MACSxpress Cell Isolation from Whole Blood
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MACSxpress® Isolation Kits Fastest cell separation from whole blood
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With the new MACSxpress Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity. Fast: Cell isolation from whole blood in 20 minutes Safe: No centrifugation, less pipetting, minimized aerosol formation Scalable: Sample sizes from 2–30 mL Excellent performance: High purity and recovery Convenient: No density gradient centrifugation Reliable: High reproducibility due to minimal sample manipulation
Transcript of MACSxpress Cell Isolation from Whole Blood
MACSxpress® Isolation Kits
Fastest cell separation from whole blood
Germany/Austria/SwitzerlandMiltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 [email protected]
USA/CanadaMiltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 530 888 8925 [email protected]
Australia Miltenyi Biotec Australia Pty. Ltd. Unit 16A, 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone +61 2 8877 7400 Fax +61 2 9889 5044 [email protected]
BeneluxMiltenyi Biotec B.V. Schipholweg 68 H2316 XE Leiden The Netherlands [email protected] service The Netherlands Phone 0800 4020120 Fax 0800 4020100Customer service Belgium Phone 0800 94016 Fax 0800 99626Customer service Luxembourg Phone 800 24971 Fax 800 24984
ChinaMiltenyi Biotec GmbHShanghai Office Rm. 2309 No. 319 Xianxia Rd. Shanghai 200051, P.R. China Phone +86 21 62351005 Fax +86 21 62350953 [email protected]
FranceMiltenyi Biotec SAS 10 rue Mercoeur 75011 Paris, France Phone +33 1 56 98 16 16 Fax +33 1 56 98 16 17 [email protected]
ItalyMiltenyi Biotec S.r.l. Via Persicetana, 2/D 40012 Calderara di Reno (BO) Italy Phone +39 051 6 460 411 Fax +39 051 6 460 499 [email protected]
JapanMiltenyi Biotec K.K. Nittsu-Eitai Building 5F 16-10 Fuyuki, Koto-ku, Tokyo 135-0041, Japan Phone +81 3 5646 8910 Fax +81 3 5646 8911 [email protected]
Nordics and BalticsMiltenyi Biotec Norden ABScheelevägen 17223 70 [email protected] service Sweden Phone 0200-111 800 Fax 046-280 72 99 Customer service Denmark Phone 80 20 30 10 Fax +46 46 280 72 99 Customer service Norway, Finland, Iceland, and Baltic countries Phone +46 46 280 72 80 Fax +46 46 280 72 99
SingaporeMiltenyi Biotec Asia Pacific Pte Ltd. 100 Beach Road #28-06 to 28-08 Shaw Tower Singapore 189702 Phone +65 6238 8183 Fax +65 6238 0302 [email protected]
South KoreaMiltenyi Biotec KoreaPetra building659 Yeoksam-dongGangnam-guSeoul, South Korea Phone +82 2 555 1988 Fax +82 2 555 8890 [email protected]
SpainMiltenyi Biotec S.L. C/Luis Buñuel 2 Ciudad de la Imagen 28223 Pozuelo de Alarcón (Madrid) Spain Phone +34 91 512 12 90 Fax +34 91 512 12 91 [email protected]
United Kingdom Miltenyi Biotec Ltd. Almac House, Church Lane Bisley, Surrey GU24 9DR, UK Phone +44 1483 799 800 Fax +44 1483 799 811 [email protected]
www.miltenyibiotec.com
130-
100-
072
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSmix, MACSQuant and MACSxpress are registered trademarks or trademarks of Miltenyi Biotec GmbH. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2013 Miltenyi Biotec GmbH. All rights reserved.
MACSxpress® Isolation KitsFast large-scale isolation from whole blood
MACSxpress® Cell Isolation at a glanceMACSxpress is the only technology which enables isolation of untouched cells from whole blood without density gradient centrifugation.
Fast: Cell isolation from whole blood in 20 minutes
Safe: No centrifugation, less pipetting, minimized aerosol formation
Scalable: Sample sizes from 2–30 mL
Excellent performance: High purity and recovery
Convenient: No density gradient centrifugation
Reliable: High reproducibility due to minimal sample manipulation
Figure 1: The MACSxpress Isolation Kits, human together with the MACSxpress Separator enables isolation of untouched cells directly from whole blood.
How it worksWith the new MACSxpress Technology, untouched human leukocyte subsets can be isolated from up to 30 mL of anticoagulated whole blood. Non-target cells are removed by immunomagnetic depletion using MACSxpress Beads. Simultaneously, erythrocytes are sedimented, yielding untouched target cells of high purity.
Add reconstituted MACSxpress Cell Isolation Cocktail to whole blood.
Incubate sample for 5 minutes at room temperature using the MACSmixTM Tube Rotator.
Place open tube in the magnetic field of the MACSxpress Separator for 15 minutes.
Collect the supernatant which contains the unlabeled target cells.
Figure 2: Principle of MACSxpress Technology. Isolate cells directly from whole blood in 20 minutes.
A wide range of benefits Combining speed, safety, and scalability
Excellent performanceWith MACSxpress Technology, purities up to 97% and recoveries of up to 80% can be achieved.
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Figure 4: Reliable performance with MACSxpress Isolation Kits. B cells, T cells, and NK cells were isolated from 5 different donors using the MACSxpress Isolation Kits (Order no. 130-098-190, 130-098-193, and 130-098-185, respectively). Purities and recoveries were assessed by flow cytometry using the MACSQuant® Analyzer.
Product information
MACSxpress Kits Order no.
MACSxpress B Cell Isolation Kit, human 130-098-190
MACSxpress Naive B Cell Isolation Kit, human 130-098-186
MACSxpress NK Cell Isolation Kit, human 130-098-185
MACSxpress Pan T Cell Isolation Kit, human 130-098-193
MACSxpress CD4 T Cell Isolation Kit, human 130-098-195
MACSxpress CD8 T Cell Isolation Kit, human 130-098-194
MACSxpress Separator 130-098-308
MACSmix Tube Rotator 130-090-753
For more information visit www.macsxpress.com
Go from whole blood to pure cells in 20 minutes
With the fastest technology available, why wait for long centrifugation runs, when you could isolate your cells directly from whole blood?
Processing time is drastically reduced – making it better for the cells and better for you.
SeparationLabeling
35 min
Centrifugation without brake
Density gradient centrifugation
Cell isolation with MACSxpress® Technology
40 min
Labeling Separation
Competitor technology
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Time (min)
Figure 3: MACSxpress Cell Isolation versus density gradient centrifugation. A leukocyte subset isolation with MACSxpress takes less time than a preparative density gradient centrifugation.
Maximize safetyIf you work with untested blood samples, your safety has to come first. Aerosol development can be a significant hazard during processing of blood samples. With MACSxpress Cell Isolation, there is no centrifugation and pipetting steps are minimized.
MACSxpress Technology means less handling of hazardous material so the opportunity for aerosol is reduced.
Scalable to fit your needsMultiple blood samples with volumes between 2 and 30 mL can be processed in parallel. No more splitting large-volume samples – saving you even more time.
Germany/Austria/SwitzerlandMiltenyi Biotec GmbH Friedrich-Ebert-Straße 68 51429 Bergisch Gladbach Germany Phone +49 2204 8306-0 Fax +49 2204 85197 [email protected]
USA/CanadaMiltenyi Biotec Inc. 2303 Lindbergh Street Auburn, CA 95602, USA Phone 800 FOR MACS Phone +1 530 888 8871 Fax +1 530 888 8925 [email protected]
Australia Miltenyi Biotec Australia Pty. Ltd. Unit 16A, 2 Eden Park Drive Macquarie Park NSW 2113 Australia Phone +61 2 8877 7400 Fax +61 2 9889 5044 [email protected]
BeneluxMiltenyi Biotec B.V. Schipholweg 68 H2316 XE Leiden The Netherlands [email protected] service The Netherlands Phone 0800 4020120 Fax 0800 4020100Customer service Belgium Phone 0800 94016 Fax 0800 99626Customer service Luxembourg Phone 800 24971 Fax 800 24984
ChinaMiltenyi Biotec GmbHShanghai Office Rm. 2309 No. 319 Xianxia Rd. Shanghai 200051, P.R. China Phone +86 21 62351005 Fax +86 21 62350953 [email protected]
FranceMiltenyi Biotec SAS 10 rue Mercoeur 75011 Paris, France Phone +33 1 56 98 16 16 Fax +33 1 56 98 16 17 [email protected]
ItalyMiltenyi Biotec S.r.l. Via Persicetana, 2/D 40012 Calderara di Reno (BO) Italy Phone +39 051 6 460 411 Fax +39 051 6 460 499 [email protected]
JapanMiltenyi Biotec K.K. Nittsu-Eitai Building 5F 16-10 Fuyuki, Koto-ku, Tokyo 135-0041, Japan Phone +81 3 5646 8910 Fax +81 3 5646 8911 [email protected]
Nordics and BalticsMiltenyi Biotec Norden ABScheelevägen 17223 70 [email protected] service Sweden Phone 0200-111 800 Fax 046-280 72 99 Customer service Denmark Phone 80 20 30 10 Fax +46 46 280 72 99 Customer service Norway, Finland, Iceland, and Baltic countries Phone +46 46 280 72 80 Fax +46 46 280 72 99
SingaporeMiltenyi Biotec Asia Pacific Pte Ltd. 100 Beach Road #28-06 to 28-08 Shaw Tower Singapore 189702 Phone +65 6238 8183 Fax +65 6238 0302 [email protected]
South KoreaMiltenyi Biotec KoreaPetra building659 Yeoksam-dongGangnam-guSeoul, South Korea Phone +82 2 555 1988 Fax +82 2 555 8890 [email protected]
SpainMiltenyi Biotec S.L. C/Luis Buñuel 2 Ciudad de la Imagen 28223 Pozuelo de Alarcón (Madrid) Spain Phone +34 91 512 12 90 Fax +34 91 512 12 91 [email protected]
United Kingdom Miltenyi Biotec Ltd. Almac House, Church Lane Bisley, Surrey GU24 9DR, UK Phone +44 1483 799 800 Fax +44 1483 799 811 [email protected]
www.miltenyibiotec.com
130-
100-
072
Miltenyi Biotec provides products and services worldwide. Visit www.miltenyibiotec.com/local to find your nearest Miltenyi Biotec contact.
Unless otherwise specifically indicated, Miltenyi Biotec products and services are for research use only and not for therapeutic or diagnostic use. MACS, the MACS logo, MACSmix, MACSQuant and MACSxpress are registered trademarks or trademarks of Miltenyi Biotec GmbH. All other trademarks mentioned in this document are the property of their respective owners and are used for identification purposes only. Copyright © 2013 Miltenyi Biotec GmbH. All rights reserved.
