8/9/2019 Manual Eksperimen Bio 1

1/3

 TITLE: MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE

LEARNING OUTCOME: ANALYSING THE MOVEMENT OF SUBSTANCES ACROSS THE PLASMA

MEMBRANE

“THE SEMI PERMEABILITY OF THE PLASMA MEMBRANE ONLY ALLOWS CERTAIN SUBSTANCES TO

PASS THROUGH IT” 

STUDYING OSMOSIS USING AN OSMOMETER

Material: Benedict sl!tin" starc# s!s$ensin" idine sl!tin" %l!cse sl!tin" Vis&in%

 T!'e" distilled (ater and t#read

A$$arat!s: test t!'es" 'ea&er" B!nsen '!rner" (ire %a!)e" tri$d stand" ca$illar* t!'e"

dr$$er" +, -l -eas!rin% c*linder" -atc#es" test t!'e #lder

A. Y! are re/!ired t cnd!ct an e0$eri-ent !sin% t#e -aterials and a$$arat!s %i1en

2ll(in% t#e $rced!re 'el(3+3 A Vis&in% t!'e 45c-. is sa&ed in distilled (ater 2r 6 t +, -in!tes 'e2re t#e

e0$eri-ent373 Set !$ a$$arat!s as s#(n in t#e dia%ra- 'el(3

83 Mar& t#e initial le1el 2 t#e sl!tin in t#e ca$illar* t!'e393 Lea1e t#e a$$arat!s 2r +, -in!tes363 O'ser1e t#e le1el 2 sl!tin in ca$illar* t!'e3 E0$lain *!r 'ser1atin33 Usin% a dr$$er" ta&e +,-l 2 distilled (ater in t#e 'ea&er and -i0 it (it# +,-l idine

sl!tin3 ;#at can *! 'ser1e< E0$lain *!r 'ser1atin3=3 6-l 2 idine sl!tin is added int t#e 'ea&er and le2t 2r +, -in!tes3 ;#at can *!

'ser1e< E0$lain *!r 'ser1atin3B. Based n *!r 'ser1atins" descri'e t#e -1e-ent 2 s!'stances acrss t#e $las-a

-e-'rane3

8/9/2019 Manual Eksperimen Bio 1

2/3

C. Based n *!r e0$eri-ent res!lts" disc!ss t#e $r'le- 'el(:>!rin% #er 1isit t #er 1illa%e" Pn Salina reali)ed t#at #er -t#er?s $et d!c&s $rd!ce lts 2 

e%%s3 Pn Salina (ants t $rd!ce salted e%% t last ln%er3 S!%%est (a*s 2r Pn3 Salina can

$rd!ce t#e salted e%%3 E0$lain *!r ans(er3

 @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ 

 @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ 

 @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ 

 @@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@@ 

 @@@@ 

STUDYING THE MOVEMENT OF SUBSTANCES ACROSS A SEMI PERMEABLE MEMBRANE

8/9/2019 Manual Eksperimen Bio 1

3/3

Material: Benedict?s sl!tin" + starc# s!s$ensin" idine sl!tin" 8, %l!cse sl!tin"

a Vis&in% t!'e and a cttn t#read

A$$arat!s: test t!'e" 'ea&ers" B!nsen '!rner

A. Y! are re/!ired t cnd!ct an e0$eri-ent !sin% t#e -aterials and a$$arat!s %i1en

2ll(in% t#e $rced!re 'el(3+3 A Vis&in% t!'e is sa&ed in (ater 2r 6 -in!tes3

73 An end 2 t#e Vis&in% t!'e is tied r-l* (it# t#read383 A$$arat!s is set as s#(n 'el(393 Cl!r 2 t#e sl!tin is recrded363 T#e !ter s!r2ace 2 t#e 1is&in% t!'e is rinsed (it# distilled (ater33 A 'ea&er is lled (it# 9,,-l 2 distilled (ater and +6-l 2 idine sl!tin3=3 T#e clr 2 t#e sl!tin is recrded353 Vis&in% t!'e is sa&ed in t#e 'ea&er and le2t 2r 9, -in!tes33 A2ter 9, -in!tes" t#e 1is&in% t!'in% is trans2erred int a dr* 'ea&er3+,3T#e cl!r 2 t#e sl!tins in t#e 1is&in% t!'e and t#e 'ea&er is 'ser1ed and recrded3++3Bt# sl!tins is tested 2r t#e $resence 2 %l!cse !sin% Benedict?s sl!tin3+737-l 2 t#e sl!tin 2r- t#e 'ea&er and 1is&in% t!'e is $!red in 7 se$arate test t!'es

and + -l 2 'enedict?s sl!tin is added int eac# test t!'es3 T#e sl!tins are #eated ina 'ilin% (ater 'at# 2r a'!t 6 -in!tes and t#e c#an%e 2 cl!r is recrded3