M. Strokin et al., 2003 DHA – AA in astrocytes
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Fig. 2. Comparison of the effects of different concentrations of DHA on astrocyte morphology. Ten day old astrocytes grown in DMEM/ F12 were treated with 25, 50, and 100 mM DHA in 0.1% ethanol and 1% FBS for an additional 2 days (B, C, D, respectively). A shows cells grown under serum-deficient conditions in DMEM/F12, and E shows cells cultured in 10% normal serum throughout the culture. Cells were immunofluorescence stained with glial fibrillary acidic protein (GFAP) and observed with a confocal microscope to ascertain their morphology. Effects of DHA on the induction of protein kinase A (PKA) activity by isoproterenol (ISP) in astrocyte cultures. Effects of ISP on the morphological differentiation of astrocytes supplemented with DHA. Ten day old erebral astrocyte cultures were supplemented with 100 mM DHA and 1% FBS for an additional 2 days. Cells were Docosahexaenoic acid facilitates cell maturation and b- adrenergic transmission in astrocytes Joardar et al., 2006; Journal of Lipid Research Vol 47, 2006
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Docosahexaenoic acid facilitates cell maturation and b-adrenergic transmission in astrocytes Joardar et al., 2006; Journal of Lipid Research Vol 47, 2006. - PowerPoint PPT Presentation
Transcript of M. Strokin et al., 2003 DHA – AA in astrocytes
Fig. 2. Comparison of the effects of different concentrations of DHA on astrocyte morphology. Ten day old astrocytes grown in DMEM/ F12 were treated with 25, 50, and 100 mM DHA in 0.1% ethanol and 1% FBS for an additional 2 days (B, C, D, respectively). A shows cells grown under serum-deficient conditions in DMEM/F12, and E shows cells cultured in 10% normal serum throughout the culture. Cells were immunofluorescence stained with glial fibrillary acidic protein (GFAP) and observed with a confocal microscope to ascertain their morphology.
Effects of DHA on the induction of protein kinase A (PKA) activity by isoproterenol (ISP) in astrocyte cultures.
Effects of ISP on the morphological differentiation of astrocytes supplemented with DHA. Ten day old erebral astrocyte cultures were supplemented with 100 mM DHA and 1% FBS for an additional 2 days. Cells were treated without (A) or with 1 mM ISP (B) for 48 h.
Docosahexaenoic acid facilitates cell maturation and b-adrenergic transmission in astrocytes Joardar et al., 2006; Journal of Lipid Research Vol 47, 2006
Release of DHA (a,c) and AA (b,d) from rat astrocytes stimulated with ATP (a,b), concentration dependence, (c,d) time dependence.
Release of DHA (a) and AA (b) from rat astrocytes stimulated with different agents. Cells were labeled with [14C]docosahexaenoic acid or [3 H]arachidonic acid in DMEM containing 10% fetal calf serum for 24 h, washed twice with HBS, and fresh DMEM was added. Then 20 min later, agents (ATP, 20 mm; thrombin, 0.1Uml1; bradykinin, 1 mm; and glutamate, 100 mm) were added at 371C for 15 min and then radioactivity in the medium was measured
M. Strokin et al., 2003 DHA – AA in astrocytes
Fig. 2. Effect of 5-HT and 5-HT2 receptor agonist and on the release ofw3 x 14 H and C from C6 glioma cells. Confluent cultures of C6 glioma cellsprelabeled with w3HxAA closed bars. and w14CxDHA hatched bars. 10mM, 0.5 mCirwell, 24 h. were washed and incubated in the presence ofthe indicated concentrations of serotonin 5-HT. or DOI for 10 min.
M.C. Garcia, H.-Y. KimrBrain Research 768 (1997) 43–48
Maternal Parity and Diet (n-3) PolyunsaturatedFatty Acid Concentration Influence Accretion of Brain Phospholipid Docosahexaenoic Acid in Developing RatsMarlies K. Ozias, Susan E. Carlson, and Beth Levant, 2006*
