Microbiology
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Transcript of Microbiology
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Microbiology• Study of microscopic organisms • Unicellular, multicellular, or acellular • Different fields of study – Virology – study of viruses– Mycology – study of fungi– Parasitology – study of parasites, hosts
and relationships– Bacteriology – study of bacteria– Protozoology – study of protozoa– Nematology – study of nematodes– Phycology – study of algae
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What is a Microbe • Smaller than 0.1mm• Includes archaebacteria, bacteria, protozoans, fungi, viruses
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Scientific names• Every organism that has been
discovered is given a scientific name
• Founded by Carolus Linnaeus (1735)
• Genus species• G. species• Genus species • Two organisms sharing a common
genus are related
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Scientific Names
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Examples• Klebsiella pneumoniae• Clostridium difficile• Escherichia coli• Staphylococcus aureus– MRSA
• Streptococcus pyogenes– Flesh eating disease
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Why study Microbiology• Microbes are related to all
life– In all environments – Industrial uses
(fermentation, antibiotics, probiotics)
– Related to life processes (food web, nutrient cycling)
– Pathogenic (infectious diseases)
– Most of our problems are caused by microbes
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EID’s• Emerging infectious diseases– Weapons of mass destruction– New evolutionary features– Response to man encroaching on the environment
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Patterns of Disease• Endemic – disease is
present in low levels of a population at any time
• Outbreak – sudden increase in prevalence of a particular disease
• Epidemic – sudden increase in prevalence on a national scale
• Pandemic – rapid spread of pathogen throughout the world (HIV, bubonic plague, cholera, tuberculosis, HIV/AIDS, influenza)
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Patterns of Disease
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Biotechnology• Use of living systems and
organisms to develop useful products– Biodegradable plastics,
vegetable oil, biofuels (85% ethanol)
• The use of biological information to make things or improve the human condition– Pharmacogenomics – study of
how the genetic inheritance of an individual affects his/her body’s response to drugs
– Gene therapy
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Prokaryotic Cells• Include Bacteria and Archaea• Lacks a nucleus and other
membrane bound organelles• Contain ribosomes• Pili – hair like structures used to
attach to other cells or objects• Flagellum – tail like extension
for motorboating (propeller)• Cell wall• Nucleoid – chromosomal DNA• Plasmids – small circular pieces
of DNA• Capsule – composed of
polysaccharides, protects cell against phagocytosis
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Common Shapes of Prokaryotes • Coccus (Round)– N. meningitidis – meningitis
• Bacillus (Rod)– B. anthracis - anthrax
• Diplococci (Pair)– N. gonorrhoea – gonorrhoea
• Streptococci (chain)– S. pyogenes – scarlet fever
• Spirochete (Spiral)– T. pallidum – syphilis
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• Cell walls– Composed of
peptidoglycan • Reproduce
Asexually– Binary fission
• Make energy, use organic chemicals, inorganic chemicals, or photosynthesis
Bacteria
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Archaea• Lack peptidoglycan in cell wall• Reproduce via Binary Fission• Live in extreme environments• Methanogens
– Live in environments that lack oxygen
– Produce methan gas– Live in landfills
• Extreme halophiles– Live in environments that love salt – Dead Sea, Great Salt Lake
• Extreme thermophiles– Live in environments of extreme
heat – Volcanic vents deep in the ocean
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Binary Fission• Asexual
Reproduction
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Conjugation• Transfer of genetic
material between bacterial cells
• Creates genetic diversity
• Antibiotic resistance
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• Unicellular eukaryotes• Animal-like – consumers
• Plant- like– producers
• Motility– Ciliates – Flagellates – Amoeboids– Sporozoan
Protozoa (Eukaryotic Cells)
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Animal-like Protists • Ciliates – Move using short, thread like cilia that extend from
plasma membrane – Sweep food into their bodies
• Balantidium coli– Causes Balantidiasis
• Paramecium
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Balantidium coli• Trophozoite – Active feeding stage – Binary fission
producing cysts – Invade the wall of large
intestine– Cannot survive outside
human host• Cyst– Dormant, resting stage– transmitted to humans – Survive outside human
host
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Animal-like Protists• Flagellates– Use a whipping action (propellor) of tail like flagella
• Giardia lamblia– Causes Giardiasis (beaver fever, backpackers disease)– Reproduce in the small intestine (binary fission)
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Giardia lamblia• Trophozoite – Invade wall of large
intestine– Cause severe
diarrhea• Cyst– Dormant, resting
stage– transmitted to
humans – Survive outside
human host
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Animal-like Protists• Amoeboids• Pseudopods –
extension of cytoplasm creating foot-like structure for movement and catching food
• Entamoeboa histolytica • Causes Amoebiasis
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Entamoeba histolytica • Trophozoite– Invade extraintesinal
sites (liver, brain, lungs)– Cause liver and brain
abscess• Cyst – Ingested by humans
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Fecal Oral Route• Pathogens in fecal
matter pass from one host and is introduced to the oral cavity of another
• Excystation /Encystment– Transforming from cyst to
trophozoite and back to cyst
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Animal-like Protists• Sporozoans– Cannot move on their own
(nonmotile)– Reproduce both sexually
(gametes) and asexually (mitosis)
– Parasites – Transmitted through vectors
(insects)• Plasmodium vivax– Causes malaria (most common) – Transmitted by female
anopholes (mosquito)
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Plasmodium vivax • Mosquito bites
– Infects human with sporozoites
• Sporozoites infect liver cells– Develop into merozoites
• Merozoites infect RBC’s– Continue to infect RBC’s – Some develop into
gametocytes • Mosquito bites infected
human– Gametocytes fertilize,
develop into oocyst• Oocyst ruptures releasing
sporozoites
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• Eukaryotes• Cellulose cell walls• Use photosynthesis for
energy (primary producers)
• Produce molecular oxygen and organic compounds
Plant-like Protists (Algae)
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Plant-like protists • Dinoflagellates– Marine plankton (flagella)– Red tide
• Algal blooms produce dinotoxins • Toxic to humans
• Diatoms – Phytoplankton– Cell wall is made up silica
• Euglenoids – Contain chloroplasts – Also feed by phagocytosis
• Green Algae – Macroscopic seaweeds
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• Eukaryotes• Chitin cell walls• Use organic chemicals for
energy (consumers)• Moulds – consist of masses of mycelia,
which are composed of filaments called hyphae
– Reproduce asexually (spores) • Yeasts – Round shape – Reproduce asexually
(budding)
Fungi
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• Acellular – non-living • Consist of DNA or RNA
core• Core is surrounded by
a protein coat• Coat may be enclosed
in a lipid envelope• Viruses are replicated
only when they are in a living host cell
Viruses
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Multiplication of Viruses• Adsorption• Penetration• Uncoated• Synthesis• Replication and
Protein Production
• Assembly • Release
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Universal precautions set up by CDC• Use gloves,
gowns, masks and goggles
• Minimize risk of needle sticks
• Disinfections procedure
• Preventative treatment after exposure
• Reduce risk• Treat all patients
the same