Microscopy

Looking at the small stuff

Microscopes

“micro” means : Small

“scope” means: Watch/ look

It was actually developed first as entertainment for the rich

Light Microscope

Focuses visible light rays Can be used on live or dead specimens Dyes and fluorescent markers help for

viewing Magnify up to 1000X on the ones we have Examples:

Compound light microscope Confocal microscope

Light Microscope

Light Micrographs

Plant magnified

Human chromosomes

Confocal Microscope

Confocal micrograph

Spirogyra crassa

Maximum projectiongreen: Actin. Rhodamin, Label: Phalloidin. Actinred: Chloroplasts. AutofluorescenceImage acquired with the Leica TCS SP2

Electron Microscopy (EM) Focus beam of electrons on the

specimen Specimens must be dead Kinds

Transmission Electron Microscope (TEM) Cross-section of the material Most detailed of internal of cell

Scanning Electron Microscope (SEM) Surface of the specimen Shows texture, but less detail

TEM

TEM

SEM

SEM

Ant

Pollen Grains

Red Blood Cells

Compare cardiac myocytes

Maximum projection (overlay) of neonatal cardiac myocytes from rat.green: Myomesin, FITCred: Cadherin, Cy3blue: DNA (nuclei), DRAQ5Image acquired with the Leica TCS SL

Compare to TEM of myoctes on the wall.

Keen eyes

Light microscopes will not show you the nucleus of a cell or something the size of a virus!

Confocal microscopy uses brightly colored markers

TEM is detailed, flat, and black and white

SEM is 3D, textured, and black and white

Cited pictures Pollen grains (1000x magnification). Paul Fuqua. 2003.

unitedstreaming. 29 August 2006http://www.unitedstreaming.com/

Blood cells, red; microscopic view. U.S. Army. 2006.unitedstreaming. 29 August 2006http://www.unitedstreaming.com/

http://www-medlib.med.utah.edu/WebPath/HEMEHTML/HEME001.html

www.plymouth.ac.uk/upload/SCI/liverartery.jpg http://www.confocal-microscopy.com/WebSite/

pictures.nsf/(ALLIDs)/23C6531FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_app_img_i_cl.jpg

http://www.confocal-microscopy.com/WebSite/pictures.nsf/(ALLIDs)/23C6531FD6B41E6BC1256A7D0053F8CA/$FILE/drosoegg_app_img_i_cl.jpg

Cited Pictures

http://remf.dartmouth.edu/images/insectPart3SEM/image/31formicidae40(extra).jpg

http://mikes-machine.mine.nu/Mysterymoss/Micro3_DSCN6486.jpg

http://www.nims.go.jp/analysis/TEM.jpg http://www.nims.go.jp/analysis/SEM.jpg http://cdr.rfmh.org/NewFiles/Components/

images/confocal%20microscope.jpg http://131.229.114.77/microscopy/images/

chromosomes2.jpg http://www.biosci.ohio-state.edu/~plantbio/

osu_pcmb/images/Courses/PB101/plant_cell_tem.jpg