Microscopy and Imaging
Transcript of Microscopy and Imaging
Microscopy & Imaging Shared Resource(MISR)Michael Johnson, Ph.D.MISR Director
Jan Blancato, Ph.D.Cytogenetics and immunofluorescence
Peter Johnson, B.S.Manager
Susette C. Mueller, Ph.D.Consultant
MISR Provides:
• Routine and advanced cellular imaging and image analysis for tumor and cell biology studies.
• Guidance for tumor biology studies using quantitative imaging: subcellular changes in protein distribution.
• Access to advanced imaging instrumentation hardware and image analysis software including Metamorph and Volocity.
• Cytogenetics resources and services such as DNA FISH.
MISR Services:
• TIRF– Fluorescence, single
molecule– 150 nm adjacent to
coverslip– Multicolor fluorescence at
the plasma membrane (only!)
– Applications:• Membrane receptors• Exocytosis, endocytosis• Cell adhesions (TIRF/IRM)
TIRF(Total Internal Reflection
Fluorescence microscopy)
MISR Services:
• Confocal/ Multiphoton– Single cell or deep
tissue (up to 500 mm maximum)
– Live cell imaging– Second harmonic
detection of collagen fibers
– FRET/FLIM for protein-protein study
Fluoview FV300
Zeiss LSM510/META/NLO
MISR Services:• Spinning disk
confocal (live) imaging– Fast, 3D confocal
imaging of cells– Volocity 2D and 3D
image analysis– Multiwell, automated
image collection
• Routine fluorescence
MISR Training:
• Training of users through lectures, workshops, and individual instruction.
• Some of the equipment can be used by trained users. Technical assistance can always be requested.
MISR Contacts
• Mike Johnson, PhD Core Director as of 7/1/10
– x7-0217 (W414 Res. Bldg.)
• Jan Blancato, PhD Cytogenetics
– x7-0217 (W414 Res. Bldg.)
• Peter Johnson, B.S. Manager
– x7-1916 (W407/W408 Res. Bldg.)
• ZeissLSM and TIRF (E308 Res.Bldg)– x7-3168 (E308 Res. Bldg.)
Shared Resources are partially supported by the Georgetown Howard University Center for Clinical and Translational Studies (GHUCCTS) 1UL1RR031975-01
In your publications, remember to mention support provided from the GHUCCTS.