Limitation of animal models in drug discovery and development Candice Tahimic, Ph.D. Hence, there is...
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Limitation of animal models in drug discovery and development Candice Tahimic, Ph.D. Hence, there is a need to use human cells to test the efficacy of candidate drugs. Animal cells may not always simulate complex biochemical mechanisms of human disease Is the observed disease phenotype in the animal equivalent to that of the human counterpart?
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Transcript of Limitation of animal models in drug discovery and development Candice Tahimic, Ph.D. Hence, there is...
Limitation of animal models in drug discovery and development
Candice Tahimic, Ph.D.
Hence, there is a need to use human cells to test the efficacy of candidate drugs.
Animal cells may not always simulate complex biochemical mechanisms of human disease
Is the observed disease phenotype in the animal equivalent to that of the human counterpart?
Midbrain dopaminergic neurons in the substantia nigra are selectively lost in Parkinson’s disease
Human ES or iPS cells can be converted into dopaminergic neurons for use as cell models of Parkinson’s disease
Certain psychiatric disorders and certain addictions (nicotine and cocaine dependence) are thought to be mediated by the dopaminergic system
Candice Tahimic, Ph.D.
Human cell models for disease: the promise of hES/iPS cells
F
Test compound
Rep
orte
r le
vels
A
BC
DE
“On-dish” assay for efficacy
Chemical library screening
Dirrected differentiation
Candice Tahimic, Ph.D.
Human ES cell
Neurons
The iPS or hESC -derived neurons are treated with a number of candidate drugs for a particular disease. The neurons are then observed for reversal of the disease phenotype. Promising test compounds are further validated.
