Micro Total Analysis Systems 2003Volume 2

Proceedings of uTAS 2003Seventh International Conference on Miniaturized Chemicaland Biochemical Analysis Systemsheld in Squaw Valley, California

USAOctober 5-9, 2003

edited by

M. Allen Northrup,MicroFluidic Systems Inc., USA

Klavs F. Jensen,Massachusetts Institute of Technology, USA

and

D. Jed HarrisonUniversity of Alberta, CANADA

Sponsored byTransducers Research Foundation

with Technical Support fromElectron Devices Society

ISBN: 0-9743611-0-0TRF Catalog Number: 03TRF-0001

TECHNISCHEINFORMATIONSBIBLIOTHEK

UNIVERSITAT8BIBLIOTHEKHANNOVER

Table of ContentsVolume 2

Day 3 - Wednesday, October 8,2003

Plenary VDNA MANIPULATION IN ELECTROSTATIC FIELDS 869Masao WashizuUniversity of Tokyo

Concurrent Session - Fluidics IA NOVEL MICROINJECTION SYSTEM BY FUSING GLASS NEEDLEAND PDMS-BASED VALVE WITH RIGID CENTER MEMBRANE 875S.H. Lee' and DJ. Beebe2

'Dankook University and' University of Wisconsin

PARTICLE FLOW SWITCH UTILIZING ULTRASONIC PARTICLE SWITCHINGIN MICROFLUIDIC CHANNELS 879F. Petersson, A. Nilsson, H. Jonsson and T. LaurellLund Institute of Technology

AN OSCILLATING FERROMAGNETIC MICROPUMP UTILIZINGCENTRIFUGAL FORCE 883J. Atencia and DJ. BeebeUniversity of Wisconsin

Concurrent Session - Proteomics IIIA CAPILLARY FILLING MICROSYSTEM FOR SOLID-PHASE EXTRACTIONAND DISPENSING OF PROTEOMICS SAMPLES 887L. Wallman', S. Ekstrom', J. Nilsson', G. Marko-Varga2 and T. Laurell''Lund Institute of Technology and AstraZeneca Research & Development

MICROSECOND MICROFLUIDIC MIXING FOR INVESTIGATION OF PROTEINFOLDING KINETICS 891D.E. Hertzog1'2, J.G. Santiago1 and O. Bakajin2

'Stanford University and 2Lawrence Livermore National Laboratory

COLORIMETRIC ANALYSIS CHIP CHECKING HEPATIC FUNCTIONS 895A. Yokogawa', A. Oki', T Shimasaki1, H. Takasu1 and Y. Horiike2

'Rohm Co., Ltd. and2National Institute for Materials Science

XXXV

Concurrent Session - Fluidics IIFIELD EFFECT FLOW CONTROL IN A POLYMER T-INTERSECTIONMICROFLUIDIC NETWORK 899N.J. Sniadecki', R. Chang', M. Beamesderfer', C.S. Lee' andD.L. DeVoeUniversity of Maryland and 'NASA Goddard

A FLOW SWITCH BASED ON CORIOLIS FORCE 903T. Brenner, T. Glatzel, R. Zengerle andJ. DucreeUniversity of Freiburg

ON-DEVICE DILUTION TO FIVE LOGS WITH INCREASED THROUGHPUT ONA LabCD™: APPLICATION TO INHIBITION ASSAYS 907G.J. Kellogg and T.J. JohnsonTecan Boston

Concurrent Session - Cells IIIA MICROELECTRONIC CHIP OPENS NEW FIELDS IN RARE CELLPOPULATION ANALYSIS & INDIVIDUAL CELL BIOLOGY 911A. Fuchs', N. Manaresi2, D. Freida'', L. Altomare3, C.L. Villiers1, G. Medoro2, A. Romani,I. Chartier', C. Bory', M. Tartagni3, P.N. Marche', F. Chatelain1 and R. Guerrieri3

'CEA, 'Silicon Biosystems and3 University of Bologna

MICRO ISOELECTRIC FREE FLOW SEPARATION OFSUBCELLULAR MATERIALS 915H. Lu, S. Gaudet, P.K. Sorger, M.A. Schmidt andK.F. JensenMassachusetts Institute of Technology

PROGRAMMABLE DIELECTROPHORETIC pTAS SAMPLE HANDLING 919P.R.C. Gascoyne', J. V. Vykoukal', T. Anderson'', J. Noshari', F.F. Becker'',K. Ratanachoo2, K. Kandjanapa2, J. Satayavivad2 and M. Ruchirawat2

'University of Texas M.D. Anderson Cancer Center and 2Chulabhorn Research Institute

Poster Sessions - MicrofluidicsBIOSENSOR PERFORMANCE ENHANCEMENT THROUGHAC ELECTROKINETICS 923M. Sigurdson, CD. Meinhart, X. Liu andD. WangUniversity of California, Santa Barbara

MICRO OPTICAL SWITCHING VALVE SYSTEM BASED ON REVERSIBLEWETTABILITY CONVERSION 927H. Nagai1, J. Takahashi2 and S. Wakida''National Institute of Advanced Industrial Science and Technology and'Daikin Environmental Laboratory, Ltd.

XXXVI

HIGH-YIELD BURIED MICROCHANNEL FORMATION FOR DRUG DELIVERYAT THE CELLULAR LEVEL 931Y. Li, N. Gulari and K.D. WiseUniversity of Michigan

MIXING AND FILTERING IN A CROSS-CHANNEL INTERSECTION 935A. Dodge', M.-C. Jullien2, F. Okkels' and P. Tabeling''ESPCI and 2ENS-Cachan

ACOUSTIC STREAMING - ULTRASONIC AGITATION IN MICROCHANNELS 939M. Bengtsson and T. LaurellLund Institute of Technology

MEASUREMENT OF FAST KINETICS IN A MICROFLUIDIC SYSTEM 943C. Baroud', L. Menetrier2, F. Okkels2 and P. Tabeling1

'Ecole Polytechnique and 2ESPCI

SURFACE MOUNT MICROFLUIDIC FLOW REGULATOR ON APOLYMER SUBSTRATE 947M.J. Mescher, C.E. Dube, M. Varghese and J.O. FieringCharles Stark Draper Laboratory

THE EFFECT OF SURFACE CHARACTER ON FLOWS INCYLINDRICAL MICROCHANNELS 951L.E. Rodd\ G. Rosengarten\ S.T. Huntington1, K. Lyytikainen2, D.V. Boger'andJ.J. Cooper-White1

'University of Melbourne and 2 University of Sydney

ON-CHIP IEF PEAK MANIPULATION FOR 2D PROTEINSEPARATION AND MS COUPLING 955Y.-C. Wang, M.H. Choi andj. HanMassachusetts Institute of Technology

SOFT EMBOSSING OF MICROFLUIDIC DEVICES 959B.L. Carvalho, E.A. Schilling, N. Schmid and G .J. KelloggTecan Boston

NANOJET CONTROLLED DROPLET EMULSION IN MICROFLUIDICCHANNELS 963Y.-C. Tan, J. Collins and A. P. LeeUniversity of California, Irvine

T-SENSOR GENERATED REFRACTIVE INDEX GRADIENTS:CALIBRATION OF AN SPR MICROSCOPE 967J. Folev, E. Fu and P. YagerUniversity of Washington

XXXVII

Poster Sessions - Microfluidics, continuedULTRAFAST ON-CHIP SEPARATIONS OF CYTOKINES BY SDS-PAGEUSING UV-INITIATED POLYACRYLAMIDE 971R. Shediac, S.A. Pizarro, A.E. Herr and A.K. SinghSandia National Laboratories

A NOVEL MICRODISPENSER ARRAY FOR ACCURATE OFF-CHIP DISPENSINGFOR MICROARRAY APPLICATIONS 975A. Puntambekar and C.H. AhnUniversity of Cincinnati

HOMOGENEOUS IMMUNOASSAY FOR DETECTION OF TNT AND ITSANALOGUES ON A MICROFABRICATED CAPILLARY ARRAYELECTROPHORESIS CHIP 979A. Bromberg and R.A. MathiesUniversity of California, Berkeley

ELECTROKINETIC MICROFLOW INSTABILITY WITHCONDUCTIVITY GRADIENTS 983C.-H. Chen, H. Lin, S.K. Lele andJ.G. SantiagoStanford University

OPTIMIZATION OF ISOTACHOPHORESIS COUPLED WITH GELELECTROPHORESIS IN MICROFLUIDIC DEVICES FOR HIGH THROUGHPUTSCREENING APPLICATIONS 989C. Park, P. Kechagia, R.-L. Chien andM. SpaidCaliper Technologies Corp.

AUTOMATED ELECTROWETTING BASED DROPLET DISPENSING WITHGOOD REPRODUCIBILITY 993H. Ren, V. Srinivasan, M. Pollack and R.B. FairDuke University

CHARACTERIZATION OF PIEZOELECTRIC MICROPUMP DRIVENBY TRAVELING WAVES 997/. Kanno', S. Kawano2, S. Yakushiji' and H. Kotera''Kyoto University and 2Tohoku University

A THREE-DIMENSIONAL MICROFLUIDIC NETWORK FORCELLULAR PERFUSION 1001Y. Choi, R. Powers, Y.-K. Yoon and M.G. AllenGeorgia Institute of Technology

REDUCTION OF MICROFLUIDIC END EFFECTS IN MICRO-FIELDFLOW FRACTIONATION CHANNELS 1005J. W. Kim, H.J. Sant andB.K. GaleUniversity of Utah

PASSIVE MIXING AND THE FLOW CHARACTERISTIC OF AH-TYPE MICROCHANNEL 1009ID. Yang, Y.F. Chen, H.T. Hsu, F.G. Tseng and C.C. ChiengNational Tsing Hua University

FLUIDICS RESOLVED ANALYSIS OF PROTEIN ADSORPTIONIN A BIOMICROFLUIDIC SYSTEM 1013J. Jenkins', B. Prabhakarpandian', K. Lenghaus2, J. Hickman2 and S. Sundaram'CFD Research Corporation and'Clemson University

A NOVEL BIOPARTICLE SEPARATION TECHNIQUE USING SEQUENTIALPRESSURE PULSES 1017A. Jain, A. Puntambekar and C.H. AhnUniversity of Cincinnati

RP-HPLC MICROCHIP SEPARATIONS WITH SUBNANOLITER ON-CHIPPRESSURE INJECTIONS 1021D.S. Reichmuth, T.J. Shepodd andB.J. KirbySandia National Laboratories

SURFACE MICROMACHINED HOLLOW MICRONEEDLE ARRAY INTEGRATEDON A MICROFLUIDIC CHIP 1025T. Ichiki'2, Y. Sugiyama , S. Kase2 and Y. Horiike3

'Toyo University, Japan Science and Technology Corporation and3 National Institute for Materials Science

LIQUID SLUG TRANSPORT, SYNCHRONIZATION AND MIXINGDRIVEN BY FERROFLUIDS 1029O. Sorensen, K.S. Drese andS. HardtInstitut fur Mikrotechnik Mainz GmbH

DEVELOPMENT OF MICRO NEEDLE-HEAD SLIDE VALVE UNITFOR MICROFLUIDIC DEVICES 1033K. Morishima , M. Ohnishi , M. Tokeshi and T. Kitamori ""Kanagawa Academy of Science and Technology, ' University of Tokyo andJapan Science and Technology Corporation

Poster Session - MEMS TechnologyPATCH-CLAMP ARRAY WITH ON-CHIP ELECTRONICS, OPTICS,FLOW CONTROL AND MECHANICAL ACTUATION 1037M. Okandan, C. James, S.S. Mani andB.L. DraperSandia National Laboratories

CHEMISTRY AT LIQUID-LIQUID INTERFACES 1041N.O.L. Viernes and J.S. MooreUniversity of Illinois, Urbana-Champaign

MAGF: A NEW METHOD FOR HIGH-PERFORMANCE FOCUSING OFUNCHARGED AND HYDROPHOBIC ANALYTES 1045D. Ross, KM. Balss, P.B. Howell and W.N. VreelandNational Institute of Standards and Technology

A RECONFIGURABLE INTEGRATED DEVICE FOR BIOASSAYDEVELOPMENT 1049G.A. Mensing, D. Kim, J. Moorthy, J.I. Bassett, D.T. Eddington andD.J. BeebeUniversity of Wisconsin

FABRICATION OF THROUGH-WAFER FLUID INTERCONNECTS WITH LOWDEAD VOLUME AND INTEGRATED BACK-PLANE FLUID JUMPERS 1053K. Shaikh, K.S. Ryu, Z. Fan and C. LiuUniversity of Illinois, Urbana-Champaign

USE OF CONDUCTIVITY ELECTRODES AS ON-CHIP SENSORS INCHROMATOGRAPHIC MICROFLUIDIC DEVICES 1057R. Rocklin', H. Yin', D. Sobek', P. Coufal2 andK. Killeen''Agilent Technologies Inc. and 2Charles University

A NOVEL SYSTEM FOR MEASUREMENT OF ELECTROPHYSIOLOGICALSIGNALS ASSOCIATED WITH TRAUMATIC NEURONAL INJURY 1061J. Ross, G. Prado, Y. Choi, M. Allen, M. LaPlaca andS. DeWeerthGeorgia Institute of Technology

MICRO-PHYSIOMETRY TOOLS FOR CELL ANALYSIS ANDCELL SEPARATIONS 1065R. Pethig, M.S. Talary, R.S. Lee, B. Kusler and C. Carswell-CrumptonAura BioSystems, Inc.

ON THE IMPORTANCE OF QUALITY CONTROL IN MICROFLUIDICDEVICE MANUFACTURING 1069M.R. Steedman, KM. Lloyd, A. Hatch, M.S. Munson and P. YagerUniversity of Washington

ACTIVE LAB-ON-CHIP WITH A DISPOSABLE FLUIDIC SYSTEM 1073R.A. BuserUniversity of Applied Science Buchs

Poster Session - MaterialsPRECONCENTRATION OF PROTEINS ON CHIP USING A PHOTOINITIATEDPOLYMER MONOLITH CONTAINING IMMOBILIZED TANNIC ACID 1077L.M. Barrett12, F. Svec3 and Y. Fintschenko2

1Loughborough University, 2Sandia National Laboratories and3University of California, Berkeley

NOVEL SURFACE MODIFICATION METHODS AND SURFACE PROPERTYANALYSIS FOR SEPARATION OF DNA BIO-MOLECULES USINGCAPILLARY ELECTROPHORESIS 1081C.-H. Lin', L.-M. Fu2, K.-H. Lee3, R.-J. Yang3 andG.-B. Lee3

'National Sun Yat-Sen University, 2National Pingtun University of Science and Technologyand 3National Cheng Kung University

SURFACE MODIFICATION OF SiO2 MICRONOZZLES FOR PATCH-CLAMPMEASUREMENTS ON-CHIP 1085T. Lehnert, A. Laine, M.A.M. GijsSwiss Federal Institute of Technology Lausanne

DEVELOPMENT OF PROCESS PROTOCOLS TO FINE TUNEPOLYDIMETHYLSILOXANE MATERIAL PROPERTIES 1089D.T. Eddington, W.C. Crone and DJ. BeebeUniversity of Wisconsin

SURFACE CHARACTERIZATION AND A LIFT-OFF PROCESS OF AFLUOROCARBON THIN FILM FOR MICRO PROTEIN PATTERNING 1093S.-H. Lee, C.-S. Lee, B.-G. Kim and Y.-K. KimSeoul National University

POROUS SILICON AS SUBSTRATE FOR HIGH SENSITIVITY PROTEIN CHIPS .... 1097A. Ressine', G. Marko- Varga2 and T. Laurell1

1 Lund Institute of Technology and2Lund University

PROTEIN MICROARRAY ON CYCLIC OLEFIN COPOLYMER (COC)FOR DISPOSABLE PROTEIN LAB-ON-A-CHIP 1101J. Kai, Y.S. Sohn and C.H. AhnUniversity of Cincinnati

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Poster Session - Materials, continuedPOLY (ETHYLENE GLYCOL) (PEG)-MODIFIED POLY (DIMETHYLSILOXANE)(PDMS) FOR PROTEIN- AND CELL-RESISTANT SURFACES INMICROBIOREACTOR 1105H.-G. Choi', Z. Zhang1, P. Boccazzi', P.E. Laibinis2, AJ. Sinskey' andK.F. Jensen''Massachusetts Institute of Technology and2Rice University

PARYLENE TECHNOLOGY FOR MECHANICALLY ROBUST NEURO-CAGES 1109E. Meng, Y.-C. Tai, J. Erickson andJ. PineCalifornia Institute of Technology

UV ADHESIVE BONDING TECHNIQUES AT ROOM TEMPERATUREFOR PLASTIC LAB-ON-A-CHIPS 1113J. Han, S. Lee, A. Puntambekar, S. Murugesan, J.-W. Choi, G. Beaucage and C.H. AhnUniversity of Cincinnati

SU-8 HYDROPHILIC MODIFICATION BY FORMING COPOLYMERWITH HYDROPHILIC EPOXY MOLECULE 1117C.-L. Wu, M.-H. Chen andF.-G. TsengNational Tsing Hua University

IMPROVEMENT OF ELECTROOSMOTIC FLOW CHARACTERISTIC INPOLY(DIMETHYLSILOXANE) CHANNELS VIA A LONG LIFE CHEMICALSURFACE MODIFICATION 1121E.S. Choi and S.S. YangAjou University

BIO ACTUATED MICROSYSTEM USING CULTURED CARDIOMYOCYTES 1125K. Morishima', Y. Tanaka2, K. Sato23, M. Ebara", T. Shimizu5, M. Yamato5, A. Kikuchi5,T. Okano5andT. Kitamori123

'Kanagawa Academy of Science and Technology, 2 University of Tokyo, 3Japan Science andTechnology Corporation, 4Waseda University and5 Tokyo Women's Medical University

AUTOMATIC TRANSPORTATION OF A DROPLET ON A WETTABILITYGRADIENT SURFACE 1129T. Yasuda1, K. Suzuki2 and I. Shimoyama2

'Kyushu Institute of Technology and 2University of Tokyo

Poster Session - ApplicationsCONVECTIVELY DRIVEN POLYMERASE CHAIN REACTIONTHERMAL CYCLER 1133E.K. Wheeler, B. Benett, P. Stratton, J. Richards, A. Christian, A. Chen, T. Weisgraber,K. Ness, J. Ortega andF. MilanovichLawrence Livermore National Laboratory

xlii

REMOVAL OF PCR INHIBITORS USING DIELECTROPHORESIS FOR SAMPLEPREPARATION IN A MICROFABRICATED SYSTEM 1137A. Wolff, I.R. Perch-Nielsen', C.R. Poulsen', J. El-Ali' and D.D. Bang2

'Technical University of Denmark and2Danish Veterinary Institute

TEMPERATURE GRADIENT FOCUSING OF MATCHED AND PARTIALLYMISMATCHED DNA/PNA HYBRIDIZATIONS 1141KM. Balss, D. Ross and MJ. TarlovNational Institute of Standards and Technology

DIELECTROPHORETIC FOCUSING AND SEPARATION OF NANOPARTICLES:EXPERIMENTAL MEASUREMENT AND NUMERICAL SIMULATION 1145N.G. Green, D. Holmes and H. MorganUniversity of Glasgow

IMAGING FOR DEGRADATION OF IKB-EGFP IN A SINGLE JURKATT CELL STUDIED WITHIN A MICROFLUIDIC CHANNEL 1149P.C.H. Li, L. de Camprieu and M. SangarSimon Fraser University

DIELECTROPHORESIS BASED MICRO FLOW CYTOMETERY 1153J. Vykoukal', J.A. Schwartz', P.R.C. Gascoyne', C. Yu2 andL. Shi2

'University of Texas M.D. Anderson Cancer Center and2 University of Texas, Austin

PROTOTYPE OF A MULTIPLE REACTIONS DNA ANALYSIS DEVICEWITH A VERSATILE MICROFLUIDIC SAMPLE MANIPULATOR 1157M.-B. Yim, R. Lin, M. Yang, B.N. Johnson, D.T. Burke andM.A. BurnsUniversity of Michigan

GRADIENT-ELUTION REVERSED-PHASE ELECTROCHROMATOGRAPHYIN MICROCHIPS 1163A.K. Singh, DJ. Throckmorton, J.S. Brennan and TJ. ShepoddSandia National Laboratories

MONITORING OF SUBCELLULAR FUNCTIONS ON MICROCHIP 1167J. Dragoljic and DJ. HarrisonUniversity of Alberta

ELECTROKINETIC PRECONCENTRATION OF PROTEINS ON THINPDMS MEMBRANES 1171S.M. Kim and E.F. Hasselbrink, Jr.University of Michigan

xliii

Poster Session - Applications, continuedPROTEASE DIFFUSION AND REACTION ON IMMOBILIZEDSUBSTRATE SURFACES 1175S. Roy', J.T. Kellis1, A.J. Polouse2, C.R. Robertson' andA.P. Gast3

Stanford University, 2Genencor International Inc. and3Massachusetts Institute of Technology

CHIP-BASED RISK EVALUATION SYSTEM FOR CHEMICALS USING E.COLI 1179M. Tokeshi', S. Hiki2, Y. Akiyama2, K. Morishima1, K. Sato1 and T. Kitamori13

Kanagawa Academy of Science and Technology, Institute of Microchemical Technology and3 University of Tokyo

SIZE DEPENDENT MOBILITY OF DNA IN ELECTRIC AND HYDRO DRAGFORCE FIELD 1183Y. Takamura', Y. Horiike2, Y. Baba3 andE. Tamiya''japan Advanced Institute of Science and Technology,2 National Institute of Materials Science and 3 University ofTokushima

ENHANCED BROWNIAN RATCHET ARRAY FOR DNA SEPARATION USINGFLOW ANGLE EFFECT 1187L.R. Huang, J.C. Sturm, R.H. Austin andE.C. CoxPrinceton University

HIGH THROUGHPUT SEPARATIONS USING A MICROFABRICATED SERIALELECTRIC SPLITT SYSTEM 1191S. Merugu, N. Narayanan and B.K GaleUniversity of Utah

A MICRO-GAP SENSOR BASED ON CONDUCTING POLYPYRROLE 1195K.-S. Teh andL. LinUniversity of California, Berkeley

MICROFABRICATED CYCLICAL ELECTRICAL FIELD FLOWFRACTIONATION 1199A.S. Kantak, S. Merugu and B.K. GaleUniversity of Utah

RAPID GENOTYPING WITH INTEGRATED CONTINUOUS-FLOW PCR ANDBIOELECTRONIC DETECTION 1203C.-F. Chou, R. Lenigk', D. Sadler2, R. Changrani3, S. O'Rourke', R. Liu' andF. Zenhausern''Arizona State University, ' Motorola Labs and3Northwestern University

SEPARATION OF BIOPARTICULATE MATTER USING TRAVELING WAVEDIELECTROPHORESIS 1207K. Pant, J. Feng, G. Wang, S. Krishnamoorthy and S. SundaramCFD Research Corporation

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2 1/2 D MICROFABRICATED NIB-LIKE SOURCES FOR NANOELECTROSPRAYAPPLICATIONS 1211S. Le Gac, S. Arscott and C. RolandoUMR CNRS

ANALYSIS OF MOLECULAR BINDING TO HUMAN SERUM ALBUMINUSING A T-SENSOR 1215A. Hatch, E. Garcia and P. YagerUniversity of Washington

Poster Session - Detection TechnologiesFIRST MEASUREMENT RESULTS WITH AN INTEGRATED PROJECTIONCYTOMETER 1219J.H. Nieuwenhuis', J. Bastemeijer2, P.M. Sarro2 and MJ. Vellekoop''Vienna University of Technology and2 Delft University of Technology

A NOVEL HIGH-FREQUENCY SENSOR FOR BIOLOGICAL DISCRIMINATION.... 1223T.C. Messina1, L.N. Dunkleberger', G.A. Mensing2, A.S. Kalmbach3, R. Weiss3, DJ. Beebe2

and L.L. Sohn'University of California, Berkeley, 'University of Wisconsin and Princeton University

MICROSCALE IMPEDANCE-BASED DETECTION OF BACTERIALMETABOLISM 1227R. Gomez, D. Akin, A.K. Bhunia, M.R. Ladisch andR. BashirPurdue University

INTEGRATED MICROFLUIDIC GAS SENSORS FOR WATER MONITORING 1231L. Zhu', C. Kimball', N. Sniadecki', M. Beamesderfer2, S. Semancik3, andD.L. DeVoe'University of Maryland, 2NASA Goddard Space Flight Center and3National Institute of Standards and Technology

TOWARDS A FULLY AUTOMATED PLANAR PATCH-CLAMP BASED DOSE-RESPONSE MEASUREMENT SYSTEM 1235B. Matthews andj. W. JudyUniversity of California, Los Angeles

IMPEDANCE BASED SINGLE CELL DETECTION USING RESONANCEFREQUENCY INTERROGATION 1239D. Holmes and H. MorganUniversity of Glasgow

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Poster Session - Detection Technologies, continuedPASSIVE CALIBRATION-VAPOR SOURCE FOR A MICRO GASCHROMATOGRAPH 1243M.C. Oborny'2, J. Zheng13, J.M. Nichols', C.-J. Lu', P.L. Bergstrom'3, R.P. Manginell2,G.C. Frye-Mason24 andE.T. Zellers''University of Michigan, 2Sandia National Laboratories,3Michigan Technological University and 4Nomadics Inc.

MONOLITHICALLY-INTEGRATED MICROCHEMLAB FOR GAS-PHASECHEMICAL ANALYSIS 1247R.P. Manginell, M. Okandan, RJ. Kottenstette, P.R. Lewis, D.R. Adkins, J.M. Bauer,R.G. Manley, S. Sokolowski and RJ. ShulSandia National Laboratories

DYE-FLUORESCENCE LED-SPEC: A BATTERY-OPERATED, ON-CHIP,WAVELENGTH-TUNABLE OPTICAL SOURCE FOR DETECTION OFBIOCHEMICALS 1251L. Que, P. Selvaganapathy, B. Mitra, C.G. Wilson and Y.B. GianchandaniUniversity of Michigan

MICRO CONFOCAL LINE SCANNING SYSTEM FORMULTIPLEXED BIOCHIPS 1255S. Kwon, G.L. Liu, K.-H. JeongandL.P. LeeUniversity of California, Berkeley

AN INTEGRATED OPTICAL DETECTOR FOR MICROFABRICATEDELECTRICAL FIELD FLOW FRACTIONATION SYSTEM 1259HJ. Sant and B.K. GaleUniversity of Utah

MICROSPECTROMETER USING OPTICAL DIVERSITY 1263W.-C. Shih, C. Hidrovo, S.-G. Kim and G. BarbastathisMassachusetts Institute of Technology

PROTEIN MASS DETECTION SYSTEM IN LIQUID FOR PROSTATE-SPECIFICANTIGEN (PSA) USING PDMS LIQUID CELL BY RESONANT FREQUENCYSHIFT OF MICROMACHINED PZT CANTILEVER 1267K.S. Hwang12, J.H. Lee', J. Park1, J.H. Park2 and T.S. Kim''Korea Institute of Science and Technology and'Korea University

xlvi

Concurrent Session - Droplet ProcessingSEPARATION, TRAPPING, AND ANALYSIS OF BIOLOGICALNANO-PARTICLES IN BIOMEMS 1271M. Stelzle', M. Diirr', G. Gradl3, P. Geggier2, A. Haage4, R. Hagedorn5, M. Jdger2,J. Kentsch , T. Mutter , A. Normann and T. SchnelleNatural and Medical Sciences Institute, 'Fraunhofer Institute for Biomedical Engineering,Evotec Technologies GmbH, Mediagnost GmbH and Humboldt University, Berlin

IN-PLANE DIGITAL SORTING OF FLOW-THROUGH DROPLET PHASEMICROFLUIDS 1275J.S. Go', E.H. Jeong2, K.C. Kim3, S.Y. Yoon3 andS. Shoji'1 Waseda University, 'Japan Science and Technology Corporation and' Pusan National University

COMBINING DROPLET-BASED LIQUID HANDLING AND ON-CHIP CAPILLARYELECTROPHORESIS WITH A NEW SAMPLE INJECTION METHOD 1279S. Kaneda and T. FujiiUniversity of Tokyo

Concurrent Session - ApplicationsGENETIC ANALYSIS USING A PORTABLE PCR-CE MICROSYSTEM 1283E.T. Lagally, J.R. Scherer, R.G. Blazej andR.A. MathiesUniversity of California, Berkeley

CLINICAL DIAGNOSTICS ON HUMAN WHOLE BLOOD, PLASMA,SERUM, URINE, SALIVA, SWEAT, AND TEARS ON A DIGITALMICROFLUIDIC PLATFORM 1287V. Srinivasan, V.K Pamula, M.G. Pollack and R.B. Fan-Duke University

SMART DISPOSABLE PLASTIC LAB-ON-A-CHIP FOR POINT-OF-CARE-TEST1NG (POCT) 1291A. Puntambekar', C. Hong', C. Gao', X. Zlm', R. Trichur'. ./. Han', S. Lee1, J. Kai'. J. Do1.R. Rong', S. Chihikuru', M. Dutta', L. Ramasamy', S. Mtirugesan', R. Cole1, J. Nevin1.G. Beaucage', J.B. Lee2, J. Y. Lee3, M. Bissell3. J. W. Choi' and C.H. Aim1

'University of Cincinnati, 'University of Texas and 'Ohio State University

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Day 4 - Thursday, October 9, 2003

Plenary VIFABRICATION OF A n-SCALE HIGH THROUGHPUT PLATFORM FOR HIGHDENSITY GENE EXPRESSION AND GENOTYPING APPLICATIONS 1295Martin J. Goldberg, H. Dong, D. Nguyen, M. Shirazi, M. Yamamoto, Q. Yang and T. YuAffymetrix Inc.

Concurrent Session - Optics INOVEL SPECTROSCOPIC TOOL FOR MOLECULAR BEHAVIOR ATMICRO LIQUID INTERFACES 1299A. Hibara123, M. Nonaka', S. Toyomura' and T. Kitamori'2J

'University of Tokyo, 2Kanagawa Academy of Science and Technology and3Japan Science and Technology Corporation

3-D IMAGING OF MOVING DROPLETS FOR MICROFLUIDICSUSING OPTICAL COHERENCE TOMOGRAPHY 1303V. Srinivasan, V. Pamula, K. Divakar-Rao, M. Pollack, J.A. Izatt andR. FairDuke University

INTEGRATION OF MULTI-ASPHERICAL LENSES AND OPTICAL FIBERSONTO A PDMS MICROFLUIDIC DEVICE FOR FLUORESCENCE-BASEDDETECTION 1307K. Ono'2, S. Kaneda2, S. Camou2 and T. Fujii2

'Enplas Laboratories, Inc. and 2 University of Tokyo

Concurrent Session - DNA IFAST SEPARATION OF LONG DNA IN A MICROCHIP 1311N. Mine, C. Fiitterer, C. Gosse, KD. Dorfinan and J.-L. ViovyInstitut Curie

FAST SEPARATION OF LARGE DNA BY NANOPILLAR CHIP 1315N. Kaji', Y. Takamura2, Y. Horiike2, H. Nakanishi3, T. Nishimoto3 and Y. Baba14

University ofTokushima, 2 University of Tokyo, 3Shimadzu Corporation and4 National Institute of Advanced Industrial Science and Technology

SELF-CONTAINED, INTEGRATED BIOCHIP SYSTEM FORSAMPLE-TO-ANSWER GENETIC ASSAYS 1319R.H. Liu1, J. Yang', R. Lenigk', J. Bonanno'. P. Grodzinskt and F. Zenhausern''Arizona State University and 2Los Alamos National Laboratory

xlviii

Concurrent Session - Optics IITOTAL INTERNAL REFLECTION-BASED BIOCHIP FOR HIGHTHROUGHPUT BIOASSAYS 1323N. Chronis andL.P. LeeUniversity of California, Berkeley

IMPROVED MICROFLUIDIC DESIGN OF AN ON-CHIP TUNABLE DYE LASER... 1327B. Bilenberg, J.P. Kutter and A. KristensenTechnical University of Denmark

SU-8 BASED SOLID STATE DYE LASERS FOR LAB-ON-A-CHIPMICROSYSTEMS 13315. Kragh, S. Balslev and A. KristensenTechnical University of Denmark

Concurrent Session - DNA IISINGLE MOLECULE AMPLIFICATION IN A CONTINUOUS FLOWLABCHIP DEVICE 1335J. Baker, M. Strachan, K. Swartz, Y. Yurkovetsky, A. Rulison, C. Brooks and A. Kopf-SillCaliper Technologies Corp.

STUDY OF ELECTROCHEMICAL DETECTION OF A SINGLE DNAMOLECULE COUPLED WITH FLUORESCENCE QUENCHING IN MICRO-AND NANOFABRICATED DEVICES 1339M. UedaUniversity ofTokushima

SELECTIVE DNA SENSING ELEMENTS INTEGRATED INTO MICROFLUIDICCHANNELS 1343R.A. Zangmeister and M.J. TarlovNational Institute of Standards and Technology

Author Index B-01

Keyword Index B-15

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