Michelle Anthony Khadija Lawrence. Gram-negative Rod shaped Pathogenic strains ...
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Transcript of Michelle Anthony Khadija Lawrence. Gram-negative Rod shaped Pathogenic strains ...
Escherichia coli
Gram-negative Rod shapedPathogenic strains
http://www.universityofcalifornia.edu/everyday/agriculture/images/e_coli.jpg
The maximum growth of E. coli will be inhibited as the concentration of ampicillin is
increased.
Hypothesis
No titrationsAltered amounts of ampicillin
Varied the ranges each week Results from each week lead to
final experiment
Experiments 5-10
LB H2O E. coli Amp
1 25 224 1 0
2 25 221 1 3
3 25 219 1 5
4 25 215 1 9
5 25 212 1 12
6 25 208 1 16
7 25 204 1 20
8 25 199 1 25
* All values in table are in microL
Experimental Design
1 10 19 28 37 46 55 64 73 82 91 1001091181271361451541631721811901992082172262352442532620
0.2
0.4
0.6
0.8
1
1.2
1.4
E. coli Growth vs Increasing Amounts of Ampicillin
Time (mins)
Grow
th
1612950 3
Maximum growth was not inhibited Normal curve max: 1.088Highest amp max: 1.044
Lag phase dramatically affected after 16microL is exceeded
IC50 not determined Ampicillin affects length of lag phase
Graph consistent with scale
Conclusion derived from Results
http://www.cdc.gov/ecoli/general/http://www.foodsafety.gov/poisoning/causes/b
acteriaviruses/ecoli/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1583696/?page=4
References