LIN YECHUN 2015 - CORESTA · LIN YECHUN 2015.09 2015_TSRC106_LinYechun.pdf TSRC2015(69) - Document...

LIN YECHUN2015.09

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CONTENTS

Background

Changed Micro-environment

Seedlings Growth and Photosynthesis

Matching Technology

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Guizhou, the second largest flue-cured tobacco producing area in China

1st Background

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1st Background

Low Temperature in Spring

(LI Y and Yang X G, 2010)

Guizhou is high incidence

area for late spring

chilling. Low temperature

in spring usually result in

lowly growing for tobacco

seedlings.

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Sunless Weather

Guizhou is located on the

yunnan-guizhou plateau, and

average sunshine time

between January and April is

only about 95 hours during

seedling. Flue-cured tobacco

is a light crop, therefore,

sunless weather would lead to

development retardation.

(Zheng Y and Zhao X B, 2002)

1st Background

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Spring Drought

Drought in spring is the main

meteorological disasters on

yunnan-guizhou plateau karst

area. Spring drought is the

disadvantage of transplanting

and survival for tobacco

seedlings.

1st Background

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Sunlight: Appropriate shading at seedling stage

can improve growing of tobacco seedlings.

(Wang et al., 2010)

Temperature: Plants grown at high temperature

showed higher electron flow devoted to

oxygenation of RuBP than plants grown at low

temperature. (Zhang et al., 2013)

Moisture: Effect of water stress on all the traits

related to yield, quality traits and all the traits

related to yield except numbers of leaves.

(Biglouei et al., 2010)

1st Background

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Field Experiment Pot Experiment

Combined with the field and pot experiments, it was to study on photosynthetic active

radiation (PAR) level, air temperature, soil evaporation, leaf water potential and to

analyze leaf photosynthesis and maximal photochemical efficiency (Fv/Fm) of

photosynthetic system II (PSII) in well-cellar style transplanting (WCST), compared

with conventional transplanting (CT).

2nd Changed Micro-environment

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Photosynthetic Active Radiation

CT：Conventional Transplanting

WCST：Well-cellar Style

Transplanting

4 cm below surface

8 cm below surface

281 μmol m-2 s-1：CT

204 μmol m-2 s-1 ：WCST}

316 μmol m-2 s-1：CT

167 μmol m-2 s-1 ：WCST}


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Air temperature (Infrared Thermal Imaging)

空

白

常

规 Max. 16.2℃, Min. 14.4℃,

Avg. 15.7℃ Max. 30.0℃, Min. 23.2℃,

Avg. 25.6℃ Max. 41.1℃, Min. 28.8℃,

Avg. 34.5℃

Max. 51.0℃, Min. 34.0℃,

Avg. 44.0℃

Max. 53.4℃, Min. 36.7℃,

Avg. 48.2℃

Max. 38.2℃, Min. 32.3℃,

Avg. 36.0℃

Max. 30.0℃, Min. 25.2℃,

Avg. 27.6℃

空

白

井

窖 Max. 18.9℃, Min. 14.9℃,

Avg. 17.3℃ Max. 29.4℃, Min. 20.9℃,

Avg. 21.9℃ Max. 38.3℃, Min. 22.1℃,

Avg. 25.1℃

Max. 45.9℃, Min. 29.4℃,

Avg. 34.0℃

Max. 53.0℃, Min. 33.3℃,

Avg. 38.0℃

Max. 35.7℃, Min. 31.4℃,

Avg. 33.3℃

Max. 31.9℃, Min. 26.3℃,

Avg. 29.0℃

CT

Max. 16.0℃, Min. 13.5℃,

Avg. 14.1℃ Max. 26.4℃, Min. 22.4℃,

Avg. 24.2℃

Max. 37.7℃, Min. 26.0℃,

Avg. 29.1℃

Max. 55.4℃, Min. 31.4℃,

Avg. 36.8℃

Max. 56.1℃, Min. 32.2℃,

Avg. 38.2℃

Max. 38.3℃, Min. 28.4℃,

Avg. 30.6℃

Max. 30.5℃, Min. 23.1℃,

Avg. 25.0℃

WCST

Max. 18.8℃, Min. 13.4℃,

Avg. 15.0℃ Max. 26.1℃, Min. 20.7℃,

Avg. 22.0℃

Max. 31.5℃, Min. 21.9℃,

Avg. 25.7℃

Max. 41.5℃, Min. 28.2℃,

Avg. 31.4℃

Max. 44.4℃, Min. 30.0℃,

Avg. 32.6℃

Max. 35.3℃, Min. 28.8℃,

Avg. 30.2℃

Max. 31.2℃, Min. 23.2℃,

Avg. 24.9℃

06-08 时 08-10 时 10-12 时 12-14 时 14-16 时 16-18 时 18-20 时

Delaying the response s of near-surface air temperature for WCST.

Higher temp. for WCST, when low temp. for CT；and be inverted.


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Temperature Values and Infrared Thermal Imaging

Daily Rainfall

20th: 10.6 mm

21st: 0.6 mm

A.M. 6 P.M. 14 P.M. 20

CT

WCST

Air temp. for WCST

from 14.13 ℃ to 30.52 ℃,

and Range 16.39 ℃; Air

temp. for CT from 11.74

℃ to 44.22 ℃, and Range

32.48 ℃.


Air temperature (Infrared Thermal Imaging) 2015

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Evaporation simulation system

Simulated evaporation 563 min (about 9.38

hours), and evaporation capacity was 2189.49 mm

for WCST and 2587.58 mm for CT.

Evaporation speed was 3.89 mm min-1 for

WCST and 4.60 mm min-1 for CT. Evaporation rate

for WCST was reduced by 18.25% than CT.

* Ambient temperature：20 ℃


Soil Surface Evaporation

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4.6 mm 2.8 mm

Relative Humidity

Soil Moisture

Relative humidity for

WCST ranged from

64.28% to 100%,

however, RH for CT

ranged from 25.71% to

97.75%.

Therefore, leaf water

potential for WCST was

always higher than CT.CT：常规移栽；WCST：井窖式移栽


Leaf Water Potential 2015

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Agronomy Traits

leaf

length

and

width

Height

SPAD

Tobacco seedlings can return rapidly for WCST than CT

3rd Seedlings Growth and Photosynthesis

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Dry root matter distribution for WCST and CT（g root/kg soil）

Dry root matter distribution for

WCST was ranged from 15 cm to

20 cm below soil surface, while for

CT mainly focused on 10 cm below

soil surface. The results were

accord with transplant depths


Root Distribution

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Total Root Length

Average Diameter

Root Surface Area

Total Root Volume


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CO2 response curve

Transplanting

methodsα

Pmaxμmolm-2 s-1

Rdμmolm-2 s-1

LSPμmolm-2 s-1

LCPμmolm-2 s-1

CT 0.045 19.41 4.33 1180.68 88.75

WCST 0.047 15.27 3.56 834.45 70.61

Light-response curve of photosynthesis

WCST

CT


Photosynthetic Characters 2015

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DAT 1 DAT 2 DAT 3 DAT 4

CT

WCST(DAT, Days after Transplanting)

Daily Fv/Fm

Fv/Fm=0.63

Fv/Fm=0.69

Fv/Fm=0.67 Fv/Fm=0.65 Fv/Fm=0.68

Fv/Fm=0.72 Fv/Fm=0.71 Fv/Fm=0.73


Maximal Photochemical Efficiency

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Different mulching films

a. Mulching films before transplanting（Better soil moisture）

Mulching Making well-cellar Putting seedling

Sealing

Watering

4th Matching Technology

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Poor soil moisture(Spring drought

area)

b. Transplanting before mulching films

Making well-cellar with watering

Putting seedling

WateringMulching filmReleasing seedling


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c. No mulching for WCST

Growing

Better soil

moisture


Making well-cellar with watering

Making well-cellar Putting seedling Watering

Poor soil m

oisture

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Tobacco seedlings standard

Tobacco seedlings for WCST：leaf-cutting times less than two times；Height

of tobacco seedlings ranged from 3 cm to 5 cm with 4 to 5 leaves.

Population and Individual


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Watering volume

SongtaoLonggang

Relative soil moisture (%)

Longgang（Test Area）

45% 65% 85%

Songtao（Produing Area）

30-50% 50-70% 70-90%

Watering volume （150-200）mL/cave （100-150）mL/cave 100 mL/cave


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Technique of industrialized seedling

CK RL

BL LL


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Treatment Total root length/cm

Surface area/cm2

Average diameter/mm

Total root volume/cm3

R4W8 55.53±5.04b 7.99±0.85b 0.47±0.03b 0.09±0.01b

B4W8 75.45±9.53a 13.49±0.90a 0.51±0.04ab 0.18±0.02a

L4W8 70.29±8.89a 7.40±1.26bc 0.48±0.04b 0.08±0.01b

CK 31.55±0.28c 6.03±0.69c 0.55±0.03a 0.09±0.02b


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Thanks for your attentions

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LIN YECHUN 2015 - CORESTA · LIN YECHUN 2015.09 2015_TSRC106_LinYechun.pdf TSRC2015(69) - Document...

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Transcript of LIN YECHUN 2015 - CORESTA · LIN YECHUN 2015.09 2015_TSRC106_LinYechun.pdf TSRC2015(69) - Document...