Microscopes. Field of View Fly’s Foot Surface of an Erasable Programmable Read-Only Memory Silicon...
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Transcript of Microscopes. Field of View Fly’s Foot Surface of an Erasable Programmable Read-Only Memory Silicon...
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Field of View
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Fly’s Foot
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Surface of an Erasable Programmable Read-Only Memory
Silicon microchip
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Eyelash hairs growing from the surface of human skin
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The surface of a strawberry
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Bacteria on the surface of a human tongue
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Human sperm (spermatozoa)
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Nylon hooks and loops of Velcro
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Household dust: includes long hairs of cat fur, twisted synthetic and woolen fibers,
serrated insect scales, a pollen grain, and plant and insect remains
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The weave of nylon stocking fibers
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The head of a mosquito
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Head louse clinging to a human hair
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Eight eyes (two groups of four) on the head of a tarantula
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Cut human hairs and shaving foam between two razor blades
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Cigarette paper the tobacco is rolled in
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Corroded surface of a rusty nail
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Mushrooms spores
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Clutch of butterfly eggs on a raspberry plant
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Calcium phosphate crystal
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Spider Skin
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Starch in a corn kernel
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Intestinal Bacteria
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Paramecium
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Volvox
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Hooke
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Eukaryotes
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FluidMoasicModel
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http://multimedia.mcb.harvard.edu/anim_innerlife.html
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Nucleus
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Nucleus
• Contains the genes that control the cell; localizing the cell’s DNA
• Nuclear envelope
- separates the nucleus contents from cytoplasm
- double membrane with pores to regulate what enters and exits nucleus
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Nucleus
• Nucleolus – densely packed RNA and proteins that makes up ribosomes
• Chromatin: threads of DNA that makes up the chromosomes and their associated proteins (histones)
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Endomembrane System• Membranes physically touch one another
or transfer membrane segments through tiny vessicles
• Includes:- Nuclear envelope- Endoplasmic reticulum- Golgi apparatus- Lysosomes- Peroxisomes- Cell membrane
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ER + Ribosomes
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Endoplasmic Reticulum
• Membranous labryinth extending from the nuclear membrane
• Made of tubes and sacs called cisternae
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Rough ER: ribosomes stud the cytoplasmic surface
• Many types of specialized cells secrete proteins made by the rough ER which are moved in transport vesicles
• As the polypeptide chain grows from the ribosomes, it goes into the ERs cisternal space and folds into higher levels
• Some proteins (glycoproteins) are covalently bonded to carbohydrates or are embedded in newly synthesized lipid membranes
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Smooth ER: cytoplasmic surface lacks ribosomes
• Function:
- synthesis of phospholipids and steroids
-carbohydrate metabolism,
-detoxification of drugs/poisons
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Ribosomes
• Sites where cells assemble proteins according to genetic instructions
• Free ribosomes are suspended in the cytoplasm which make proteins that will function in the cytosol
• Bound ribosomes are attached to the endoplasmic reticulum which make proteins that be included in membranes or transported outside the cell
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Golgi Bodies
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Golgi Apparatus
• Flattened, inner-connected membranous sacks with cisternae
• Products of ER are modified, stored, and shipped via transport vesicles
• Secretory vesicles have “docking sites” that recognize the surface of specific organelles
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Lysosome
• Membrane-enclosed bag of hydrolytic enzymes that digests macromolecules
• Works best in an environment with pH = 5
• Amoeba eat via phagocytosis = engulf food then digest as the lysosome fuses to it
• Usually only found in animals
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Animation: Lysosomes
Lysosome
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Peroxisome
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Peroxisome
• Contains enzymes that transfer hydrogen from various substrates to oxygen, producing H2O2
• The H2O2 is then converted into water
• Helps breakdown fats to be used in mitochondria for energy
• Detoxify alcohol in the liver
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Mitochondria
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Mitochondria
• Site of cellular respiration which generates ATP from sugars and fats
• Have ribosomes and small amounts of DNA for its own protein synthesis
• 100s-1000s per cell which correlate to the metabolic activity of the cell
• Enclosed in phospholipid bi-layer with inner foldings called cristae
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Chloroplasts
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Chloroplasts• Convert solar energy into chemical energy
by absorbing sunlight and using it to synthesize glucose from CO2 and H2O.
• Plastids: specialized organelles in plants- Leucoplasts: store starch in roots and tubers- Chromoplasts: enriched in pigments that give plants their colors- Chloroplasts: contain the green pigment cholorphyll
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• What organelle is taking up most of the space in this plant cell?
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Vacuole
• Central vacuole: stores water, organic compounds, ions, wastes, and hydrolytic enzymes
• Tonoplast: membrane surrounding plant vacuole
• Vacuoles and vesicles are both membranous sacs, but vacuoles are larger
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Cytoskeleton
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Cytoskeleton• Network of fibers that allow for material
movement within the cell• microtubules – larger filaments that help
• move organelles during cell division • form hair-like extensions on cell surface for
movement (cilia or flagella)• Centrioles – rings of microtubules which form
spindle fibers to aide in cell division
• Microfilaments (actin) – smaller filaments that maintain shape and aid in muscle contraction
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Centriole
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Flagella
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Cell Wall
• Made of cellulose (polysaccharide) not digested by most animals
• Rigid structure that supports and protects plant cell by preventing the excess uptake of water- turgor pressure from vacuole assists in support
• Allows movement of materials in/out of cell through plasmodesmata (channels)
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Cytoplasm
• Cytosol: semi-fluid medium in which organelles are suspended
• Mostly water but also contains ions, sugars, amino acids, RNA, and ATP (cell energy)
Harvard Cell Animation/
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Plasmolyzed Flaccid Turgid
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Exocytosis