Blue Earth

LIVER FUNCTION TESTSDr. Vijay Marakala , M.B.B.S, M.D.BIOCHEMISTRYSIMS & RC

1

Functions of liverClassification of LFT

FUNCTIONS OF LIVER

CLASSIFICATION OF LIVER FUNCTION TESTS

According to function of liverClassification of LFTTests based on synthetic function

Tests based on excretory function

Tests based on detoxification function

Tests based on metabolic function

Determination of serum enzymes

I IIIII IV V

I. Tests based on excretory function

II. Tests b/o detoxification function

III. Tests b/o synthetic function

Tests related to CARBOHYDRATE metabolismTests related to LIPID metabolismTests related to PROTEIN metabolism

IV. Tests b/o metabolic function

Galactose tolerance testSerum cholesterolSerum proteinsAminoaciduria

V. Enzymes in diagnosis of liver disease

I. Tests based on excretory function

I. Tests based on excretory function

I. Tests based on excretory function1.Serum bilirubin

Serum bilirubin estimation is based on van den Bergs diazo reaction.Diazo reagent consists of diazotized sulfanic acidDetermination of Serum Bilirubin

I. Tests based on excretory function

I. Tests based on excretory function

I. Tests based on excretory function

Laboratory results S BilirubinU UrobilinogenU BilirubinF UrobilinogenConjugated UnconjugatedNormal 0.1-0.4mg/dl0.2-0.7mg/dl0.4mg/dayAbsent40-280mg/dayPrehepatic NormalIncreased IncreasedAbsentIncreased Hepatic IncreasedIncreasedN/Decreased Present Decreased Posthepatic IncreasedNormalAbsent Present Trace to absent

I. Tests based on excretory function

Tests b/o detoxification function

Liver is the main source of synthesis of Plasma proteins - Albumin - Globulin(except )Blood clotting factors - Prothrombin and factors V, VII and X. Tests b/o synthetic function

Determination of serum albumin and globulinAlbumin/globulin ratio= 1.2:1 to2.5:1

Determination of Prothrombin time

Tests related to CARBOHYDRATE metabolismTests related to LIPID metabolismTests related to PROTEIN metabolism

Tests b/o metabolic function

Galactose tolerance testSerum cholesterolSerum proteinsAminoaciduria

In liver cell injury, damage to the membrane of cells and organelles allows intracellular enzymes to leak into the blood.Where their elevated concentrations can be measuredEnzymes in diagnosis of liver disease

Serum transaminasesAST or SGOT=