MelanoBronze Natural Tanning Activator - AVG Personal … · • MelanoBronze is a combination of...

© Mibelle Biochemistry, Switzerland 2007

MelanoBronzeNatural Tanning Activator


MelanoBronze

Acetyl tyrosine Substrate for tyrosinase enzyme in melanin synthesis

Vitex agnus-castusextract

Induction of melanin synthesis in melanocytes


• MelanoBronze is a combination of two actives leading to an accelerated melanin formation in the skin

• Melanin is the body‘s own sun protecting system preventingsun burn and photo aging in the skin

• MelanoBronze stimulates skin tanning even without sunexposure

MelanoBronze


Melanocyte

Melanosome

Melanocyte

Dendrite of melanocyte

Keratinocyte

Cell nucleus

Basal layer

Dermis

Stratum corneum

Stratum granulosum

Stratum spinosum

Epidermis


Mechanism of Skin Pigmentation

tyrosin DOPA

pheomelanin

DOPA quinone

α MSH

MC1R

cAMP

Cysteinyl DOPADOPA chrom

DHICA

eumelanin

tyrosinase

melanocyte


• Stable, water-soluble substrate for tyrosinase

• The reaction of tyrosine to DOPA by tyrosinase is the rate-limiting step in melanin formation.

• Result: tanned skin and increased UV self-protection of the skin

Acetyl Tyrosine

acetyl tyrosine DOPA melanintyrosinase


The Role of α-MSH on Tanning

αMSHpituitarygland

blood-stream

tyrosine melanintyrosinase

Receptor binding→ induction of melaninsynthesis


N C

proopiomelanocortin (POMC)

ACTH beta-endorphin

αMSH

267 amino acid residues

39 31

13

Processing of POMC


The beta-endorphin/μ-opiate receptorsystem is expressed in melanocytes and closely associated withmelanosomes

Beta-Endorphin and Pigmentation

Melanocyte dendricity, proliferation and pigmentationare upregulated

Kauser et al. 2003 Journal of Investigative Dermatology

beta-endorphin

μ-opiate receptor


Monk's Pepper Berries ContainPhyto-Endorphins

Chaste tree (Vitex agnus-castus)

• Large shrub growing in mediterranean region and Asia

• Berries from Monk's pepper contain substances that bind to the opioid receptor (Phyto-Endorphins)


Monk‘s Pepper BerriesTraditional Medicine

• Used since ancient Greek to treat injuries and inflammation

• In the middle ages, monks used dried berries. They probably experienced a sensation of well-beeing


Binding to the Opioid Receptor

opioid receptor

μ κ δ

36 22 194

B. Meier et al. (2000) Phytomedicine 7(5): 373-381

Inhibition of opioid receptorbinding by an ethanolic extractof Vitex agnus-castus expressed as IC50-values (μg/ml)


MelanoBronze: Composition

Vitex agnus-castus extract containing:

Acetyl Tyrosine 6%Glycerin 20%Alcohol 20%


MelanoBronze

Natural tanning induced by two mechanisms:


MelanoBronze

phyto-endorphin

MelanoBronze


MelanoBronze

phyto-endorphin

MelanoBronze

acetyl tyrosine


MelanoBronze

phyto-endorphin

MelanoBronze

acetyl tyrosine

melanin


MelanoBronzefor Pigmentation with Sun

αMSH tyrosinase pigmentation

αMSH beta-endorphin activity

tyrosinase pigmentation

UV+ MelanoBronze

UV

+ acetyl tyrosine


MelanoBronzefor Pigmentation without Sun

MelanoBronze

beta-endorphin activity pigmentationtyrosinase

+ acetyl tyrosine

Acetyl Tyrosine

no pigmentation !+ acetyl tyrosine

no tyrosinase activity !


In Vitro: Specific Stimulation of Melanin Synthesis in Melanocytes

0

10

20

30

40

50

60

Control 0.03% 0.13% 0.06%

Vitex agnus-castus in the cultur medium

Mel

anin

(ug

/ml)

+45%

S-091


In VivoStimulation of Skin Tanning

Study design• Test products: test cream

cremes with 2% and 5% MelanoBronzecreme with only acetyl tyrosine (same concentration as in 5% MelanoBronze)

• Number of test persons: 20 of age 20 to 55• Application: twice daily for 7 days on the inner side of forearm• Irradiation: every day with 1.1 MED• Test parameter: skin tanning (Chromameter)


Result

Double tanning effect of acetyl tyrosine and Vitex agnus-castus extract

0

0.5

1

1.5

2

2.5

3

3.5

4

4.5

Control cream 2% MelanoBronze 5% MelanoBronzeTann

ing

eff

ect (

dec

reas

e in

chr

om

amet

er u

nits

rela

tive

to b

asel

ine

%)

S-106


MelanoBronze ↔ DHA

MelanoBronze DHA(common tanning ingredient)

Stimulation of melanin synthesisin melanocytes

Browning of the skin surface by a chemical reaction of DHA withproteins (Maillard reaction)

Protection against solar irradiation No protection against solar irradiation

Slow but long lasting effect Fast but irregular browning


Tanning without Sun (after 28d)

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

Placebo 2% MelanoBronze 5% MelanoBronze

Chr

om

amet

er re

adin

g d

28

– d

0 [

a.u.

]

S-129


MelanoBronzeProtective Tanning Enhancer

ClaimsMelanoBronze provides nice regular tanning by stimulating the natural melanin formation

- with UV irradiation- without UV irradiation

MelanoBronze leads to enhanced pigmentation and protects your skin against UV-induced damage.


Applications

Daycreams• MelanoBronze gives a natural tan and

protects against photo-agingSun protection products• Sunscreens normally do prevent the

formation of a rapid tanning• MelanoBronze in sun protection products

speeds up the tanning processSelf tanning products• MelanoBronze induces the skin's own

melanin formation, enforcing your selftanning products

MelanoBronze Natural Tanning Activator - AVG Personal … · • MelanoBronze is a combination of...

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