LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013

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Transcript of LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013

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LOGO Twinkle Medicine Hunter Of Cancer LZU-China iGEM Team 2013 Slide 2 LOGO Content 1 2 3 4 Introduction Experimental Human Practice Results & Discussion Slide 3 LOGO Background: About Cancer Estimated Number of Persons Alive in the U.S. Diagnosed with Cancer in 2006 (N = 11.4 M survivors) Estimated Number of Female Cancer Survivors in the U.S. in 2006 (N = 6.2 M survivors) Chap.1 Chap.2 Chap.3 Chap.4 Estimated Number of Persons Alive in the U.S. Diagnosed with Cancer on January 1, 2006 by Time From Diagnosis and Gender (Invasive/1st Primary Cases Only, N = 11.4 M survivors Slide 4 LOGO Background: About Cancer Chap.1 Chap.2 Chap.3 Chap.4 What cause cancer? Cancer cells come from normal cells, what changed? Gene mutation Slide 5 LOGO Chap.1 Chap.2 Chap.3 Chap.4 Background: Overview of NF-B classical signaling pathway Activated NFkB were found in almost 70% cancer cells. NFkB signaling pathway has become a variety of malignant tumor therapy and drug discovery is an important target. Slide 6 LOGO Chap.1 Chap.2 Chap.3 Chap.4 Background: Overview of NF-B classical signaling pathway NF-B Signaling Pathway FADD IB- NF-B p65/50 NF-B p65/50 IB- cIAP1/2 Bcl-xL/2 Survivin ICAM-1 Cyclin-D1 VEGF Gene expression Cytoplasm PKC Nucleu IKKs PMA TNF- IL-1 TNFR IL-1R MEKKS TRADD RIP TRAFs TRAF6 TAK1 MyD88 IRAK P P P IB- Ub P Proteasome Slide 7 LOGO Background: Negative feedback loop of NF-B Chap.1 Chap.2 Chap.3 Chap.4 Negative feedback loop Slide 8 LOGO Background: Goal How to Change the situation Chap.1 Chap.2 Chap.3 Chap.4 Traditional : LOW accuracy Mice live short/ Cant take long-term detection ? Slide 9 LOGO Experimental: Device Promoter: 4 copies of B Binding sequence Chap.1 Chap.2 Chap.3 Chap.4 IB+GFP Terminator Pref Suff pSB1C3 Slide 10 LOGO Experimental: Design Cancer Why IB exists? Chap.1 Chap.2 Chap.3 Chap.4 NF-B signaling pathway feedback loop dynamis To last a long term detection Sacrifice less mice Slide 11 LOGO Experimental: Device Promoter: 4 copies of B Binding sequence Chap.1 Chap.2 Chap.3 Chap.4 IB+GFP Terminator Pref Suff pSB1C3 Slide 12 LOGO Background: Goal Chap.1 Chap.2 Chap.3 Chap.4 NF-B signal is inhibited. Oscillation represents drugs duration time. Fluorescent light Time Slide 13 LOGO Experimental: Results & Discussion: Results Chap.1 Chap.2 Chap.3 Chap.4 DU-145 IB GAPDH shRNA- IB - + Slide 14 LOGO Experimental: Results & Discussion: Results Chap.1 Chap.2 Chap.3 Chap.4 DU145 Cell DU-145 IB GAPDH Slide 15 LOGO Experimental: Results & Discussion: Future Goals 1. Making our drug screening system work better. 1. Improve specifity: building the fine- adjusted in other cells. 2. Shorten twinkle cycle-time: show some features in pharmacokinetics of drugs. 3. Achieve semiquantitative measure: detect the light intensity of GFP and make a standard curve. Chap.1 Chap.2 Chap.3 Chap.4 Slide 16 LOGO Experimental: Results & Discussion: Application 1. Making our drug screening system work better. 1. How to do: inject DU-145 in which have a moderate expression level of IB. 2. Long-term drug evaluation system: tumor grow slowly, mice survive longer. 3. Sensitive system: observe twinkle instead o measuring the size of tumor. Chap.1 Chap.2 Chap.3 Chap.4 Slide 17 LOGO Experimental: Chap.1 Chap.2 Chap.3 Chap.4 Human Practice Our team had a interchange with Peking iGEM team and BIT iGEM team in August. We shared our own idea to each other, and offered advice for the problem. Our Teams member Li Xiaomeng had been honoured to visit the BIT iGEM 2013 Teams lab. And help them to characterize and construct a part of Cr sensor. Slide 18 LOGO Experimental: Results & Discus Chap.1 Chap.2 Chap.3 Chap.4 Human Practice We held two promotion meetings concerning iGEM for all students in our school and Lanzhou No.1 Meddle School successfully. Meanwhile, the opening day of iGEM lab has been excellently work out with the amazing enthusiasm in this summer vacation. Slide 19 LOGO Acknowledgements Ph.D Jinbo Yang, Huyuan Feng & Their Labs Institute of Cancer Biology and Drug Screening of Lanzhou University School of Life Science of Lanzhou University Chap.1 Chap.2 Chap.3 Chap.4 Slide 20 LOGO THANKS!