Microbiological & Immunological Aspects of Dental...
Transcript of Microbiological & Immunological Aspects of Dental...
![Page 1: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/1.jpg)
Microbiological & Immunological Aspects of Dental caries
Hong-Sen Chen. DDS. MS. Ph.D
![Page 2: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/2.jpg)
Introduction and overview
![Page 3: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/3.jpg)
![Page 4: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/4.jpg)
![Page 5: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/5.jpg)
![Page 6: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/6.jpg)
![Page 7: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/7.jpg)
ETIOLOGICAL FACTORS IN DENTAL CARIES
Caries
NoCaries
NoCaries
NoCaries
NoCaries
Plaquemicro-organisms
Time
Teeth Diet(sugar)
Saliva
Saliva
Saliva
Saliva
![Page 8: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/8.jpg)
The theory
Acidogenic theoryProteolytic theoryAcidogenic - proteolytic theoryProteolysis – chelation theory
![Page 9: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/9.jpg)
The infectious nature of dentalcaries in experimental animals
![Page 10: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/10.jpg)
![Page 11: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/11.jpg)
Germ-free animals
Normal bacterial flora No bacteria
Sugars
Caries
Sugars
No Caries
Animals kept together leads to crosscontamination by oral bacteria
CARIES
Figure 2:Diagram summarising results of studies on caries and bacteria
![Page 12: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/12.jpg)
![Page 13: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/13.jpg)
New born (2 days)
98% facultative streptococci , lactobacilli
Few anaerobic organisms
Few G (+),yeasts
![Page 14: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/14.jpg)
Vaginal lactobacilliAir – borne bacteriaOther microorganismsFood、Water、Air dust、Dirt、Parents、Medical personnel
![Page 15: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/15.jpg)
![Page 16: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/16.jpg)
TABLE 2. CHANGES IN THE ORAL FLORA ASSOCIALTED WITH THE ERUPTION OF TEETH
Organism Before Eruption After EruptionSreptococcus salivarius usually present usually presentSreptococcus sanguis usually absent usually presentSreptococcus mutans usually absent usually presentActinomyces sp. usually absent usually presentBacteroides sp. usually absent usually presentFusobacterium sp. usually absent usually presentLactobacillus sp. usually present usually present
(low numbers)Veillonella sp. usually present usually present
![Page 17: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/17.jpg)
Initial reports of mutans sterptococciClark (1924)
G(+),chain – forming cells
![Page 18: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/18.jpg)
Fitzgerald and Keyes (1968) Mutans Streptococci began to
appear in literature
![Page 19: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/19.jpg)
S.Mutans are the main bacteria cause of caries
(Emission and Krasse 1985)(Loesche 1986)
![Page 20: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/20.jpg)
![Page 21: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/21.jpg)
Physiology of cariogenic bacteria
![Page 22: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/22.jpg)
Extracellular and cell – surface polymers synthesis
Glucans (glucose homopolymers) –dextran
Fructans (fructose homopolymers) -levan
![Page 23: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/23.jpg)
Sucrose GTF GlucanG-O-F (G-O-G)n+1 + Fructose
Glucosyl transferase
Sucrose FTF FructanG-O-F (F-O-F)n+1 + Glucose
Fructosyl transferase
![Page 24: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/24.jpg)
Sucrose GTF glucose + fructose
Glucose GTF-S water-soluble glucans
Glucose GTF-I water-insoluble glucans
![Page 25: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/25.jpg)
Glucans
Water – solubleplaque
Alkali – soluble
![Page 26: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/26.jpg)
Fructans
Water – soluble plaque
![Page 27: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/27.jpg)
Degradation of glucans & fructans
H2O + Fructan Fructanase nFructose
H2O + Glucans Dextranase nGlucose
![Page 28: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/28.jpg)
Intercellular metabolism and polymer formation
(Lactic acidogenic theory)
![Page 29: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/29.jpg)
The synthesis of intercellular polysaccharide (IPSs) is not sucrose –
dependant any fermentable carbohydrate can serve
![Page 30: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/30.jpg)
Extracellular polysaccharides glucans, fructans
Intracellular polysaccharides glycogen
![Page 31: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/31.jpg)
Glycolysis
Acid production
IPS synthesis
![Page 32: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/32.jpg)
![Page 33: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/33.jpg)
Virulence (Cariogenicity)determinants of the mutans streptocci
Water-insoluble glucan synthesisIntercellular polysaccharide synthesisAcid toleranceLactic acidogenicityEndo dextronase
![Page 34: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/34.jpg)
Molecular pathogenesis
Initial attachment
Accumulation
Acid formation and cavitation
![Page 35: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/35.jpg)
Molecular pathogenesis
Initial attachment pellicle binding protein
![Page 36: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/36.jpg)
Specifical binds glucan(glucan binding protein)
![Page 37: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/37.jpg)
![Page 38: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/38.jpg)
Accumulation
Glucosyltransferase (GTF)→glucansGTF-S (water-soluble glucans)GTF-I (water-insoluble glucans)Glucan binding protein (a mutans receptor)
![Page 39: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/39.jpg)
![Page 40: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/40.jpg)
Acid formation and cavitation
Lactic acid
![Page 41: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/41.jpg)
![Page 42: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/42.jpg)
Cariogenic features of mutans streptococci
1. Binding to and colonization of teeth2. Accumulation on tooth surfaces and participation
in the formation of dental plaques.3. Production of acid at high rate.4. Tolerance of high concentrations of sugar, high
ionic strength, and highly acidic conditions.
![Page 43: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/43.jpg)
Cariogenic features of mutans streptococci
5. Association with dental caries in humans.6. Causation of dental caries in animals.7. Transmissible in animals and apparently in man.8. Reduction or elimination of mutans streptococci
will result in reduction or elimination of dental caries.
![Page 44: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/44.jpg)
Monitoring the cariogenic flora in clinical practice and research
Sampling
Transporting
Diluting
Plating
![Page 45: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/45.jpg)
![Page 46: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/46.jpg)
Oral streptococci
S. sanguisS. mutansS. salivariusS. mitis
![Page 47: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/47.jpg)
S. mutans
Mitis salivarus agar (MS)
MSB (20% sucrose + 0.2 u/ml bacitracin)
G(+) , cushion – shaped , frosted glass
![Page 48: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/48.jpg)
![Page 49: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/49.jpg)
![Page 50: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/50.jpg)
![Page 51: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/51.jpg)
Taxonomy of mutans sterptococii
Species Bratthall / perch serotype
S. cricetus aS. rattus bS. mutans c.e.f.S. sobrinus d.g.SL
![Page 52: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/52.jpg)
![Page 53: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/53.jpg)
TABLE 1 Summary of Serotyping Results of S mutans
Serotype Total Number %a 0 0b 1 .4c 184 79.7d 0 0e 22 9.5f 13 5.6g 1 .4Nontypeable 10 4.4
231 100.0
![Page 54: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/54.jpg)
Oral lactobacilli
Bunting (1925) Bacillus acidophilus
G(+), non – spore forming rods
Rogosa agar
![Page 55: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/55.jpg)
Homofermentative Heterofermentative
L. casei L. fermentumL. acidophilus L. brevisL. plantarum L. buchneriL. salivarius L. cellobiosus
![Page 56: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/56.jpg)
Oral actinomyces
G(+).non – spore forming rod and filaments (NAC – 20 ,CFAT)
![Page 57: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/57.jpg)
Anaerobic Facultative Anaerobic
A. bovis A. viscosus
A. israelii A. naeslundii
A. odontolyticus
![Page 58: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/58.jpg)
![Page 59: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/59.jpg)
Immunological aspects of dental caries
![Page 60: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/60.jpg)
Immunological protection
IntravenousIntraductalSubcutaneousSubmucosal
![Page 61: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/61.jpg)
The immune response to streptococcal antigens
Thymus-derived T cell
Bone-marrow derived B cell
![Page 62: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/62.jpg)
![Page 63: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/63.jpg)
COMPONENTS OF ORAL IMMUNITY
Salivarygland
Salivarysecretion
Blood crevicular fluid
IgG, IgM , IgAProteinsComplementEnzymesElectrolytesPolymorphsB lymphocytesT lymphocytesMacrophagesSecretory IgAProteinsEnzymesElectrolytes
Secretory IgAIgG, IgAProteinsEnzymesElectrolytesPolymorphs
Whole saliva
![Page 64: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/64.jpg)
Serum antibody and disease
Caries-free:greater serum antibody inhibits GTF
Caries-prone:less serum antibody
![Page 65: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/65.jpg)
Effect of antibody to glucosyltransferase
GTF-I
GTF-S
![Page 66: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/66.jpg)
Dental caries and the mucosal immune system
Salivary antibody (IgA)
Gingival crevicular (IgG)
![Page 67: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/67.jpg)
Individuals with immunologic deficiency
IgA deficiency --- dental caries elevated
IgM compensatory --- less dental caries
![Page 68: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/68.jpg)
Role of antibody in dental caries
IgA antibody makes up less than 1% of the total IgA
![Page 69: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/69.jpg)
S. mutans (serotype c,e,and f)
S. sobrinus (serotype d,g,and h)
![Page 70: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/70.jpg)
Salivary antibody and disease
Salivary antibody level depends on the rate of salivary flow
![Page 71: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/71.jpg)
![Page 72: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/72.jpg)
Sugar substitutes and caries
SorbitolMannitolXylitolPenitol
![Page 73: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/73.jpg)
Saliva
The function of saliva are:1. Lubrication2. Cleansing3. Digestion4. Antiacid5. Antibacterial
![Page 74: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/74.jpg)
Fluoride effect on cariogenic bacteria
![Page 75: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/75.jpg)
Conclusion
It is clear that caries is a complex disease whose etiology involves multiple interactions at the molecular level and thus whose prevention will require an understanding of the entire process at this level.
![Page 76: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/76.jpg)
References
1. Slots. Taubman: Contemporary oral microbiology and immunology,1990,377-543
2. Ernest Newbrum: Cariology,1989.3rd edi;Quintessence publishing Co. Inc, Chicago. Chapter 2~4,5,10~12
3. Brian H. Clarkson : Introduction to Cariology Dent.Clin North Amer43:569~578, 1999
![Page 77: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/77.jpg)
Curriculum Vitae
Name: Hong -Sen ChenSex: MaleData and Place of Birth: August,25,1949. MiaoliCounty, TaiwanMarital Status: MarriedEducation:
1. Undergraduate College: Kaohsiung Medical College B.D.S. 1969~1975
2. Graduate SchoolsKaohsiung Medical College M.S. 1985~1988Kaohsiung Medical University Ph.D 1990~1996
![Page 78: Microbiological & Immunological Aspects of Dental cariesgceohrd.so-buy.com/ezfiles/gceohrd/img/img/139055/... · ETIOLOGICAL FACTORS IN DENTAL CARIES Caries No Caries No Caries No](https://reader030.fdocuments.net/reader030/viewer/2022040121/5ea26231550e3a1ce71a3090/html5/thumbnails/78.jpg)
Academic Appointments1. Lecture: school of dentistry,1983~1995
Kaohsiung Medical College2. Associate Professor. School of Dentistry and
Faculty of Dental Hygiene 1996~to date. Kaohsiung Medical University