MEIOSISMEIOSIS

Meiosis

• The form of cell divisioncell division by which gametes, gametes, with halfhalf the number of chromosomes,chromosomes, are produced.

• Diploid (2n) Diploid (2n) haploid (n)haploid (n)

• MeiosisMeiosis is sexual reproduction.sexual reproduction.

• Two divisionsTwo divisions (meiosis I meiosis I and meiosis IImeiosis II).

FertilizationFertilization

• The fusion of a spermsperm and eggegg to form a zygotezygote.• A zygote is a fertilized egg

n=23egg

sperm n=23

2n=46zygote

MeiosisMeiosis

• Sex cells Sex cells divide to produce gametesgametes (sperm (sperm or egg)or egg).

• GametesGametes have halfhalf the # of chromosomeschromosomes.

• Occurs only in gonads (testes or ovaries).Occurs only in gonads (testes or ovaries).

Male: spermatogenesisMale: spermatogenesis

Female: oogenesisFemale: oogenesis

• MeiosisMeiosis is similar to mitosismitosis with some chromosomal differences.

SpermatogenesisSpermatogenesis

2n=46

humansex cell

diploid (2n)

n=23

n=23

meiosis I

n=23

n=23

n=23

n=23

sperm

haploid (n)

meiosis II

Meiosis – mouse testes Meiosis – mouse testes Parent cell

4 gametes

1st division

2nd division

Interphase IInterphase I

• Similar to mitosismitosis interphase.

• ChromosomesChromosomes replicate (S phase).(S phase).

• Each duplicated chromosomechromosome consist of two identical sister chromatidschromatids attached at their centromerescentromeres.

• CentrioleCentriole pairs also replicate.

Interphase IInterphase I

• NucleusNucleus and nucleolusnucleolus visible.

nuclear membrane

nucleolus

cell membrane

chromatin

Meiosis I (four phases)Meiosis I (four phases)

• Cell division Cell division that reduces the chromosomechromosome number by one-half.one-half.

• four phasesfour phases:

a.a. prophase Iprophase I

b.b. metaphase Imetaphase I

c.c. anaphase Ianaphase I

d.d. telophase Itelophase I

Prophase IProphase I

• Longest and most complex phase (90%).Longest and most complex phase (90%).

• ChromosomesChromosomes condense.

• SynapsisSynapsis occurs: homologous chromosomes homologous chromosomes come together to form a tetradtetrad.

• TetradTetrad is two chromosomeschromosomes or four chromatidschromatids (sister and nonsister chromatids).

Prophase IProphase I - - SynapsisSynapsis

Homologous chromosomes

sister chromatids sister chromatidsTetrad

Homologous ChromosomesHomologous Chromosomes

• Pair of chromosomeschromosomes (maternalmaternal and paternalpaternal) that are similar in shape and size.

• Homologous pairs (tetrads) (tetrads) carry genes controlling the same inherited traits.

• Each locuslocus (position of a gene) (position of a gene) is in the same position on homologues.

• Humans have 23 pairs of homologous chromosomes.homologous chromosomes.

a. 22 pairs of autosomesautosomes

b. 01 pair of sex chromosomessex chromosomes

KaryotypeKaryotype• A method of organizing the A method of organizing the chromosomes chromosomes of a of a

cell in relation to number, size, and type.cell in relation to number, size, and type.

Homologous ChromosomesHomologous Chromosomes

Paternal Maternal

eye color locus

eye color locus

hair color locus

hair color locus

Humans have 23 Sets of Homologous ChromosomesEach Homologous set is made up of 2 Homologues.

Homologue

Homologue

AutosomesAutosomes(The Autosomes code for most of the offspring’s traits)(The Autosomes code for most of the offspring’s traits)

In Humans the “Autosomes” are sets 1 - 22

21 trisomy – Downs 21 trisomy – Downs SyndromeSyndrome

Can you see the extra 21st chromosome?

Is this person male or female?

Sex ChromosomesSex ChromosomesThe Sex Chromosomes code for the sex of the offspring.The Sex Chromosomes code for the sex of the offspring.

** If the offspring has two “X” chromosomes it will be a ** If the offspring has two “X” chromosomes it will be a femalefemale.. ** If the offspring has one “X” chromosome and one “Y” ** If the offspring has one “X” chromosome and one “Y”

chromosome it will be a chromosome it will be a malemale..

XX chromosome - female XY chromosome - male

In Humans the “Sex

Chromosomes” are the 23rd set

Boy or Girl? The Y Chromosome “Decides”Boy or Girl? The Y Chromosome “Decides”

X chromosomeX chromosomeY chromosomeY chromosome

Crossing OverCrossing Over

• Crossing over (variation) Crossing over (variation) may occur between nonsister chromatidschromatids at the chiasmatachiasmata.

• Crossing overCrossing over: segments of nonsister chromatidschromatids break and reattach to the other chromatidchromatid.

• Chiasmata (chiasma) Chiasmata (chiasma) are the sites of crossing crossing overover.

Crossing Over - variation Crossing Over - variation

nonsister chromatids

chiasmata: site of crossing over

variation

Tetrad

Another Way Meiosis Makes Lots of Another Way Meiosis Makes Lots of Different Sex Cells – Crossing-OverDifferent Sex Cells – Crossing-Over

Crossing-over multiplies the already huge number of Crossing-over multiplies the already huge number of different gamete types produced by independent different gamete types produced by independent

assortment.assortment.

Sex ChromosomesSex Chromosomes

XX chromosome - female XY chromosome - male

Prophase IProphase I

centriolesspindle fiber

asterfibers

Metaphase IMetaphase I• Shortest phaseShortest phase

• TetradsTetrads align on the metaphase platemetaphase plate.

• INDEPENDENT ASSORTMENT OCCURS:INDEPENDENT ASSORTMENT OCCURS:1. Orientation of homologous pair to poles is random.2. Variation3. Formula: 2n

Example:Example: 2n = 42n = 4then then n = 2 n = 2

thusthus 2 22 2 = 4 combinations= 4 combinations

Metaphase IMetaphase I

metaphase plate

OR

metaphase plate

Question:Question:

• In terms of In terms of Independent AssortmentIndependent Assortment - -how many different combinations of how many different combinations of sperm could a sperm could a humanhuman malemale produce? produce?

AnswerAnswer

• Formula: 2Formula: 2nn

• Human chromosomes:Human chromosomes: 2n = 462n = 46

n = 23n = 23

• 222323 = ~8 million combinations = ~8 million combinations

Anaphase IAnaphase I

• Homologous chromosomes Homologous chromosomes separate and move towards the poles.

• Sister chromatids Sister chromatids remain attached at their centromerescentromeres.

Anaphase IAnaphase I

Telophase ITelophase I

• Each pole now has haploidhaploid set of chromosomeschromosomes.

• CytokinesisCytokinesis occurs and two haploid daughter cells are formed.

Telophase ITelophase I

Meiosis IIMeiosis II

• No interphase II No interphase II

(or very short - no more DNA replicationDNA replication)

• Remember:Remember: Meiosis II Meiosis II is similar to mitosismitosis

Prophase IIProphase II

• same as prophaseprophase in mitosismitosis

Metaphase IIMetaphase II

• same as metaphasemetaphase in mitosismitosis

metaphase platemetaphase plate

Anaphase IIAnaphase II

• same as anaphaseanaphase in mitosismitosis

• sister chromatids separatesister chromatids separate

Telophase IITelophase II

• Same as telophasetelophase in mitosismitosis.

• Nuclei form.

• CytokinesisCytokinesis occurs.

• Remember:Remember: four haploid daughter cells four haploid daughter cells produced.produced.

gametes = sperm or egggametes = sperm or egg

Telophase IITelophase II

MeiosisMeiosis

2n=4

sex cell

diploid (2n)

n=2

n=2

meiosis I

n=2

n=2

n=2

n=2

sperm

haploid (n)

meiosis II

VariationVariation

• Important to population as the raw Important to population as the raw material for material for natural selectionnatural selection..

• Question:Question:

What are the three sexual sources ofWhat are the three sexual sources of

genetic variation? genetic variation?

Answer:Answer:

1. crossing over (prophase I)1. crossing over (prophase I)

2. independent assortment (metaphase I)2. independent assortment (metaphase I)

3. random fertilization3. random fertilization

Remember: variation is good!

Question:Question:

• A cell containing 20 chromosomes20 chromosomes (diploid)(diploid) at the beginning of meiosis would, at its completion, produce cells containing how many chromosomeschromosomes?

Answer:Answer:

• 10 chromosomes (haploid)10 chromosomes (haploid)

FertilizationFertilization

• The fusion of a spermsperm and eggegg to form a zygotezygote.• A zygote is a fertilized egg

n=23egg

sperm n=23

2n=46zygote

Question:Question:

• A cell containing 40 chromatids40 chromatids at the beginning of meiosis would, at its completion, produce cells containing how many chromosomeschromosomes?

Answer:Answer:

• 10 chromosomes10 chromosomes

Sources

• www.ursulinehs.org/powerpoint/meiosis.ppt

• www.biology4teachers.com/Cell%20Division/MEIOSIS.ppt

• http://www.iteachbio.com/Life%20Science/LifeFunctionsandTheCell/Meiosis.ppt