Mass Spectrometry Sample Preparation ToolsTo ensure the best possible analytical results, it remains crucial to have your protein samples in the right chemical environment and at a proper concentration after extraction and collection. Our sample preparation tools provide quick handling and exceptional recovery for small to bigger volumes. Find the best product to suit your needs in our broad portfolio of products for dialysis, desalting, concentration, ion exchange, etc.

Dialysis

Do you need to dialyze large-volume samples and do you want to avoid the hassle of loading your sample into slippery tubing? If so, pre-opened dry Thermo Scientific™ SnakeSkin™ Dialysis Tubing will work for you.Sample volume: 10-100 mL

SnakeSkin Dialysis Tubing

Completely remove salts or other small molecules from your precious low-volume sample using Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Units. The very convenient and safe devices provide maximal sample recovery.Sample volume: 10 μL-2 mL

Slide-A-Lyzer MINI Dialysis Units

If you need to completely remove salts or other small molecules from your sample, Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes are a convenient and safe device and they provide maximum sample recovery.Sample volume: 0,1-30 mL

Slide-A-Lyzer Cassettes

The Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes offer easy sample loading and removal by using a serological pipette. The built-in air chamber provides buoyancy and vertical orientation of the cassette during dialysis. The new Thermo Scientific™ Slide-A-Lyzer™ Dialysis Flasks are designed to process sample volumes of 70 to 250 mL.Sample volume: 0,1-250 mL

Slide-A-LyzerG2 DialysisCassettes and Flasks

Desalting

Concentration

Quickly remove salts and small molecules from your various protein solutions with Thermo Scientific™ Zeba™ Spin Columns and Plates. The easy-to-handle devices provide exceptional protein recovery.Sample volume: 2 μL-4 mL

Zeba Spin Columns

The unique design of Thermo Scientific™ Pierce™ Concentrators provides the best solution for concentrating your protein without losing sample. Concentrators with a polyethersulfone (PES) membrane are also available.Sample volume: 1-20 mL

Concentrators Concentrators PES

Desalt your sample in a cost-efficient way using regenerable Thermo Scientific™ Desalting Columns available with different gels to best suit your protein’s properties.Sample volume: 0,2-1 mL

Polyacrylamide and Dextran Columns

Do you need to pre-fractionate or purify your target protein from a crude biological sample? Thermo Scientific™ Pierce™ Strong Ion Exchange Columns enable rapid protein fractionation and sample preparation based on charge differences. Choose between mini or maxi size and anionic or cationic format of these columns.Sample volume: mini up to 500 μL and maxi up to 20 mL

Strong Ion Exchange Spin Columns

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you have the samples we have the tools

Proper sample preparation for mass spectrometry-based analysis is a critical step in the proteomics workflow. The quality and reproducibility of sample extraction and preparation for downstream analysis significantly impacts the separation and identification capabilities of mass spectrometers.

• C18 Tips, Spin Tips and Spin ColumnsThermo Scientific™ Pierce™ C18 Tips are ready-to-use pipette-tip columns of C18 resins. Thermo Scientific™ Pierce™ C18 Spin Columns are ready-to-use centrifuge columns of porous C18 resins. C18 resins enable fast and efficient capture, concentration, desalting and elution of peptides for MALDI mass spectrometry and other methods. The treatable sample volume varies from 10 to 150 µL according to the type of tips or columns used.

• Graphite Spin Columns Thermo Scientific™ Pierce™ Graphite Spin Columns improve phosphopeptide analysis by efficiently binding hydrophilic peptides and efficiently removing urea, salts and other contaminants before mass spectrometry analysis. The sample volume that can be processed is up to 500 µL per column.

• Abundant Protein Depletion Spin Columns Thermo Scientific™ Pierce™ Abundant Protein Depletion Spin Columns are optimized to decrease the abundant albumin and antibody components during the preparation of human plasma samples for mass spectrometry. Two resin options for top two or top 12 abundant protein depletion are available.

• Detergent Removal Spin Columns and Plates Thermo Scientific™ Pierce™ Detergent Removal Resins efficiently bind to and remove high concentrations of detergents from 10 µL to 1 mL samples with minimal sample loss and are available prepacked into spin columns and 96-well spin filter plates.

• HiPPR Detergent Removal Resin, Columns and Plates Thermo Scientific™ HiPPR Detergent Removal Resins used in spin column or 96-well filter plate formats improve mass spectrometry results by efficiently removing detergents from 25 to 200 µL samples with low protein or peptide concentrations.

Thermo Scientific Sample Clean-up Products

Thermo Scientific Sample Clean-up Products

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G2 Dialysis Cassettes and Flasks

Thermo Scientific™ SnakeSkin™ Dialysis Tubing 3,5K MWCO:

88242 16 mm 35 ft. (10,5 m)

68035 22 mm 35 ft. (10,5 m)

88244 35 mm 35 ft. (10,5 m)

SnakeSkin Dialysis Tubing 7K MWCO:

68700 22 mm 35 ft. (10,5 m)

SnakeSkin Dialysis Tubing 10K MWCO:

88243 16 mm 35 ft. (10,5 m)

68100 22 mm 35 ft. (10,5 m)

88245 35 mm 35 ft. (10,5 m)

SnakeSkin Dialysis Accessories:

68011 Clips 6 clips

Thermo Scientific™ Slide-A-Lyzer™ MINI Dialysis Device, 2K MWCO:

69580 0,1 mL 50 devices

69553 0,1 mL 250 devices

Slide-A-Lyzer MINI Dialysis Device, 3,5K MWCO:

69550 0,1 mL 50 devices

69552 0,1 mL 250 devices

88400 0,5 mL 25 devices

88403 2 mL 25 devices

Slide-A-Lyzer MINI Dialysis Device, 7K MWCO:

69560 0,1 mL 50 devices

69562 0,1 mL 250 devices

Slide-A-Lyzer MINI Dialysis Device, 10K MWCO:

69570 0,1 mL 50 devices

69572 0,1 mL 250 devices

88401 0,5 mL 25 devices

88404 2 mL 25 devices

Slide-A-Lyzer MINI Dialysis Device, 20K MWCO:

69590 0,1 mL 50 devices

69555 0,1 mL 250 devices

88402 0,5 mL 25 devices

88405 2 mL 25 devices

Slide-A-Lyzer MINI Dialysis Device Floats:

69588 25-unit capacity 4 floats

Thermo Scientific™ Protein Concentrators PES:

88512 3K MWCO, 0,5 mL 25 units

88513 10K MWCO, 0,5 mL 25 units

88502 30K MWCO, 0,5 mL 25 units

88503 100K MWCO, 0,5 mL 25 units

Thermo Scientific™ Protein Concentrators, 9K MWCO:

87748 7 mL 10 units

89884A 7 mL 25 units

87749 20 mL 10 units

89885A 20 mL 25 units

Protein Concentrators, 20K MWCO:

87750 7 mL 10 units

89886A 7 mL 25 units

87751 20 mL 10 units

89887A 20 mL 25 units

Protein Concentrators, 150K MWCO:

89920 7 mL 10 units

89922 7 mL 25 units

89921 20 mL 10 units

89923 20 mL 25 units

Thermo Scientific™ Slide-A-Lyzer™ Dialysis Cassettes, 2K MWCO:66205 0,5 mL 10 cassettes

66203 3 mL 10 cassettes

66212 12 mL 8 cassettes

66230 30 mL 6 cassettes

Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 3,5K MWCO:66333 0,5 mL 10 cassettes

66330 3 mL 10 cassettes

66110 12 mL 8 cassettes

66130 30 mL 6 cassettes

Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 7K MWCO:66373 0,5 mL 10 cassettes

66370 3 mL 10 cassettes

66710 12mL 8 cassettes

Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 10K MWCO:66383 0,5 mL 10 cassettes

66384 0,5 mL 5 x 10 cassettes

66380 3 mL 10 cassettes

66381 3 mL 5 x 10 cassettes

66810 12 mL 8 cassettes

66811 12 mL 5 x 8 cassettes

66830 30 mL 6 cassettes

Sterile - Gamma irradiated Slide-A-Lyzer Dialysis Cassettes: check website

Slide-A-Lyzer Dialysis Cassettes, 20K MWCO:66005 0,5 mL 10 cassettes

66003 3 mL 10 cassettes

66012 12 mL 8 cassettes

66030 30 mL 6 cassettes

Thermo Scientific™ Slide-A-Lyzer™ G2 Dialysis Cassettes and Flasks, 2K MWCO:87717 0,5 mL 10 cassettes

87718 3 mL 10 cassettes

87719 15 mL 8 cassettes

87720 30 mL 6 cassettes

87721 70 mL 6 cassettes

87760 250 mL 4 flasks

Slide-A-Lyzer G2 Dialysis Cassettes, 3,5K MWCO:87722 0,5 mL 10 cassettes

87723 3 mL 10 cassettes

87724 15 mL 8 cassettes

87725 30 mL 6 cassettes

87726 70 mL 6 cassettes

87761 230 mL 4 flasks

Slide-A-Lyzer G2 Dialysis Cassettes, 7K MWCO:87727 0,5 mL 10 cassettes

87728 3 mL 10 cassettes

Slide-A-Lyzer G2 Dialysis Cassettes, 10K MWCO:87729 0,5 mL 10 cassettes

87730 3 mL 10 cassettes

87731 15 mL 8 cassettes

87732 30 mL 6 cassettes

87733 70 mL 6 cassettes

87762 250 mL 4 flasks

Sterile - Gamma-irradiated Slide-A-Lyzer G2 Dialysis Cassettes: check website

Slide-A-Lyzer G2 Dialysis Cassettes and Flasks, 20K MWCO:87734 0,5 mL 10 cassettes

87735 3 mL 10 cassettes

87736 15 mL 8 cassettes

87737 30 mL 6 cassettes

87738 70 mL 6 cassettes

87763 250mL 4 flasks

Slide-A-Lyzer Flotation Disk:87759 Slide-A-Lyzer Flotation Disks 6 disks

Thermo Scientific™ Pierce™ Strong Cation Exchange Spin Column:

90008 Mini 24 columns

90009 Maxi 8 columns

Thermo Scientific Pierce™ Strong Anion Exchange Spin Column:

90010 Mini 24 columns

90011 Maxi 8 columns

Dialysis Desalting Concentration Dialysis Tubing and Clips Mini Dialysis Devices Dialysis Cassettes

Strong Ion Exchange Spin Columns

• Convenient – ready-to-use, pre-opened, pre-wetted and pleated tube

• High recovery – greater than 90% protein recovery

• Speed – dialysis is generally completed in four to six hours

• Stability – compatible with a variety of laboratory solutions, including acids, bases, hydrophobic solvents and alcohols

• Three convenient sizes – dialysis devices for 0,1; 0,5 and 2 mL samples fit standard 1,5mL-microcentrifuge, 15 mL and 50 mL-conical tubes, respectively

• Five membrane cut-offs – color coded to easily find the one you want 2K; 3,5K; 7K; 10K and 20K

• Excellent sample recoveries – low-binding plastic and small membrane surface area minimize sample loss compared to filtration and resin systems

• 100% leak-tested – innovative design does not permit “wicking” that can occur in homemade devices

• Time savings – performs desalting of nucleic acids and proteins (>95% recovery) in less than 15 minutes, which improves sample resolution, quantification and conjugation

• Five color-coded membrane cut-offs and volume sizes – select the cassette that best suits your sample volume

• Thickness – 0,9 to 1,2 mL (22,5 to 30 µm)

• High recovery – rectangular cassette design maximizes recovery of entire sample volume via any one of the four corner injection ports

• Fast dialysis – flat cassette chamber with two membranes provides high surface-area to volume ratio that maximizes diffusion rate compared to cylindrical dialysis tubing

• Easy to use – no knots or clamps are needed; just inject sample into cassette and begin dialysis

• Superior design – thoroughly researched and tested to provide fast and consistent dialysis with maximum sample recovery

• Multiple cassette sizes – select the cassette that best suits your sample volume

• Easy loading – pipette-accessible for easy sample loading and retrieval

• Self-floating – integrated air chambers eliminate the need for float buoys (cassettes only)

Thermo Scientific™ Polyacrylamide Desalting Columns 1,8K MWCO:

43426 5 mL 5 columns

Polyacrylamide Desalting Columns 6K MWCO:

43240 5 mL 5 columns

43243 10 mL 5 columns

Polyacrylamide Spin Desalting Columns 7K MWCO:

89849 0,7 mL 25 columns

89862 0,7 mL 50 columns

Thermo Scientific Dextran Desalting Columns 5K MWCO:

43230 5 mL 5 columns

43233 10 mL 5 columns

Polyacrylamide and Dextran Columns

• Chemical stability – stable in water, salt solutions, organic solvents and alkaline or acidic conditions

• Flow rate – approx. 1mL/min (relatively fast compared to other desalting resins)

• Heat stability – autoclavable dry or in solution at a neutral pH for 30 minutes at 120°C without affecting its chromatographic properties

Concentrators

• Rapid protein concentration – achieve greater than 100-fold protein concentration in less than 45 minutes

• Excellent protein recovery – remove buffer and small molecules while retaining more than 90% of proteins larger than 9kDa

• Convenient – two sizes designed to fit 15 mL and 50mL conical tubes; ideal for simple concentration of 1 to 20 mL of sample

• Instrument-compatible – use standard centrifuges with swinging-bucket or fixed-angle rotors; collect and recover sample without invert spinning

• Versatile – the easiest way to perform sample concentration, diafiltration or buffer-exchange of biological samples

Concentrators PES

• Rapid protein concentration – sample con-centration of 10- to 30-fold can be achieved in 10 minutes or less

• Excellent protein recovery – unique design minimizes membrane fouling and provides high speed concentrations, even with particle laden solutions

• Instrument-compatible – use standard microcentrifuge with fixed-angle rotors; collect and recover sample without invert spinning

• Versatile – easy way to perform sample concentration, diafiltration or buffer-exchange of biological samples

• Fast and simple – membrane-based spin format eliminates column packing

• Convenient and expandable – centrifugal format enables convenient processing of multiple samples in parallel

• Robust – membrane adsorber spin columns do not crack or run dry

• Low bed volume – small membrane adsorber bed volumes make working with low buffer volumes possible, leading to concentrated elution fractions

Thermo Scientific™ Zeba™ Spin Desalting Columns, 7K MWCO:

89877 75 µL 25 columns

89878 75 µL 50 columns

89882 0,5 mL 25 columns

89883 0,5 mL 50 columns

89889 2 mL 5 columns

89890 2 mL 25 columns

89891 5 mL 5 columns

89892 5 mL 25 columns

89893 10 mL 5 columns

89894 10 mL 25 columns

Thermo Scientific™ Zeba™ 96-well Spin Desalting Plates, 7K MWCO:

89807 96-well Plates 2 plates

89808 96-well Plates 4 plates

Thermo Scientific™ Zeba™ Chromatography Cartridges, 7K MWCO:

89934 Zeba Desalting Chromatography Cartridges, 1 mL 5 cartridges

89935 Zeba Desalting Chromatography Cartridges, 5 mL 5 cartridges

Zeba Spin Desalting Columns, 40K MWCO:

87764 75 µL 25 columns

87765 75 µL 50 columns

87766 0,5 mL 25 columns

87767 0,5 mL 50 columns

87768 2 mL 5 columns

87769 2 mL 25 columns

87770 5 mL 5 columns

87771 5 mL 25 columns

87772 10 mL 5 columns

87773 10 mL 25 columns

Zeba 96-well Spin Desalting Plates, 40K MWCO:

87774 96-well Plates 2 plates

87775 96-well Plates 4 plates

Zeba Spin Columns

• High recovery – low-binding resin maximizes protein recovery

• Fast – no fraction screening or waiting for protein to emerge by gravity-flow

• Easy to use – no cumbersome column preparation or equilibration

• Flexible – available in spin columns, filter spin plates and cartridges for a range of needs

• Economical – great performance at lower cost than other commercially available cartridges