Microbiology training module--finger dab& higeine
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Transcript of Microbiology training module--finger dab& higeine
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Quality Control-MicrobiologyTraining on hygiene & finger dab test
Sreenath.S
Site Microbiologist
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Over view of finger dab & hygiene• Cleanliness of the personnel
• Maintenance of hygiene
• Proper hand sanitization
• Disinfectant cleaning
• Hand Swabbing
• Use of right sanitizer
• Growth of organisms
• Educating personnel's
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Why Is Hand Hygiene Important?
Hands are the most common mode of pathogen
transmissionHand washing is an extremely
important step
Hand washing significantly removes the bacterial flora on the skin
Hand washing protects you and your patient from the bacterial flora (from
each other) from becoming skin residents
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Hand Hygiene Definitions:
Hand washing−Washing hands with plain
soap and water• Antiseptic hand wash
−Washing hands with water and soap or other detergents containing an antiseptic agent
• Alcohol-based hand rub−Rubbing hands with an
alcohol-containing preparation (used after hand washing)
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When to Wash:• Visibly dirty
• After touching contaminated objects with bare hands
• Before glove placement and after glove removal
• Visibly dirty
• After touching contaminated objects with bare hands
• Before glove placement and after glove removal
Hand washing before and after each operation is the single most important hygiene measure for reducing or preventing the spread of contamination
Hand washing before and after each operation is the single most important hygiene measure for reducing or preventing the spread of contamination
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Rings and Jewelry
• Hand jewelry should not be
worn as it may tear gloves
and harbor bacteria
− Studies have demonstrated that
skin underneath rings is more
heavily colonized than
comparable areas of skin on
fingers without rings
− the more rings worn, the greater concentration of organisms
• Hand jewelry should not be
worn as it may tear gloves
and harbor bacteria
− Studies have demonstrated that
skin underneath rings is more
heavily colonized than
comparable areas of skin on
fingers without rings
− the more rings worn, the greater concentration of organisms
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Fingernails
• Can affect the integrity of gloves
• Can also harbor bacteria
• Keep fingernails SHORT!
− Avoid artificial nails
• Can affect the integrity of gloves
• Can also harbor bacteria
• Keep fingernails SHORT!
− Avoid artificial nails
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Wash hands (or use and alcohol based rub) before glove placement and after glove removal..
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Hand Hygiene: Frequently Missed AreasHand Hygiene: Frequently Missed Areas
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Cleanliness of the personnel’s• Always clean your hands before entering into manufacturing area
and where ever it is to be followed.
• Disinfectant the gloves during operation.
• Personnel Hygiene
• Illness
− Staff with illness or open lesions should not handle starting materials, intermediates or finished products
• Adverse conditions
− operators trained to recognize risks
− willingness to report illness to the area supervisor
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Personnel Hygiene
Health examinations
–On recruitment for direct operators , repeated on regular basis
•Training - check
– induction training for new operators includes basic personal hygiene training
–written procedures - to wash hands before entering a manufacturing area
–signs in changing rooms to reinforce hand washing
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Personnel Hygiene
•Contact between product and operator
avoid direct contact
if direct handling unavoidable, gloves should be worn
check glove disinfection (for sterile
production) and disposal
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Personnel Hygiene
•Clothing and changing facilities
−check changing rooms (hand washing, towels or hot air hand dryers)
−check if used clothing stored in separate closed containers while awaiting cleaning
−laundering of clean area clothing
must be to SOP and in
appropriate facility
−check for procedure for sterilizing
and storing clothing for use in
sterile area
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FINGER DAB TESTFINGER DAB TEST• Frequency:
• Once in six months
• Sampling plan: (Random sampling)
• Persons working in the manufacturing area are selected randomly from each section on the day of the test.
• Limits:
• For Bacteria: Standard limit: NMT 100 cfu/Personnel
• Alert limit: NMT 50 cfu/ Personnel.
• Action limit: NMT 75 cfu/ Personnel.
• Fungi: Should be absent
• Pathogens: Should be absent
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FINGER DAB TEST DEMOFINGER DAB TEST DEMO
SWAB TAKEN BEFORE HAND WASH SAMPLE UNDER MICROBIAL
TESTING
AFTER INCUBATION COLONIES ARE IDENTIFIED
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FINGER DAB TEST DEMOFINGER DAB TEST DEMO
SAMPLE TAKEN AFTER HAND SANITIZATION
SAMPLE UNDER MICROBIAL
TESTING
AFTER INCUBATION NO COLONIES ARE IDENTIFIED
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Taking off the first glove:
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2.
3.
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4.
5.
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Taking off the second glove:
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2.
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3.
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5.
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Disposal:
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THANK You………THANK You………
Any Query ????????