Microscopy with light and electrons

36

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The chapter gives the basic principles of microscopy. From "Electron Microscopy and Analysis" textbook by Peter J. Goodhew, John Humphreys and Richard Beanland. Courtesy of Taylor and Francis Books UK.

Transcript of Microscopy with light and electrons

Page 2: Microscopy with light and electrons

Contents (Note: Page numbers are as in the whole book so that the index remains valid)

Acronyms …………………………………………………….……………………… iii Preface ………………………………………………………………………………. iv

1 Microscopy with light and electrons 1.1 Introduction ………………………………………..… 1 1.2 Methods of image formation ………………………… 2 1.3 Pixels ………………………………………………… 3 1.4 The light-optical microscope …………….……….…. 4 1.5 Magnification …………………………………..……. 7 1.6 Resolution …………………………………………… 8 1.7 Depth of field and depth of focus ………………….… 12 1.8 Aberrations in optical systems ………………………. 14 1.9 Electrons versus light ………………………………... 16 1.10 Questions …………………………………………….. 19

Further reading ……………………………………………………………..……. 236 Answers ……………………………………………………………………………. 238 Index …………………………………………………………………………..……. 243

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 3: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 4: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 5: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 6: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 7: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 8: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 9: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 10: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 11: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 12: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 13: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 14: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 15: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 16: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 17: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 18: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 19: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 20: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 21: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 22: Microscopy with light and electrons
Page 23: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 24: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 25: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 26: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 27: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 28: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 29: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 30: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 31: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 32: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 33: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 34: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 35: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________

Page 36: Microscopy with light and electrons

Electron Microscopy and Analysis, Third Edition (electronic copy) by Peter J. Goodhew, John Humphreys, and Richard Beanland.

Reproduced by kind permission of Taylor and Francis Books UK under a Creative Commons Licence (BY-SA).

__________________________________________________________