Metabolisme Sel FINAL
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Transcript of Metabolisme Sel FINAL
7/24/2019 Metabolisme Sel FINAL
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METABOLISME
SELKELOMPOK 1
Andre Fahriz P.1406605843Shaina Tania1406533661Hasse An!e"n 14065#1$11M. %&s&' Ar"a (. 14065334$1)adina Sa*ia A. 1406533655
(ai'an
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METABOLOMICS
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+EFI)ISI METABOLOMIK
Metabolit
Me,a*-i, adaah sen"aa /iia "an! e*&a, e,a*-isea/anan air dan -*a,2-*a,an &n!/in. Me,a*-ise
ei*a,/an eeahan *ahan -r!ani/ /-e/s "an!/e&dian di!&na/an se*a!ai s&*er ener!i daa ,&*&h. T&*&h en!!&na/an ener!i ini &n,&/ er,&*&hanrer-d&/si dan eeiharaan ,&*&h seara /ese&r&han.Me,a*-i, hadir di se&a ,ahaan r-ses e,a*-ise.Mere/a e*an,& daa eeahan i,ain dan asa
ain- ser,a daa e*an!&nan -e/& /-e/s.
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+EFI)ISI METABOLOMIK
Metabolit
a*ar 1. Br"-s,a,in 1 an,i!en /an/er e,a*-i, B.neri,ina si*i-n *a/,eri 7andida,&s End-*&!&a ser,&a7.
9s&*er: ;&an <HA) dan HE) Ha-. $01$. Metabolomics: Concept, methods and potential prospect in marine biology. h,,:==in/.srin!er.-=ar,ie=10.100#=s11434201$25$3#25 dia/ses
,an!!a $$ )-e*er $015.>
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+EFI)ISI METABOLOMIK
Metabolit da,an! daa ,i!a ?enis @ rier se/&nder dane,a*--es. Ben,&/ &,aa adaah -e/& /ei "an!*er-erasi ada ,in!/a, se. enis se/&nder adaah -e/&"an! e*ih *esar "an! ,eri*a, daa r-ses "an! en?a!a
/eseha,an ,&*&h. Men!hian!/an i*ah a,a& ra&n darisis,e dan en?a!a /eseha,an se2se /&i, "an! ,&!as"an! ei*a,/an e,a*-i, se/&nder. Me,a*--esse*enarn"a ?arin!an e,a*-i, "an! *e/er?a saa den!anrea/si enzi seaa r-ses e,a*-ise. +aa ha*a!aiana ,&*&h ee,a*-ise -*a,2-*a,an ,er,en,&
e,a*-i, ?&!a daa, &n& se*a!ai hasi dari rea/si ,&*&h,erhada s&a,& -*a,.
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+EFI)ISI METABOLOMIK
Metabolisme
Pr-ses e,a*-ise ,erdiri dari seran!/aian ?a&r di anasa,& /iia *er&*ah en?adi ain den!an rea/si enzi "an!
,er?adi di sean?an! ?aan. Enzi eain/an eran en,in!daa en!/--rdinasi/an dan en!a,&r *a!aiana *ahandir-ses seaa r-ses e,a*-ise. Me,a*-i, dan enzi*e/er?a saa sean?an! ?a&r ,erse*&, &n,&/ er-sesn&,risi dan ener!i &n,&/ di!&na/an ,&*&h. Seara ,-,a adase/i,ar $.00 e,a*-i, di/ena saa, ini daa ,&*&h
an&sia.
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+EFI)ISI METABOLOMIK
Metabolisme
+aa se,ia ,aha e,a*-ise ener!i *ai/ di*&a, a,a&di!&na/an &n,&/ ea/sana/an '&n!si *i--!is ,er,en,&.
Lea/ /ar*-hidra, dan r-,ein asin!2asin!die,a*-ise -eh se, "an! *er*eda dari e,a*-i,.Pr-d&/ a/hir dari r-ses e,a*-ise @ seer,i &rea /ar*-ndi-/sida dan r-,ein @ ?&!a ena/& *ahan e,a*-i,"an! r&sa/ di sean?an! ?aan. Pr-ses /ese&r&han ,erdiridari rea/si /a,a*-i/ di ana a,eria dieah dan rea/si
ana*-i/ di ana -e/& /-e/s ,er*en,&/.
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+EFI)ISI METABOLOMIK
Metabonomik dan Metabolomik
Cr&,an en!/a dari !en- an&sia ,eah e*aa *idan!*ar& ada sis,e *i--!i "ai,& -i/. Ka,a D-i/ en!a&ada anaisis "an! /-rehensi' dari sis,e *i--!i,er,en,&. Oi/ adaah /-nse "an! ,erin,e!rasi "an!
en!-*inasi/an se&a in'-rasi "an! dida, dari*er*a!ai ase/ di ana sis,e *i--!i di!&na/an &n,&/en!ines,i!asi 'en-ena *i--!i daa ,in!/a, -e/&er.+i*andin!/an den!an ide--!i *i--!i ,radisi-na -i/,ida/ en"edia/an in'-rasi de,i en!enai eria/& se
a,a& ?arin!an eain/an andan!an && dari r-ses.Oi/ ,ida/ han"a eii/i /e&n!!&an daa eahaanr-ses *i--!is na&n ?&!a r-se/ dia!n-sa "an! e*iha/&ra, er*ai/an daa n&,risi dan *e*eraa ai/asi *ar&"an! ain daa *idan! *i--!i. Be*eraa s&*?e/ en,in!daa eea?ari sen"aa e,a*-is adaah
e,a*-n-i/ dan e,a*--i/.
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+EFI)ISI METABOLOMIK
Metabonomik
Me,a*-n-i/ er,aa di/-nse/an -eh )ih-s-n ada,ah&n 1. Me,a*-n-i/ adaah anaisis /&an,i,a,i' dari
&,iarae,er dinais res-? e,a*-is dari sis,e "an!hid& ,erhada ran!san!an a,-si--!i dan -di/asi!ene,i. Me,a*-n-i/ *er'-/&s ada eahaan sis,e*i--!i dari s,&di sen"aa e,a*-is. Pada aan"ae,a*-n-i/ *er'-/&s ada ai/asi a!ne,i n&/irse/,r-s/-i res-nansi &n,&/ en!anaisa er&*ahan
sen"aa e,a*-is daa airan *i--!is se ?arin!an dan*er,&?&an ada a&!en,asi dan en"es&aian in'-rasi"an! ,ersedia dari en!&/&ran res-n !ene,i/ danr-,e-i ,erhada aaran Gen-*i-,i/.
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+EFI)ISI METABOLOMIK
Metabolomik
Me,a*--i/ adaah s&a,& e,-de ende/a,an &n,&/eahai re!&asi dari methabolic pathways dan ?arin!an
e,a*-i s"s,e sara'. e,a*--i/ een!/ai da,a"an! *erasa dari !en-i/ ,ransri,-i/ dan r-,e-i/&n,&/ e*an,& daa e*eri/an ende/a,an sis,e&n,&/ eea?ari /eseha,an dan en"a/i, an&sia.Me,a*--i/ en!!a*&n!/an s,ra,e!i &n,&/en!iden,i/asi da,a dan en!&/&r e,a*-i, se
en!!&na/an ,e/n--!i anaisis an!!ih den!an eneraane,-de s,a,is,i/ dan &,iarian &n,&/ e/s,ra/si in'-rasidan in,erre,asi da,a.
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+EFI)ISI METABOLOMIK
Defnisi Metabolomik
Me,a*--i/ *erasa dari d&a /a,a: Me,a*-- dan Oi/.Me,a*--i/ "an! dise*&, se*a!ai /e--/ -e/& /ei
daa, dide'enisi/an se*a!ai /ese&r&han e,a*-i, n-n2e,ida den!an *era, -e/& /ei "an! ada di daas&a,& se a,a& -r!anise ada /-ndisi si--!i ,er,en,&"an! di*&,&h/an &n,&/ eeiharaan er,&*&han dan'&n!si n-ra s&a,& se. Me,a*-i, er&a/an hasiin,era/si sis,e !en- den!an in!/&n!ann"a dan *&/an
er&a/an r-d&/ a/hir dari e/sresi !en ,ai ?&!a *erasadari *a!ian sis,e re!&asi daa s&a,& ara "an!,erin,e!rasi dan den!an dei/ian enen,&/an si'a,*i-/iia dan 'en-,i s&a,& se a,a& ?arin!an.
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+EFI)ISI METABOLOMIK
Defnisi Metabolomik
Me,a*--i/ daa, diar,i/an se*a!ai s&a,& anaisis /&an,i,a,i'dan /&ai,a,i' seara en"e&r&h ,erhada se&a -e/&/ei "an! ada di daa s&a,& se ?arin!an a,a& -r!anise,er,en,&. M-e/& /ei ar,n"a -e/& den!an &&ran saaden!an a,a& /&ran! dari 1500 da,-n +a. +en!andei/ian -ier asa ain- dan !ia ,ida/ as&/ /edaa s,&di e,a*--i/.
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+EFI)ISI METABOLOMIK
Defnisi Metabolomik
Me,a*--i/ eii/i /e,er/ai,an den!an /&an,i/asi se&aa,a& eahan *esar dari se&a e,a*-i, di daa s&a,&sae *i--!i dan seara si&,an en!iden,i/asi ser,aen!/&an,i/asi /eas *i--e/&n"a seara *er,&r&,2,&r&,2()A r-,ein dan e,a*-i, Pen!&/&ran e,a*-i,e*eri/an in'-rasi dasar ,en,an! res-n *i--!i,erhada er&*ahan si--!i dan in!/&n!an dan den!andei/ian enin!/a,/an eahaan *i-/iia se&er
se*a!ai ?arin!an ar&s *ai/ e,a*-i, "an! en!a,&re/sresi !en dan r-,ein ser,a eediasi sin"a an,ar-r!anise.
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KO)SEP METABOLOMIK
F-/&s e,a*--i/ adaah anaisis /--sisi za, den!an*era, -e/& rendah daa sae *i--!is. Pende/a,an iniseara ,radisi-na en!!&na/an MS Se/,r-s/-i Massadaa /-*inasi den!an ,e/ni/ eisahan seer,i
/r-a,-!ra !as a,a& /r-a,-!ra airan L &n,&/en!anaisis in'-rasi dari -e/&2*era, rendah sen"aa.
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KO)SEP METABOLOMIK
a*ar $. -n,-h hasi iden,i/asi e,a*--i/ e,-de =MS
9s&*er: The Sa&e (-*er,s )-*e F-&nda,i-n In. $006.Metabolomics.
http://www.noble.org/plantbio/sumner/metabolomics/ diakses tanggal 22 November 20!."
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KO)SEP METABOLOMIK
Cn,&/ en!hindari in,era/si an,ara sen"aa "an! di&/&rdaa a,ri/s ersiaan sae dan anaisis di'-/&s/anada seran!/aian sen"aa *aha an!sa si'a, "an! iri.Iden,i/asi "an! /-rehensi' dan /&an,i/asi sen"aae,a*-i/ disin,esis -eh -r!anise *erar,i e,a*--i/adaah saah sa,& ende/a,an "an! ain! en,in! &n,&/eea?ari e/sresi !ene,i/ dan r-,e-i/.
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KO)SEP METABOLOMIK
Mes/i&n ada /esaaan /-nse an,ara *idan! e,a*-n-i/dan e,a*--i/ ada *e*eraa er*edaan "an! si!ni/andaa ende/a,an dan ,&?&an enei,ian. Se*e&n"a,&?&ann"a didedi/asi/an &n,&/ /-rehensi' e,a*-n-i/r- ada s/aa ,er,en,& sedan!/an "an! ,era/hir,er&,aa *er/ai,an den!an se?arah a/,& ,er!an,&n! r-e,a*-i/ daa s&a,& sis,e "an! ,erin,e!rasi.
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KO)SEP METABOLOMIK
)a&n ,er/ai, den!an en!e*an!an *i-in'-ra,i/a d&a/-nse /e&n!/inan a/an ,erin,e!rasi. Cn,&/ /en"aanan/i,a en!!&na/an "an! e,a*--i/ is,iah daae*ahasan *eri/&,. Or!anise adaah se*&ah sis,e,erin,e!rasi dan ?arin!an den!an ran,ai ,rans-r,asi*i-in'-ra,i/a "an! ,erdiri dari !en2r-,einse,a*-i/sen"aa. en dan r-,ein e*eri/an in'-rasi ,en,an!aa "an! a/an ,er?adi sedan!/an sen"aa e,a*-i/e*eri/an in'-rasi ,en,an! aa "an! ,eah ,er?adi.
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KO)SEP METABOLOMIK
+i*andin!/an den!an -i/ ainn"a e,a*--i/ eii/i/ara/,eris,i/ "an! &ni/ "ai,&:
1. +a,a e,a*-i/ ,ida/ en"edia/an in'-rasi "an!dihasi/an -eh eria/& indeenden dari se ,er,en,& a,a& ?arin!an ,e,ai se*ai/n"a end-/&en,asi/aner&*ahan !-*a e,a*-- *aah !an!!&an e/s,erna"an! enerin/an res-n e,a*-i/ ran!san!an e/s-!endaa andan!an &&.
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KO)SEP METABOLOMIK
$. Sen"aa e,a*-i/ adaah ,i,i/ a/hir dari a/,ii,as !en'&n!si-na. Men&r&, enei,ian -hns-n throughput e,a*-i ,ida/ han"a di/endai/an -eh e/sresi !ene,i/,e,ai ?&!a dises&ai/an -eh in!/&n!an e/s-!en.Per&*ahan /-nsen,rasi sen"aa e,a*-i/ en!!a*ar/anres-n *i--!is ,erhada !an!!&an dari &ar. Oeh /arenai,& sen"aa e,a*-ise e*ih sensi,i' di*andin!/anden!an !en dan r-,ein.
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KO)SEP METABOLOMIK
3. Seaa rea/si *i-/iia /-nsen,rasi enzi dan throughput e,a*-i/ &&n"a ,ida/ *er&*ah sedan!/an /-nsen,rasi*e*eraa -e/& rendah sen"aa den!an *era, a/an*er&*ah seara si!ni/an. Men?adi ,i,i/ a/hir dari ran,ai,rans-r,asi *i-in'-ra,i/a sin"a "an! dihasi/an -eher&*ahan e,a*-- "an! dier*esar dan daa,e*eri/an aasan dan /ara/,erisasi a/&ra, dari '&n!sise.
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METABOLIC PATHWAY
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ALC( METABOLISME
• a&r Me,a*-ise adaah seran!/aian rea/si /iia "an!,er?adi di daa se
• +aa e,a*-ise za, aa e,a*-i, a/an di-di/asi-eh *e*eraa rea/si /iia
• (ea/si ini di/a,ais -eh enzi dan en!hasi/an r-d&/e,a*-ise in,eredia,e "an! a/an di!&na/an &n,&/rea/si sean?&,n"a
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ALC( METABOLISME
• Seara && ?a&r e,a*-ise daa, di*a!i en?adi $ ?enis
• Ana*-ise: ensin,esis -e/& e*&,&h/an ener!i
• Ka,a*-ise: eeah -e/& eeas/an ener!i
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ISTILAH PE)TI)
)O ISTILAH +EFI)ISI
1 (EAKTA) SE)%AJA BIOKIMIA %A) BE(I)TE(AKSI +IAJAL P(OSES +A) AKA) +IKO)E(SI ME)A+ISE)%AJA LAI)
$ P(EKC(SO( SE)%AJA KIMIA %A) ME)A+I AJAL ALC( METABOLIK
3 KATALIS BAHA) KIMIA %A) MEMPE)A(CHI LAC KEEPATA) +A(I(EAKSI KIMIA +E)A) A(A BE(PA(TISIPASI +ALAM (EAKSIS SE+EMIKIA) (CPA SEHI)A KATALIS TE(SEBCT TI+AK
+IKO)SCMSI OLEH (EAKSI
4 E)<IM E)<IM A+ALAH E)IS P(OTEI) %A) MEMAI)KA) PE(A)PE)TI) +ALAM METABOLISME +E)A) A(A ME)KATALISIS
(EAKSI I)+II+C +ALAM ALC( METABOLISME. E)<IM SPESIFIKME)KATALISIS (EAKSI TE(TE)TC.
5 I)TE(ME+IATE SE)%AJA %A) BCKA) AJAL +A(I ALC( METABOLIK MACPC)P(O+CK AKHI( METABOLISME. ME(CPAKA) PE)HCBC)A)TA( (EAKSI +A) AKA) +ICBAH ME)A+I SE)%AJA LAI)
OLEH E)<IM
6 OMMO)
I)TE(ME+IATE
I)TE(ME+IET CMCM A+ALAH SE)%AJA %A) MC)CL +I TITIK
PE(ABA)A) +I ALC( METABOLISME.
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)O ISTILAH +EFI)ISI
# P(O+CK AKHI( SE)%AJA %A) +IP(O+CKSI OLEH (EAKSI METABOLISME %A) TI+AK AKA) BE(PA(TISIPASI +ALAM (EAKSI BE(IKCT)%A
+ALAM ALC( METABOLISME. SEBALIK)%A SE)%AJA I)I +APAT+IC)AKA) ATAC +ISEK(ESIKA) OLEH SEL
8 TITIK PE(ABA)A) TAHAP +I ALC( METABOLIK +I MA)A PE(A)TA(AI)TE(ME+IATE BISA +ILA)CTKA) C)TCK +ICBAH +I LEBIH
+A(I SATC A(A ALC( METABOLISME
PE(SIMPA)A) KC)I TITIK PE(ABA)A) %A) SA)AT PE)TI) SEHI)AME)E)TCKA) KELA)CTA) +A(I ALC( METABOLISME
10 METABOLIT SE)%AJA %A) BE(PA(TISIPASI +ALAM P(OSES METABOLISMEBAIK %A) +IHASILKA) SELAMA (EAKSI METABOLISME ATAC
KO)STITCE)
11 SIKLCS METABOLIK BEBE(APA ALC( METABOLISME ATAC BAIA) +A(I ALC(METABOLISME %A) ME)AMBIL BE)TCK SIKLCS +I MA)AP(O+CK S +A(I (EAKSI METABOLISME I)+II+C +ALAM
C(CTA) ME)A+I (EAKTA) C)TCK (EAKSI METABOLIKBE(IKCT)%A
1$ HCB METABOLIK LOKASI +I MA)A BA)%AK P(OSES METABOLISME TE(A+IMEMC)KI)KA) <AT SEPE(TI MOLEKCL MISAL)%A
I)TE(ME+IET (EAKSI C)TCK ME)T(A)SFE( A)TA(A P(OSES(EAKSI PA+A +ASA( %A) +IBCTCHKA).
SCMBE(
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SCMBE(:HTTPS:==CPLOA+.JIKIME+IA.O(=JIKIPE+IA=OMMO)S=0=0+=METABOLISM#0P;.P)
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ALC( METABOLIK MA)CSIA
• i/-isis 2 -/sidasi !&/-sa &n,&/ endaa,/an ATP
• Si/&s Asa Si,ra, Si/&s Kre*s @ -/sidasi ase,i2-A &n,&/ endaa,/anTP dan in,eredie, *erhar!a.
• F-s'-riasi O/sida,i' 2 e*&an!an ee/,r-n "an! dieas/an -eh !i/-isis
dan si/&s asa si,ra,. Se*a!ian *esar ener!i "an! dieas/an daa r-sesini daa, disian se*a!ai ATP.
• Lin,asan Pen,-sa F-s'a, 2 sin,esis en,-sa dan eeasan /e/&a,an "an!di*&,&h/an &n,&/ en!&ran!i rea/si ana*-i/.
• Si/&s Crea2 e*&an!an daa *en,&/ "an! /&ran! *era&n
• O/sidasi Asa Lea/2N @ eeahan asa ea/ en?adi ase,i2-A "an!a/an di!&na/an -eh si/&s Kre*s .
• &/-ne-!enesis 2 sin,esis !&/-sa dari er/&rs-r /ei "an! a/an di!&na/an-eh -,a/
•
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SCMBE(:HTTP:==JJJ.SIMAAL+(IH.OM=O)TE)T=+AM=SIM
A2AL+(IH=LIFE2SIE)E=BIOHEMIALS=MI(ATIO)BIOHEMIALS1=1
.P
S&*er: h,,:==.si!aadrih.-=-n,en,=da=si!a2adrih=i'e2siene=*i-heias=i!ra,i-n*i-heias1=$3 ?!
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siene=*i-heias=i!ra,i-n*i-heias1=$3.?!
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SCMBE(:HTTP:==PCBS.(S.O(=SE(IES=IMAES=(SPCBS.EPLATFO(M.SE(IE.F(EEO)TE)T.IMAESE(IE.S=IMAESE(IE=A(TILEIMAE=$01
1=MB=0MB00336K=0MB00336K2F1.IF
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METABOLOMICS ANALYSISMETHODS
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Teknik-Teknik Analisis
Metabolomik
Seara !aris *esar ,erdaa, d&a aa e,-de anaisis
e,a*--i/:1) Metabolomik siik !a"i#) Metabolomik $enana kimia
Per*edaan endasar:
%) Metabolomik siik !a"i& A/an eneaah e,a*-ise dari *i-ndi/a,-r seara
/ese&r&han#) Metabolomik $enana kimia&
Han"a ea/&/an /&an,i/asi ,erhada sen"aa ,ar!e,
,er,en,&
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Al'" Penelitian (m'm Teknik Analisis Metabolomik
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" Penelitian (m'm Teknik Analisis Metabolomik Con
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A+ Metabolomik Siik ,a"i Bioinikato"
• Pen!&/&ran dia/&/an den!an en!e/s,ra/ *i-indi/a,-ren!!&na/an ear&, ,er,en,&.
• Be*eraa ,e/ni/ anaisis "an! di!&na/an:
2 Se/,r-s/-i &,rai-e, 2 Se/,r-s/-i in'raerah
2 Se/,r-s/-i assa 2 Se/,r-s/-i ne&,r-n2)M( Nuclear Magnetic
#esonance
2 dan ain se*a!ain"a• Se/,ra assa a,a& r-,-n dari /ese&r&han hasi
e,a*-i, ,erse*&, dir-ses en!!&na/an anaisiss,a,is,i/ 'a/,-ria dan di-, ada !ra/ $rincipalComponent %core P(
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Conto Pen..'naan Metoe MetabolomikSiik ,a"i
P-, $rincipal Component %cores PS ada e/s,ra/ -,-, a /een?ar
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B+ Metabolomik Penana /imia
Bioinikato"
• Han"a en!anaisis sa,& a,a& *e*eraa sen"aa ,ar!e,*i-indi/a,-r.
• Terdaa, *an"a/ sen"aa "an! daa, diai/asi/anse*a!ai enanda /iia.
• Pr-ses aa anaisis enanda /iia ser&a den!an ,e/ni/sidi/ ?ari "ai,& en!e/s,ra/ *i-indi/a,-r en!!&na/anear&, ,er,en,&.
• Sean?&,n"a enanda /iia diisah/an dari e,a*-i, ainen!!&na/an *e*ara ,e/ni/ seer,i: 2 &igh $er'ormance (i)uid Chromatography HPL
2 *as Chromatography 2 dan ain se*a!ain"a
• Kadar dari enanda /iia *erdasar/an &as &na/
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Conto Hasil Analisis Metabolomik Penana /imiaBioinikato"
Men!!&na/an 2MS
2 2
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/0OMATO0A2I
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k"omato."a3 .as adaah ,e/ni/ &n,&/eisah/an sen"aa a,siri daa 'ase!as ea&i 'ase dia.
• Bia 'ase dia *er&a za, ada, /i,ase*&, ara i,& se*a!ai k"omato."a3.as-$aat.
• Bia 'ase dia *er&a za, air /i,ase*&, ara i,& se*a!ai k"omato."a3.as-4ai".
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/"omato."a3 .as
S"ara, &i/an:
Q har&s eii/i /ea,sirian "an! &/&
-a,i
Q s,a*i ,erhada anas.
P-&asi:R 10$0 sen"aa daa, dianaisisden!an /r-a,-!ra !as.
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Sen"aa "an! daa, dianaisis den!anK:
Pada s&h& -erasi-na K U 450-
1. -e/& = sen"aa daa, *er&*ah 'ase!as a,a& &a
$. Tida/ ,erde/--sisi ada s&h&,erse*&,
Den.an katalain
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Ba!ian dasar /r-a,-!ra !as :
1. Sis,e !as e*aa
$. Sis,e eas&/an &i/an
3. Sis,e eanasan /--
4. K--
5. Sis,e de,e/si
6. Sis,e en!-ah da,a
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a!ian dasar /r-a,-!ra !as :
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AS DAN DETE/TO0
Detekto" asPemba5a
asPembaka"
asPen'k'n.
1. T+ He=Ar=)$=H$
$. FI+ He=)$ H$ Cdara
3. FT+ He=)$ H$ Cdara
4. FP+ He=)$ H$ Cdara
5. E+ )$
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AS DAN TE/ANAN
Ti$e as Tekanan as 6an.ib't'kan
1. as Pe*aa # /!=$ a,a& e*ih ,in!!i
$. asPe*a/ar
$ /!=$ a,a& e*ih ,in!!i
3. asPend&/&n! $ /!=$ a,a& e*ih ,in!!i
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• S"ara, !as se*a!ai 'ase !era/ :1. Le*a
$. K-esien di'&si !as rendah
3. Ke&rnian ,in!!i
4. M&dah didaa, dan &rah
5. --/ den!an de,e/,-r "an! dia/ai
• -n,-h !as e*aa : )$ He H$ Ar
d
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/0OMATO0A2I AS
C A 0 A
• Fase !era/: !as2!as *er/e&rnian ,in!!i
• Men!air dari ,a*&n! !as ea&i in?e/,-r as&/ /e daa
/-- /e daa de,e/,-r dan e*&an!an.
• &i/an dias&//an /e in?e/,-r den!an syringe = seri,.
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SISTEM PEMAS(/AN C(PLI/AN
IN,E/TO0)• &i/an har&s dias&/an /e daa /--
se/ai!&s.
• S&h& !er*an! s&n,i/ har&s &/& anas&n,&/ en!&a/an &i/an sedei/ianea, sehin!!a ,ida/ en!hian!/an
/eesienan "an! dise*a*/an -eh araen"&n,i/an.
• Se*ai/n"a har&s &/& rendah &n,&/ene!ah en!&raian a/i*a, anas.
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/0OMATO0A2I AS
C A 0 A
• In?e/,-r er&a/an ,ea, as&/n"a sae /edaa sis,e K
•
dianas/an an,ara 150 $50-
!&naen!&a/an sae dan ear&,n"a.
• Linar&,2inar&, "an! *er'ase &a ini a/andi!era//an /e /-- -eh !as e*aa.
•
K-- *erada daa -en
"an! ,er/-n,r- s&h&n"a.
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/0OMATO0A2I AS
C A 0 A
• La?& i!rasi inar&,2inar&, daa /-- di,en,&/an -eh :si'a,2si'a, si/-/iia ere/a s&h& dan /--sisi /--.
•
+aa /-- inar&,2inar&, ini en!air den!an /eea,an"an! *er*eda2*eda. Linar&, "an! *er!era/ ,erea, a/an/e&ar dari /-- ain! aa dan dii/&,i den!an sisan"a.
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/0OMATO0A2I AS
C A 0 A
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Il'st"asi Analo.i Ca"a /e"!a
/"omato."a3 as C)
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/0OMATO0A2I AS
C A 0 A
• Masin!2asin! inar&, "an! ,ere&si daa/-- a/an eas&/i de,e/,-r.
• S&a,& sin"a is,ri/ a/an ,er*en,&/ a/i*a,dari in,era/si inar&, den!an de,e/,-r.
• Sin"a2sin"a "an! ,er&/&r dire/a -ehs&a,& sis,e da,a dan dira?ah se*a!ai'&n!si a/,& en?adi se*&ahk"omato."am.
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/0OMATO0A2I AS
C A 0 A
• Se*&ah /r-a,-!ra idea e&n"aidere,an &na/ "an! raa, na&n ,ida/
*er,&&//an. Be*eraa &na/ "an!*er,&&//an dinaa/an te"el'sibe"sama.
•
Ja/,& dan &/&ran se*&ah &na/di!&na/an &n,&/ iden,i/asi danen!&/&r /adar sen"aa daa &i/an.
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/0OMATO0A2I AS
C A 0 A
• C/&ran &na/ hasi anaisis *erh&*&n!an*an"a/n"a sen"aa daa &i/an.
• Bia /-nsen,rasi se*&ah sen"aa*er,a*ah a/a &/&ran &na/&ne*esar.
• Bia /-- dan se&a /-ndisi -erasi/r-a,-!ra !as ,e,a saa se*&ahsen"aa a/an en!air daa /--
den!an /eea,an "an! saa.
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/0OMATO0A2I AS
C A 0 A
• Sehin!!a se*&ah sen"aa a/an daa,diiden,i/asi/an -eh a/,& "an!di*&,&h/ann"a &n,&/ *er!era/ daa/-- dinaa/an 5akt' tambat.
• Iden,i/asi sen"aa ,ida/ daa, han"adi,en,&/an sendiri -eh a/,& ,a*a,n"a.
• Sen"aa "an! asi &rni dan di/e,ah&i/adarn"a har&s dianaisis dan a/,&,a*a, ser,a &/&ran a/an didaa,/an.
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•
)iai2niai "an! dier-eh daa,di*andin!/an den!an &i/an "an! ,a/di/ena !&na enen,&/an /e*eradaansen"aa "an! diari den!an
e*andin!/an a/,& ,a*a, dan/adarn"a den!an e*andin!/an&/&ran &na/.
• Bia *e*eraa &na/ *er,&an! ,indiha/a /e,ea,an en!&/&ran &na/2&na/ ini ,ida/ah &n!/in didaa,.
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• Bia d&a *&ah &na/ eii/i a/,&,a*a, "an! saa a/a /e,ea,aniden,i/asi ,ida/ah &n!/in dier-eh.
• Oeh /arena i,& ,ida/ah diin!in/an,er?adin"a &na/ "an! *er,&an! ,indih
a,a& ,ere&si *ersaaan.
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SISTEM DETE/SI DETE/TO0)
Detekto"
Sen6a5a 6an.te"eteksi
,'mlaminim'm
T+ Se&a sen"aa /e&ai
!as e*aa
10 10 n!
FI+ Sen"aa -r!ani/ 01 01 n!
E+ Sen"aa ha-!en=-!a-r!ani/
01 * 01 !
FT+ Sen"aa ni,r-!en='-s'-r-r!ani/
1 * 1 !=
01 * 01 !
FP+ Sen"aa s&'&r='-s'-r-r!ani/
10 * 10 n!=
50 * 50 !
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THE0MAL COND(CTI7ITY
DETECTO0 TCD)Mende,e/si se&asen"aa "an!eii/i
er*edaan *ahan!den!an !ase*aa.
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2LAME IONI8ATION DETECTO02ID)
Sensi,i',erhadasen"aa2
sen"aa-r!ani/ ada&&n"a.
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ELECT0ON CAPT(0E DETECTO0ECD)
Sensi,i' ,erhadasen"aa2sen"aaha-!en dan -!a
-r!ani/.Biasan"a &n,&/anaisis es,isida-r!an-/-rin
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2LAME THE0MIONIC DETECTO02TD 9NPD)
Sensi,i' ,erhadasen"aa '-s'-r-r!ani/ dan
ni,r-!en -r!ani/.Biasan"a &n,&/anaisis es,isidadan r-d&/edi/a.
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2LAME PHOTOMET0ICDETECTO0 2PD)
Sensi,i',erhadasen"aa2
sen"aa'-s'-r-r!ani/s&'&r -r!ani/dan ,iah
-r!ani/.Biasan"a&n,&/ anaisises,isida dana-&r.
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SISTEM PENOLAH DATA
• Sin"a "an! didaa, dari de,e/,-r a/andire/a daa *en,&/ /r-a,-!ra dan
di-ah.
/0OMATO0A2I AS
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/0OMATO0A2I AS
H A S I L
/0OMATO0A2I AS
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/0OMATO0A2I AS
H A S I L
/0OMATO0A2I AS
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H A S I L
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Conto Inte"$"etasi Hasil C9MS
Hasi /r-a,-!ra TI sae da&n ,eh en!!&na/an. Hair 100 &na/ ,erde,e/si. Sen"aa2sen"aa,erse*&, daa, diiden,i/asi en!!&na/an da,a*ase
e,a*-i, =MS dan er&s,a/an )IST.
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Conto Inte"$"etasi Hasil C9MS
C i il C S
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Conto Inte"$"etasi Hasil C9MS
C I i H il C9MS
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Conto Inte"$"etasi Hasil C9MS
C t I t t i H il C9MS
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Conto Inte"$"etasi Hasil C9MS
Pe"beaan C an LC Se4a"a (m'm
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Pe"beaan C an LC Se4a"a (m'm
Ins,r&en ashr-a,h-!rah"
Ins,r&enLiV&id
hr-a,h-!rah"
Pe"beaan C an LC Se4a"a (m'm
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Pe"beaan C an LC Se4a"a (m'm
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Pe"beaan C an LC Se4a"a (m'm
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Pe"beaan C an LC Se4a"a (m'm
Conto $"e$a"asi sam$el a'n te 'nt'k teknik LC9MS an C9MS
METO+E A)ALISIS FI)E(P(I)TI)
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METO+E A)ALISIS FI)E(P(I)TI)
• M&a2&a *er,&?&an &n,&/ e*andin!/an -a a,a&W+ngerprint X dari e,a*-i, "an! *er&*ah seirin!an den!anres-ns ,erhada en"a/i, eaaran ,erhada ,-/sina,erasi in!/&n!an a,a& !ene,i/
• Se,ia za, "an! dianaisis a/an e*eri/an res-n "an!*er*eda
• +aa, diai/asi/an ada *era!a a,ri/s *i--!is seer,i&rin asa a,a& ser&n &dah dan ?arin!an a,a& se
• +aa, diai/asi/an ada *er*a!ai aa e,a*-i, adasaa, "an! *ersaaan
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a*ar 1. +ia!ra /er?a anaisis e,a*-i/n!errin,in!s&*er : h,,:==.n*i.n.nih.!-=
MAAM2MAAM METO+E A)ALISIS FI)E(P(I)T
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MAAM MAAM METO+E A)ALISIS FI)E(P(I)T
• +IMS irect in-ection mass spectroscopy
• )M( Nuclear magnetic resonance Se,r-s-"
• FT2I( ourier transport in'rared se/,r-s/-i
+IMS
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+IMS
• Mer&a/an ,e/ni/ anaisis "an! '-/&s ada en!&/&ranassa dan di!&na/an &n,&/ /&an,i/asi dan iden,i/asien,i,as *i--!is a&&n /iia
• Penen,&an assa -e/& s&a,& e,a*-i, den!an /-reasi
,erhada /--sisi &ns&r e,a*-i, ,erse*&, dan -aassa dari e,a*-i "an! ,er'ra!en,asi den!an /-reasi,erhada s,r&/,&r /iia dia/&/an ea&i e/serien.
• I-n hasi i-nisasi a/an diisah en&r&, rasi- assa
,erhada &a,an =z dan en!hasi/an se*&ah se/,r&assa
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• Tida/ er& dia/&/an rearasi sae
• Sae an!s&n! diin?e/si /edaa aa, anaisis
• Tida/ eer&/an eisahan seara /r-a,-!ra
• Meii/i a?& anaisis "an! ea,
• Meii/i see/,i,as dan sensi,ii,as "an! ,in!!i
KEC)TC)A) +IMS
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KEC)TC)A) +IMS
• Sae ,ida/ er& ea&i en!-ahan
• Tida/ ada sen"aa e,a*-i, "an! hian! dari sae
• Meii/i a/,& anaisis "an! ea,
KE(CIA) +IMS
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KE(CIA) +IMS
• Pada sae den!an /adar !ara "an!,in!!i daa, en"e*a* s&resi i-ner/&&an -e/&2-e/& dan/er&sa/an ada aa,
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)M( SPEKT(OSKOPI
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)M( SPEKT(OSKOPI
• Me,-de ini && di!&na/an &n,&/ en!anaisis biouid
• +aa, dia/&/an ,ana er& en!e,ah&i e,a*-i, "an!dianaisis
• O&,&, *er&a s&er-sisi dari se/,ra dari se&ae,a*-i, "an! ,erdaa, daa sae "an! daa,,erde,e/si
• Me*eri/an /isaran "an! &as e,a*-i, se,ia en!&/&ran
dan e*eri/an in'r-asi ,en,an! s,r&/,&r /iiain!/&n!an /iia er!era/an -e/&ar dinai/ danin,era/si -e/&ar an,ar e,a*-i,
P(I)SIP )M(
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P(I)SIP )M(
Se*&ah n&/e&s di/a,a/an eii/i sin saa, ,erdaa, r-,-na,a& ne&,r-n "an! ,ida/ *erasan!an. )&/e&s "an!*er&a,an a/an en!hasi/an se*&ah di- a!ne,i/sean?an! aGis sin. i/a ada edan a!ne,i/ "an!diai/asi/an a/a daa, ,er?adi ,rans'er ener!i dari ener!i
dasar saai ,in!/a, ener!i "an! e*ih ,in!!i. Trans'er ener!iini ,er?adi ada an?an! !e-*an! daa 're/&ensi radi-.Saa, sin /e*ai /e ener!i dasar a/a ener!i "an! dieaseii/i 're/&ensi "an! saa. Si!na "an! dari ,rans'er ini/e&dian di&/&r dan dir-ses sehin!!a en!hasi/an se*&ah
se/,r& )M(.
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a*ar $. +a,a anaisis )M(s&*er : .di.-
KEC)CLA) )M(
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KEC)CLA) )M(
• Ja/,& r-ses "an! ea,
• Bia"a er sae "an! rendah
• Me,a*-i, "an! dianaisis ,ida/ er& di/e,ah&i se*e&
anaisis
• Tida/ er&sa/ sae
KEKC(A)A) )M(
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KEKC(A)A) )M(
• Meii/i sensi,ii,as "an! rea,i' rendah
• Meii/i res-&si se/,ra "an! rendah
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FT2I( SPEKT(OSKOPI
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• +i!&na/an &n,&/ en!anaisis er&*ahan daa sae *i--!i
• Tida/ daa, ende,e/si se,ia e,a*-i, seara ,erisah
• Ba!ian2*a!ian sesi/ dari -e/& a/an en"era !e-*an!in'raerah ada an?an! !e-*an! ,er,en,&
• Fin!errin, e,a*-i/ didaa,/an dari s&a,& se/,r& a*s-r*sidari se,ia sae
• Se/,r& a*s-r*si *erisis da,a *er*a!ai e,a*-i,
• Ja/,& anaisis : 1 eni,
• Ja/,& anaisis *er!an,&n! ada arae,er ins,r&en
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a*ar 3. Se/,r& FTI(s&*er : $.heis,r".s&.ed&
P(I)SIP FT2I(
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• Ener!i ,-,a daa se*&ah -e/& adaah ?&ah dari,in!/a,an ener!i r-,asi i*rasi dan ee/,r-ni/ dari -e/&,erse*&,
• Meea?ari in,era/si an,ara anai, den!an !e-*an!
ee/,r-a!ne,i/ ada daerah in'ra2erah
• Pada daerah in'ra2erah !e-*an! ee/,r-a!ne,i/seara && *erasan!an den!an i*rasi -e/&ar
•
M-e/& daa, ,ere/si,asi /e /eadaan i*rasi "an! e*ih,in!!i den!an en"era radiasi In'ra2erah
• Me*eri/an hasi +ngerprint ,en,an! /--sisi sae
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METABOLOMICS ANALYSISINTE0P0ETATION
TEK)IK C)TCK ME)I)TE(P(ETASIKA)
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TEK)IK C)TCK ME)I)TE(P(ETASIKA)+ATA HASIL A)ALISIS METOBOLOMIK
+aa e,a*--i/ dier&/an e,-des,a,is,i "an! en!/-er ra,&san2ri*&an
da,a. In'-rasi niai heia shi', *era,-e/& ,er,en,& BM dan in,ensi,as &na/,ida/ *era/na ?i/a ,ida/ dia/&/anin,e!rasi den!an da,a ain dan san!a, s&i,
?i/a en!anaisis er*edaan an,arsaeden!an en!-*serasi ran!e da,a danerda,a. Cn,&/ i,& s,a,is,i har&s dii*a,/an.
TEK)IK C)TCK ME)I)TE(P(ETASIKA)
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+ATA HASIL A)ALISIS METOBOLOMIK
S,a,is,i/ &,iaria,e "an! serin! di!&na/anadaah PA Prinia --nen, Ana"sis
"an! en!hasi/an &aran eisahan da,a,ana s&erise na&n an!s&n! /arenasae asin!2asin!. Ke&diandian?&,/an den!an e,-de s&erise PLS
Par,ia Leas, SV&are "an! a/aneeriha,/an /-n,ri*&si dari /--nendaa sae sen"aa.
P(I)IPLE OMPO)E)T A)AL%SIS=PA
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PA adaah ,e/hni/ /asi/asi "an!en!&*ah da,a2da,a "an! /-e/s
en?adi ,aian "an! e*ih sederhanaden!an en!&*ah r-er," en,in! da,a/edaa s&*& ; dan %. +&a /e--/"an! dias&/an daa PA
en!indi/asi/an er*edaan e,a*-i, "an!si!ni/an.
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KEC)TC)A) PE)C)AA) P(I)IPAL OMPO)E)T A)AL%SIS
PA +IBA)+I)KA) METO+E LAI):
• +aa, en!hian!/an /-reasi seara *ersih /-reasi Y 0sehin!!a asaah &,i/-ineari,as daa, *enar2*enar
,era,asi seara *ersih
• +aa, di!&na/an &n,&/ se!aa /-ndisi da,a = enei,ian
• +aa, dier!&na/an ,ana en!&ran!i ?&ah aria*e asa
• Jaa&&n e,-de (e!resi den!an PA ini eii/i ,in!/a,/es&i,an "an! ,in!!i a/an ,e,ai /esi&an "an! di*eri/ane*ih a/&ra, di*andin!/an den!an en!&naan e,-de ain.
PA(TIAL LEAST SZCA(ES
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+IS(IMI)A)T A)AL%SIS PLS2+A
• PLS2+A di!&na/an &n,&/ enin!/a,/an eisahanan,ara /ed&a /e--/ den!an eran!/& da,a
/edaa *e*eraa aria*e a,en "an! a/anea/sia/an -arian an,ara res-n danredi/,-r.
• Cn,&/ eer/ei niai /e&n!/inan "an!dise*a*/an -eh /e*e,&an dari eisahan PLS2+A
a/a dia/&/an &?i er&,asi. C?i ini ,erdiri a,asen!&an!an da,a sain! $000G den!an a*eaa/ "an! *er*eda.
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METABOLOMICSAPPLICATIONS
APLIKASI METABOLOMIK
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• C/&ran !en- ,rans/ri,- dan r-,e- dier/ira/ansedi/i, e*ih *esar di*andin!/an e,a*-- dan san!a,/-nsera,i' ada *er*a!ai -r!anise.
• +id&!a ada /&ran! e*ih $5.000 !en 100.000 ,rans/ri dan
e*ih dari sa,& ?&,a r-,ein ada an&sia a/an ,e,aihan"a se/i,ar $.500 e,a*-i, daa e,a*-- an&sia.
APLIKASI METABOLOMIK +ALAM ILMC TA)AMA)
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• Beran'aa, daa en!e*an!an e,a*-i, se/&nder a/,i' dari ,anaan-*a, se*a!ai an!en ,-,erai *ar& a,a& "an! di/e*an!/an.
• +ian'aa,/an daa /a?ian ,er/ai, a/&&asi *i-assa resis,ensi ,erhadas,res dan r-d&/si e,a*-i, se/&nder.
• Menaar/an ara "an! ea, &n,&/ en!&n!/a '&n!si !en "an! *e&di/e,ah&i &,asi !ene,i/ dia/&/an di daa sis,e den!an en!anaisa*ahan /iia.
• +asar e,a*-i/ er*andin!an adaah ei*a,an /e daa ,e/n--!i *ar&&n,&/ e-ni,-r er/e*an!an en"a/i, e,a*-ise -*a, dan ,-/si/--!i/iia
APLIKASI METABOLOMIK PA+A CI AKTIITAS A)TIBAKTE(I
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• Ekstrak dan fraksi dilarutkan dalam DMSO dengan konsentrasi 400
mg/ml.
• Kultur bakteri Staphylococcus aureus diambil satu ose dan dilarutkan
dalam media ! "0 ml dan diinkubasi #$ selama %4 &am
• Sebanyak 0'( ml suspensi bakteri tersebut kemudian dituang ke dalam (00
ml media ) yang telah disterilisasi lalu dituang ke dalam ca*an
sebanyak + %( ml
• DMSO digunakan sebagai kontrol negatif dan chloramphenicol ,%(
mg/ml- digunakan sebagai kontrol positif. Media kemudian diinkubasi
pada suhu # o $ selama 4 &am.
•
(EFE(E)SIA. A. Miranda %. A. Le B-r!ne and . B-n,ei. New #outes 'rom Minimal 1pproimation
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! pp3rror to $rincipal Components -&e $# )&*er 3 = &ne $008 )e&ra Pr-essin!Le,,ers Srin!er
An-ni. $00#. Considerations 'or %electing *C/M% or (C/M% 'or Metabolomics. CSA:A!ien, Tehn--!ies
An-ni. F-&rier Trans'-r In'ra2(ed FTI( Se,r-s-". Onine. Tersedia di:h,,:==.h"sis.n&s.ed&.s!=L3000=Lee3an&as=FTI(.d'. 9+ia/ses ada $3)-e*er $015>
An-ni. $015. Prinies -' )M(. Onine. Tersedia di:h,,:==.h.i.a.&/=-a=-r!ani=nrrinies.h,. 9+ia/ses ada $4 )-e*er$015>.
An-ni. $015. Jha, is )M([. Onine. Tersedia di:
h,,:==he.h.h&?i.a.i=nr=ha,isnr=ha,isnr.h,. 9+ia/ses ada $3 )-e*er$015>.
Bi--!"2-nine.-r!. $008. Metabolic $athway. Onine. Tersedia di:h,,:==.*i--!"2-nine.-r!=di,i-nar"=Me,a*-ia,ha". 9+ia/ses ada $4)-e*er $015>
+e,,er Ka,?a Ar-n- Pae A. dan Ha-/ Br&e +.. $015. Mass %pectroscopy4basedMetabolomics. Onine. Tersedia di:h,,:==.n*i.n.nih.!-==ar,ies=PM10433#=. 9+ia/ses ada $3 )-e*er$015>.
+raer )-ran \ Si,h Harr". 1$. 1nalisis #egresi 5erapan. a/ar,a: PT raediaP&s,a/a C,aa.
Eis +aid I. d//.. $00#. Metabolic ingerprinting as a iagnostic 5ool. Onine. Tersediadi: h,,:==d*/!r-&.-r!=daees=Phara-!en-is.d'. 9+ia/ses ada $4 )-e*er$015>.
F&/&sa/i E. $ossibilities and 5echnological 6bstacles in Metabolomics . Bi-,ehn--!"
$006 84 $312$34.
(EFE(E)SIKE. Metabolic $athways Map. Onine. Tersedia di:
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h,,:==.!en-e.?=/e!!2*in=sh-a,ha"[a01100 . 9+ia/ses ada $4 )-e*er $015>
K-sides A"ssa K. d//.. $013. Metabolomic ingerprinting: Challenges and 6pportunities. Onine.
Tersedia di: h,,:==.n*i.n.nih.!-==ar,ies=PM406$40=. 9+ia/ses ada $4 )-e*er $015>.
)iesen ens dan Mihae . ee,,. $00# . Metabolomics: 1 $ower'ul 5ool in %ystems 9iology . +enar/:
Srin!er.
P-n!s&an J. F&/&sa/i E. Ba*a T. K-*a"ashi A. $rediction o' apanese *reen 5ea #anking by *as
Chromatography/Mass %pectrometry49ased &ydrophilic Metabolite ingerprinting . . A!ri. F--d he.
$00# 55 $312$36.
(-essner C,e dan air&s B-ne1. $00. ;hat is metabolomics all about<. Onine. Tersedia di:
h,,:==.*i-,ehniV&es.-=&,iedia=arhie=00044=BT)A000113133O44653a.d' . 9+ia/ses
,an!!a $$ )-e*er $015>
(-se I". $01$. Metabolic $athways. Onine. Tersedia di:h,,:==.i"r-ses.-=H&anBi--!"=Me,a*-is=e,a*-i2a,ha"s.h . 9+ia/ses ada $4
)-e*er $015>
S,&d".-. Metabolic $athway. Onine. Tersedia di:
http://study.com/academy/lesson/what4is4a4metabolic4pathway4de+nition4eample.html. =iakses pada
27 November 20!"
Cniersi," -' Birin!ha and Briin!ha Me,a*--is Trainin! en,re. $015. 6ther 1nalytical
5echni)ues 1pplied >n Metabolomics. Onine. Tersedia di:h,,s:==.'&,&reearn.-=-&rses=e,a*--is=1=s,es=5$45$. 9+ia/ses ada $3 )-e*er $015>.
ias2B-as S . Sar, K F. Sia/&aran S. Lane A. 1lkylation or %ilylation 'or 1nalysis o' 1mino and
Non41mino 6rganic 1cids by *C/M%. Me,a*-i,es $011 11 32$0.
;&an <HA) dan HE) Ha-. $01$. Metabolomics: Concept, methods and potential prospect in marine