Magnetic Preparation Kit VI - BIOTECON Diagnostics · 2020-04-25 · Norovirus (Caliciviridae)...
Transcript of Magnetic Preparation Kit VI - BIOTECON Diagnostics · 2020-04-25 · Norovirus (Caliciviridae)...
Applicable for a wide range of matrices:
� Swabs
� Sputum
� Feces
� Whole blood
� Serum
� Tissue
� and many more
Tested at BIOTECON Diagnostics and external labs with:
� SARS-CoV-2 (Coronaviridae)
� Norovirus (Caliciviridae)
� Hepatitis A virus (Picornaviridae)
� PEDV (Coronaviridae)
� ASFV (Asfarviridae)
� Adenovirus (Adenoviridae)
� PRRSV (Arteriviriae)
� Influenza type A virus (Orthomyxoviridae)
� and many more
Safe Virus Detection – BIOTECON Diagnostics –simply builds up trust!
Integrated: No external lysis or heat
treatment
Automated: Reduces hands-on time
Easy storage and shipping: Most
components can be stored at room
temperature
Sensitive and rapid: Excellent RNA / DNA
quality within 45 minutes
Reproducible and precise: Minimizes
human error
Easy: Quick and simple to implement
The Magnetic Preparation Kit VI provides reliable purification of viral nucleic acids (RNA and DNA) from a variety of samples. These include nasopharyngeal and throat
swabs, sputum, feces, serum and tissue.
This newly developed magnetic bead-based isolation kit is designed for automated high-throughput extraction in combination with the RoboPrep® 32, the KingFisherTM Flex or other automation systems. It greatly speeds up the workflow and decreases manual handling, without the need for external lysis or external heat treatment and centrifugation steps. The kit contains all required reagents, including enzyme, buffer and carrier tRNA for the lysis step, as well as magnetic beads to carry out extraction from complex matrices. Using the flexible RoboPrep® 32 allows for the immediate processing of various sample numbers as they arrive in the lab (1-32 per unit at a time).
Automated Extraction of RNA / DNA from Viruses
Magnetic Preparation Kit VI
KD
-MP
KV
I-01
-EN
V02
/202
0
BIOTECON Diagnostics GmbH
Hermannswerder 17 14473 Potsdam Germany
Phone: +49 (0)331-2300-200 Fax: +49 (0)331-2300-299
[email protected] www.bc-diagnostics.com
Magnetic Preparation Kit VI- 480 Reactions
Order No. S 400 20.1 L
Workflow
1. Preparation 2. Sample 3. Extraction
Ext
ract
ion
in 4
5 m
in.
� Washing Buffer I and II � Elution Buffer � Lysis Buffer
� Add 200 μl Sample Material � Press Start � Fully Automated Extraction
The above mentioned instruments are registered trademarks of their respective holders.
Validation
MPK VI was validated with RNA and DNA viruses (enveloped and non-enveloped) from a variety of different samples and species. Results showed excellent sensitivity compared to other kits on the market and high linearity as well as reproducibility even at low virus concentrations (Figure 1 and Figure 2).
Available instruments
D 210 32 RoboPrep® 32 (low to medium throughput, semi-automated)D 220 01 KingFisherTM Flex (medium to high throughput, semi-automated)D 250 00 RoboPrep Fusion® (high throughput, full automated)
Graph shows high linearity and reproducibility even at low virus concentrations
Fig. 1: Amplification plot (PRRSV)
Fig. 2: Standard curve (PRRSV)
For research use only, not intended for diagnostic procedures.
Available PCR-Kits
microproof® SARS-CoV-2 Identification Kitmicroproof® SARS-CoV-2 Screening Kit
Additional PCR-Kits from other manufacturers are available, depending on your region.For further information regarding our SARS-CoV-2 detection kits please contact us.