Glucocorticoids Induce the Accumulation of Novel Angiotensinogen ...
Likelihood in crystallography/media/people/new-fellows-2014/Presentati… · Rob Oeffner, R (Miffy)...
Transcript of Likelihood in crystallography/media/people/new-fellows-2014/Presentati… · Rob Oeffner, R (Miffy)...
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Randy J Read Department of Haematology
Likelihood in crystallography
Known knowns and known unknowns
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Phasing by molecular replacement
• Phases can be calculated from atomic model • Rotate and translate related structure
amplitude
phase
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Known knowns…
• Donald Rumsfeld, 12 February 2002: • “There are known knowns; there are things we know
we know. We also know there are known unknowns, that is to say we know there are some things we do not know. But there are also unknown unknowns, the ones we don't know we don't know.”
Known knowns
Known unknowns
Unknown knowns
Unknown unknowns
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Patterson-based molecular replacement
• Patterson map shows vectors between atoms • computed without knowing phases
• Rotate/translate model to reproduce Patterson
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Probabilities in crystallography
• Diffraction: adding waves • as vectors or complex numbers
• Effect of errors • complex normal
distribution DF
F σΔ
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Likelihood-based molecular replacement in Phaser
• Likelihood target: • probability of observed amplitude
given structure factor contributions from model(s)
• Benefits of likelihood • account for expected size of errors in model • account for lack of completeness of model • exploit knowledge from partial solutions • allow ensemble of possible models
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Human angiotensinogen: molecular replacement
human
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Human angiotensinogen: molecular replacement
human
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Solving angiotensinogen structures
human
rat I
rat II
+ GdCl3
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S-‐S (18-‐138)
S-‐S (18-‐138)
Renin
Renin:angiotensinogen complex
Zhou, Carrell, Murphy, Wei, Yan, Stanley, Stein, Broughton Pipkin, Read (2010).
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Increasing statistical sophistication
• Crystals commonly suffer from translational non-crystallographic symmetry • multiple copies diffract in
and out of phase
Photo courtesy of Laurie Betts
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Increasing statistical sophistication
• Crystals commonly suffer from translational non-crystallographic symmetry • multiple copies diffract in
and out of phase
Hyp-1: Sliwiak, Jaskolski, Dauter, McCoy, Read (2014).
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Predicting signal-to-noise
• Signal-to-noise depends on: • the fraction of scattering accounted for by the model • expected RMSD of model • number of reflections
(size and resolution)
• Use this to: • predict what is possible • develop optimal strategies
LLG
σ A2
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Aldose reductase
• 36 kDa, 0.78Å resolution (3bcj) • Find 4 S (<3h) • Complete with
N atoms (3h) • 2525 non-H atoms
in structure • none heavier than S • new ab initio record?
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Acknowledgments
• Phaser • Airlie McCoy, Gábor Bunkóczi,
Rob Oeffner, R (Miffy) Mifsud, Rita Giordano
• Angiotensinogen • Robin Carrell, Aiwu Zhou, Penny Stein, Yahui Yan
• Hyp-1 • Mariusz Jaskolski, Joanna Sliwiak,
Zbyszek Dauter • Single-atom MR
• Airlie McCoy, Antoni Wrobel