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Logical In-Vial Dilution with Ultrafiltration for Anion

Analysis

IC Application Work AW US6-0149-012011

Presented by Carlos Bazán

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Overview

• Dosino technology provides a highly efficient and accurate method for performing dilutions in-line; saving customers time, money, and reducing human error

• Previous configurations required external mixing with added components and added $$

• The In-Vial Dilution configuration can perform dilutions in an empty sample vial and utilizes the Dosino to mix the diluted sample

• Intelligent re-dilution of samples above the calibration range is possible

• Next-Vial Dilution allows the next sample in the series to be diluted while the current sample is still running, maintaining high sample through-put

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Components needed• 881, 882, or 850 IC

• 858 Sample Processor with peristaltic pump (2.858.0020)

• 800 Dosino with 10 mL Dosing Unit

• IC Equipment for Ultrafiltration (6.5330.010)

• T-Connector 3 X UNF 10-32 (6.2744.290; diverts flow for dilution or filtration)

• In-line check valve (6.2744.190; prevents Dosino from pulling from ultrafiltration cell waste)

• 10 mL Transfer Capillary (6.1562.130)

• Wash station for IC (6.2841.100)

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Basic function• Sample is aspirated into the transfer capillary by the Dosino. The in-

line check valve prevents UF cell waste from being aspirated.

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Basic function• The aspirated sample and a quantity of UHP H2O to complete the

dilution are dispensed into an empty sample vial.

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Basic function• The diluted sample is aspirated and dosed several times to mix it

completely.

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Basic function• The peristaltic pump moves the diluted sample through the

ultrafiltration cell and to the IC.

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MagIC Net Sample Table

Injections= 1 for normal analysis, 2 when an intelligent re-dilution check should be performed

Dilution= the Manual Dilution Factor for hand-dilutions made

Value 1= the Dosino Dilution Factor; the dilution the instrument will perform

Value 2= the Dilution Vial; vial position the dilution is performed in

Value 3= the Intelligent Dilution Vial; vial position for intelligent re-dilution when needed

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Features: Calibration• Calibration from one stock standard; levels made by variable

dilution• Makes standard prep easier, saves time, reduces human error

Dilution Factor (Value 1)

200 100 50 20 10

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Features: Next-Vial Dilution• Prepares the dilution of the next sample in the series while the

previous sample is still running• Reduces the overall dilution time needed for a sample series;

very valuable for labs needing high through-put• Can be used with intelligent re-dilution; during the re-dilution run

the next sample is prepared• Uses the SD.NEXT values in the program to access the POSITION,

VALUE1 and VALUE2 of the next line in the sample table

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Features: Multi-component Intelligent Re-dilution• Software can evaluate which out of the entire list of

component ions is most above the calibration range • Re-dilutes samples accordingly

If this is not an intelligent dilution run (Injection=1),or the Area of Fluoride =0,then Autodilution Factor for Fluoride=0.

If it is an intelligent dilution run (Injection=2) then Autodilution Factor for Fluoride = (Concentration of Fluoride in current run / (Concentration of Fluoride in the highest standard X the Manual Dilution Factor )) X the Dosino Dilution Factor

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Features: Multi-component Intelligent Re-dilution

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Performance: Calibration Linearity

• Low calibration RSD

• Linear across a broad calibration range

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Performance: Dilution Accuracy

• 99%+ Accuracy compared to a 1:10 hand-dilution• 96%+ Accuracy compared to a 1:100 hand-dilution

(1:100 is maximum advisable Dosino-prepared dilution with 10 mL Dosing Unit)

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Performance: Reproducibility

• Approximately 1.5% RSD on 6 replicate injections of 1:100 dilution (with lower RSD at lesser dilution factors).

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Performance: Intelligent Re-dilution• System can re-dilute samples based on which ion is most above the

calibration range; can compare all analytes in component table

Highest standard

Samples

Fluoride Cl N02 as N Br NO2 as N P04 as P SO4

Std 5 3.972 ppm 100.463 ppm 1.507 ppm 1.977 ppm 10.072 ppm 9.699 ppm 98.899 ppm

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Market Potential

• A large percentage of environmental and QC laboratories must routinely perform sample dilutions

• User proficiency is going down, so reliable in-line, automated methods for dilution and standard preparation are highly valuable

• Automatic re-dilution of samples above the calibration range saves time, especially on samples with 48 hr. hold-time

• LOW COST OF THE SYSTEM MEANS WE SHOULD SELL A LOT OF THESE TO A LOT OF USERS!

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Acknowledgements

• Jay Kim for invaluable contributions to programming of the intelligent dilution and next-vial dilution components of the method

• Dr. Shibu Paul for the initial development request and continuing drive to perfect the method

• Dr. Hari Narayanan for advice and guidance

• Larry Tucker for suggestions on sample flow path plumbing and wisdom on Dosino capabilities

• Dr. Stuart Procter for method testing during technical training sessions

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Questions?

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