Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal...

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Lighting the way. Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new choice for your qPCR and RT-qPCR. Luna products have been optimized for robust performance on diverse sample sources and target types. Available for dye-based or probe-based detection, Luna products can be used across a wide variety of instrument platforms. With so many qPCR and RT-qPCR options available, why not make a simpler, more cost-efective choice that delivers the sensitivity and precision you expect for your qPCR and RT-qPCR. Visit LUNAqPCR.com to request your sample today. One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may require you to obtain additional third party intellectual property rights for certain applications. be INSPIRED drive DISCOVERY stay GENUINE RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step RT-qPCR Kit over an 8-log range of input template concentrations (1 μg – 0.1 pg Jurkat total RNA) with 8 replicates at each concentration. Reaction setup and cycling conditions followed recommended protocols, including a 10-minute RT step at 55°C for the thermostable Luna WarmStart ® Reverse Transcriptase. NTC = non-template control NEB’s Luna Universal One-Step RT-qPCR Kit ofers exceptional sensitivity, reproducibility and RT-qPCR performance 1 μg 0.1 μg 10 ng 1 ng 0.1 ng 10 pg 1 pg 0.1 pg Input NTC Cycle ∆Rn 10 1 0.1 2 38 36 34 32 30 28 26 24 22 20 18 10 8 6 4 40 16 14 12 Amplification plot n=8

Transcript of Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal...

Page 1: Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new

Lighting the way.™

Introducing Luna® UniversalqPCR and RT-qPCR Products

New England Biolabs is pleased to introduce a

bright, new choice for your qPCR and RT-qPCR.

Luna products have been optimized for robust

performance on diverse sample sources and target

types. Available for dye-based or probe-based

detection, Luna products can be used across a wide

variety of instrument platforms. With so many

qPCR and RT-qPCR options available, why not

make a simpler, more cost-efective choice that

delivers the sensitivity and precision you expect for

your qPCR and RT-qPCR.

Visit LUNAqPCR.com to request your

sample today.

One or more of these products are covered by one or more patents, trademarks and/or copyrights owned or controlled

by New England Biolabs, Inc. For more information, please email us at [email protected]. The use of these products may

require you to obtain additional third party intellectual property rights for certain applications.

be INSPIRED

drive DISCOVERY

stay GENUINE

RT-qPCR targeting human GAPDH was performed using the Luna Universal One-Step

RT-qPCR Kit over an 8-log range of input template concentrations (1 μg – 0.1 pg Jurkat

total RNA) with 8 replicates at each concentration. Reaction setup and cycling conditions

followed recommended protocols, including a 10-minute RT step at 55°C for the thermostable

Luna WarmStart® Reverse Transcriptase. NTC = non-template control

NEB’s Luna Universal One-Step RT-qPCR Kit ofers exceptional

sensitivity, reproducibility and RT-qPCR performance

1 μg

0.1 μg

10 ng

1 ng

0.1 ng

10 pg

1 pg

0.1 pg

Input

NTC

Cycle

∆Rn

10

1

0.12 383634323028262422201810864 40161412

Amplification plot

n = 8

Page 2: Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new

Image:jim

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Page 3: Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new

new products

14 APRIL 2017 • VOL 356 ISSUE 6334 209 SCIENCE sciencemag.org/custom-publishing

LIFE SCIENCE TECHNOLOGIES Produced by the Science/AAAS Custom Publishing Office

DNA Blood Kit

The Arcis DNA Blood Kit

prepares DNA from blood

using a novel 3-minute, two-

step system. Designed for in

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kit is sold in a ready-to-use

format, and enables users to

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whole-blood samples without

the need for prior sample preparation steps. This kit is also suitable

for specimens that have been stored in ethylenediaminetetraacetic

acid (EDTA) and heparin-containing solutions. It is nonhazardous, and

ships and stores at room temperature for ease of handling. Designed

with point-of-care in vitro diagnostics in mind, DNA extracted using the

kit is also suitable for use in PCR or other applications. Where access

to instrumentation required for traditional extraction methods is not

practical, its practicality and ability to be shipped and stored at ambient

temperatures has big upsides for users in emerging markets.

Arcis Biotechnology

For info: 44-(0)-1925-607100

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Electronically submit your new product description or product literature information! Go to www.sciencemag.org/about/new-products-section for more information.

Newly offered instrumentation, apparatus, and laboratory materials of interest to researchers in all disciplines in academic, industrial, and governmental organizations are featured in this

space. Emphasis is given to purpose, chief characteristics, and availability of products and materials. Endorsement by Science or AAAS of any products or materials mentioned is not

implied. Additional information may be obtained from the manufacturer or supplier.

Universal CentrifugesThe UniCen M and UniCen MR centrifuges from Herolab are the

ideal choice for small-volume sample use. These microprocessor-

controlled models have been especially designed for tubes, bottles,

microtiter plates, microliter tubes, and tissue-culture tubes. The

maximum capacity of the UniCen is 4 mL x 200 mL. Features such as

overspeed detection, imbalance detection, and emergency lid-lock

release mechanisms ensure that the models conform to all safety

requirements. The stainless-steel chamber, which has an additional

inner guard ring, is robust and will provide years of trouble-free

service. With the UniCen range, you can chose from 13 angle rotors,

3 swing-out rotors, and 1 microhematocrit rotor. The speed can be set

between 500 rpm and 24,000 rpm. There are nine preprogrammed

acceleration and deceleration ramps available. An easy-to-read LCD

display indicates both current and preset values of speed/g-force,

time, and temperature in the case of the UniCen MR, which is

refrigerated.

Herolab

For info: 49-(0)-6222-5802-0

www.herolab.de/index.php/en

ABI Analyzer Accessories&'()�������)��'�)�'���')���) �')�����)�������)�����)�����)

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a capillary array regeneration service so that you can increase the

number of usages from your capillary arrays. If you are using the

�! )����"�������)�! )����"�������)��)�! )����"�������)���(�)'��)

us your expired array and we will regenerate it back to its full use for

just a fraction of the cost of a brand new array. This service comes

with the same warranty as a new array. We will return the array to you

clean and dry, suitable for storage, and ready for use. Arrays can be

regenerated multiple times. Check our website or catalog for the full

range of accessories suitable for Applied Biosystems (ABI) analyzers.

��'�')��#)$�(()�(�)%��)�� �# ���)��)��$) �)�' #��)�����)���)

regeneration.

Gel Company/Aplegen

For info: 415-247-8760

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Wnt Signaling Pathway Products��� ���� ������ �������� ����� �������� ������� ��� ����

signaling research, including recombinant proteins, stable reporter

cell lines, polyclonal antibodies, and ELISA assay kits. Wnt proteins

are a family of cysteine-rich secreted polypeptides involved in

important cell functions including cell-to-cell communication, as

well as cell proliferation, migration, polarity, survival, and self-

renewal. In particular, Wnt3a signals play an important role in the

ability of organoids to expand. Additionally, loss of activation of

Wnt expression has been associated with alteration of cell fate,

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in developmental biology and oncology research. Wnt human and

mouse recombinant proteins are available in high (85%ñ90%) and low

(75%) purities. The HEK293, HCT-116, or SW480 Wnt stable cell lines

all have available matching controls. Polyclonal Wnt antibodies are

produced in vivo and are suitable for ELISA, immunohistochemistry,

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ELISA assay kits.

AMS Biotechnology

For info: 44-(0)-1235-828200

www.amsbio.com

Refrigerated IncubatorsHeratherm refrigerated incubators provide a consistent temperature

environment for incubation applications in the range of 5∞C to 70∞C, with

enhanced accuracy from 15∞C to 25∞C. The instrumentsí easy-to-use in-

terface can store up to 10 temperature protocols set by the user, with up

to 10 steps in each, and includes an advance timer function, an automatic

temperature alarm, and adjustable overtemperature protection. They

employ Peltier technology, circumventing the need for a refrigerant by

using a thermoelectric element to heat or cool in one module. In addition

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chemicals that are either phased out or are being phased out by the U.S.

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ous functions, including microbiology/fungi/yeast studies, reagent and

antibody storage, and shelf-life testing.

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For info: 800-955-6288

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ence materials (CRMs) for analytical spectroscopy and chromatography.

We have a full line of AA, IC, ICP, ICP/MS, GC, GC/MS, HPLC, and LC/MS

standards, in addition to many other products that are used in laborato-

ries around the world. Our product lines include standards for all of the

most commonly used environmental testing methods. Our standards are

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scope of accreditations is the most comprehensive in the industry.

SPEX CertiPrep

For info: 800-522-7739

www.spexcertiprep.com

Page 4: Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new

AAASmembers harness the power of scientifc

thinking to help tackle complex, global challenges.

Join the community of scientists, engineers,

and educators whose work impacts millions.

How will YOUmake your mark in science?

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www.aaas.org/join

A M E R I C A N A S S O C I A T I O N F O R T H E A D V A N C E M E N T O F S C I E N C E

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Page 5: Lighting the way. - Science · 2018-02-16 · Lighting the way. ™ Introducing Luna ® Universal qPCR and RT-qPCR Products New England Biolabs is pleased to introduce a bright, new

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