Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

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Making Measurements with a Light Microscope

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Transcript of Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Page 1: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Making Measurements

with a Light Microscope

Page 2: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Metric Conversions

• 1 mm = 1000 μm• 1 μm = 1/1000 mm

Page 3: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Instructions

Page 4: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Step 1

• Place a transparent metric ruler under the low power (LP) objective (10X) of a microscope.

Page 5: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Step 2

• Focus the microscope on the scale of the ruler, and measure the diameter of the field of vision in millimeters (mm).

FOV (LP) = 1.2 mm

Page 6: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Step 3

• Convert this measurement to micrometers (μm) by using the following equation:

€

diameter inμ ( ) m LP = diameter in mm X1000 μm

mm

d (LP) = 1.2 X 1000 = 1200 μm

Page 7: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Step 4

• Calculate the diameter in μm of the field of vision under high power (HP) using the following formula:

€

diameter inμ ( ) m HP ( ) . diameter LP x mag of LP objective

. mag of HP objective

d (HP) = (1200 X 10)/40 = 300 μm

Page 8: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Step 5

• To estimate the size of an object seen with a microscope under low power, first estimate what fraction of the diameter of the field of vision that the object occupies (LP). Then multiply the LP diameter you calculated in micrometers (step 3) by that fraction.Size (μm) = fraction X 1200

Page 9: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

€

width = 16 1200 X μ m = 200 μm

€

length = 12 1200 X μ m = 600 μm

Step 5

• Width

• Length

Page 10: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Practice

Problems

Page 11: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

€

width = 14 1200 X μ m = 300 μm

€

width = 12 1200 X μ m = 600 μm

Practice Problem #1

• Width of Red Fiber

• Width of White Fiber

Couch Fibers

100X

Page 12: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

€

height = 23 1200 X μ m = 800 μm

Practice Problem #2

• Height

Water Flea

100X

Page 13: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

Practice Problem #3

Paramecia

100X

• Width

• Length

€

length = 13 1200 X μ m = 400 μm

€

width = 112

1200 X μ m = 100 μm

Page 14: Making Measurements with a Light Microscope. Metric Conversions 1 mm = 1000 μm 1 μm = 1/1000 mm.

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