Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify...

5
December 13 Material Copyright, Lyndsay S. Durham 1 That is NOT a Rice Krispy … Lyndsay Durham, MEd Department of Forensic Science December 13 Material Copyright, L. Durham FORENSIC ENTOMOLOGY The study of insects and their arthropod relatives that interact with legal matters. It is based on the analysis of insects sequentially colonizing a corpse as decomposition progresses and on the developmental stages of their offspring. December 13 Material Copyright, L. Durham History of FORENSIC ENTOMOLOGY First actual case: 1235AD. The Washing Away of Wrongs Spontaneous generation of maggots in meat was disproved in 1668. First published account in 1855 in the West (France). December 13 Material Copyright, L. Durham Mateu Orfila 1787-1853 Father of Modern Toxicology First systematic observations of insect successions on human cadavers (1848) History of FORENSIC ENTOMOLOGY December 13 Material Copyright, L. Durham History of FORENSIC ENTOMOLOGY Jean Pierre Megnin 1828 - 1905 Wrote the founding works of modern forensic entomology: Fauna of Tombs (1887) Fauna of Cadavers (1894) Wrote a series of articles (1883 – 1896) that made the information accessible December 13 Material Copyright, L. Durham Applications Estimation of PMI Determination of death location Criminal Misuse Abuse of young & elderly DNA Analysis Toxicological specimens Scene characterization Scope of FORENSIC ENTOMOLOGY December 13 Material Copyright, L. Durham

Transcript of Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify...

Page 1: Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify FIRST: Eyes, ears, nose, mouth In the event of anti-mortem trauma, flies ... The study

December 13

Material Copyright, Lyndsay S. Durham 1

That is NOT a Rice Krispy …

Lyndsay Durham, MEdDepartment of Forensic Science

December 13

Material Copyright, L. Durham

FORENSIC ENTOMOLOGY

The study of insects and their arthropod relatives that interact with legal

matters.

It is based on the analysisof insects sequentiallycolonizing a corpse as

decomposition progressesand on the developmental stages of their offspring.

December 13 Material Copyright, L. Durham

History ofFORENSIC ENTOMOLOGY

First actual case: 1235AD.The Washing Away of Wrongs

Spontaneous generation of maggots in meat was disproved in 1668.

First published account in 1855 in the West (France).

December 13 Material Copyright, L. Durham

Mateu Orfila1787-1853

Father of Modern Toxicology

First systematic observations ofinsect successions on human

cadavers (1848)

History ofFORENSIC ENTOMOLOGY

December 13 Material Copyright, L. Durham

History ofFORENSIC ENTOMOLOGY

Jean Pierre Megnin1828 - 1905

Wrote the founding works ofmodern forensic entomology:

Fauna of Tombs (1887)Fauna of Cadavers (1894)

Wrote a series of articles (1883 – 1896)that made the information accessible

December 13 Material Copyright, L. Durham

Applications

Estimation of PMI

Determination of death location

Criminal Misuse

Abuse of young & elderly

DNA Analysis

Toxicological specimens

Scene characterization

Scope ofFORENSIC ENTOMOLOGY

December 13 Material Copyright, L. Durham

Page 2: Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify FIRST: Eyes, ears, nose, mouth In the event of anti-mortem trauma, flies ... The study

December 13

Material Copyright, Lyndsay S. Durham 2

URBAN

Three Areas inFORENSIC ENTOMOLOGY

STOREDPRODUCT

MEDICOCRIMINAL/MEDICOLEGALDecember 13 Material Copyright, L. Durham December 13

Material Copyright, L. Durham

Adult (30 days)

Egg Mass (1 day)

Larvae (6 days)

Pupae (7 days)

Photo courtesy of JHByrd

Blow Fly Life Cycle

December 13 Material Copyright, L. Durham

Male Gravid Female

December 13 Material Copyright, L. Durham

December 13 Material Copyright, L. Durham December 13 Material Copyright, L. Durham

Page 3: Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify FIRST: Eyes, ears, nose, mouth In the event of anti-mortem trauma, flies ... The study

December 13

Material Copyright, Lyndsay S. Durham 3

December 13 Material Copyright, L. Durham

December 13 Material Copyright, L. Durham December 13 Material Copyright, L. Durham

December 13 Material Copyright, L. Durham December 13 Material Copyright, L. Durham

Page 4: Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify FIRST: Eyes, ears, nose, mouth In the event of anti-mortem trauma, flies ... The study

December 13

Material Copyright, Lyndsay S. Durham 4

FLY SPECIES

2 Main Species Used to Estimate PMI

1. Blow Flies – Calliphoridae2. Flesh Flies - Sarcophagidae

JHBJHB

Forensic Insect Identification CardsDecember 13 Material Copyright, L. Durham

Cochliomyia macellaria

Phaenicia sericata

JHB

Warm, sunny

Warm, sunny

Forensic Insect Identification Cards

Lucilia illustris

Phormia regina

JHB

JHB

Warm, sunny

Cool, shaded

JHB

JHB

JHB

Calliphora vicina

Cool, shade

Chrysomya rufifacies

Tropical, sunny

December 13

Material Copyright, L. Durham

JHB

Sarcophaga haemorrhoidalis

JHB

Musca domestica

Attracted to excrement Summer, shade

Forensic Insect Identification Cards

December 13 Material Copyright, L. Durham

DISPOSITION OF OVIPOSITION

Flies will be attracted to natural orify FIRST:

Eyes, ears, nose, mouth

In the event of anti-mortem trauma, flies will be attracted to wounds and/or the genitalia

December 13 Material Copyright, L. Durham

ENTOMOTOXICOLOGY

How drugs affect the life cycles

of insects

How can insects be used as an

alternative source for toxicological testing

…bugs…

…dr

ugs…

December 13 Material Copyright, L. Durham

Conventional specimens Alternative specimens

The study of the adverse effects of drugs and chemicalson the body and their application to the law.

Brain

LiverKidney

Blood

Vitreous Humor

Bile

Urine

Gastric Contents

Bone Skeletal Muscle

Hair Nails

Insects

FORENSIC TOXICOLOGY

December 13 Material Copyright, L. Durham

Page 5: Mateu Orfila - Ram Pages€¦ · 19/12/2014  · First actual case: 1235AD. ... to natural orify FIRST: Eyes, ears, nose, mouth In the event of anti-mortem trauma, flies ... The study

December 13

Material Copyright, Lyndsay S. Durham 5

The Larvae

Can be ground and analyzed like any postmortem tissue from the body

When collectingsamples for tox analysis, do not placein preservative fluid.

December 13 Material Copyright, L. Durham

The Pupae

Drugs get depositedinto the skin/puparialcasing of the larvae

Can be analyzedin the same manneras hair

December 13 Material Copyright, L. Durham

SUMMARY

Larvae and pupae can be used as atoxicological specimen

The entomologist needs to be informed ifan individual had any drugs on board so he or she knows if and how the time sincedeath estimation should be adjusted

The database for understanding the interactions between drug and insect is currently being developed

December 13 Material Copyright, L. Durham

DNA EXTRACTION

JHB

The gut content of insects can be properlyextracted for DNAanalysis

December 13 Material Copyright, L. Durham

A: green/shiny

B: grey, 3 black stripes

C: dull grey, multiple black stripes