Microscope Control with LabVIEW and IMAQ Vision · Microscope Control with LabVIEW and IMAQ Vision...
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Transcript of Microscope Control with LabVIEW and IMAQ Vision · Microscope Control with LabVIEW and IMAQ Vision...
Microscope Control with LabVIEW and IMAQ Vision
National Instruments Scientific Imaging Symposium
Morten Jensen NI Consulting Services
Agenda
• National Instruments Consulting Services• Microscope Control – First Steps• Microscope Start-up Kit• Future Microscope Kits (Compact Vision System)• Vision Builder for Automated Inspection• Q&A
What is National InstrumentsConsulting Services?
• Design consulting & reviews • Proof of concepts• Benchmarking • Feasibility studies • Startup assistance
• “Meet the Experts”• Cooperative risk management • Close support during development • Specialized training
Microscope Control – First Steps: Cell CountingFeatures: • IMAQ/Motion/DAQ Integration
• XY Axis Control (Manual & Auto)• Display Results and Center FOV• Automatic FOV Image Stitching
Cell Counting / Analysis• Threshold image• Over 50 parameters available for analysis
QuantitySizeLocationArea
Microscope Control – First Steps
Displayed:• Medical Design and
Manufacturing (’01, ’02, ‘03)• PittCon (’02, ‘03)• NI Week• 3 Shows in Europe
Next Step: Microscope Start-up Kit
Camera
ControllerUnit
PXI and LabVIEW Vision
Microscope
RS-232
C-Mount
NI-IMAQ
Features• Low-cost
Microscope Monitoring and Control Tool
• Accurate Sample Analysis Capability
• Intuitive Graphical Interface
Microscope Start-up Kit
Recipe:• PXI Digital Framegrabber• LabVIEW and NI IMAQ
Vision• DVC filter wheel camera • Olympus BX61
Microscope and BX-UCB Microscope Controller Unit
Microscope Start-up Kit
• Easily Modified Software Architecture
• Open Source Code Based on LabVIEW Graphical Programming Environment
• Can be Upgraded Using Standard PCI or PXI-Based Technology
• Located on NI Developer Zone
Microscope Start-up Kit
• Login to Microscope and Camera Initialization
Microscope Start-up Kit
• Microscope Serial Control Through Controller Unit
Microscope Start-up Kit
• Set Objectives if changes in Front Panel
Microscope Start-up Kit
ApplicationsMonitoring and control for:• Microscope Functions
– Cell Counting– Cell Analysis– Cell Imaging
• Other Microscope Applications
Microscope Start-up Kit
• Close Camera and Microscope Session.
Microscope Start-up KitDisplayed: • Neuroscience Show
’02, ’03• Pittcon ‘03
Microscopy with National Instruments Products
Image courtesy of University of Utah
Example:• Quantitative Analysis of
Cytoskeletal Fiber Orientation• LabVIEW and IMAQ Vision
Pioneering new types of Image Processing Tools for Biomedical Research
Future Microscope Kits – Compact Vision SystemNI 1450 Series
Model 1454 1455
Ram/Flash 128/32 128/128
Speed (MIPS) 883 1436
Sensor Size You choose You choose
SoftwareConfigurable or programmable
Configurable or programmable
Digital I/O 29 DIO 29 DIO
Video out Yes Yes
Operating Temp. 0-55 C 0-55 C
• Stand-alone System with Embedded Analysis Functionality
• Based on ProvenLV RT Technology
• IEEE 1394.a acquisition – Supports any DCAM
compliant camera– 640 x 480 @ 100 fps– 1024 x 768 @ 15 fps
• Industrial ruggedness – No fan or external vents – Shock resistant
NI 1450 Series Connectivity
Configurable I/O10/100 Base T
RS-232
VGA Output
IEEE 1394.a
Digital I/O
Vision Builder for Automated Inspection – Configuration InterfaceMain Window Inspection StepsInteractive Help Inspection Diagram
Window
Vision Builder for Automated Inspection• Ease of Use
– Prototyping and configuration environment
• Fast Development– Deployable user
interface included– Decision-making capabilities– Digital and
Serial I/O capabilities
• Evaluation Software Available
• Generates LabVIEW code!
Thank You!
Questions?