Millicell® µ-Angiogenesis Assay Kits
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Transcript of Millicell® µ-Angiogenesis Assay Kits
New Millicell® µ-Angiogenesis Assay Kits
Studying how compounds affect angiogenesis, either to
promote or inhibit new capillary tube formation, can lead
to therapies affecting wound healing, tissue regeneration,
cardiovascular disease, stroke, tumor progression, and
more. The Millicell µ-Angiogenesis activation and inhibition
kits provide a powerful, quantitative platform for real-
time monitoring of changes in tubule formation with
unprecedented optical resolution.
Advantages•Continuous monitoring of cells
•Unique slide design eliminates any liquid
meniscus and out-of-focus areas
•Full well visualization at low magnification
•Optimized assay conditions
•Compatible with multichannel pipettes
•All-in-one slide used to grow, treat, and stain cells
•Miniaturized format reduces costs and waste by up to 80%
Optimized and easy-to-use slide-based assays with enhanced imaging capabilities
www.millipore.com/AngioSlides
Grow, treat, stain, and visualize cells all in one device
All your cells are in focus with Millicell µ-slides
Millicell µ-Slide vs. 96-Well Plate
No meniscus• Improved optics
• All cells in focus
• 10 µL inner well volume
• 15 wells
• Slide format
10x 10x
Depthof Focus
Unique well design eliminates meniscus
• Upper well contains
media/reagents
• Inner well contains
cells and/or matrix
Three simple steps, one easy µ-slide1. Addmatrixcomponents and incubate
2. Groworaddcells and incubate*
3. Addcalcein-AM and visualize
* Photos at right taken after cells were incubated 16-24 hours
Meniscus formation• Conventional optics
• Few cells in focus
• 100 µL well volume
• 96 wells
• Multiwell format
Cells w/oD, L-Sulforaphane
Cells w/D, L-Sulforaphane
Cells w/o PMA
Cells w/ PMA
Easy to quantify tube formation
µ-Slide kits promote robust tube formation
Value Pattern
0 Individual cells, well separated.
1 Cells begin to migrate and align themselves.
2 Capillary tubes visible. No sprouting.
Value Pattern
3 Sprouting of new capillary tubes visible.
4 Closed polygons begin to form.
5 Complex mesh-like structures develop.
µ-Angiogenesis Inhibition AssayThe angiogenesis inhibition kit includes ECMatrix™ to induce angiogenesis, the potent angiogenesis inhibitor
(D,L)-sulforaphane to act as a control, and calcein-AM to visualize the cells in real time.
µ-Angiogenesis Activation AssayThe angiogenesis activation kit includes fibrinogen and thrombin components to make the fibrin gel, the activation
control PMA (phorbol myristate acetate), and calcein-AM to visualize the cells in real time. (Left to right) Bright field,
calcein-AM, DAPI, and calcein-DAPI merge micrographs show robust tubule formation and lumen structure in the
presence of phorbol myristate acetate (PMA, a proangiogenic agent).
1µm 5µm 10µm 20µm 50µm
Withnostimulant LumenStructure
With50µg/mLPMAand1xITS
Description Qty/Pk Catalogue No.
NEW Millicell µ-Angiogenesis Inhibition Assay 1 MMA125
(5 slides, 75 microwells)
NEW Millicell µ-Angiogenesis Activation Assay 1 MMA130
(5 slides, 75 microwells)
Fibrin In Vitro Angiogenesis Assay (reagents only) 1 ECM630
(enough for 1 x 96 well plate)
In Vitro Angiogenesis Assay Kit (reagents only) 1 ECM625
(enough for 1 x 96 well plate)
Required Equipment/Reagents
Description Qty/Pk Catalogue No.
Accutase™ Cell Dissociation Solution 100 mL SCR005
EndoGRO™ Human umbilical vein endothelial cells (HUVEC) 1 vial (500,000 cells) SCCE001
EndoGRO LS complete medium 500 mL SCME001
EmbryoMax® ES Cell Qualified Ultra Pure Water, sterile H20 500 mL TMS-006-B
ORDERING INFORMATION
www.millipore.com
Millipore, Advancing Life Science Together, EmbryoMax, and Millicell are registered trademarks of Millipore Corporation. The M mark, ECMatrix, Accutase, ,and EndoGRO are trademarks of Millipore Corporation. Lit. No. PR2965EN00 03/10 BS-GEN-10-02933 Printed in U.S.A. © 2010 Millipore Corporation, Billerica, MA 01821 U.S.A. All rights reserved.
TO PLACE AN ORDER OR RECEIVE TECHNICAL ASSISTANCE In the U.S. and Canada, call toll-free 1 800-Millipore (1-800-645-5476)
In Europe, please call Customer Service:
France: 0825.045.645
Spain: 901.516.645 Option 1
Germany: 01805.045.645
Italy: 848.845.645
United Kingdom: 0870.900.46.45
For other countries across Europe and the world,
please visit www.millipore.com/offices.
For Technical Service, please visit www.millipore.com/techservice.