25/01/2012

1

Lecture 9 Animal Cell Biotechnology

Scaling up the production process

Marine impeller

Lecture 9 Animal Cell Biotechnology

Scaling up the production process

Motor driven magnetic stirrer. Removable motor that fits on top of the drive shaft, removed before washing and autoclaving.

Lecture 9 Animal Cell Biotechnology

Scaling up the production process

Temperature

• thermal probe inserted through headplate into culture

• maintain temperature at ~37oC ± 0.5oC

→ use heating elements (may have localized overheating at low agitation speeds)

→ heated water (37oC) circulating around an outer “jacket”, or through coiled pipes within bioreactor

→ for small scale reactors may also use electric heating jacket that wraps around the vessel

Lecture 9 Animal Cell Biotechnology

Scaling up the production process

Electric Heating jacket

Heating probe that fits into a shaft that goes into the medium. Probe itself isn’t in contact with the medium.