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Lecture 5 Bacterial Spores

Dr. Dimos

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Spore-forming Bacteria: Food

Industry

ore-forming Toxin Producers:lostridum botulinumlostridum perfringensacillus cereus

Spore-forming Food SpoilaAlicyclobacillusGeobacillusSporolactobacillus

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Clostridium botulinum

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Bacterial Spore Production

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Spore Structure

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Dipicolinic acidDP!"

Setlo# et. al. $%%&

DPA-less spores: lowerresistance to wet heat,dry heat, hydrogen

peroxide, and

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Targeted 'ene (noc) out *y+omologous ,ecom*ination

CSLD'enomic CSLD'enomic Target Site

CSLDCSLD Selection

CSLDCSLD Selection

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Small acid-solu*le proteins