Microfluidics for Single-Cell Sample Preparation: Market & Technology Analysis

March © 2016

From Technologies to Market

Microfluidics for Single-Cell Sample

Preparation:

Market & Technology Analysis

Single Molecule & Single Cell Analysis Conference 2016

From Technologies to Market

Sébastien Clerc

Yole Développement

[email protected]

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PRESENTATION OUTLOOK

25 + 5 mins

• Yole Développement Introduction

• Sample preparation issues

• Single-cell challenges

• The rise of microfluidics for single-cell

• What’s next?

• Q&A

©2016 | www.yole.fr | Single Molecule & Single Cell Analysis Conference 2016

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Yole Développement

Market, technology and strategy consulting


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4

MEMS &

Sensors

LED

Compound

Semi.

Imaging Photonics

MedTech

Manufacturing

Advanced Packaging

Batteries / Energy

Management

Power

Electronics

FIELDS OF EXPERTISE

Yole Développement’s 30 analysts operate in the following areas


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OUR GLOBAL ACTIVITY


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Microfluidics for Single-Cellsample preparation


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SAMPLE PREPARATION ISSUES

Single-cell sample

preparation issues is one of the focus of our latest

report

Molecular

DiagnosticsSingle-cell

Next-Generation

Sequencing


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WHY SINGLE-CELL?

Single cells behave

differently than cell

populations

Objective: understanding the molecular mechanisms at the cellular level:

• Immune system response to an infection

• Cell response to a drug

• Genetic changes

• Tumor characteristics

• …

Problem: heterogeneity among cell populations => different behaviors…


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WHY SINGLE-CELL?

Cell-to-cell variability

Single-cell analysis

enables the identification

of cell heterogeneity


Let’s compare two cell populations…

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WHY SINGLE-CELL?


3

3

4

3

43

4

Average = 3.4






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WHY SINGLE-CELL?


3

3

4

3

43

4

3

3

63

3

33

Average = 3.4 Average = 3.4






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WHY SINGLE-CELL?


3

3

4

3

43

4

3

3

63

3

33

Average = 3.4 Average = 3.4Conclusion: all cells have a parameter value of 3 or 4…

…Right?

A single-cell analysis would have enabled the detection of the

orange tumor cell, whereas an average measurement within the

population could not.






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WHY SINGLE-CELL?

Through the identification of small but critical

changes from one cell to another,

single-cell analysis opens new life

science opportunities

Single-cell genomics

and proteomics

Cancer research

Personalized medicine


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SINGLE-CELL ISOLATION TECHNOLOGIES

What we call “classic” methods

FACS Acoustic tweezers

Optical tweezersLaser Capture

Microdissection


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SINGLE-CELL ISOLATION TECHNOLOGIES

Increasing emergence of microfluidic

methods

Droplet cell encapsulation

(picture: Dolomite Microfluidics)Dielectrophoresis cell isolation

by Silicon Biosystems

Microstructure cell trapping

by Fluidigm

Significant advantages of microfluidic technologies:

• Precise environment control (easier to mimic in-vivo conditions)

• Small samples and low reagent volumes

• High-throughput (parallelization)

• Low shear stress

• Low to medium cost per experiment

• Enable automation

• Reduced risk of contamination using disposables


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THE RISE OF MICROFLUIDICS FOR SINGLE-CELL

The market is booming

We’ve reachedan inflection

point

CAGR 2016 - 2020: 18%

CAGR 2014 - 2016: 97%


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A very active field

A lot of thingsrecently

happened or were announced


WaferGen launched its ICELL8 single-cell system (Sept. 2015)

NanoCellect raised $1.75M to begin production of the WOLF system (Feb.2016)

Fluidigm announces the new Polaris™ system (Feb. 2015)

Bio-Rad and Illumina announce a collaboration to develop a

comprehensive solution for single-cell genomics (Jan. 2016)

Dolomite launched a microfluidic chip for the creation of single-cell libraries (Nov. 2015)

Cytena raised $1.1M (Apr. 2015)

Sphere Fluidics raised $7M for the development of the Cyto-Mine® system (Feb. 2016)

WaferGen raised $17.25M (Oct. 2015)

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THE RISE OF MICROFLUIDICS FOR SINGLE CELL

Why this sudden explosion?

A long path ledto this market

explosion

Unsatisfying

methodsGrowing demand

Years of

R&D work

Technology

is now ready

Investors

confidence

1 2 3

456

Acquisitions

Startups

are created

CollaborationsNew solutions

arrive


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Microfluidic players (non-exhaustive list)

The competition is strenghtening

TimeToday


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CURRENT OR FUTURE MICROFLUIDIC SOLUTIONS FOR SINGLE-CELL SAMPLE PREPARATION

(non exhaustive list)

Fluidigm C1™

Single-Cell Auto

Prep System

(microstructures)

Silicon Biosystems DEParray™

Cartridge (dielectrophoresis)

Sphere Fluidics Cyto-Mine®

(picodroplet technology)

Cytena cy-Clone Single-Cell Printer

Celsee Diagnostics

(formerly DeNovo Sciences)

Celsee PREP400 liquid biopsy sample

preparation platform

NanoCellect WOLF Cell

Sorter and its microfluidic chip

WaferGen ICELL8

Single-Cell System

(nanodispensing)


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WHAT’S NEXT?

Opportunities for microfluidic players at each level of the supply chain

Microfluidicplayers all alongthe supply chaincan benefit from

single-celldevelopments


Fabs System developersDownstream

applications providers

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WHAT’S NEXT?

Technical limitations

Microfluidicslook promising

and rapidlyimprove

Droplet technologies

Microstructured traps

High throughput

Low precision (Poisson distribution)

High precision

Low throughput

Precision

Throughput

?Improvement/combination

of existing?

New technology

development?


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WHAT’S NEXT?

Evolution of the competitive environment

Who will taketomorrow’s

largest marketshare?

?2020


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SUMMARY AND CONCLUSION

• Microfluidic technologies are now ready to address single-cell challenges

• However, significant improvements are still required

• High double-digit growth rate

• Numerous new entrants and new solutions

• New opportunities for the whole microfluidics supply chain

• The cards will be redistributed among the players


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THANKS FOR YOUR ATTENTION!

Please take a look at our websites www.yole.fr and www.i-micronews.com

To get a complete copy of the presentation, please feel free to come to me (Booth #13) for business cards exchange

Microfluidic Technologies for Sample Preparation 2015Released in Nov. 2015 – More info on www.i-micronews.com

Microfluidic Applications 2015Released in June 2015 – More info on www.i-micronews.com

Some slides from this presentation were extracted from the following reports


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Yole Développement

Market, technology and strategy consulting


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4 BUSINESS MODELS

o Consulting and Analysis

• Market data & research, marketing analysis

• Technology analysis

• Strategy consulting

• Reverse engineering & costing

• Patent analysis

www.yole.fr

o Reports

• Market & Technology reports

• Patent Investigation and patent infringement risk analysis

• Teardowns & Reverse Costing Analysis

• Cost Simulation Tool

www.i-Micronews.com/reports

o Financial services

• M&A (buying and selling)

• Due diligence

• Fundraising

• Maturation of companies

• IP portfolio management & optimization

www.yolefinance.com

www.bmorpho.com

o Media

• i-Micronews.com website

• @Micronews e-newsletter

• Communication & webcast services

• Events

www.i-Micronews.com


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A GROUP OF COMPANIES

Market,

technology and

strategy

consulting

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M&A operations

Due diligences

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Innovation and business maker

www.bmorpho.com

Manufacturing costs analysis

Teardown and reverse engineering

Cost simulation tools

www.systemplus.fr

IP analysis

Patent assessment

www.knowmade.fr


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SERVING THE ENTIRE SUPPLY CHAIN

Our analysts provide market analysis,

technology evaluation,

and business plan along the entire

supply chain

Integrators and

end-users

Device

makers

Suppliers: material,

equipment, OSAT,

foundries…

Financial investors,

R&D centers


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CONTACT INFORMATION

Follow us on

• Consulting and Specific Analysis

• North America: Steve LaFerriere, Director of Northern America Business DevelopmentEmail: [email protected]

• Japan & Asia: Takashi Onozawa, Representative Director, Yole KKEmail: [email protected]

• RoW: Jean-Christophe Eloy, CEO & President, Yole DéveloppementEmail [email protected]

• Report business

• North America: Steve LaFerriere, Director of Northern America Business DevelopmentEmail: [email protected]

• Europe: Fayçal El Khamassi, Headquarter Sales Coordination & Customer Service Email: [email protected]

• Japan & Asia: Takashi Onozawa, Representative Director, Yole KK.Email: [email protected]

• Korea: Hailey Yang, Business Development Manager, Korean OfficeEmail: [email protected]

• Taiwan: Mavis Wang, Business Development DirectorEmail: [email protected]

• Financial services

• Jean-Christophe Eloy, CEO & PresidentEmail: [email protected]

• General

• Email: [email protected]


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Microfluidics for Single-Cell Sample Preparation: Market & Technology Analysis

Technology

Transcript of Microfluidics for Single-Cell Sample Preparation: Market & Technology Analysis