Meristem organization How do meristems maintain themselves?
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Transcript of Meristem organization How do meristems maintain themselves?
Meristem organization
How do meristems maintain themselves?
0d 1d 3d 5d
Effect of ablation of quiescent center (QC) on tissue-specific marker gene expression
(from Sabatini et al., 1999, Cell 99: 463-472)
Vegetative shoot apical meristem (SAM)
Vegetative SAM
Inflorescence SAM
Flower meristems
Leaves, branches (lateral meristems)
Sepals, petals, stamens, carpels
Induction of flowering
Inflorescence meristem:
Makes flowers instead of leaves and branches
Stem elongates more
Flower meristems
Shoot apical meristem (SAM)
Arabidopsis thaliana: model plant to study development
Other model plants
Arabidopsis thaliana (Mouse-ear cress, Thale cress)
Small weed, related to mustard - can fit thousands of plants in a small growth room Short generation time: 6-8 weeks Diploid, self-pollinating, transformable
Small sequenced genome: About 125 Mb and 25,000 genes
Arabidopsis is closely related to these plants
Finding Arabidopsis mutants
EMS (ethylmethane
sulfonate)
3. Screen M2 plants for mutants having
phenotypes of interest
2. Grow M1 plants (let self-fertilize and collect M2 seed)
1. Mutagenize seeds (M1)
Vegetative shoot apical meristem (SAM)
(from Long et al., 1996, Nature 379: 66-69)
Wild-type shoot apex shootmeristemless apex
Gene expression in embryonic SAM
WUSCHEL (WUS) SHOOTMERISTEMLESS (STM)
(from Laux et al., 1998, Cell 95: 805-815)
wuschel mutant
(from Laux et al., 1998, Cell 95: 805-815)
Wild type
Gene expression in embryo SAM
WUSCHEL (WUS) SHOOTMERISTEMLESS (STM)
(from Laux et al., 1998, Cell 95: 805-815)
WUS expression in adult shoot meristems
(from Laux et al., 1998, Cell 95: 805-815)
(from J. C. Fletcher et al., Science 283, 1911 -1914 (1999))
Wild type clavata3 mutant
clv1 and clv3 mutants look the same
Expression of CLV1 in Arabidopsis inflorescence shoot meristem
Clark et al., Cell 89: 575-585 (1997)
CLV3 (A-D) and CLV1 (E) expression
(from J. C. Fletcher et al., Science 283, 1911 -1914 (1999))
CLV3 expression in a clv1 mutant meristem
(from J. C. Fletcher et al., Science 283, 1911 -1914 (1999))
Support for model:
In clv mutant, WUS expression domain expands.
clv wus double mutants resemble wus single mutants.
If you overexpress WUS, CLV genes are activated and new meristems form.
Vegetative SAM
Inflorescence SAM
Flower meristems
Leaves, branches (lateral meristems)
Sepals, petals, stamens, carpels
Induction of flowering
Vegetative SAM (indeterminate)
Inflorescence SAM (indeterminate)
Flower meristems (determinate)
Leaves, branches (lateral meristems)
Sepals, petals, stamens, carpels
Induction of flowering
Flower meristems
Shoot apical meristem (SAM)
Arabidopsis flower
sepal
stamen
carpelpetal