Mechanical Properties of Collagen Molecule
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Transcript of Mechanical Properties of Collagen Molecule
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Mechanical Properties of Proteins
Swapnil Khachane
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Take Away
1. What are proteins
2. Necessity of the study
3. Sarcomere and Titin
4. Go-like Model
5.Results
6.Summary
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Purpose of the Study
• Diseases
• Injury/Trauma
• Future Inventions
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Titin
From. Cieplak 2002Figure 1. Structure of Titin
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Titin
Figure 2. Structure of TitinFrom. Tshovorebova 2005
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Sarcomere in a Striated Muscle
From. Cieplak 2002Figure 3. Sarcomere
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Go Like Model
From. Buehler 2008
• Cα Atoms
• Lennard Jones Potential
• Equation of Motion
612
ij 24 ij
ij
ij
ij
rrE
randFFcrrm
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Results from the model
Figure 4. Force Displacement Curve for 1titFrom. Cieplak 2005
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Experimental Results
Figure 5. Force Displacement Curve for 3tit
From. Tshovorebova 2005
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Experimental Results
Figure 6. Force Displacement Curve for 3tit
From. Tshovorebova 2005
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References•Sulkowska et al., Predicting the order in which contacts are broken during single molecule protein stretching experiments, Wiley-Liss Inc, 2007.•Cieplak et al. , Folding and Stretching in a Go-like Model of Titin.•Cieplak et al,. Stretching of proteins in the entropic limit. Phys Rev e 2004; 69: 011912.•Erickson HP. Protein biophysics - stretching single protein molecules: titin is a weird spring. Science 1997; 276: 1090-1092.•Tskhovrebova et al, Elasticity and unfolding of single molecules of the giant muscle protein titin. Nature 1997; 387: 308-312.•Dietz H,Berkemeier F,Bertz M,Rief M. Anisotropic deformation response of single protein molecules. Proc Natl Acad Sci USA 2006; 103: 12724-12728.•West et al, Mechanical Resistance of Proteins Explained Using Simple Molecular Models, Biophysical Journal, Volume 90, Issue 1, 287-297, 1 January 2006.•Ceiplak et al, Pulling single bacteriorhodopsin out of a membrane: Comparison of simulation and experiment, Biochimica et Biophysica Acta (BBA) - Biomembranes•Volume 1758, Issue 4, April 2006, Pages 537-544.•Kil Ho Eom, The Mechanical Modeling of Proteins.•Yoon et al, Mesoscopic Model for Mechanical Characterization of Biological Protein Materials, Nano-Bio Research Center, Korea Institute of Science & Technology (KIST), Seoul 136-791, Republic of Korea.
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Thank You