.789909684819.1833693450.0671240261

LNCAP_119.9328.19306.5545483824

LNCAP_119.9728.11282.0877096644

NTC28.9

NTC29.46

NTC29.2

7.24.12


CAPAN21.929.72225.9719670504CAPAN233.647866879513.29504849770.0569020752

CAPAN21.8829.7225.9719670504

CAPAN21.7929.75248.9996665375

T47D21.2729.27256T47D257.19120894212.06323441010.0080221809

T47D21.2229.22256

T47D21.1729.19259.5736268262

HCLA19.7328.86560.2783750455HELA572.856956578560.97987191820.1064486888

HCLA19.6928.71519.1472536525

HCLA19.6829639.1452410376

BxPc320.9328.88247.2797002048BxPc3246.14490930291.96551549790.0079851966

BxPc320.9328.86243.8753274992

BxPc320.8728.82247.2797002048

Du14521.4129.5272.4787667081Du145272.758119867615.11911134150.0554304721

Du14521.5329.54257.7806208145

Du14521.3729.54288.0149720803

PC320.928.45187.402969081PC3179.04762627937.63117733590.0426209355

PC321.0928.52172.4458978388

PC320.9228.39177.2940119182

promega17.9928.61562.8894299756promega1563.289932930843.33943601050.0277232234

promega17.9928.641606.8282324926

promega18.0728.641520.1521363242

NTC29.18

NTC28.76

NTC29.06

7.26.12


hTERT1930.022076.58901461hTERT1723.5004455244306.95620038050.1781004474

hTERT18.9629.531520.1521363242

hTERT18.9529.571573.7601856389

CFPAC-120.8528.48198.0883191734CFPAC-1190.96475244526.16918975220.0323053845

CFPAC-120.9728.52187.402969081

CFPAC-120.8628.41187.402969081

ASPO-120.7729.18340.1435684087ASPO-1344.21955279411.35921882420.0329999233

ASPO-120.929.38357.0543785857

ASPO-120.7629.15335.4607113877

SW199021.5429176.0693527219SW1990157.223123556618.49431101330.1176309858

SW199021.929.19156.4979555446

SW199021.8728.99139.1020624033

SU.86.8620.7829.76504.9511447006SU.86.86489.038295795715.30969449880.031305717

SU.86.8620.8329.72474.4130876879

SU.86.8620.7229.65487.7506549985

promega18.0528.571468.3701739682promega1568.152375063687.13866776460.05556773

promega18.1728.841629.2587187301

promega17.9828.631606.8282324925

NTC29.36

NTC29.48

NTC29.03


CAPAN22.3530.49282.0877096644CAPAN286.154544202310.58684806330.0369969594

CAPAN22.330.42278.2041248067

CAPAN22.2730.49298.1717981359

T47D21.5529.89324.0336880564T47D314.577660188911.11510063990.0353334074

T47D21.5929.83302.334121326

T47D21.5129.82317.3651711844

HCLA19.8729.33704.2774108875HELA606.3349552146111.897036480.1845465704

HCLA19.2128.13484.3815151235

HCLA19.9529.25630.3459396326

BxPc321.2729.62326.2875205937BxPc3330.000057842237.92607422960.1149274775

BxPc321.2229.42294.0667788792

BxPc321.1629.69369.6458740538

Du14521.6730.21372.216964434Du145361.457430837716.45970824160.0455370587

Du14522.1230.65369.6458740538

Du14521.8530.27342.5094540252

PC321.1728.93216.7667999648PC3211.8978740377.17567369080.0338638305

PC321.1528.82203.6573398413

PC321.2328.98215.269482305

promega18.1629.051897.6523507515promega1859.692055712577.46166594190.0416529531

promega18.0628.961910.8515666674

promega18.1128.91770.5722497185

NTC30.44

NTC29.99

NTC30.26


hTERT19.3230.091746.1962583408hTERT1762.900642603251.18388829970.0290339042

hTERT19.0629.891820.3498110291

hTERT18.9229.671722.1558584396

CFPAC-121.0928.78206.5002903609CFPAC-1209.210459407514.0430483290.0671240261

CFPAC-121.1228.93224.4110646569

CFPAC-121.0728.69196.7200232049

ASPO-120.9429.65418.7658539816ASPO-1380.11413804633.49671384550.0881227781

ASPO-120.9229.42362.0386719675

ASPO-120.9529.44359.5378881889

SW199022.0629.31152.2185107203SW1990150.52368332754.8924895450.0325031213

SW199022.0129.28154.3434019444

SW199022.0229.2145.0091373179

SU.86.8620.9230.13592.2243501595SU.86.86563.952204771142.10561925970.0746616804

SU.86.8620.9429.95515.561241629

SU.86.862130.19584.0710225246

promega17.9328.71746.1962583408promega1673.115249101997.13470547390.0580561952

promega18.0328.641562.8894299756

promega18.1628.91710.2600589893

NTC30.04

NTC30.52

NTC30.17

Summary

Average T/ST/SStdevSW1990153.873403442140.9582465189

MCF10A_5368.508256609323.743607752140.9582465189CFPAC-1200.087605926440.5783488249

MCF10A_6288.1449064585Hs766T_3206.3368929189105.3062468698

MCF10A_5314.5776601889CAPAN259.901205540951.6153113047

PANC-1_3729.1956830603615.594575756698.5468177631BxPc3288.072483572637.1278275926

PANC-1_4553.0873062578ASPC-1362.1668454223.6725198561

PANC-1_1564.5007379516MIA PaCa-2_3472.853935067631.5785825413

MIA PaCa-2_3574.9231457911472.853935067689.569967863SU.86.86526.495250283498.5468177631

MIAPaCa-2_4407.3560551911PANC-1_3615.594575756689.569967863

MIA PaCa-2_1436.2826042206LNCAP_3296.213637120559.2945441696

Hs766T_3240.0898824174206.336892918931.5785825413PC3195.472750158223.2286329531

Hs766T_2177.5122330279Du145317.107775352762.7198842735

Hs766T_1201.4085633115T47D285.884434565527.8601465345

293T_31152.07833875011044.0398759024105.3062468698MCF10A_5323.743607752112.9016631206

293T_2941.697011141HELA589.595955896525.3813046395

293T_11038.3442778161293T_31044.03987590244.7372196161

LNCAP_3352.2452783944296.213637120551.6153113047hTERT1743.200544063852.9721330417

LNCAP_2250.6057232824promega1666.0624032022138.6731639772

LNCAP_1285.7899096848

CAPAN233.6478668795259.901205540937.1278275926

CAPAN286.1545442023

T47D257.1912089421285.884434565540.5783488249

T47D314.5776601889

HELA572.8569565785589.595955896523.6725198561

HELA606.3349552146

BxPc3246.1449093029288.072483572659.2945441696

BxPc3330.0000578422

Du145272.7581198676317.107775352762.7198842735

Du145361.4574308377

PC3179.0476262793195.472750158223.2286329531

PC3211.897874037

hTERT1723.50044552441743.200544063827.8601465345

hTERT1762.9006426032

CFPAC-1190.9647524452200.087605926412.9016631206

CFPAC-1209.2104594075

ASPC-1344.219552794362.1668454225.3813046395

ASPC-1380.114138046

SW1990157.2231235566153.87340344214.7372196161

SW1990150.5236833275

SU.86.86489.0382957957526.495250283452.9721330417

SU.86.86563.9522047711

promega1563.28993293081666.0624032022138.6731639772

promega1568.1523750636

promega1859.6920557125

promega1673.1152491019

142.8629553429142.8629553429

1339.4753393681339.475339368

258.339763908258.339763908

41.641917896541.6419178965

193.2370378882193.2370378882

83.671345743983.6713457439

33.656590606333.6565906063

2673.35753910132673.3575391013

72.180351320572.1803513205

128.8614791915128.8614791915

51.40997469951.409974699

Osteosarcoma Cell Line

Average T/S Ratio

MMQPCR RTL

Osteosarcoma Cell Line

Average T/S Ratio

MMQPCR RTL Normalized to Promega

24.707342273924.7073422739

13.43156807613.431568076

19.469099962619.4690999626

9.49463856259.4946385625

7.98555056457.9855505645

10.553695723410.5536957234

70.487506657270.4875066572

10.660485414910.6604854149

34.255158541434.2551585414

10.896046927310.8960469273

131.8325593756131.8325593756

18.552847167418.5528471674

23.471851393523.4718513935

11.115100639911.1151006399

61.077237842961.0772378429

24.22815051224.228150512

12.422508987712.4225089877

19.18336934519.183369345

13.295048497713.2950484977

2.06323441012.0632344101

60.979871918260.9798719182

1.96551549791.9655154979

15.119111341515.1191113415

7.63117733597.6311773359

43.339436010543.3394360105

306.9562003805306.9562003805

6.16918975226.1691897522

11.359218824211.3592188242

18.494311013318.4943110133

15.309694498815.3096944988

87.138667764687.1386677646

10.586848063310.5868480633

11.115100639911.1151006399

111.89703648111.89703648

37.926074229637.9260742296

16.459708241616.4597082416

7.17567369087.1756736908

77.461665941977.4616659419

51.183888299751.1838882997

14.04304832914.043048329

33.496713845533.4967138455

4.8924895454.892489545

42.105619259742.1056192597

97.134705473997.1347054739

40.958246518940.9582465189

40.578348824940.5783488249

105.3062468698105.3062468698

51.615311304751.6153113047

37.127827592637.1278275926

23.672519856123.6725198561

31.578582541331.5785825413

98.546817763198.5468177631

89.56996786389.569967863

59.294544169659.2945441696

23.228632953123.2286329531

62.719884273562.7198842735

27.860146534527.8601465345

12.901663120612.9016631206

25.381304639525.3813046395

4.73721961614.7372196161

52.972133041752.9721330417

138.6731639772138.6731639772

DoneTo be Done

OrganCell LineIdentification# times repeated

Pancreatic CancerSW1990 (CRL-2172)pancreas adenocarcionma derived from met. Spleen2COLO 357metastatic pancreatic adenocarcinoma

CFPAC-1 (CRL-1918)pancreas ductal adenocarcinoma; cystic fibrosis -derived from met. Liver2HPAC (CRL-2119)Pancreas Adenocarcinoma

Hs766T (HTB-134)pancreas carcinoma met. Site lymph node3Hs 700T (HTB-147)adencarcinoma derived from met. Site pelvis; thought to be from pancreas

Capan-1 (HTB-79)Pancreas adenocarcinoma derived from met. liver4HPAF-11 (CRL-1997)Pancreas Adenocarcinoma

BxPc3 (CRL-1687)pancreatic adenocarcinoma2Capan-2 (HTB-80)Pancreas Adenocarcinoma

ASPC-1 (CRL-1682)adenocarcinoma pancreas derived from met. Ascites2

MIA PaCa-2 (CRL-1420)pancreas carcinoma9

SU.86.86 (CRL-1837)pancreas ductal carcinoma derived from met. Liver2

PANC-1 (CRL-1469)pancreas duct epithelioid carcinoma7

Prostate CancerLNCAPprostate carcinoma met. Site left supraclavicular lymph node3normal prostate

PC3prostate adenocarcinoma met. Site bone2CA-HPV-10 (CRL-2220)Prostate Adenocarcinoma (HPV-18 infected)

Du145Prostate carcinoma derived from met. Brain2MDA-PCa-2b (CRL-2422)Prostate Adenocarcinoma derived from met. bone

PZ-HPV-7 (CRL-2221)Prostate Epithelium HPV-18 transformed

MPC-3prostate line subline of PC-3

BreastT47Dbreast ductal carcinoma derived from met. Pleural effusion2

MDA-MB-453Breast metastatic carcinoma derived from met. Site pericardial effusion2SK-BR-3 (HTB-30)Breast Adenocarcinoma mam gland derived from met. Pleural effusion

MCF7Breast Adenocarcinoma mam gland derived from met. Pleural effusion2Hs 578T (HTB-126)Mam Gland Breast Carcinoma

BT-20Breast Carcinoma2CAMA-1Breast Adenocarcinoma derived from met. Pleural effusion

MDA-MB-231Breast Adenocarcinoma mam gland derived from met. Pleural effusion

MDA-MB-157Breast Medullary Carcinoma


MDA-MB-175 VIIBreast epithelial ductal carcinoma derived from met. Pleural effusion

MDA-MB-134 VIBreast Ductal Carcinoma derived from met. Pleural effusion


MDA-MB-435Spreviously described as ductal carcinoma derived from met. Pleural effusion

MDA-MB-361 (HTB-27)Breast Adenocarcinoma mam gland derived from met. Brain

MDA-MB-436 (HTB-130)Breast Adenocarcinoma mam gland derived from met. Pleural effusion

HCC-1569Breast Mam gland TNM Stage 4 Grade 3 primary metaplastic carcinoma

HCC-1428 (CRL-2327)breast TNM stage 4 grade 4 from met. adenocarcinoma and pleural effusion

HCC-70breast duct TNM stage IIIA, grade 3 primary ductal carcinoma

HCC-1395Breast TNM Stage 1, Grade 3 Primary ductal carcinoma

HCC-1143Breast TNM stage IIA, grade 3 primary ductal carcinoma

HCC-1500Breast TNM stage IIB, grade 2 Primary ductal carcinoma

HCC-1954Breast TNM stage IIA, grade 3 ductal carcinoma

HCC-1419Breast TNM stage IIIA, grade 3 primary ductal carcinoma

HCC-1937Breast TNM stage IIB, grade 3 Primary ductal Carinoma

HCC-202 (CRL-2316)Breast TNM stage IIIA, grade 3 Primary Ductal Carcinoma

HCC-38 (CRL-2314)Breast TNM stage IIB, grade 3 Primary Ductal Carcinoma

AU-565 (CRL-2351)Breast Adenocarcinoma derived from met. malignant pleural effusion

BT-474Breast Ductal Carcinoma

BT-483Breast Ductal Carcinoma

BT-549 (HTB-122)Breast Ductal Carcinoma

ZR-75-1Breast epithelial ductal carcinoma derived from met. Ascites

ZR-75-30Breast epithelial ductal carcinoma derived from met. Ascites

EpitheliumMCF10AEpithelial Fibrocystic disease3

Cervical CancerHELAcervical adenocarcinoma2

Normal Embryonic Kidney293TAdenovirus SV40 T-antigen3

Normal PancreashTERTPancreas duct2

SkinA375Malignant Melanoma2

ColonHT-29colorectal adenocarcinoma2

SW-1417Dukes' type C, grade III, colorectal adenocarcinoma2

Normal LymphoblastoidUML21Human Female Lymphoblastoid, normal2

GM01247BNormal Male Lymphoblastoid4

GM10959Normal Female Lymphoblastoid4

PlacentaNormal Human Placenta4

# of times tested

OsteosarcomaSaos-21

CAL721

G2921

HOS1

Hu03-N11

NY1

OSACL1

U2OS1

143B1

HU091

MG63

YR

CB

DAR

LR

PH

hFOB

HOB1

CB_Normal

PH_Normal

Summary

T/S per runAvg T/SStdev

SW1990157.2231235566153.87340344214.7372196161SW1990153.87340344214.7372196161

SW1990150.5236833275CFPAC-1200.087605926412.9016631206

CFPAC-1190.9647524452200.087605926412.9016631206Hs766T206.336892918931.5785825413

CFPAC-1209.2104594075BxPc3288.072483572659.2945441696

Hs766T_3240.0898824174206.336892918931.5785825413CAPAN-1348.3156604118107.9668910621

Hs766T_2177.5122330279ASPC-1362.1668454225.3813046395

Hs766T_1201.4085633115SU.86.86526.495250283452.9721330417

CAPAN-1402.6348.3107.9668910621PANC-1616.873984660480.503816117

CAPAN-1470.8MIA-Paca-2632.3274185275196.40291216

CAPAN-1233.6478668795PC3195.472750158223.2286329531

CAPAN-1286.1545442023LNCAP296.213637120551.6153113047

BxPc3246.1449093029288.072483572659.2945441696Du145317.107775352762.7198842735

BxPc3330.0000578422T47D285.884434565540.5783488249

ASPC-1344.219552794362.1668454225.3813046395MCF71111.6327416437165.3303860688

ASPC-1380.114138046BT-201141.6235641208115.0388396795

MIA-Paca-2_1868.0632.3196.40291216MDA-MB-4531690.070104328299.1004679256

MIA-Paca-2_1841.9SW-14171621.212445466449.5878037412

MIA PaCa-2_3574.9231457911HT-292752.32136683444.7272649501

MIAPaCa-2_4407.3560551911143B430.43569661272.1803513205

MIA PaCa-2_1436.2826042206OSACL591.482656339533.6565906063

NS AVG->MIA PaCa665.5904878984HOS626.907587114741.6419178965

SU.86.86489.0382957957526.495250283452.9721330417NY2647.625877121883.6713457439

SU.86.86563.9522047711G2922917.5608678189258.339763908

PANC-1_3729.1956830603616.873984660480.503816117Hu03-N13058.3631863245193.2370378882

PANC-1_4553.0873062578Saos-23930.8974797217142.8629553429

PANC-1_1564.5007379516HUO92965.9060629489128.8614791915

NS AVG->PANC-1620.7122113719A3752028.0571120357184.1184233438

LNCAP_3352.2452783944296.213637120551.6153113047HELA589.595955896523.6725198561

LNCAP_2250.6057232824MCF10A323.743607752140.9582465189

LNCAP_1285.7899096848GM109592007.6212068965252.2422166958

PC3179.0476262793195.472750158223.2286329531UML212076.3838186924246.456037648

PC3211.897874037GM01247B3558.7135649449364.4695704283

Du145272.7581198676317.107775352762.7198842735293T1044.0398759024105.3062468698

Du145361.4574308377hTERT-HPNE1743.200544063827.8601465345

T47D257.1912089421285.884434565540.5783488249Human Placenta1105.4939158871236.5044224405

T47D314.5776601889Promega G152A1666.6900469216120.102683104

MCF10A_5368.508256609323.743607752140.9582465189

MCF10A_6288.1449064585

MCF10A_5314.5776601889

MDA-MB-4531760.11690.070104328299.1004679256

MDA-MB-4531620.0

MCF7994.71111.6327416437165.3303860688

MCF71228.5

BT-201060.31141.6235641208115.0388396795

BT-201223.0

HELA572.8569565785589.595955896523.6725198561

HELA606.3349552146

293T_31152.07833875011044.0398759024105.3062468698143B430.43569661272.1803513205

293T_2941.697011141OSACL591.482656339533.6565906063

293T_11038.3442778161HOS626.907587114741.6419178965

A3751897.92028.0571120357184.1184233438NY2647.625877121883.6713457439

A3752158.2G2922917.5608678189258.339763908

HT-292437.92752.3444.7272649501Hu03-N13058.3631863245193.2370378882

HT-293066.8Saos-23930.8974797217142.8629553429

SW-14171586.11621.212445466449.5878037412HUO92965.9060629489128.8614791915

SW-14171656.3293T1044.0398759024105.3062468698

UML211902.12076.3838186924246.456037648hTERT-HPNE1743.200544063827.8601465345

UML212250.7Human Placenta1105.4939158871236.5044224405

NS AVG->GM01247B3558.7135649449364.4695704283Promega G152A1666.6900469216120.102683104

NS AVG->GM109592007.6212068965252.2422166958

hTERT1723.50044552441743.200544063827.8601465345

hTERT1762.9006426032

NS AVG->Human Placenta DNA1105.4939158871236.5044224405

promega1563.28993293081666.6900469216120.102683104

promega1568.1523750636

promega1859.6920557125

promega1673.1152491019

NS AVG->G147A1669.2006217994

4.73721961614.7372196161

12.901663120612.9016631206

31.578582541331.5785825413

59.294544169659.2945441696

107.9668910621107.9668910621

25.381304639525.3813046395

52.972133041752.9721330417

80.50381611780.503816117

196.40291216196.40291216

23.228632953123.2286329531

51.615311304751.6153113047

62.719884273562.7198842735

40.578348824940.5783488249

165.3303860688165.3303860688

115.0388396795115.0388396795

99.100467925699.1004679256

49.587803741249.5878037412

444.7272649501444.7272649501

72.180351320572.1803513205

33.656590606333.6565906063

41.641917896541.6419178965

83.671345743983.6713457439

258.339763908258.339763908

193.2370378882193.2370378882

142.8629553429142.8629553429

128.8614791915128.8614791915

184.1184233438184.1184233438

23.672519856123.6725198561

40.958246518940.9582465189

252.2422166958252.2422166958

246.456037648246.456037648

364.4695704283364.4695704283

105.3062468698105.3062468698

27.860146534527.8601465345

236.5044224405236.5044224405

120.102683104120.102683104

Cell Line Name



8.17.12 Nano String Samples (Biological Replicates)Gabi's 7.9.12

No.NameT CtS CtT/SAverage T/SStdevT/S RatiosNameT CtS CtT/SAvg T/SStdevT/S

1A16.0626.551438.15155275571393.0954.46A1393.1A18.5529.121520.15213632421499.319795062520.78448648A1499.3197950625

2A15.9826.361332.5740599725A18.5529.081478.583496215

3A15.9126.371408.5548217751A18.4929.041499.2237526483

4B19.328.4548.7480128186559.049.73B559.0B20.329.3512540.963922040241.2027385272B540.9639220402

5B18.9728.1560.2783750455B20.3929.42522.7582083621

6B19.1628.31568.0995696987B20.9830.18588.1335577585

7C15.9627.954067.70686131013628.37380.66C3628.4C17.9729.583125.77885995123027.8464989652118.3681590627C3027.8464989652

8C15.5927.333420.5201179786C17.8629.443061.4512612044

9C15.7327.463396.892859656C17.8629.362896.3093757401

10D16.7227.561833.01134526051908.92120.61D1908.9D18.7129.51770.57224971851721.0181798364119.5059391171D1721.0181798364

11D16.6727.521845.760947427D18.6729.31584.7065533874

12D16.6927.692048D18.7829.61807.7757364033

13E17.0727.111052.78855849581082.6630.01E1082.7E19.229.06929.2996150448870.062841563351.383174227E870.0628415633

14E16.9327.011082.3864735348E19.3229.04843.357201682

15E17.0127.131112.8164992267E19.3829.09837.5317079632

16F18.827.97576.0299441607615.2035.05F615.2F20.7829.84533.7424695507499.76626211130.5902175843F499.766262111

17F18.7428.07643.5908477552F20.8129.7474.4130876879

18F18.828.09625.9918221783F20.9429.88491.1432290945

19NTC28.93NTC30.58

20NTC28.38NTC

NTC30.95

8.20.12 Nano String Samples (Technical Replicates)Gabi's 7.10.12

No.NameT CtS CtT/SAvg T/SStdevT/S RatiosNameT CtS CtT/SAvg T/SStdevT/S

1A17.9128.741820.34981102911901.57151.6999315231A1901.6A18.3729.352019.80457880241882.8176932866122.7286478959A1882.8176932866

2A17.9228.741807.7757364033A18.2929.141845.760947427

3A17.7728.792076.58901461A18.2629.061782.8875536305

4B19.4929.02739.2917481075816.20168.9300894395B816.2B20.130.131045.5164167242746.1538273767259.5520513891B746.1538273767

5B19.4728.92699.4126114583B19.8129.06608.8740428814

6B19.2829.261009.9022894012B20.0629.25584.0710225246

7C16.528.463983.99466460073841.69161.5899939716C3841.7C17.629.473743.05362013963736.9446557962168.1982910806C3736.9446557962

8C16.528.343666.022690521C17.4929.293565.7751072609

9C16.5728.493875.052120988C17.5229.453902.005239988

10D17.2928.422241.1135001822311.55122.0073256247D2311.6D18.3629.492241.1135001822088.9880877125133.3963608261D2088.9880877125

11D17.2428.372241.113500182D18.1529.142033.853430655

12D17.1728.432452.4363870596D18.5829.541991.9973323004

13E17.8228.091234.74721555531432.75284.1628063751E1432.7E19.0629.141082.38647353481036.50254098143.4444712479E1036.502540981

14E17.7328.081305.1500823749E19.0429995.9986661502

15E17.5828.361758.3420138245E19.3429.351031.1224832581

16F19.0228.7820.2955546518761.2752.6679309101F761.3F20.629.99670.9214227755606.609958712559.8424415321F606.6099587125

17F19.1928.68719.0757763779F20.9130.13596.3435962718

18F19.2228.76744.433928868F20.6529.76552.5648570903

19NTC29.33NTC30.96

20NTC28.98NTC29.21

21NTC27.4231.58?NTC29.59

NameT/S RatiosT/S AvgStdev

A1393.09347816781499.31979506251901.57152068081882.81769328661669.2006217994261.2298719104

B559.0419858543540.9639220402816.2022163223746.1538273767665.5904878984136.697608626

C3628.37327964823027.84649896523841.68982536993736.94465579623558.7135649449364.4695704283

D1908.92409756251721.01817983642311.55446247452088.98808771252007.6212068965252.2422166958

E1082.6638437524870.06284156331432.74643725161036.5025409811105.4939158871236.5044224405

F615.2042046981499.766262111761.2684199659606.6099587125620.7122113719107.4138136438

NameT/S AvgStdev

AG147A1669.2006217994261.2298719104promega

BMIA PaCa665.5904878984136.697608626

CGM01247B3558.7135649449364.4695704283Normal Male Lymphoblastoid

DGM109592007.6212068965252.2422166958Normal Female Lymphoblastoid

EH.P. DNA1105.4939158871236.5044224405

FPANC-1620.7122113719107.4138136438

Pancreatic Cancer

Prostate Cancer

Breast Cancer

Fibrocystic Disease

Cervical Cancer

Embryonic Kidney w/Adenovirus

Colorectal Adenocarcinoma

Malignant Melanoma

Lymphoblastoid Cell Line

Pancreatic Cell Line

Normal DNA

Osteosarcoma

Cell Line Name



72.1803513205

72.1803513205

33.6565906063

33.6565906063

41.6419178965

41.6419178965

83.6713457439

83.6713457439

258.339763908

258.339763908

193.2370378882

193.2370378882

142.8629553429

142.8629553429

128.8614791915

128.8614791915

105.3062468698

105.3062468698

27.8601465345

27.8601465345

236.5044224405

236.5044224405

120.102683104

120.102683104

DNAControls

Cell LineDateng/ulA260A280260/280260/230

COLO 3579.24.12102.292.0461.0631.922.17

HPAF-119.24.1228.190.5640.2921.932.31

HS700T9.24.12102.972.0591.0971.881.39

HPAC9.24.1225.750.5150.2621.971.41

RNA SEQ REVEALS A TRUNCATED RNA SEQ TRANSCRIPT IN SAOS2DIRECTION OF TRANSCRIPTION

ATRX INACTIVATION BY AN INTRACHROMOSOMAL REARRANGEMENTIN SAOS2

ATRX INACTIVATION BY A DELETION IN LEIOMYOSARCOMA





MAPPING A PATH TO CLINICAL RELEVANCE

HIGH COMPLEXITY = NEED FOR TUMOR SAMPLING.

PROGNOSTIC SIGNATURES (OF ANY TYPE).

EXPLORE SYNTHETIC LETHALITY WITH TSG DEFICIENCY.

DOES ALT CREATE A THERAPEUTIC OPPORTUNITY?

IDENTIFY SUBSETS WITH GAIN OF FUNCTION ALTERATIONS WHICH MAY BE TARGETED (e.g. MDM2).

IDENTIFY VULNERABILITIES WHICH ARE RELATED TOTHE DIFFERENTIATION STATE OF A GIVEN SARCOMA.

WEAK LINKS IN THE METASTATIC CHAIN.

COLLABORATORS

NCI Genetics Branch

Rizzoli Institute

Luca SangiorgiPiero Picci

TARGET PROJECT

Ching LauMark KrailoDon BarkauskasLisa TeotLaura MonovichJulie Gestier-FosterChand KhannaRichard GorlickTim TricheGreg ReamanNeyssa MarinaLee HelmanIrene AndrulisJay WunderJaime Guidry-AuvilDaniela GerhardMalcolm SmithSean DavisSven BilkeJack ZhuJosh WaterfallYevgeniy GindinRobert WalkerMarbin PinedaMark MackiewiczYuan JiangSo Young MoonPrincy FrancisNCI DCEG

Sharon SavageLisa Mirabello

***

Osteosarcoma incidence by country or region. Calendar period and the countries with national or regional registries are shown in Table I. The world rates include data from all countries and registries listed in Table I. Black triangles are male rates, grey circles are female rates. Number of osteosarcoma cases were available for all ages in this region but rate data were not; *7579 age group includes data from only East, West, and South European countries; 6569 and 7074 age groups do not include data from Asia, Latin America or North European countries.

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MINING COMPLEX SARCOMA GENOMES: LESSONS FROM OSTEOSARCOMA

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