LudgerPureTM 2AB Labeled NA4 Glycan€¦ · Detector: Jasco FP920 Excitation wavelength: 360 nm...

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Certificate of Analysis LudgerPure TM 2AB Labeled NA4 Glycan Cat. # : CAB-NA4-01 Lot # : A4BJ-01 Size : approx. 100 pmol Purity: > 90% pure as assessed by HPLC (see Fig 1) 5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0 -50 200 400 600 LudgerSepN1-A51Q-01-CAB-NA4-01-A4BJ-01 Fluorescence mV min 10.49 12.74 37.87 42.92 45.52 Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA4 glycan (Cat. #: CAB-NA4-01, Lot No. A4BJ-01). HPLC Running Conditions Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4 Gradient: 0-75 min - 35-65 % B 75-80 min - 65-100 % B 80-83 min - 100 % B 83-85 min - 100-35 % B 85-115 min - 35 % B Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Transcript of LudgerPureTM 2AB Labeled NA4 Glycan€¦ · Detector: Jasco FP920 Excitation wavelength: 360 nm...

Certificate of Analysis

LudgerPureTM 2AB Labeled NA4 Glycan

Cat. # : CAB-NA4-01 Lot # : A4BJ-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

200

400

600 LudgerSepN1-A51Q-01-CAB-NA4-01-A4BJ-01 FluorescencemV

min

10.4

9

12.7

4

37.8

7

42.9

2

45.5

2

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA4 glycan (Cat. #: CAB-NA4-01, Lot No. A4BJ-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NGA4 Glycan

Cat. # : CAB-NGA4-01 Lot # : A51E-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

200

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600 LudgerSepN1-A51Q-01-CAB-NGA4-01-A51E-01 FluorescencemV

min

7.76

10.4

6

26.0

3

27.7

528

.97

30.5

7

37.2

4

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA4 glycan (Cat. #: CAB-NGA4-01, Lot No. A51E-

01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled A3 Glycan

Cat. # : CAB-A3-01 Lot # : A4AQ-02 Size : approx. 100 pmol

Purity: >90% by hplc (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

100

200

300 LudgerSepN1-A51Q-01-CAB-A3-01-A4AQ-02 FluorescencemV

min

7.77 40

.67

40.7

7 42.3

3

44.2

5

46.0

247

.47

49.1

9

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A3 glycan (Cat. #: CAB-A3-01, Lot No. A4AQ-02).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NA3 Glycan

Cat. # : CAB-NA3-01 Lot # : A4AT-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

450 LudgerSepN1-A51Q-01-CAB-NA3-01-A4AT-01 FluorescencemV

min

33.3

434

.76

38.0

0

42.9

2

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA3 glycan (Cat. #: CAB-NA3-01, Lot No. A4AT-

01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NGA3 Glycan

Cat. # : CAB-NGA3-01 Lot # : A51H-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

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450 LudgerSepN1-A51Q-01-CAB-NGA3-01-A51H-01 FluorescencemV

min

7.77 10

.48

25.8

826

.61

27.6

6

31.0

6

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA3 glycan (Cat. #: CAB-NGA3-01, Lot No.

A51H-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NGA3 Glycan

Cat. # : CAB-NGA3-01 Lot # : A51H-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

450 LudgerSepN1-A51Q-01-CAB-NGA3-01-A51H-01 FluorescencemV

min

7.77 10

.48

25.8

826

.61

27.6

6

31.0

6

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA3 glycan (Cat. #: CAB-NGA3-01, Lot No.

A51H-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled A2F Glycan

Cat. # : CAB-A2F-01 Lot # : A4AR-02 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-100

250

500

900 LudgerSepN1-A51Q-01-CAB-A2F-01-A4AR-02 FluorescencemV

min

39.3

6

43.3

944

.96

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A2F glycan (Cat. #: CAB-A2F-01, Lot No. A4AR-02).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled A1F Glycan

Cat. # : CAB-A1F-01 Lot # : A4AJ-02 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

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600 LudgerSep N1-A4AP-01-CAB-A1F-01-A4AJ-02 FluorescencemV

min

35.1

036

.03

37.5

238

.97

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A1F glycan (Cat. #: CAB-A1F-01, Lot No. A4AJ-02).

HPLC Running Condition

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NA2F Glycan

Cat. # : CAB-NA2F-01 Lot # : A531-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

200

400

600 LudgerSepN1-A52A-01-CAB-NA2F-01-A531-01 FluorescencemV

min

7.81

9.16

10.6

1

31.2

7

32.9

1

34.5

2

37.7

639

.02

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA2F glycan (Cat. #: CAB-NA2F-01, Lot No. A531-

01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NGA2F Glycan

Cat. # : CAB-NGA2F-01 Lot # : A51C-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

375

500 LudgerSepN1-A51Q-01-CAB-NGA2F-01-A51C-01 FluorescencemV

min

6.82 7.78

10.4

4

25.9

6

27.8

3

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NGA2F glycan (Cat. #: CAB-NGA2F-01, Lot No.

A51C-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB labeled A1 Glycan

Cat. # : CAB-A1-01 Lot # : A4AR-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-100

250

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900 LudgerSepN1-A51Q-01-CAB-A1-01-A4AR-01 FluorescencemV

min

33.0

8

36.0

7

37.8

6

39.6

4

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled A1 glycan (Cat. #: CAB-A1-01, Lot No. A4AR-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NA2 Glycan

Cat. # : CAB-NA2-01 Lot # : A4BP-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1).

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

375

500 LudgerSepN1-A51Q-01-CAB-NA2-01-A4BP-01 FluorescencemV

min

7.78

9.99

33.4

1

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled NA2 glycan (Cat. #: CAB-NA2-01, Lot No. A4BP-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled NGA2 Glycan

Cat. # : CAB-NGA2-01 Lot # : A4C2-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

450 LudgerSepN1-A51Q-01-CAB-NGA2-01-A4C2-01 FluorescencemV

min

7.76

8.27

10.4

4

24.7

725

.88

Figure 1 LudgerSepN1 HPLC profile of 2AB labeled NGA2 glycan (Cat. #: CAB-NGA2-01, Lot No. A4C2-

01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm.

Certificate of Analysis

LudgerPureTM 2AB Labeled MAN-9 Glycan

Cat. # : CAB-MAN9-01 Lot # : A4BO-05 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

100

200

300

400 LudgerSepN1-A4B9-01-CAB-MAN9-01-A4BO-05 FluorescencemV

min

7.39

42.5

2

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-9 glycan (Cat. #: CAB-MAN9-01, Lot No.

A4BO-05).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled MAN-8 Glycan

Cat. # : CAB-MAN8-01 Lot # : A532-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-30

0

25

50

80 LudgerSepN1-A522-01-CAB-Man8-01-A532-01 FluorescencemV

min

7.73

40.8

041

.56

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-8 glycan (Cat. #: CAB-MAN8-01, Lot No.

A532-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled MAN-7 Glycan

Cat. # : CAB-MAN7-01 Lot # : A4BN-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

100

200

300 LudgerSepN1-A4B9-01-CAB-MAN7-01-A4BN-01 FluorescencemV

min

8.01 33

.24

36.6

8

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-7 glycan (Cat. #: CAB-MAN7-01, Lot No.

A4BN-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled MAN-6 Glycan

Cat. # : CAB-MAN6-01 Lot # : A4BM-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-20

50

100

150

200 LudgerSepN1-A4B9-01-CAB-MAN6-01-A4BM-01 FluorescencemV

min

33.1

7

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN6 glycan (Cat. #: CAB-MAN6-01, Lot No.

A4BM-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled MAN-5 Glycan

Cat. # : CAB-MAN5-01 Lot # : A4BQ-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

200

400

600 LudgerSepN1-A51Q-01-CAB-MAN5-01-A4BQ-01 FluorescencemV

min

7.75

9.91

25.7

6

29.9

9

33.9

0

36.8

9

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled MAN-5 glycan (Cat. #: CAB-MAN5-01, Lot No.

A4BQ-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm

Certificate of Analysis

LudgerPureTM 2AB Labeled Hybrid Glycan

Cat. # : CAB-HYBRID-01 Lot # : A51I-01 Size : approx. 100 pmol

Purity: > 90% pure as assessed by HPLC (see Fig 1)

5.0 10.0 15.0 20.0 25.0 30.0 35.0 40.0 45.0 50.0 55.0 60.0 65.0 70.0 75.0 80.0 85.0 90.0-50

125

250

375

500 LudgerSepN1-A51Q-01-CAB-HYBRID-01-A51I-01 FluorescencemV

min

6.78 7.77

10.4

6

33.3

0

Figure 1 : LudgerSepN1 HPLC profile of 2AB labeled HYBRID glycan (Cat. #: CAB-HYBRID-01, Lot No.

A51I-01).

HPLC Running Conditions

Column: LudgerSep N1 Amide (Cat. #: LS-N1-4.6x250) Flow: 0.4 ml/min Temperature: 40 °C

Solvent A: 100 % acetonitrile Solvent B: 250 mM ammonium formate pH 4.4

Gradient: 0-75 min - 35-65 % B

75-80 min - 65-100 % B

80-83 min - 100 % B

83-85 min - 100-35 % B

85-115 min - 35 % B

Detector: Jasco FP920 Excitation wavelength: 360 nm Emission wavelength: 425 nm